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        Antibiotic resistance, biochemical typing, and PFGE typing of Bifidobacterium strains commonly used in probiotic health foods

        Xu, Feili,Wang, Junping,Guo, Yunchang,Fu, Ping,Zeng, Huawei,Li, Zhigang,Pei, Xiaoyan,Liu, Xiumei,Wang, Shuo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2

        This study firstly analyzed the antibiotic resistance, biochemical typing, and pulsed-field gel electrophoresis typing of 45 Bifidobacterium strains commonly used in health foods. Most strains were resistant to antibiotics but their antibiotic resistance rates were not high: Fos (56.52%), TET (43.48%), CRO (21.74%), AMC (15.22%), GEN (13.04%), RIF (10.87%), CHL (8.7%), CTX (6.52%), VAN (4.35%), and ERY (4.35%). The 45 strains could be divided into 14 pulsed-field gel electrophoresis types, of which the strain numbers of six pulsed-field gel electrophoresis types were more than one. All the Bifidobacterium strains could be divided into nine types by API50CHL biochemical identification. The same species displayed same biochemical typings, expect for B. animalis. Furthermore, the results confirmed that the same pulsed-field gel electrophoresis-type strains had closer antibiotic resistance patterns, and the same biochemical-type strain also had similar antibiotic resistance patterns.

      • KCI등재

        Antibiotic resistance, biochemical typing, and PFGE typing of Bifidobacterium strains commonly used in probiotic health foods

        Feili Xu,Junping Wang,Yunchang Guo,Huawei Zeng,Ping Fu,Zhigang Li,Xiaoyan Pei,Xiumei Liu,Shuo Wang 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2

        This study firstly analyzed the antibiotic resistance, biochemical typing, and pulsed-field gel electrophoresis typing of 45 Bifidobacterium strains commonly used in health foods. Most strains were resistant to antibiotics but their antibiotic resistance rates were not high: Fos (56.52%), TET (43.48%), CRO (21.74%), AMC (15.22%), GEN (13.04%), RIF (10.87%), CHL (8.7%), CTX (6.52%), VAN (4.35%), and ERY (4.35%). The 45 strains could be divided into 14 pulsed-field gel electrophoresis types, of which the strain numbers of six pulsed-field gel electrophoresis types were more than one. All the Bifidobacterium strains could be divided into nine types by API50CHL biochemical identification. The same species displayed same biochemical typings, expect for B. animalis. Furthermore, the results confirmed that the same pulsed-field gel electrophoresis-type strains had closer antibiotic resistance patterns, and the same biochemicaltype strain also had similar antibiotic resistance patterns.

      • KCI등재

        Experimental investigation of novel pre-tightened teeth connection technique for composite tube

        Fei Li,Qilin Zhao,Haosen Chen,Longxing Xu 국제구조공학회 2017 Steel and Composite Structures, An International J Vol.23 No.2

        A new composite tube connection method called the pre-tightened teeth connection technique is proposed to improve the composite tube connection efficiency. This paper first introduces the manufacturing process of the proposed technique. It then outlines how the mechanical properties of this technology were tested using four test groups. The factors that influence the load-bearing capacity and damage model of the connection were analyzed, and finally, the transfer load mechanism was investigated. The following conclusions can be obtained from the research results. (1) The new technique improves the compressive connection efficiency by a maximum of 79%, with the efficiency exceeding that of adhesive connections of the same thickness. (2) Changing the depth of teeth results in two types of damage: local compressive damage and shear damage. The bearing capacity can be improved by increasing the depth, length, and number of teeth as well as the pretightening force. (3) The capacity of the technique to transfer high loads is a result of both the relatively high interlaminar shear strength of the pultruded composite and the interlaminar shear strength increase provided by the pre-tightening force. The proposed technique shows favorable mechanical properties, and therefore, it can be extensively applied in the engineering field.

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