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Cho, Jungheum,Lee, Joon Woo,Lee, Eugene,Kang, Yusuhn,Cho, Ha Ra,Kim, Dong Yoon,Ho, Myoung Jin,Kang, Myung Joo,Choi, Yong Seok The Korean Pain Society 2019 The Korean Journal of Pain Vol.32 No.4
Background: To develop a rabbit epidural steroid injection (ESI) model for analyzing steroid retention in the tissue, and to assess the difference in steroid retention in the model according to the location and time elapsed after ESI. Methods: Fluoroscopy-guided ESI was performed using the interlaminar approach between the lowest two lumbar segments in 13 female New Zealand white rabbits. Four rabbits were allocated to each of three different groups according to the time of sacrifice: 3, 7, and 15 days post-ESI; the remaining rabbit was sacrificed immediately post-ESI to obtain baseline data. After sacrifice, two segments were harvested: the lowest two lumbar vertebrae and another two lumbar vertebrae immediately above these. The residual steroid amount (RSA) and residual steroid concentration (RSC) in the collected spinal columns were analyzed. A linear mixed model was used to compare RSAs and RSCs between the injected and adjacent segments, and among the number of days until sacrifice; P < 0.05 was considered statistically significant. Results: Both RSA and RSC of the injected segment were significantly higher than those of the adjacent segment (P < 0.001, both). The RSA and RSC significantly decreased over time (P = 0.009 and P = 0.016, respectively). Conclusions: The developed rabbit ESI model verified that significantly more steroid was retained at the injected segment than at the adjacent segment and the residual steroid decreased over time. This model could be useful not only for comparing current steroid medications, but also for developing new, better steroid formulations.
Peptoniphilus mikwangii sp. nov., Isolated from a Clinical Specimen of Human Origin
Cho, Eugene,Park, Soon-Nang,Shin, Yeseul,Lim, Yun Kyong,Paek, Jayoung,Kim, Hak Kyun,Hwang, Cheol Ho,Jo, Eojin,Jin, Dongchun,Chang, Young-Hyo,Kook, Joong-Ki Springer-Verlag 2015 Current microbiology Vol.70 No.2
<P>A novel Gram-positive, anaerobic, and coccus-shaped bacterium, designated as strain ChDC B134(T), was isolated from a human postoperative infectious lesion in the right maxilla between the lateral incisor and canine and was characterized by polyphasic taxonomic analysis. 16S rRNA gene sequence analysis revealed that the strain ChDC B134(T) belonged to the genus Peptoniphilus, as it showed sequence similarities to Peptoniphilus indolicus KCTC 15023(T) (94.0 %) and Peptoniphilus asaccharolyticus KCTC 3321(T) (93.8 %). The prevalent fatty acids of of strain ChDC B134(T) were C16:0 (20.3 %), C18:1 cis 9 (34.3 %), and C18:0 (13.2 %). The DNA G+C content was 30.9 mol%. The cell wall diamino acid was D-ornithine, which is a property shared by other reference type strains of the genus Peptoniphilus. Based on the results of phenotypic, chemotaxonomic, and phylogenetic analysis, strain ChDC B134(T) (=KCOM 1628(T) = KCTC 15227(T) = JCM 30223(T)) should be classified as the type strain of a novel species of genus Peptoniphilus, for which the name Peptoniphilus mikwangii sp. nov. is proposed.</P>
Eugene Cho,Hyoun Woo Kim,Da Hye Jeong,Seokmyon Lee,Deuk-Sil Oh,김진 조선대학교 치의학연구원 2022 Oral Biology Research (Oral Biol Res) Vol.46 No.3
This study aimed to investigate the antioxidant and anticancer activities of the fermented extract of the dried pericarp of Camellia japonica L. (CJ). The antioxidant activity of the extract after fermentation by Aspergillus oryzae (A. oryzae) at different doses, times, and temperatures was slightly decreased compared with that of the unfermented extract. The total polyphenol content decreased significantly as fermentation time was increased at 30°C, but there was no significant change when the fermentation was performed for 72 hr at different temperatures. The total flavonoid content was not significantly changed by fermentation conditions. Evaluation of the anticancer activity of the A. oryzae-fermented extract revealed that there was no effect on the viability of immortalized 293T cells treated with the extract for 48 hr, but the viability of YD-8 and YD-10B oral cancer cells was significantly reduced. In addition, there was a significant increase in the expression of cleaved PARP and caspase 3, and in apoptotic (annexin V/PI-stained) cells. These results suggest that the water extract of dried pericarp of CJ has no anticancer activity, but gained anticancer activity following fermentation by A. oryzae; furthermore, the fermented extract may be a potential treatment for oral cancer.
Fusobacterium hwasookii sp. nov., Isolated from a Human Periodontitis Lesion
Cho, Eugene,Park, Soon-Nang,Lim, Yun Kyong,Shin, Yeseul,Paek, Jayoung,Hwang, Cheol Ho,Chang, Young-Hyo,Kook, Joong-Ki Springer-Verlag 2015 Current microbiology Vol.70 No.2
<P>In this study, we classified the five strains (ChDC F128(T), ChDC F145, ChDC F174, ChDC F206, and ChDC F300) as a novel species of genus Fusobacterium by DNA-DNA hybridization and multi-locus phylogenetic analysis (MLPA), based on a single sequence (24,715 bp) of 22 concatenated housekeeping genes, with morphological and chemotaxonomic characteristics. DNA-DNA hybridization data showed that the values of genomic relatedness between ChDC F128(T) and each of the other novel strains were ranged from 79.0 to 82.6 %, while those of genomic relatedness between ChDC F128(T) and type strain of each of subspecies of F. nucleatum or Fusobacterium periodonticum were ranged from 40.9 to 54.4 %. MLPA revealed that the 5 strains were clustered as one group and clearly discriminated with F. nucleatum and F. periodonticum with 100 % bootstrap value. The DNA G+C content of the five novel strains were ranged from 26.9 to 27.0 mol%. The cellular fatty acid analysis of clinical isolates and type strains revealed C14:0, C16:0, and cis-9 C16:1 as the major fatty acids. The cell wall peptidoglycan of the 5 strains was comprised of meso-lanthionine. These results show that the 5 strains are novel species and belong to the genus Fusobacterium. Strain ChDC F128(T) (=KCOM 1249(T) = KCTC 5108(T) = JCM 30218(T)) is suggested to be the type strain of a novel species of genus Fusobacterium, for which the name Fusobacterium hwasookii sp. nov. is proposed.</P>
Cho, Eugene,Lee, Jin-Kyung,Lee, Jee-Young,Chen, Zhihao,Ahn, Sun-Hee,Kim, Nam Doo,Kook, Min-Suk,Min, Sang Hyun,Park, Byung-Ju,Lee, Tae-Hoon MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.11
<P>Osteoporosis is caused by an imbalance of osteoclast and osteoblast activities and it is characterized by enhanced osteoclast formation and function. Peptidyl-prolyl cis-trans isomerase never in mitosis A (NIMA)-interacting 1 (Pin1) is a key mediator of osteoclast cell-cell fusion via suppression of the dendritic cell-specific transmembrane protein (DC-STAMP). We found that <I>N</I>,<I>N</I>′-1,4-butanediylbis[3-(2-chlorophenyl)acrylamide] (BCPA) inhibited receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in a dose-dependent manner without cytotoxicity. In addition, BCPA attenuated the reduction of Pin1 protein during osteoclast differentiation without changing <I>Pin1</I> mRNA levels. BCPA repressed the expression of osteoclast-related genes, such as <I>DC-STAMP</I> and osteoclast-associated receptor (<I>OSCAR</I>), without altering the mRNA expression of nuclear factor of activated T cells (<I>NFATc1</I>) and cellular oncogene fos (<I>c-Fos</I>). Furthermore, Tartrate-resistant acid phosphatase (TRAP)-positive mononuclear cells were significantly decreased by BCPA treatment compared to treatment with the Pin1 inhibitor juglone. These data suggest that BCPA can inhibit osteoclastogenesis by regulating the expression of the DC-STAMP osteoclast fusion protein by attenuating Pin1 reduction. Therefore, BCPA may be used to treat osteoporosis.</P>
조유진 ( Eugene Cho ),김현우 ( Hyoun-woo Kim ),백효은 ( Hyo-eun Baek ),김현석 ( Hyeon-seok Kim ),조건웅 ( Geon-ung Jo ),박세이 ( Se-i Park ),오찬진 ( Chan-jin Oh ),오득실 ( Deuk-sil Oh ),김진 ( Jin Kim ) 조선대학교 공학기술연구원 2020 공학기술논문지 Vol.13 No.1
Ten species belonging to the genus Dicranopteris are distributed throughout the world and Dicranopteris pedeta (Houtt.) Nakaike is distributed in Korea. In this study, the antioxidant and anticancer activities of Dicranopteris pedeta aqueous extract was explored. Antioxidant activity of Dicranopteris pedeta aqueous extract was revealed with a range of 38% to 74% in the DPPH radicals test. The total polyphenols and flavonoids contents of aqueous extract were 92.5 ± 8.68 mg GAE/g and 28.04 ± 4.08 mg QE/g, respectively. Protocatechuic acid, gentisic acid, rutin, vanillic acid, hesperidin, oxyresveratrol, quercetin, and naringenin were detected by HPLC. Cytotoxicity test was conducted using MTT assay in normal cells 293T, cancer cells of YD-10B and HeLa. In the cytotoxicity test, aqueous extract did not show toxicity to normal cells but inhibited cancer cell proliferation. These results suggest that aqueous extracts of Dicroanopteris pedeta could potentially be developed as a functional cosmetics or anticancer agents.
탄닌산 분해 효소 처리에 따른 감 추출물 유효성분 변화 및 항산화능 활성 분석
조유진 ( Eugene Cho ),오찬진 ( Chan Jin Oh ),조건웅 ( Geon Ung Cho ),김현석 ( Hyun Suk Kim ),홍주희 ( Joo Hee Hong ),박세이 ( Se I Park ),김현우 ( Hyoun Woo Kim ) 조선대학교 공학기술연구원 2020 공학기술논문지 Vol.13 No.3
Persimmons (Diospyros kaki Thub.) are produced mainly in the southern regions by 188,083 tons on an area of 11,132 ha, but only about 50% of the total is processed and consumed. This study attempted to find a way to increase the value of persimmon. Upon the measurement of the antioxidant activity (DPPH) of the persimmon extract at each site, it was found that an antioxidant activity of about 40% was highest in the immature pulp of the hydrothermal and ethanol (EtOH) extracts. As a result of analyzing 14 active ingredients or phenolic compounds by LC/MS/MS, it was discovered that the content of gallic acid was generally high. Therefore, the index component was selected as gallic acid and was referred to in the experiment. Previously, it was reported that when tannin acid was decomposed, the gallic acid content increased. Meanwhile, the gallic acid content increased by 3-4 times, as a result of Tannase treatment, following the change of the active ingredient in the extract. Based on these results, it is believed that developing a product that is utilized as a functional material is possible by increasing the active ingredient through tannic acid decomposition enzyme treatment of persimmon extract.
Jungheum Cho,Joon Woo Lee,Eugene Lee,Yusuhn Kang,Ha Ra Cho,Dong Yoon Kim,Myoung Jin Ho,Myung Joo Kang,Yong Seok Choi 대한통증학회 2019 The Korean Journal of Pain Vol.32 No.4
Background: To develop a rabbit epidural steroid injection (ESI) model for analyzing steroid retention in the tissue, and to assess the difference in steroid retention in the model according to the location and time elapsed after ESI. Methods: Fluoroscopy-guided ESI was performed using the interlaminar approach between the lowest two lumbar segments in 13 female New Zealand white rabbits. Four rabbits were allocated to each of three different groups according to the time of sacrifice: 3, 7, and 15 days post-ESI; the remaining rabbit was sacrificed immediately post-ESI to obtain baseline data. After sacrifice, two segments were harvested: the lowest two lumbar vertebrae and another two lumbar vertebrae immediately above these. The residual steroid amount (RSA) and residual steroid concentration (RSC) in the collected spinal columns were analyzed. A linear mixed model was used to compare RSAs and RSCs between the injected and adjacent segments, and among the number of days until sacrifice; P < 0.05 was considered statistically significant. Results: Both RSA and RSC of the injected segment were significantly higher than those of the adjacent segment (P < 0.001, both). The RSA and RSC significantly decreased over time (P = 0.009 and P = 0.016, respectively). Conclusions: The developed rabbit ESI model verified that significantly more steroid was retained at the injected segment than at the adjacent segment and the residual steroid decreased over time. This model could be useful not only for comparing current steroid medications, but also for developing new, better steroid formulations.