Investigation on the Biodegradability and Antibacterial Properties of Nanohybrid Suture Based on Silver Incorporated PGA-PLGA Nanofibers

Farzaneh Rouhollahi,Seyed Abdolkarim Hosseini,Farzaneh Alihosseini,Alireza Allafchian,Fatemeh Haghighat 한국섬유공학회 2018 Fibers and polymers Vol.19 No.10

Polyglycolic acid-poly lactic glycolic acid (PGA-PLGA) electrospun nanofibers containing silver nanoparticles have been produced and twisted into the nanofibrous yarn. The morphology of nanofibers and produced yarns, as well as the mechanical properties of the yarns, were investigated. Furthermore, in vitro antibacterial properties and in vitro degradation behavior of yarns containing various silver nanoparticles were studied. SEM images confirmed that the addition of the silver nanoparticles into the polymer solution increases the fiber diameters. The result of the mechanical test of the yarns alone and used in two different forms of the knots was measured and results showed that the strength of the yarns without the knot was significantly more than that of others. The biodegradability test showed that the mechanical properties and the weight of the yarns were quickly reduced after subjecting to in vitro condition. The result of the antibacterial test indicated that the nanofiber yarns containing %3 silver nanoparticles were the most appropriate sample with a considerably antibacterial activity against both gram-positive bacterium Staphylococcus aureus and gram-negative bacterium Escherichia Coli with inhibition zones of 8.1 and 9.5 mm, respectively; which demonstrated that silver nanoparticles retained their effectiveness after the electrospinning process. Therefore the nanofibrous yarns containing silver nanoparticles could be successfully produced by the electrospinning process with the proper antibacterial property as a candidate for the surgical sutures.

Effects of combined elicitors on parthenolide production and expression of parthenolide synthase (TpPTS) in Tanacetum parthenium hairy root culture

Farzaneh Pourianezhad,Hassan Rahnama,Amir Mousavi,Mahmood Khosrowshahli,Sudabeh Mafakheri 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.3

Feverfew (Tanacetum parthenium) is a medicinal herb that has multiple pharmacologic properties. This plant is rich parthenolide (PN). Hairy roots (HRs) culture is a valuable biotechnological tool for the production of plant secondary metabolites. Furthermore, application of elicitors is one of the most effective strategies to induce the metabolite biosynthesis. This study has investigated the effects of various elicitors on the production of PN in feverfew hairy root culture and the expression of parthenolide synthase (TpPTS) gene. Three elicitors were used separately and in combination, including yeast extract (YE) (2.5 mgL− 1), methyl jasmonate (MJ) (100 μM), Ag+ (100 μM), YE + MJ, Ag+ + MJ, YE + Ag+, and YE + MJ + Ag+ which were added to the 1/2MS medium on day 18 and after 48 h and 96 h, hairy roots were collected to determine the PN content. Total RNA was extracted from hairy roots and used for cDNA synthesis and real-time PCR analysis to investigate the gene expression of TpPTS. The PN content was determined by HPLC. The highest PN content (0.05 mg g− 1 dry weight) was achieved after application of elicitors (in 2.5 mgL− 1 YE and 100 μM MJ) and the highest expression of TpPTS gene also occurred after 96 h in the same treatment. It was concluded that some of the combined elicitors could efficiently increase the parthenolide content of feverfew in vitro culture. The additive effect of elicitors might be due to the separate mechanisms of each substance that is acting in an independent pathway.

Four phase hydrate equilibria of methane and carbon dioxide with heavy hydrate former compounds: Experimental measurements and thermodynamic modeling

Farzaneh Feyzi,Hamed Tavasoli 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.8

We experimentally investigated the hydrate dissociation condition for four phase hydrate (H)-aqueous liquid (LAq)-hydrocarbon rich liquid (LHC)-vapor (V) for the ternary systems of help gas-heavy hydrate former-water. Methane and carbon dioxide are known as help gases and benzene and cyclohexane are considered as heavy hydrate formers. The experimental data were generated using an isochoric pressure-search method. Two different equations of state (EOS) were employed to study the equilibrium phase behavior of ternary four phase systems. The EOSs considered are Valderama-Patel-Teja EOS combined with non-density dependent mixing rule (VPT+NDD) and Statistical Associating Fluid Theory EOS proposed by Huang and Radosz (SAFT-HR). The required binary interaction parameters (BIP) were obtained using vapor-liquid equilibrium (VLE) and liquid-liquid equilibrium (LLE) data. The hydrate phase was modeled by the modification of the solid solution theory of van der Waals and Platteeuw. To obtain reliable results, distortion of cages due to occupation of large molecules was considered. The Kihara parameters of cyclohexane were adjusted to hydrate dissociation data. Model calculations for hydrate forming conditions were found to be in satisfactory agreement with the newly reported data in this work and literature data.

Effects of Salt Stress on Physiological and Postharvest Quality Characteristics of Different Iranian Genotypes of Basil

Farzaneh Bekhradi,Mojtaba Delshad,Alicia Marín,Maria C Luna,Yolanda Garrido,Abdolkarim Kashi,Mesbah Babalar,Maria I Gil 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.6

This study examined the effects of salt stress on the physiological responses and postharvest quality characteristics of fresh sweet basil (Ocimum basilicum L.). Plants were treated with 40 and 80 mM NaCl for 25 days during cultivation in 2014. Two green genotypes, Green Iranian and Genovese basil, were studied after harvest, and while two Iranian genotypes, Green and Purple Iranian basil, were compared during storage. Yield and the stem length were significantly reduced under salt stress only in the two Iranian cultivars. Salinity significantly reduced leaf thickness and the area of parenchyma cells in both Green Iranian and Genovese basil. As salt concentration increased, transpiration and the content of chlorophylls decreased significantly while the lipid peroxidation increased in the Genovese genotype. During storage at 12°C, the respiration rate of salt-stressed leaves was similar to that of control leaves. The visual quality of Purple Iranian basil was better preserved during storage than the Green Iranian basil. However, salt stress positively affected the visual quality of Green Iranian basil, decreasing darkening and maintaining the quality above the limit of marketability after 7 days of storage. The total content of phenolic acids and anthocyanins did not show significant differences between growth conditions. However, during storage of Green Iranian basil, salt stress increased the content of individual and total phenolic acids compared to the control. To summarize, the positive or negative effects of salt stress on basil depend on the degree of tolerance of the different genotypes.

Comparing type-1, interval and general type-2 fuzzy approach for dealing with uncertainties in active control

Farzaneh Shahabian Moghaddam,Hashem Shariatmadar 국제구조공학회 2023 Smart Structures and Systems, An International Jou Vol.31 No.2

Nowadays fuzzy logic in control applications is a well-recognized alternative, and this is thanks to its inherent advantages. Generalized type-2 fuzzy sets allow for a third dimension to capture higher order uncertainty and therefore offer a very powerful model for uncertainty handling in real world applications. With the recent advances that allowed the performance of general type-2 fuzzy logic controllers to increase, it is now expected to see the widespread of type-2 fuzzy logic controllers to many challenging applications in particular in problems of structural control, that is the case study in this paper. It should be highlighted that this is the first application of general type-2 fuzzy approach in civil structures. In the following, general type-2 fuzzy logic controller (GT2FLC) will be used for active control of a 9-story nonlinear benchmark building. The design of type-1 and interval type-2 fuzzy logic controllers is also considered for the purpose of comparison with the GT2FLC. The performance of the controller is validated through the computer simulation on MATLAB. It is demonstrated that extra design degrees of freedom achieved by GT2FLC, allow a greater potential to better model and handle the uncertainties involved in the nature of earthquakes and control systems. GT2FLC outperforms successfully a control system that uses T1 and IT2 FLCs.

Application of Tumor Markers SCC-Ag, CEA, and TPA in Patients with Cervical Precancerous Lesions

Farzaneh, Farah,Shahghassempour, Shapour,Noshine, Bahram,Arab, Maliheh,Yaseri, Mehdi,Rafizadeh, Mitra,Alizadeh, Kamyab Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Background: To determine the potential clinical utility of tumor markers CEA, TPA, and SCC-Ag for early detection of cervical precancerous lesions. Materials and Methods: A case-control study was carried out on 120 women (46 patients with histologically confirmed cervical precancerous lesions and 74 healthy controls). The significance of serum selected tumor markers in early detection of cervical intraepithelial neoplasia (CIN) were assessed. Results: Of the case group, the rates of CIN I, II, III, was 69.6%, 23.9%, and 6.5%, respectively. According to the manufacturer's cut-off values of 2ng/ml, 5ng/ml, and 70 U/ml for SCC-Ag, CEA and TPA tests, in that order, SCC-Ag test had a sensitivity of 13%, but CEA and TPA tests could not distinguish between case and control groups. The diagnostic sensitivities were highest at cut-off values of 0.55 ng/ml for SCC-Ag, 2.6ng/ml for CEA, and 25.5 U/ml for TPA which were 93%, 61%, and 50%, respectively. However, the area under the receiver operating characteristic curve was the largest for SCC-Ag (0.95 vs. 0.61 and 0.60 for CEA and TPA, respectively). Moreover, there was a highly significant direct correlation between SCC-Ag concentration and the degree of cervical precancerous lesions (r=0.847, p<0.001). Conclusions: The new cutoff of 0.5 for SCC-Ag test might be useful as a tumor marker in Iranian patients with CIN and it needs to be more evaluated by studies with larger populationa.

An unusual cause of urinary incontinence: Urethral coitus in a case of Mayer-Rokitansky-Kuster-Hauser syndrome

Farzaneh Sharifiaghdas,Azar Daneshpajooh,Samira Sohbati,Mahboubeh Mirzaei 대한비뇨의학회 2016 Investigative and Clinical Urology Vol.57 No.5

Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome is a rare anomaly in women and is characterized by congenital aplasia of the uterus and vagina, with normal development of secondary sexual characteristics and a normal karyotype. We report a case of a 38-year-old women with MRKH syndrome that had experienced urethral sex for many years. She presented with urinary incontinence and dyspareunia. The patient's secondary sexual characteristics were normal, and examination revealed a widely open incompetent megalourethra and an absent vagina. Laboratory studies confirmed a 46, XX karyotype. Imaging included ultrasonography and magnetic resonance imaging, which indicated bilateral normal ovaries and a rudimental bicornuate uterus. After confirming the diagnosis of MRKH, the patient underwent urethroplasty by urethral plication, antiincontinence surgery by autologous fascial sling of the bladder neck, and the creation of a neo-vagina using a urethral flap. After 3 months, voiding cystourethrography and uroflowmetry confirmed normal voiding. There were no postoperative symptoms of urinary incontinence, and the patient was completely satisfied.

A Review of Pharmacological Treatments for Vaginal Atrophy in Postmenopausal Women in Iran

Farzaneh Rashidi Fakari,Masoumeh Simbar,Maryam Beheshti Nasab,Masoumeh Ghazanfarpour,Fahimeh Rashidi Fakari 대한폐경학회 2020 대한폐경학회지 Vol.26 No.2

Vaginal atrophy is one of the most common menopausal complications and is often overlooked. There are various pharmacological and non-pharmacological treatment approaches to reduce vaginal atrophy; however, no comprehensive study on a convenient, affordable, inexpensive, and noninvasive treatment with fewer complications has been conducted so far. Thus, the current study aimed to provide a systematic review of pharmacological treatment for vaginal atrophy in postmenopausal women in Iran. In this systematic review, all Iranian articles published in Persian or English during 2009 to 2019 were collected and analyzed by searching the Scopus, PubMed, Web of Science, Magiran, Iranian Registry of Clinical Trials (IRCT), and Cochrane Library databases. The inclusion criteria were clinical trials for vaginal atrophy and menopause. Based on the selection criteria, articles with a Jadad scale score of 3 and above were included in the study and qualitatively analyzed. Overall, 15 clinical trials met the inclusion criteria. In total, 12 articles examined the efficacy of pharmacological treatments (including three herbal medicines, three vitamins and dietary supplements, and two chemical drugs) in treating vaginal atrophy in postmenopausal women. Various types of medication have been used to improve vaginal atrophy, and effective treatments include licorice, chamomile, royal jelly, vitamin E, vitamin D, hyaluronic acid, and Vagifem; however, the results of studies on fennel have been inconsistent. However, considering the small number of studies reviewed, further studies with a stronger methodology are needed to confirm the efficacy of these medications.

Association of Estrogen Receptor α Genes PvuII and XbaI Polymorphisms with Type 2 Diabetes Mellitus in the Inpatient Population of a Hospital in Southern Iran

Farzaneh Mohammadi,Mohammad Pourahmadi,Mohadeseh Mosalanejad,Houshang Jamali,Mohamed Amin Ghobadifar,Saeideh Erfanian 대한당뇨병학회 2013 Diabetes and Metabolism Journal Vol.37 No.4

Background: Estrogen plays a fundamental role in the pathogenesis of type 2 diabetes mellitus (T2DM). Very few studies have shown the association between estrogen receptor α (ERα), PvuII and XbaI gene polymorphisms with T2DM in both men and women. We evaluated the hypothesis that PvuII and XbaI polymorphisms of ERα gene may be associated with T2DM in adult. Methods: From spring of 2010 to the fall of 2011, a case-control study was performed at clinical centers of Jahrom University of Medical Sciences. We included 174 patients with T2DM including men and women and 174 age, sex, and body mass index frequency-matched health controls. We analyzed the PvuII and XbaI polymorphisms of ERα by using the polymerase chain reaction-based restriction fragment length polymorphism method. Results: No significant differences between demographic characteristics of control and patients groups were observed. Allele frequencies of both PvuII and XbaI polymorphisms were significantly different between patients and control subjects (P=0.014vs. P=0.002, respectively). When the group was separated into women and men, logistic regression analysis of genotype distribution of PvuII (pp vs. Pp+PP) in both sexes revealed that there was no significant association of PvuII genotype with men (odds ratio [OR], 1.67; confidence interval [CI], 0.86 to 3.28; P=0.89) and women (OR, 0.96; CI, 0.53 to 1.74; P=0.12). Conclusion:PvuII and XbaI polymorphisms in ERα are related with T2DM in the inpatient population.

Sensory and Motor Behavior Evidences Supporting the Usefulness of Conditioned Medium from Dental Pulp-Derived Stem Cells in Spinal Cord Injury in Rats

Farzaneh Dehghani,Reza Asadi-Golshan,Vahid Razban,Esmaeil Mirzaei,Abdolkarim Rahmanian,Sahar Khajeh,Zohreh Mostafavi-Pour 대한척추외과학회 2018 Asian Spine Journal Vol.12 No.5

Study Design: Experimental animal study. Purpose: This study aimed to assess effects of conditioned medium (CM) of dental pulp-derived stem cells loaded in collagen hydrogel on functional recovery following spinal cord injury (SCI). Overview of Literature: SCI affects sensory and motor functions, and behavioral recovery is the most essential purpose of therapeutic intervention. Recent studies have reported that CM from dental pulp-derived stem cells has therapeutic benefits. In addition, collagen hydrogel acts as a drug delivery system in SCI experiments. Methods: Stem cells from human exfoliated deciduous teeth (SHEDs) were cultured, and SHED-CM was harvested and concentrated. Collagen hydrogel containing SHED-CM was prepared. The rats were divided into five groups receiving laminectomy, compressive SCI with or without intraspinal injection of biomaterials (SHED-CM), and collagen hydrogel with or without SHED-CM. Basso, Beattie, and Bresnahan (BBB) scoring, inclined plane, cold allodynia, and beam walk tests were performed for 6 weeks to assess locomotor, motor, sensory, and sensory-motor performances, respectively. Results: Scores of the rats receiving SHED-CM loaded in collagen hydrogel were significantly better than those of the other injured groups at 1-week post-injury for BBB, 2 weeks for inclined plane, 2 weeks for cold allodynia, and 4 weeks for beam walk tests (p <0.05). The differences remained significant throughout the study. Conclusions: Intraspinal administration of SHED-CM loaded in collagen hydrogel leads to improved functional recovery and proposes a cell-free therapeutic approach for SCI.