자가 면역성 간염에서 자가항체의 발현 양상과 스테로이드 치료의 효과

박기오,채경훈,허원석,강윤세,정재훈,김연수,문희석,이엄석,김선문,이병석,김남재,이헌영 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

목적 : 최근 8년간 본원에서 경험한 자기면역성 간염의 혈액학적 특징과 스테로이드의 치료효과를 알아보고자 본 연구를 시행하였다. 방법 : 1996년 이후부터 2002년까지 충남대학교 병원에서 1999년 International Autoimmune Hepatitis Group(LAHG)에서 제시한 수정 진단 기준안에 의거하여 총 진단점수가 probable 이상에 해당하는 환자들을 대상으로 임상적 특징, 검사실 및 조직 소견과 스테로이드와 azathioprine의 치료 효과를 후향적으로 조사하였다. 결과 : 9예의 환자들은 여성이 78%, 40대가 많았고, 급성 발병이 33.3%, 피로감, 식욕부진 및 황달 등이 나타났으며, 항핵항체가 55.6%에서 그리고 항평활근 항체가 77.8%에서 양성이었고, 동반 질환은 류마치스 관절염이 2예, 특발성 혈소판 감소증 1예, Henoch-Sch nlein 자반증 및 그레브스병이 각각 1예 씩이었다. 7예에 대해서 스테로이드와 azathioprine으로 치료하여 생화학적 임상적 완해가 이루어져서 유효한 치료 결과를 얻었다. 결론 : 자가면역성 간염은 우리 나라에서 원인을 찾을 수 없는 만성 간질환의 경우, 특히 중년여성에서 다른 자가면역성 질환이 동반되었을 때 자가면역성 간염을 의심하여 자가항체 선별검사를 시행해 보아야 할 것으로 생각된다. 향후 다기관 연구를 통하여 좀 더 많은 증례를 모으고 전향적인 연구를 시행하여야 할 필요가 있다. Although autoimmune hepatitis(AIH) is common in western contries, some studties have been reported in Korea. The aim of the study was to analyze the clinical and histological features and expression pattern of the autoantibody and to examine clinical course of AIH in Korea. For 9 patients diagnosed as having AIH in our hospital since 1996, we reviewed medical records and analyzed clinical and laboratory findings retrospectively. They had a probable or definite AIH according to the revised criteria of International AIH group. The mean age was 42.7(21∼68)years and 7(77.8%) were female. Three of them(33.3%) showed acute hepatitis and five of them showed cirrhosis. Fatigue and anorexia were the most frequently complained symptoms(55.6%). Four patients(44.4%) were accompanied with other autoimmune diseases. Antinuclear antibody was detected in 5(55.6%) and anti-smooth muscle antibody was detected in 7(77.8%). Three patients showed focal picemeal necrosis and four showed periportal fibrosis. Among 7 patients who received immunosuppressive therapy, six patients(85.7%) showed initial response. and biochemical and serologic remission were achived in 5(71.4%) and 3(42.8%) patients, AIH seems to be partly responsible for chronic liver disease of unknown etiology in Korea. AIH should be suspected in female patients with unknown chronic liver disease of unknown etiolgy, especially accompanied with other autoimmune disease. The clinical characteristics of AIH may not be quite different from those of Western countries.

간세포암과 감별이 어려웠던 악성중피종 치험 1예

김선문,허원석,채경훈,강윤세,정재훈,김연수,박기오,문희석,이엄석,김석현,성재규,이병석,이헌영,신경숙,조준식,송인상,강대영 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Malignant peritoneal mesothelioma is a rare neoplasm that arises from the mesothelium of a serosal cavity and is a rapidly fatal disease with a median survival of 4 to 12 months for untreated cases. Recently, we experienced a case with malignant peritoneal mesothelioma who was suspected hepatocelluar carcioma by abdominal CT scan and was confirmed by biopsy including immunohistochemical stain(calretinin) after surgery. We performed tumor excisions and wedge resection of the liver(segment Ⅷ)and inserted Tencoff catheter in abdominal cavity at 25th day of post-operation. We treated with intraperitoneal paclitaxel(25mg/m^(2)/day for 5 days) six courses monthly. She was well tolerable and is still living without any evidence of recurrence for 14th month of post-operation.

The Interaction between Epidermal Growth Factor (EGF) and Follicular Stimulating Hormone (FSH) on Nuclear Maturation of Mouse Oocytes by Using Their Inhibitor

Cha, Soo-Kyung,Kim, Tae-Hyung,Eum, Jin-Hee,Park, Kang-Hee,Park, Eun-A,Kim, Seung-Bum,Chung, Mi-Kyung,Lee, Dong-Ryul,Ko, Jung-Jae 한국수정란이식학회 2002 한국수정란이식학회 학술대회 Vol.2002 No.1

The stimulatory effect of EGF and FSH on oocyte maturation have been reported in various mammalian species. And some reports presented FSH enhanced the effect of EGF on oocyte maturation. But, the interaction between EGF and FSH on nuclear maturation of mammalian oocytes is not fully understood. We observed the effect of EGF and FSH on nuclear maturation during in vitro maturation of mouse oocytes. Also, we examined the interaction between EGF and FSH on nuclear maturation of mouse oocytes using the EGFR inhibitor or FSH inhibitor. Germinal vesicle (GV) stage oocytes were obtained from 3-4weeks PMSG primed BCFI hybrid mice and cultured in TCM-199 medium with 0.4%PVP supplemented with/without EGF (1ng/ml), FSH (1ug/ml), EGFR specific tyrosine kinase inhibitors: Tyrphostin AG 1478 (500nM), MAP kinase kinase inhibitor : U0126 (20uM) or PD 98059 (100uM) for 14-l5hr. Rapid staining method were used for the assessment of nuclear maturation. Nuclear maturation rates of EGF indjor FSH-treated group were significantly higher than those of control group. Treatment of EGFR inhibitor significantly block the nuclear maturation of GV oocyte in EGF-treated group, but it did not block those of GV oocyte in FSH-treated or FSH and EGF-treated group. Treatment of FSH inhibitor(U0126, PD98059) significantly block the nuclear maturation of EGF-treated group, FSH-treated and FSH and EGF-treated group. These results show that EGF has a stimulatory effect as well as different action pathway with FSH on in-vitro maturation of mouse oocyte in vitro. Therefore, further studies will be needed to find the signaling pathway of EGF associated with nuclear maturation.

인두구와 쉰 목소리 환자의 위식도 및 위후두 역류 빈도

박찬원 ( Chan Won Park ),최철웅 ( Cheol Woong Choi ),김광하 ( Gwang Ha Kim ),김경엽 ( Kyung Yeob Kim ),하태인 ( Tae In Ha ),박형열 ( Hyoung Yoel Park ),엄재섭 ( Jae Sup Eum ),박찬호 ( Chan Ho Park ),이선미 ( Sun Mi Lee ),강대환 ( 대한소화기기능성질환·운동학회 2008 Journal of Neurogastroenterology and Motility (JNM Vol.14 No.1

목적 : 인두구 및 쉰 목소리 같은 만성 인후두 증상이 위식도 역류 질환 및 식도 운동성 질환과 관련이 있다고 알려져 있다. 인두구와 쉰 목소리를 호소하는 환자에서 위식도 역류 및 위인두 역류와의 관계를 후향적으로 조사하고자 하였다. 대상 및 방법 : 인두구와 쉰 목소리를 호소하는 290명의 환자(남자 122명, 여자 168명)를 대상으로 하여, 위내시경검사, 24시간 보행성 식도산도검사 및 식도내압검사를 시행하였다. 내시경 소견, 24시간 보행성 식도산도검사 및 식도 내압 검사 소견을 분석하였다. 결과 : 1) 위산 역류의 전형적인 증상은 51.7% (150/290)에서 관찰되었다. 2) 내시경 검사에서 역류성 식도염은 12.2% (29/236), 식도열공탈장은 5.9% (14/236)에서 관찰되었다. 3) 식도내압 검사에서 비정상 소견을 보이는 경우는 64.8%였으며, 이중 비효과적 식도운동이 가장 많았다. 4) 24시간 보행성 식도산도검사에서 정상 소견은 34.2% (91/266), 위식도역류질 환으로만 진단된 경우는 14.3% (38/266), 위후두역류질환으로만 진단된 경우는 15.0% (40/266), 위식도역류질환과 위후두역류질환 모두로 진단된 경우는 36.5% (97/266)이었다. 결론 : 인두구와 쉰 목소리를 호소하는 환자에서 위식도 역류질환 및 위후두 역류질환의 빈도가 높게 나타났다. 그러므로, 후두경 검사상 정상인 인두구 및 쉰 목소리를 호소하는 환자에서 위식도 역류 및 식도운동질환에 대한 검사를 시행하고 이에 따라 치료적 접근을 하는 것이 바람직 하다고 사료된다. Background/Aims: Globus pharyngeus and hoarseness are common extraesophageal manifestations in the patients with astroesophageal reflux disease. The aim of this study was to evaluate the frequency of gastroesophageal and gastropharyngeal reflux in the patients with globus and/or hoarseness. Methods: From July 2004 to December 2006, we retrospectively analyzed the medical records and the findings from endoscopy, esophageal manometry and ambulatory 24-hour pH monitoring of patients with symptoms of globuspharyngeus or hoarseness. Results: One hundred fifty patients (51.7%, 150/290) complained typical reflux symptoms such as heartburn or acid regurgitation. Two hundred thirty-six patients underwent endoscopy. Reflux esophagitis and hiatal hernia were found in 12.2% (29/236) cases and 5.9% (14/236) cases, respectively. Abnormal esophageal motilities were found on 64.8% of patients showed abnormal esophageal motilitiy tests. Ineffective esophageal motility was the most frequent finding. Two hundred sixty-six patients underwent ambulatory 24-hour dual probe pH monitoring. Of them, normal PH monitoring , GERD only, GPRD only and both GERD and GPRD were 34.2% (91/266), 14.3% (38/266), 15.0% (40/266) and 36.5% (97/266), respectively. Conclusions: In a high proportion of the patients with globus pharygeus and hoarseness, gastroesophageal reflux disease and gastropharyngeal reflux disease were observed.

Clinical outcomes of single versus double blastocyst transfer in fresh and vitrified-warmed cycles

Eum, Jin Hee,Park, Jae Kyun,Kim, So Young,Paek, Soo Kyung,Seok, Hyun Ha,Chang, Eun Mi,Lee, Dong Ryul,Lee, Woo Sik The Korean Society for Reproductive Medicine 2016 Clinical and Experimental Reproductive Medicine Vol.43 No.3

Objective: Assisted reproductive technology has been associated with an increase in multiple pregnancies. The most effective strategy for reducing multiple pregnancies is single embryo transfer. Beginning in October 2015, the National Supporting Program for Infertility in South Korea has limited the number of embryos that can be transferred per in vitro fertilization (IVF) cycle depending on the patient's age. However, little is known regarding the effect of age and number of transferred embryos on the clinical outcomes of Korean patients. Thus, this study was performed to evaluate the effect of the number of transferred blastocysts on clinical outcomes. Methods: This study was carried out in the Fertility Center of CHA Gangnam Medical Center from January 2013 to December 2014. The clinical outcomes of 514 women who underwent the transfer of one or two blastocysts on day 5 after IVF and of 721 women who underwent the transfer of one or two vitrified-warmed blastocysts were analyzed retrospectively. Results: For both fresh and vitrified-warmed cycles, the clinical pregnancy rate and live birth or ongoing pregnancy rate were not significantly different between patients who underwent elective single blastocyst transfer (eSBT) and patients who underwent double blastocyst transfer (DBT), regardless of age. However, the multiple pregnancy rate was significantly lower in the eSBT group than in the DBT group. Conclusion: The clinical outcomes of eSBT and DBT were equivalent, but eSBT had a lower risk of multiple pregnancy and is, therefore, the best option.

Ficus vasculosa Wall. ex Miq. Inhibits the LPS-Induced Inflammation in RAW264.7 Macrophages

Park JI-Won,Park Jin-Mi,Eum Sangmi,Kim Jung Hee,Oh Jae Hoon,Choi Jinseon,Bach Tran The,Sinh Nguyen Van,Choi Sangho,Ahn Kyung-Seop,Lee Jae-Won 한국미생물·생명공학회 2022 한국미생물·생명공학회지 Vol.50 No.4

Ficus vasculosa Wall. ex Miq. (FV) has been used as a herbal medicine in Southeast Asia and its antioxidant activity has been shown in previous studies. However, it has not yet been elucidated whether FV exerts anti-inflammatory effects on activated-macrophages. Thus, we aimed to evaluate the ameliorative property of FV methanol extract (FM) on lipopolysaccharide (LPS)-induced inflammatory responses and the underlying molecular mechanisms in RAW264.7 macrophages. The experimental results indicated that FM decreased the production of inflammatory mediators (NO/PGE2) and the mRNA/protein expression of iNOS and COX-2 in LPS-stimulated RAW264.7 cells. FM also reduced the secretion of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and monocyte chemoattractant protein (MCP)-1 in LPS-stimulated RAW264.7 cells. Results also demonstrated that FM improved inflammatory response in LPS-stimulated A549 airway epithelial cells by inhibiting the production of cytokines, such as IL-1β, IL-6 and TNF-α. In addition, FM suppressed MAPK activation and NF-κB nuclear translocation induced by LPS. FM also upregulated the mRNA/protein expression levels of heme oxygenase-1 and the nuclear translocation of nuclear factor erythroid 2-related factor 2 in RAW264.7 cells. In an experimental animal model of LPSinduced acute lung injury, the increased levels of molecules in bronchoalveolar lavage (BAL) fluid were suppressed by FM administration. Collectively, it was founded that FM has anti-inflammatory properties on activated-macrophages by suppressing inflammatory molecules and regulating the activation of MAPK/ NF-κB signaling.

Dipterocarpus obtusifolius attenuates the effects of lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages

Park, Ji-Won,Kwon, Ok-Kyoung,Oh, Sei-Ryang,Lee, Joongku,Eum, Sangmi,Nguon, Samnang,Choi, Sang Ho,Khiev, Piseth,Ahn, Kyung-Seop SPANDIDOS PUBLICATIONS 2017 MOLECULAR MEDICINE REPORTS Vol.16 No.6

<P>Dipterocarpus obtusifolius has been traditionally used as a herbal medicine and is considered to have anticancer properties. The biological activity of D. obtusifolius in inflammation and the underlying mechanisms of its activity remain to be elucidated. The present study investigated the effects of D. obtusifolius methanolic extract (DOME) on lipopolysaccharide (LPS)-stimulated inflammation in RAW264.7 cells. The effects of DOME on the production of nitric oxide, prostaglandin E-2 and pro-inflammatory cytokines were assessed by ELISA, western blot analysis and reverse transcription-quantitative polymerase chain reaction. It was demonstrated that expression of inducible nitric oxide synthase, cyclooxygenase-2, interleukin-1 beta and tumor necrosis factor-a was suppressed by DOME in LPS-stimulated cells. Furthermore, treatment with DOME suppressed phosphorylation of mitogen activated protein kinase (MAPK) molecules, including extracellular signal-regulated kinase, c-Jun N-terminal kinase and p38 MAPK. Translocation of the nuclear factor-kappa B p65 subunit into the nucleus was additionally inhibited by DOME. Phosphorylation of MAPK promoter activity was inhibited by treatment with DOME, PD98059, SB202190 and SP600125. These results demonstrated that DOME inhibits LPS-induced inflammatory responses. Therefore, DOME may be a potential therapeutic approach for the treatment of inflammatory diseases.</P>

A Novel Antimicrobial Molecule from Medicinal Insect

Leem Jae-Yoon,Lee Hyun-woo,Park Ho-Yong,park Doo-Sang,Kim Mi-Seong,Ko Mi-Kyung,Eum Jung-Seon 대한약학회 2003 大韓藥學會 總會 및 學術大會 Vol.2003 No.2

초등 체육에서의 다중지능과 Kagan 구조의 탐색

박경준 ( Kyung Jun Park ),엄혁주 ( Hyok Ju Eum ) 고려대학교 스포츠과학연구소 2010 스포츠科學論叢 Vol.16 No.-

본 연구는 초등학교 체육 교육의 맥락에서 다중지능 이론과 S. Kagan의 협동학습에 관한 적용가능성 알아보고자 한다. 협동학습은 긍정적인 인간관계, 동료간의 협력, 활발한 학습, 학업 성취, 동등한 참여, 그리고 교실에서의 학생간의 동등한 역할을 강화하기 위한 구조화된 동료 상호작용의 한 형태이다. 이에 대해 Kagan은 수업적 구조(Instructonal Structures)로서 협동학습을 구조중심으로 접근하는 방법인 Kagan 구조를 개발하였다. 특히, 특정한 Kagan 구조는 학습에 참여를 통한 학생 개개인의 특성과 개성을 발휘할 수 있는 기회를 골고루 제공함으로써 학생들의 다중 지능을 학습하는 데에도 효과적이다. 그러므로 이 연구는 Kagan 구조의 의미와 기본 원리를 알아보고 다중지능 이론과 Kagan 구조의 관계를 이해함으로써 체육교육에 실제 적용할 수 있는 방법에 대해 논의하고자 한다. 이는 체육수업에서의 학생들의 긍정적인 수업풍토와 참여의 극대화 그리고 교육의 효과를 높이는데 많은 시사점을 제공할 것으로 기대된다. In this article, I would like to make Inquiry a little further applying based on Multiple intelligences theory and Spencer Kagan`s Structures of the physical education in the elementary school. Cooperative Learning is a pattern of structured peer interaction emphasizing collaboration between peers, academic achievement, active learning, positive human relationships, equal participation, and equal status of students in the school. Kagan developed Kagan`s approach to cooperative learning. All intelligences can be developed by Any particular structure. And students learn best when at least part of the time they have participate to the subject learning through their preferred intelligence or intelligences. Thus, This paper discusses concepts of the Kagan`s structures proposed by Spencer Kagan, Multiple intelligences and Kagan`s structura1 approach and mathod of applying in physical learning and activities. In consequence, This study was expected to provide a positive class setting between students, highly students paticipation, and incleasing the educational effect.

호남고속철도 테스트베드 구간 시설물 계측관리를 위한 통합상황실 구축에 관한 연구

박영곤 ( Park Young Kon ),엄기영 ( Eum Ki Young ),윤희택 ( Yoon Hee Taek ),황경훈 ( Hwang Kyung Hun ) 한국구조물진단유지관리공학회 2014 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.18 No.2