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이은덕,심재형,안규환,백인환 한국공작기계학회 2003 한국생산제조학회지 Vol.12 No.1
Keeping the layer thickness constant is very essential for improving the shape accuracy in the stereolithography process. The layer thickness is created by recoating process, and also affected by recoating parameters such as blade speed and thickness. The created layer in this process can determine the whole accuracy of the entire parts. The aim of this paper is to improve the accuracy of the layer thickness by adjusting the recoating process parameters. Several experiments with different recoating conditions are performed to find the optimal recoating parameters that produce the most accurate layer thickness. The effective recoating method is suggested by measuring and analyzing the cured layer thickness.
Determination of Process Parameters in Stereo lithography Using Neural Network
Lee, Eun-Dok,Sim, Jae-Hyung,Kweon, Hyeog-Jun,Paik, In-Hwan The Korean Society of Mechanical Engineers 2004 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.18 No.3
For stereo lithography process, accuracy of prototypes is related to laser power, scan speed, scan width, scan pattern, layer thickness, resin characteristics and etc. An accurate prototype is obtained by using appropriate process parameters. In order to determine these parameters, the stereolithography (SLA) machine using neural network was developed and efficiency of the developed SLA machine was compared with that of the traditional SLA. Optimum values for scan speed, hatching spacing and layer thickness improved the surface roughness and build time for the developed SLA.
Determination of Process Parameters in Stereolithography Using Neural Network
Eun-Dok Lee,Jae-Hyung Sim,Hyeog-Jun Kweon,In-Hwan Paik 대한기계학회 2004 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.18 No.3
For stereolithography process, accuracy of prototypes is related to laser power, scan speed, scan width, scan pattern, layer thickness, resin characteristics and etc. An accurate prototype is obtained by using appropriate process parameters. In order to determine these parameters, the stereolithography (SLA) machine using neural network was developed and efficiency of the developed SLA machine was compared with that of the traditional SLA. Optimum values for scan speed, hatching spacing and layer thickness improved the surface roughness and build time for the developed SLA.<br/>
Gil, Minchan,Pak, Hyo-Kyung,Park, Seo-Jeong,Lee, A-Neum,Park, Young-Soo,Lee, Hyangsin,Lee, Hyunji,Kim, Kyung-Eun,Lee, Kyung Jin,Yoon, Dok Hyun,Chung, Yoo-Sam,Park, Chan-Sik 한국조명·전기설비학회 2015 한국조명·전기설비학회 학술대회논문집 Vol. No.
<P>CD99 signaling is crucial to a diverse range of biological functions including survival and proliferation. CD99 engagement is reported to augment activator protein-1 (AP-1) activity through mitogen-activated protein (MAP) kinase pathways in a T-lymphoblastic lymphoma cell line Jurkat and in breast cancer cell lines. In this study, we report that CD99 differentially regulated AP-1 activity in the human myeloma cell line RPMI8226. CD99 was highly expressed and the CD99 engagement led to activation of the MAP kinases, but suppressed AP-1 activity by inducing the expression of basic leucine zipper transcription factor, ATF-like (BATF), a negative regulator of AP-1 in RPMI8226 cells. By contrast, engagement of CD99 enhanced AP-1 activity and did not change the BATF expression in Jurkat cells. CD99 engagement reduced the proliferation of RPMI8226 cells and expression of cyclin 1 and 3. Overall, these results suggest novel CD99 functions in RPMI8226 cells.</P>
Lee, Kyo Ree,Kang, Eun Sung,Kim, Yong-Tae,Kim, Nam Hyeong,Youn, David,Kim, Young Dok,Lee, Jaeyoung,Kim, Yong Ho Elsevier Science Publishers 2017 Catalysis today Vol.295 No.-
<P><B>Abstract</B></P> <P>Improving the efficiency of the oxygen reduction reaction (ORR) for use in commercial fuel cells has been the subject of diverse synthetic research activities. However, due to their inferior catalytic performance and dispensability, there are still limitations to achieving breakthroughs regarding ORR catalytic activity using carbon nanomaterials, despite their high electron-transfer and mass-transport properties. In this study, we mimicked nature by using a biomineralization approach for controlling the growth of inorganic materials and demonstrated improved ORR values. The designed peptide, Hexcoil-Ala, is able to supramolecular assembly on single-walled carbon nanotubes (SWNTs), leading to SWNTs that are well dispersed in aqueous solution. In order to direct gold nanoparticle (AuNP) nucleation sites, we substitute two residues in Hexcoil-Ala with cysteine to provide the mutated peptide, HexCoil-Ala-2Cys. This peptide affords a sophisticated, size-controlled, and well-dispersed arrangement of AuNPs. High-resolution transmission electron microscopy studies confirmed the homogeneously well-aligned distribution of nanosized AuNPs on the HexCoil-Ala-2Cys structure, along the direction of SWNT axis. The (AuNPs/P-SWNT) composite in water provides dispersed and stable metallic nanoparticles of electrostatically modified Au through synergistic effects involving the peptide. Consequently, this catalyst exhibits improved ORR performance compared to bulk gold and even, in case of number of electrons (<I>n</I>) transferred, higher than the number of Pt/C. X-ray photoelectron and Raman spectroscopies reveal the details of the electronic interactions among the components of the AuNP/P-SWNT composite, and how they facilitate the four-electron reaction pathway. This study provides valuable information for the optimization of catalyst synthesis and precise particle-size control, leading to stable, water-dispersive composites, with improved electronic properties for enhanced ORR performance in fuel cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We used designed peptide to direct nucleation sites of gold nanoparticles. </LI> <LI> The designed peptide offers size-controlled and well-dispersed arrangement of AuNPs. </LI> <LI> Also, the superstructure of AuNPs was modified electrostatistically by the peptide. </LI> <LI> It exhibits improved ORR performance with higher number of electrons(<I>n</I>) transferred. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>In this present work, we describe improved electrocatalytic reaction of directly gold nanoparticle assembled superstructures using the programmed supramolecular protein assembly.</P> <P>[DISPLAY OMISSION]</P>
CTX Prophages in Vibrio cholerae O1 Strains
( Eun Jin Kim ),( Dok Yung Lee ),( Se Hoon Moon ),( Chan Hee Lee ),( Dong Wook Kim ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.6
The classical biotype strains of the Vibrio cholerae O1 serogroup harbor the biotype-specific cholera-toxin encoding phage (CTX) CTXcla, and the El Tor biotype strains contain CTX-1. Although the classical biotype strains have become extinct, a remnant of classical CTX phage is transferred to the El Tor biotype strains. The prototype El Tor strains, which produce the biotype-specific cholera toxin, are now being replaced by atypical El Tor variant strains producing classical biotype cholera toxin. The genome sequences of the CTX phages in atypical El Tor strains indicate that the CTX phages in atypical El Tor strains are a mosaic of CTXcla and CTX-1. Before the emergence of atypical El Tor stains in the early 1990s, unusual pre-seventh pandemic strains were isolated in the US Gulf Coast between 1973 and 1986. These strains have characteristics of atypical El Tor strains since they are El Tor biotype strains containing CTXcla, yet the genome sequence of this CTX phage indicates that it is different from CTXcla and is therefore classified separately as CTXUS Gulf.