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- 저자 : Farina Mustaffa-Kamal (검색결과 1 건)
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Megat Hamzah Megat Mazhar Khair,Gayathri Thevi Selvarajah,Abdul Rahman Omar,Farina Mustaffa-Kamal 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.2
Background: The role of Toll-like receptors (TLRs) in a feline infectious peritonitis virus(FIPV) infection is not completely understood. Objectives: This study examined the expression of TLR3, TLR7, TLR9, tumor necrosis factoralpha(TNF-α), interferon (IFN)-β, and interleukin (IL)-10 upon an FIPV infection in Crandell-Reese feline kidney (CRFK) cells and feline monocytes. Methods: CRFK cells and monocytes from feline coronavirus (FCoV)-seronegative cats andFCoV-seropositive cats were infected with type II FIPV-79-1146. At four, 12, and 24 hours postinfection(hpi), the expression of TLR3, TLR7, TLR9, TNF-α, IFN-β, and IL-10, and the viralload were measured using reverse transcription quantitative polymerase chain reaction. Viralprotein production was confirmed using immunofluorescence. Results: FIPV-infected CRFK showed the upregulation of TLR9, TNF-α, and IFN-βexpression between 4 and 24 hpi. Uninfected monocytes from FCoV-seropositive cats showedlower TLR3 and TLR9 expression but higher TLR7 expression compared to uninfectedmonocytes from FCoV-seronegative cats. FIPV-infected monocytes from FCoV-seropositivecats downregulated TLR7 and TNF-α expression between 4 and 24 hpi, and 4 and 12 hpi,respectively. IFN-β was upregulated early in FIPV-infected monocytes from FCoV-seropositivecats, with a significant difference observed at 12 hpi compared to FCoV-seronegative cats. The viral load in the CRFK and FIPV-infected monocytes in both cohorts of cats was similarover time.ConclusionTLR7 may be the key TLR involved in evading the innate responseagainst inhibiting TNF-α production. Distinct TLR expression profiles between FCoVseronegativeand FCoV-seropositive cats were observed. The associated TLR that plays a rolein the induction of IFN-β needs to be explored further.
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