Development of a Conditional Replication Competent Adenovirus, Controlled by the Human Telomerase Promoter(hTERT)

Eunhee Kim,Joo-Hang Kim,Ha-Youn Shin,Han Saem Lee,Joo-Hyuk Sohn,양재명,김정호,Chae-Ok Yun 대한암학회 2003 Cancer Research and Treatment Vol.35 No.3

Purpose: This study has been planned to generate a replication-competent adenovirus which replicates in a cancer cell-specific manner, thus minimizing the side effects and toxicity of cancer gene therapy.Materials and Methods: we have generated an E1B 19 kD attenuated recombinant adenoviruses, Ad-TERT-19 and Ad-mTERT-19, which encode E1A gene driven by the wild type hTERT and modified m-hTERT promoter containing additional c-myc and Sp1 binding sites in the backbone of Ad-E1B19. The in vitro efficacy and specificity of the hTERT and m-hTERT promoter have been evaluated by the comparison of viral replication and cytopathic effect in cancer cells and normal cell lines. To assess anti-tumor effect and safety of hTERT or m-hTERT promoter driven replication competent adenoviruses, tumor regression after subcutaneous injection in subcutaneous C33A xenografts and lacZ expression after systemic injection in organs were examined.Results: The activation of hTERT or m-hTERT promoter was significantly up-regulated only in hTERT-positive cells, but not in hTERT-negative cells. Moreover, the activity of m-hTERT promoter was substantially increased in hTERT-positive cancer cells, but not in hTERT-negative cells. While Ad-TERT-19 replicated in and induced cytopathic effect in cancer and in some normal cell lines, Ad-mTERT-19 enhanced viral replication and cytopathic effect in cancer cells only. Furthermore, the growth of established human cervical carcinoma in nude mice was significantly suppressed by intratumoral injection of Ad-mTERT-19.Conclusions: The use of m-hTERT promoter is not only useful in the regulation of therapeutic gene expression but also that replication-competent oncolytic adenovirus under the control of m-hTERT promoter may be a new promising tool for the treatment of human malignancies.(Cancer Res Treat. 2003;35:191-206)

Increased Cytopathic Effect of Replicating Adenovirus Expressing Adenovirus Death Protein

Eunhee Kim,Joo-Hang Kim,구태영,Joo-Hyuk Sohn,Chae-Ok Yun 대한암학회 2003 Cancer Research and Treatment Vol.35 No.5

Purpose: Replication-competent adenoviruses (Ads)are promising new modalities for the treatment of cancer.Selective replication of a viral agent in tumor may leadto improved efficacy over non-replicating Ads due to viralmultiplication, lysis of the infected cancer cell and spreadto surrounding cells. In our previous studies it wasshown that the E1B 55 kD-deleted Ad (YKL-1) exhibitstumor specific replication and cell lysis, but with reducedcytolytic effects compared to the wild type adenovirus(Int J Cancer 2000;88:454-463). Thus, improving thepotency of oncolytic Ads remains an important goal forcancer gene therapy. To increase the oncolytic ability ofYKL-1, an adenovirus death protein (ADP) gene wasreintroduced under the control of a CMV or MLP promoterat the E3 region of the YKL-1, generating an YKL-cADPand YKL-mADP, respectively.Materials and Methods: The in vitro cytolytic effect ofADP expressing Ads was evaluated by MTT assay, andthe induction of apoptosis by ADP expressing Ads wasexamined by TUNEL analysis. Finally, the antitumor effectof ADP expressing Ads was demonstrated in C33A xenografttumor model.Results: The YKL-cADP exerted a markedly enhancedcytolytic effect against H460 and SK-Hep1 cancer celllines. The TUNEL assay indicated that the ADP-mediatedcytotoxicity was largely driven by apoptosis. Finally, theYKL-cADP showed a superior antitumor effect than theYKL-1 or YKL-mADP in C33A xenografts.Conclusion: These lines of evidence demonstrate thatthe YKL-cADP induces efficient cell lysis, which is criticalfor the addition of therapeutic value to replicating Ads incancer gene therapy. (Cancer Res Treat. 2003;35:425-432)

소마토스타틴 수용체

김은희,손숙진,이민아,박희순,정지창,박승준 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.4

Personality Types as Predictors of Breast Cancer Screening Compliance in Korean Patients: A Mixed-Method Approach

Kyungmin Kim,Eunhee Sohn,Hyo-Deog Rim,Seoyoung Jang,Jungmin Woo 대한신경정신의학회 2020 PSYCHIATRY INVESTIGATION Vol.17 No.11

Objective The purpose of this study is to identify personality types that can influence breast cancer screening (BCS) compliance among Korean women with breast cancer using a mixed-method approach. Methods The participants consisted of 93 women who underwent surgery for breast cancer between July 2010 and March 2012. The demographic and medical characteristics of the participants were evaluated through structured interviews. To identify personality types, in-depth interviews were performed and the transcribed interviews were evaluated using interpretive phenomenological analysis. The participants were categorized into two groups (compliance and non-compliance) based on compliance with the Korean Breast Cancer Society recommendations for BCS. Results Five personality types were identified through phenomenological analysis. There were significant differences in the chi-square test results for the BCS compliance and non-compliance groups according to age (p=0.048), cancer stage (p<0.001), and personality types (p=0.018). Logistic regression showed that the odds ratio for compliance with BCS was 9.35 (p=0.01) for individuals with a cautious- organized personality type, 9.38 (p=0.02) for those with a cautious-dependent personality, and 10.58 (p=0.04) for those with a sensitive-downcast personality compared to those with a cautious personality type. Conclusion Participants with cautious-organized, cautious-dependent, and sensitive-downcast personality types were less likely to follow the BCS recommendations than those with a cautious personality type. This study provides a basis for the future development of an effective questionnaire to investigate the personality types of individuals with breast cancer in order to predict compliance with BCS.

Repetitive transcranial magnetic stimulation for neuropathic pain

Kim, Sooyoung,Lee, Eun Kyoung,Sohn, Eunhee The Korean Society of Clinical Neurophysiology 2022 Annals of Clinical Neurophysiology Vol.24 No.2

Noninvasive stimulation of the nervous system for treating chronic neuropathic pain has received attention because of its tolerability and relative efficacy. Repetitive transcranial magnetic stimulation (rTMS) is a representative method of noninvasive brain stimulation. Evidence-based guidelines on therapeutic use of rTMS have been proposed recently for several neurological diseases. These guidelines recommend treating neuropathic pain by applying high-frequency (≥ 5 Hz) rTMS to the primary motor cortex contralateral to the painful side. This review summarizes the mechanisms and guidelines of rTMS for treating neuropathic pain, and proposes directions for future research.

Subgrouping of Peripheral Neuropathic Pain Patients According to Sensory Symptom Profile Using the Korean Version of the PainDETECT Questionnaire

Kyomin Choi,Ohyun Kwon,Bum Chun Suh,Eunhee Sohn,In Soo Joo,오지영 대한의학회 2022 Journal of Korean medical science Vol.37 No.3

Background: A culturally validated Korean version of the PainDETECT Questionnaire (PD-Q) was used to identify neuropathic pain components (NeP) in patients suffering from chronic pain. The purpose of this study was to determine if the Korean PD-Q can be used to subgroup patients with peripheral NeP according to sensory symptom profiles. Methods: This study included 400 Korean patients with peripheral neuropathic pain diagnosed as probable or definite NeP. The total scores and subscores for each item in PD-Q were transformed into a Z-score for standardization. Hierarchical cluster analysis was performed to identify clusters of subjects by PD-Q scores. Results: The mean total PD-Q score of the study participants was 14.57 ± 6.46. A hierarchical cluster analysis identified 5 clusters with distinct pain characteristic profiles. Cluster 1 had relatively severe burning and tingling sensations. The mean total PD-Q score for cluster 2 was the lowest of the 5 clusters. Cluster 3 tended to be vulnerable to pain in response to cold/heat stimulation. Cluster 4 showed relatively severe pain induced by physical stimuli, such as light touch or slight pressure. Cluster 5 had high scores for all NeP symptoms. Conclusion: This study demonstrates the ability of patients to cluster by symptoms using the Korean PD-Q. Subgrouping of peripheral neuropathic pain by sensory symptom profile may be useful in making effective drug treatment decisions.

초수평선 레이다를 위한 고해상도 거리분해능 기술에 관한 연구

김형수(Hyoung-soo Kim),손성환(Sunghwan Sohn),문현욱(Hyunwook Moon),이기원(Kiwon Lee),최준혁(Jun Hyeok Choi),김은희(Eunhee Kim) 한국전자파학회 2024 한국전자파학회논문지 Vol.35 No.1

초수평선 레이다(over-the-horizon radar, OTH radar)는 지구 곡률을 따라 레이다 신호를 지평선 너머로 전파시켜 멀리 떨어진 다양한 대상을 탐지하고 추적하는 기술이다. 이 기술은 이웃 국가로부터의 잠재적인 위협에 대응하기 위해 중요한 역할을 하지만, 아직까지 국내에서는 구현되어 실전 사용이 되지 못하고 있다. 초수평선 레이다 시스템은 멀리 떨어진 대상을 감지하기 위해 단파 주파수(3∼30 MHz)를 사용하는데, 이로 인해 대역폭이 제한되어 결과적으로 거리 해상도가 낮아지는 단점이 있다. 이러한 한계를 극복하기 위해 본 연구는 좁은 대역폭의 전송 펄스 파형의 거리 해상도를 향상시키기 위해 하이브리드 최소자승법 수신 필터를 구성하는 데 초점을 맞추었다. 이 필터는 기존의 정합 필터와 최소자승법을 결합하여 높은 해상도를 유지하면서도 노이즈를 효과적으로 제어한다. 마지막으로, 시뮬레이션 결과들로부터, 이 하이브리드 최소자승법의 성능을 검증하였다. Over-the-horizon (OTH) radar is a technology that propagates radar signals beyond the Earths curvature along the horizon to detect and track a wide range of distant targets. This technology is crucial in addressing potential threats from neighboring countries; however, it has not been implemented domestically for practical use. The OTH radar system utilizes high frequencies (3∼30 MHz) to detect distant targets, resulting in a limited bandwidth and, consequently, reduced range resolution. To overcome these limitations, this study focused on enhancing the range resolution of narrow-bandwidth transmission pulse waveforms by constructing a hybrid least-squares reception filter. This filter combines the outputs processed using matched filters and the least-squares method to effectively maintain a high resolution while controlling noise. Finally, simulation results confirmed the improvement in the range resolution of the OTH radar through the application of this hybrid reception filter.

Tumor-mimicking primary angiitis of the central nervous system: initial and follow-up MR features

Lee, Youkyung,Kim, Ji-hoon,Kim, Eunhee,Park, Sung-Hye,Yim, Yoo Jeong,Sohn, Chul-Ho,Chang, Kee-Hyun Springer-Verlag 2009 Neuroradiology Vol.51 No.10

다지역 임상시험의 계획 및 설계에 대한 국제 제도적 동향 분석

송윤경,손민지,전아영,김재현,지은희,오정미,김인화,Song, Yun-Kyoung,Sohn, Minji,Jeon, Ah Young,Kim, Jae Hyun,Ji, Eunhee,Oh, Jung Mi,Kim, In-Wha 한국임상약학회 2018 한국임상약학회지 Vol.28 No.2

Objective: Multi-regional clinical trials have been widely used for accelerating global drug development by multinational pharmaceutical companies. In this study, we aimed to review and analyze the international trends in regulations and guidelines on multi-regional clinical trials by regulatory authorities and international organizations, such as International Conference on Harmonisation, for referring to policies, including development of domestic guidelines for multi-regional clinical trials. Methods: The policies, regulations, and guidelines published by the US Food and Drug Administration, European Medicines Agency, Pharmaceuticals and Medical Devices Agency (Japan), and China Food and Drug Administration were searched, and the International Conference on Harmonisation E17 draft guideline was reviewed. Results: The regulatory authorities in developed countries have developed and implemented regulations and guidelines on multi-regional clinical trials to promote simultaneous global drug development and evaluate the regional differences in drug safety and efficacy. International Conference on Harmonisation developed the draft guideline for planning/designing of multi-regional clinical trials in 2016, which recommends the general principles for strategy-related issues and design of multi-regional clinical trials, and for protocol-related issues, such as consideration of regional variability, subject selection, dose selection, endpoints, comparators, overall sample size, allocation to regions, collecting information on efficacy and safety, and statistical analysis. Conclusion: It is important to understand the international regulatory requirements for designing and planning of multi-regional clinical trials for global drug development. Moreover, it is necessary to prepare multi-regional clinical trial guidelines in accordance with the Korean regulation for clinical trials and drug administration.

Characteristics of Diverse Verbal Pain Descriptors in South Korean Patients With Peripheral Neuropathic Pain: ‘Jeorim’ (Tingling) and ‘Sirim’ (Cold) as Key Neuropathic Pain Descriptors

Kyomin Choi,Ohyun Kwon,Bum Chun Suh,Jeeyoung Oh,Sungkun Cho,Eunhee Sohn,In Soo Joo 대한신경과학회 2023 Journal of Clinical Neurology Vol.19 No.3

Background and Purpose The description of pain is the most-important indicator leading to the adequate treatment of patients with neuropathic pain (NeP). The purpose of this study was to identify and characterize the unique features of Korean verbal descriptions in patients with peripheral NeP. Methods This study included 400 patients (167 males and 233 females) and their 1,387 paindescription responses. Patients with peripheral NeP freely described their symptoms in Korean. Collected verbal descriptions were grouped according to terminologies with similar meanings. Participants completed validated patient-reported outcome scales including the neuropathic pain symptom inventory (NPSI) and painDETECT questionnaire (PD-Q). The frequencies of each verbal pain descriptor were compared between the NPSI and PD-Q scores. Results ‘Jeorim’ (tingling) was the most common among 17 types of organized verbal pain descriptors, and the ‘Sirim’ (cold) symptom had a significantly higher rate of use in the 2 highseverity groups when participants were classified by their total scores on the NPSI and PD-Q. Conclusions Korean verbal NeP descriptors were significantly diverse. The Jeorim (tingling) and Sirim (cold) descriptors can be utilized in evaluations of Korean patients with NeP.