Identification of Mouse MARVELD1 as a Microtu-bule Associated Protein that Inhibits Cell Cycle Progression and Migration

Fanli Zeng,Yanyan Tian,Shuliang Shi,Qiong Wu,Shanshan Liu,Hongxia Zheng,Lei Yue,Yu Li 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.3

MARVEL domain-containing 1 (MARVELD1) is a newly identified nuclear protein; however its function has not been clear until now. Here, we report that mouse MARVELD1(mMARVELD1), which is highly conserved between mice and humans, exhibits cell cycle-dependent cellular localization. In NIH3T3 cells, MARVELD1 was observed in the nucleus and at the perinuclear region during interphase,but was localized at the mitotic spindle and midbody at metaphase, and a significant fraction of mMARVELD1translocated to the plasma membrane during anaphase. In addition, treatment of cells with colchicine, a microtubuledepolymerizing agent, resulted in translocation of mMARVELD1to the plasma membrane, and association of mMARVELD1 and α-tubulin was confirmed by co-immunoprecipitation. Finally, overexpression of mMARVELD1 resulted in a remarkable inhibition of cell proliferation, G1-phase arrest, and reduced cell migration. These findings indicate that mMARVELD1 is a microtubule-associated protein that plays an important role in cell cycle progression and migration.

Design of a UWB Antenna with Band-Notch Function

Zeng Fanli,Jaewon Lee,Chulhee Kim,Jaehoon Choi 한국전자파학회JEES 2010 Journal of Electromagnetic Engineering and Science Vol.10 No.1

In this paper, a compact wideband antenna with a band-notch function is proposed. It operates over the UWB band with a band-stop characteristic. To increase the impedance bandwidth, a ring-shaped radiator is used. By attaching a circular stub to the ring-shaped radiator, the band-notch performance is obtained. The proposed antenna operates over a frequency range from 2.7 ㎓ to 11 ㎓ to satisfy the 10-㏈ return-loss requirement and provides band-stop performance in the frequency band from 5.15 ㎓ to 6.1 ㎓. Experimental results reveal that the proposed antenna exhibits good radiation performance and is suitable for UWB applications.

IL-35 Over-expression is Associated with Genesis of Gastric Cancer

Fan, Yong-Gang,Zhai, Jing-Ming,Wang, Wei,Feng, Bing,Yao, Guo-Liang,An, Yan-Hui,Zeng, Chao Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

Overexpression of interleukin (IL)-35 has been found in a variety of malignancies, but the expression status in gastric cancer has yet to be elucidated clearly. In the present study, positive expression of EBI3 and p35 was 63.3% and 70.0% of cases, respectively. EBI3 expression was strongly related with larger tumor size and invasion depth (P<0.05). Similarly, expression of p35 was also correlated with larger tumor size (P<0.05). These results indicate that IL-35 might be involved in growth of gastric cancer. Interestingly, EBI3 and p35 expressions were positive correlated with Ki-67 expression. Moreover, EBI3 immunoreactivity was associated with Bcl-2 staining. Our data suggest IL-35 is correlated with genesis of gastric cancer by regulating growth and apoptosis.

Preparation and evaluation of enrofloxacin microspheres and tissue distribution in rats

Fan Yang,Jijun Kang,Fang Yang,Zhensheng Zhao,Tao Kong,Zhenling Zeng 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.2

New enrofloxacin microspheres were formulated, and their physical properties, lung-targeting ability, and tissue distribution in rats were examined. The microspheres had a regular and round shape. The mean diameter was 10.06 μm, and the diameter of 89.93% of all microspheres ranged from 7.0 μm to 30.0 μm. Tissue distribution of the microspheres was evaluated along with a conventional enrofloxacin preparation after a single intravenous injection (7.5 mg of enrofloxacin/kg bw). The results showed that the elimination half-life (t1/2β) of enrofloxacin from lung was prolonged from 7.94 h for the conventional enrofloxacin to 13.28 h for the microspheres. Area under the lung concentration versus time curve from 0 h to ∞ (AUC0-∞) was increased from 11.66 h·μg/g to 508.00 h·μg/g. The peak concentration (Cmax) in lung was increased from 5.95 μg/g to 93.36 μg/g. Three lung-targeting parameters were further assessed and showed that the microspheres had remarkable lung-targeting capabilities.

Molecular features and functional studies of transcription factor, Cap ‘n’ collar C, in the brown planthopper, Nilaparvata lugens Stål (Hemiptera Delphacidae)

Zeng Bin,Feng Ze-Rui,Liu Ya-Ting,Chen Fu-Rong,Wang Shuai,Wu Shun-Fan,Yu Zhi-Tao,Gao Cong-Fen 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.4

Cap ‘n’ collar C (CncC), a transcription factor, plays a vital role in the development of insect resistance by regulating the expression of multiple detoxifying genes. Clarifying the molecular characteristics of CncC and its pathway regulating insecticide resistance will aid the development of integrated pest control strategies. Here we cloned and identified the molecular feature of CncC genes from the brown planthopper (BPH), Nilaparvata lugens (Stål), (Hemiptera Delphacidae). The full-length open reading frame of NlCncC was 3015 nucleotides with 1005 amino acids. The deduced amino acid sequence has a high similarity with other insect homologs and contains the characteristic Cnc/bZip domain architecture. Functional studies showed that silencing NlCncC by RNA interference (RNAi) could downregulate the transcript expression of NlCYP6ER1, NlCYP6CW1, and NlCYP6AY1, which increase the susceptibility to imidacloprid in N. lugens. The identification of molecular characteristics of NlCncC and func tional studies in this paper will contribute to designing effective control strategies for N. lugens.

Bidirectional two-sample mendelian randomization analysis identifies causal associations between age at menarche and sarcopenia-related traits

Fan Wei,Gu Peng,Pu Bin,Yue Dan,Xin Qiao,Zheng XiaoHui,Zeng ZhanPeng,Fan Shaoyong 한국유전학회 2023 Genes & Genomics Vol.45 No.8

Background Observational studies have shown that the age of menarche is associated with sarcopenia, but confounding factors make the causal relationship difficult to infer. Objective Therefore, we conducted a bidirectional two-sample Mendelian randomized (MR) analysis to evaluate the potential causal relationship between age at menarche and sarcopenia-related traits (hand grip strength, lean mass, walking pace). Methods We obtained the latest aggregate statistics from the Genome-wide association studies (GWAS) database on the age of menarche of 182,416 participants from ReproGen, the appendicular lean mass of 244,730 participants from EMBL’s European Bioinformatics Institute, the left-hand grip strength of 401,026 participants, the right-hand grip strength of 461,089 participants and the usual walking pace of 459,915 participants from the UK Biobank. The inverse variance weighting (IVW) method and other MR methods were used to evaluate the bidirectional causal relationship between the age of menarche and sarcopenia. Results The forward MR results showed that the age of menarche predicted by the gene was positively correlated with left-hand grip strength (IVWβ=0.041, P = 2.00 × 10−10), right-hand grip strength (IVWβ=0.053, P = 1.97 × 10−18), appendicular lean mass (IVWβ=0.012, P = 4.38 × 10−13) and usual walking pace (IVWβ=0.033, P = 1.62 × 10−8).In the reverse MR analysis, we also found that the usual walking pace was positively correlated with the age of menarche predicted by genes (IVWβ=0.532, P = 1.65 × 10−4). Still, there was no causal relationship between grip strength and appendicular lean mass and the age at menarche. Conclusion Our results show that earlier menarche will increase the risk of sarcopenia. In addition, people with higher muscle function tend to have menarche later. These findings may provide a reference for prevention strategies and interventions for menarche in advance and sarcopenia.

Bioinformatics Analysis Reveals Connection of Squamous Cell Carcinoma and Adenocarcinoma of the Lung

Fan, Wei-Dong,Zhang, Xian-Quan,Guo, Hui-Lin,Zeng, Wei-Wei,Zhang, Ni,Wan, Qian-Qian,Xie, Wen-Yao,Cao, Jin,Xu, Chang-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Squamous cell carcinoma and adenocarcinoma are the major histological types of non-small cell lung cancer. Because they differ on the basis of histopathological and clinical characteristics and their relationship with smoking, their etiologies may be different; for example, different tumor suppressor genes may be related to the genesis of each type. We used microarray data to construct three regulatory networks to identify potential genes related to lung adenocarcinoma and squamous cell carcinoma and investigated the similarity and specificity of them. In the network, some of the observed transcription factors and target genes had been previously proven to be related to lung adenocarcinoma and squamous cell carcinoma. We also found some new transcription factors and target genes related to SCC. The results demonstrated that regulatory network analysis is useful in connection analysis between lung adenocarcinoma and squamous cell carcinoma.

Aldehyde Volatiles Emitted in Succession from Mechanically Damaged Leaves of Poplar Cuttings

( Zeng Hui Hu ),( Ying Bai Shen ),( You Qing Luo ),( Fan Yi Shen ),( Hai Bo Gao ),( Rong Fu Gao ) 한국식물학회 2008 Journal of Plant Biology Vol.51 No.4

Plant aldehydes are volatiles necessary to defenses against environmental stress. To explore their emissions in response to wounding, we performed gas chromatography-mass spectrometry (GC-MS) on cuttings from poplar (Populus simonii×P. pyramidalis ``Opera 8277``) that were mechanically damaged to mimic herbivore attack. We detected 16 aldehydes, including 11 linear saturated aldehydes, 3 linear unsaturated aldehydes, and 2 non-linear aldehydes. Emissions of these aldehydes were clearly enhanced by such treatment, and exhibited a similar pattern of change, i.e., increasing in the first 2 h, then sharply decreasing before rising again at about 12 h. Two release peaks for these aldehydes were observed. Therefore, we propose two pathways for the mediation of aldehyde emissions following damage. The first peak may represent emissions from plant storage pools, whereas the second release peak might result from greater formation de novo through an activated synthesis pathway.

Consumer attitudes toward downward extensions: an exploration of Giordano’s extension in Hong Kong

Fan Zeng,Stacy H. N. Lee,Chui Ming Heung 한국의류학회 2019 Fashion and Textiles Vol.6 No.1

Given that numerous fashion brands constantly struggle to survive, it is important to learn how consumers evaluate and change their attitudes toward the downward extensions of mass-production fashion businesses, particularly those that originate in Hong Kong. Through capitalizing on the parent brand’s equity earned through enhancing consumer preferences and building loyalty, companies’ brand extension strategies can lead to better profits and sales for fashion brands. Based on the categorization theory and the theory of perceived fit, this study examines how consumers’ familiarity, trust, perceptions of quality, and perceptions of the brand name of the parent brands influence them when evaluating a business’s downward extension. This study also investigates how the effect of the consistency, similarities in product features, and pricing of the extension’s brand concept influences consumers’ purchase intentions toward produces from the downward extension. To achieve the objectives of this study, snowball sampling was used to gather a total of 203 data sets for analysis through methods such as t-tests and stepwise regressions. The results indicated that familiarity with and trust in parent brands, along with the perceived quality of the parent brands’ products, were positive influences on consumers’ evaluations of downward extensions. The extensions’ consistency with the brand concept, product substitutability, product transferability, and pricing positively influenced higher purchase intentions. Consequently, this study highlights that perceptions of the quality of the parent brands contributes most strongly to positive evaluations, and the extension’s pricing contributes most significantly to purchase intentions.

Recovery of Native Protein from Potato Root Water by Expanded Bed Adsorption with Amberlite XAD7HP

Fan-Kui Zeng,Hong Liu,Peng-Jun Ma,Gang Liu 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.5

Potato root water (PRW) contains ~1.5%protein. In this study, expanded bed adsorption (EBA)chromatography with Amberlite XAD7HP resin adsorbent was used to isolate native protein from crude PRW. The optimal pH and ionic strength for potato protein binding onto Amberlite XAD7HP were 5.0 and 20 mmol/L. The EBA-refined proteins were dried by vacuum freeze drying and spray drying at varying outlet temperatures. Results indicated that low temperature spray drying was the most cost effective method with respect to retaining protease inhibitor activities. The dried protein concentrates appeared bright yellow or dark reddish brown, with a total glycoalkaloid content of ~170 μg/g. The protease inhibitor activity was ~400 mg/g and 11 ~ 12 mg/g for trypsin inhibition and chymotrypsin inhibition, respectively. The results presented here suggest that EBA using Amberlite XAD7HP as the adsorbent is a feasible strategy for the direct adsorption of native protein from crude PRW.