소모성 질환에 이환된 이유자돈에 대해 Porcine cytomegalovirus DNA Polymerase 유전자 검출과 분석

김지언,김지영,楚佳奇,채미경,장경수,김명철,박창식,전무형 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Porcine cytomegalovirus (PCMV) causes piglet deaths, runting, inclusion body rhinitis, respiratory and reproductive disorders in swine herd, resulting in the significant economic losses in the swine industry. Recently, in the field of pig-to-human xenotransplantation, PCMV has been indicated as a potential xenogeneic pathogen with the risk for infection transmitted from the xenograft donor to the recipient. In this study, PCMV DNA polymerase gene was detected in the sera and nasal swabs from 30 heads of the weaned piglets affected with various wasting diseases and 10 heads of normal healthy piglets using polymerase chain reaction (PCR), and the diagnostic efficacy of PCR was evaluated. The prevalence of the PCR-positive by specimens from the diseased piglets were 83.3% in sera and 33.3% in nasal swabs. In the normal healthy group, the PCR-positive rates of sera and nasal swabs were 40% and 30%, respectively. Ten piglets (25.0%) out of 40 showed the simultaneously positive results in both of sera and nasal swabs. According to this result, it was found that the serum specimen was the most appropriate sample for detection of PCMV by PCR. The nucleotide sequence homology of DNA polymerase gene of PCMV KS1 as compared with the reference strain of PCMV (GenBank accesson no.AF027217) was 99.0%.

대농갱이 암,수의 성장 차이

강언종,이배익,조미영,손상규,김광석,김이청,방인철 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.4

대농갱이는 동자개과의 담수어로 고부가가치를 창출 할 수 있는 새로운 담수 양식 대상종으로 주목받고 있으나 현재까지 성장도 등 양식에 대한 기초정보가 밝혀지지 않았으며, 본 연구에서는 인공으로 생산한 자 ? 치어를 사육하면서 암 ? 수의 성장 차이를 비교 ? 조사하였다. 암수의 정확한 성장 차이를 구명하기 위해 본 실험은 자어 초기 조직검사에 의한 조사와 치어기부터 표지법에 의한 조사로 구분하여 실시하였다. 조직학적 판별에 의한 암수간 성장비교 시험 결과 부화 후 50일까지는 수컷과 암컷의 성장차이는 없는 것으로 나타났으나, 부화 80일 후에는 수컷(체장 72.0±6.7㎜, 체중 3.71±6.7g이 암컷(체장 64.3±6.7㎜, 체중 3.71±0.99g)보다 성장이 빠른 것으로 나타나 대농갱이는 부화 후 50일에서 80일 사이에 암 ? 수의 성장차이가 시작되는 것으로 사료되었다. 부화 80일 이후 조사는 표지법이 개체 성장에 미치는 영향을 조사한 후 본 실험에 착수하였으며 표지구는 대조구와 사이에 유으적인 성장의 차이를 보이지 않았다. 개체간 성장차이는 조직학적 판별 결과와 동일한 경향으로 수컷이 암컷보다 성장이 유의적으로 빨랐으며(P<0.05), 시험 종료 후 암컷과 수컷의 크기는 수컷이 평균 체장 17.57㎝, 체중 52.19g으로 암컷의 평균 체장 11.02㎝, 체중 16.26g보다 체장은 약 1.5배, 체중은 약 3배이상 큰 것으로 나타났다(p=0.05). 체중으로 본 성장곡선은 지수성장을 보였으며, 수컷은 Y=0.0024X^(1.738)(R^(2)=0.985), 암컷은 Y=0.0405X^(1.0316)(R^(2)=0.983)의 지수식에 밀접하게 수렵되었고 성장의 차이는 사육기간이 길어질수록 크게 나타났다. The Ussurien bullhead, Leiocassis ussuriensis, of the family Bagridae has recently been targeted for freshwater culture in Korea. In this paper we investigated sexual dimorphism in body size in rearing condition using specimens produced artificially. Tissue sections of gonads were made between 20 and 80 days after hatching to identify sex of the specimens investigated. A difference in body size between sexes was not observed in fry until they were 50 days old. Male fry 80 days old had attained 81.62f7.21 ㎜ total length, and females 72.84±8.62 ㎜ total length. After 80 days the difference in body size was analysed by tagging. Applying the tag by cutting fin tips had no affect on the growth of the fishes (P< 0.05). The growth in body size of males was significantly faster (P< 0.05) than in females, and males attained about 1.5 times more in standard length and about 3 times more in body weight than females at the end of 320 days. The growth curve of body weight was exponential and the body weight was expressed by Y = 0.0024X^(1.738) (R² = 0.985) and Y = 0.0405X^(1.0316)(R² = 0.983) in male and female, respectively, observed at monthly intervals.

초등학교 3학년 학생의 수학에 대한 인식 태도 연구

박순길,강지우,고대용,김다희,임언미 大邱大學校 師範大學 附設 敎育硏究所 2009 學校敎育硏究 Vol.5 No.1

The purpose of this study was to investigate a study on awareness attitudes of mathematics education in elementary school. This study was conducted with 152 W elementary school of Gwang Ju, 105 H elementary school of Gyeong Ju making up 257 in total. Mean, standard deviation, t-test were done to draw out the conclusions according to different study purposes. The findings of this study were as follows; First, the awareness attitudes of mathematics education was non-significant compared to those in the male-female group. Second, it can be said that the awareness attitude of Gwang Ju toward the elementary students was more positive compared to the Gyeong Ju elementary students. Third, it can be said that the awareness attitude of mathematics attend a lecture group toward the elementary students was more positive compared to the non-mathematics attend a lecture group. 본 연구는 초등학교 3학년 학생들의 수학에 대한 인식태도를 알아보고자 하였다. 본 연구의 대상은 광주광역시 W초등학교 3학년 152명과 경주시 H초등학교 3학년 105명이며, 연구결과를 알아보기 위하여 기술통계와 t검정을 하였으며 연구결론은 다음과 같다. 첫째, 초등학교 3학년 학생들의 수학에 대한 인식태도에서 남녀 간의 차이가 나타나지 않았다. 그러므로 수학에 대한 자신감이나 흥미에서 남녀 간에 차이를 보이지 않았다. 둘째, 지역별로 대도시에 사는 초등학교 3학생들이 중소도시에 사는 초등학교 3학년 학생들보다 인지적 영역에서 차이가 있었으며, 더 긍정적인 인식을 가지고 있었다. 셋째, 수학학원에 다니고 있거나 다닌 경험이 있는 초등학교 3학년 학생들이 학원에 다니지 않은 초등학교 3학년 학생들보다 수학에 대한 인지적 영역, 정의적 영역, 전체인식태도에서 더 긍정적인 인식을 가지고 있었다.

玄蔘의 Saponin 成分 檢索 : on the Saponin of the Radix

남인숙,배병숙,최보향,최인수,최태수,김동언,김언주,정미영,조규옥 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

Crude saponin(100g) were obtained by extracting the radix(5kg) of Scrophalariae koraiensis Nakai. Crude saponin were positive in the Liebermann-Burchard test. We observed 5 sports by TLC using BuOH saturated with H_2O : ethyl acetate : water(4:1:5, upper phase) as solvent and 1% Ce(SO_4)_2 in 10% H_2SO_4 as a color former. We divided into 3 fractions by common column chromatography using BuOH saturated with H_2O : ethylacetate : water(4:1:5, upper phase) as solvent.

Prediction of drug-induced immune-mediated hepatotoxicity using hepatocyte-like cells derived from human embryonic stem cells

Kim, Dong Eon,Jang, Mi-Jin,Kim, Young Ran,Lee, Joo-Young,Cho, Eun Byul,Kim, Eunha,Kim, Yeji,Kim, Mi Young,Jeong, Won-il,Kim, Seyun,Han, Yong-Mahn,Lee, Seung-Hyo Elsevier 2017 Toxicology Vol.387 No.-

<P><B>Abstract</B></P> <P>Drug-induced liver injury (DILI) is a leading cause of liver disease and a key safety factor during drug development. In addition to the initiation events of drug-specific hepatotoxicity, dysregulated immune responses have been proposed as major pathological events of DILI. Thus, there is a need for a reliable cell culture model with which to assess drug-induced immune reactions to predict hepatotoxicity for drug development. To this end, stem cell-derived hepatocytes have shown great potentials. Here we report that hepatocyte-like cells derived from human embryonic stem cells (hES-HLCs) can be used to evaluate drug-induced hepatotoxic immunological events. Treatment with acetaminophen significantly elevated the levels of inflammatory cytokines by hES-HLCs. Moreover, three human immune cell lines, Jurkat, THP-1, and NK92MI, were activated when cultured in conditioned medium obtained from acetaminophen-treated hES-HLCs. To further validate, we tested thiazolidinedione (TZD) class, antidiabetic drugs, including troglitazone withdrawn from the market because of severe idiosyncratic drug hepatotoxicity. We found that TZD drug treatment to hES-HLCs resulted in the production of pro-inflammatory cytokines and eventually associated immune cell activation. In summary, our study demonstrates for the first time the potential of hES-HLCs as an <I>in vitro</I> model system for assessment of drug-induced as well as immune-mediated hepatotoxicity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Generation and characterization of hES-HLCs for evaluation of drug-induced immune cell-mediated hepatotoxicity. </LI> <LI> The secretion of inflammatory cytokines is highly enhanced from APAP-treated hES-HLCs. </LI> <LI> Immune cells are activated and produce pro-inflammatory cytokines by conditioned medium from hES-HLCs cultured with APAP. </LI> <LI> Hepatotoxic results in hES-HLCs are consistent with those of primary human hepatocytes. </LI> <LI> Hepatotoxic drugs such as TZD, and non-hepatotoxic drugs such as aspirin and metformin, are also validated with our drug screening system. </LI> </UL> </P>

배양중 건선 각질형성세포의 증식과 분화양상

김기홍,신동훈,김현석,최종수,김성용,김미진,성언기 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.2

Background : Psoriasis is a common, scaly erythematous disease of unknown etiology, marked by remissions and exacerbations of unpredictable onset and duration. Among many etiologic factors, psoriatic keratinocyte is found to play the most important role. Objeetive : The purpose of this study is to evaluate the hypothesis that the mechanisms) responsible for the almormal proliferation of psoriatic keratinocytes may be located within the cell themselves. Methods : Human epidermal keratinocytes were isolated from lesion(PL) and from uninvolved skin (PN) with chronic plaque-like psoriasis and from the normal skin(NN). Keratinocytes were passaged onto culture vessels without the feeder layer and maintained with serum free medium. Growth rates were measured in secondary cultures by MTT assay and ultrastructural findings of cell differentiation were evaluated with a transmission electron microscope. Results : 1. Keratinocytes from PL reached 50%m confluency in one week compared to two weeks of PN and NN in primary cultures. 2. By the MTT assay, keratinocyte proliferation from PL showed a significantly faster rate than those from PN and NN(p$lt;0.01). But there was no significant difference of keratinocyte proliferation rate between PN and NN(p$gt;0.05). 3. All of the three cell populations(PL, PN, NN) showed variable degrees of cell differentiation during secondary culturing in a serum-free medium. In the PL, however, small, compact basal cells were more prevalent than PN and NN. 4. When keratinocytes underwent differentiation by culturing in DMEM with senun, keratinocytes from PL formed more cell layers with incomplete formation of cornified envelopes suggests the presence of some unknown factors that induce or promote psoriasis. While keratinacytes from PN and NN were characterized by a complete cornified layer as in normal skin. Conclusion : These results indicated that the characteristic hyperproliferation and the defective terminal differentiation of keratinocytes of PL were maintained throughout the culture period.

Dental management for Children with Attention-Deficit/Hyperactivity Disorder

Mi Sun Kim,Soo Eon Lee,Jae Hong Park,Kwang-Chul Kim,Yeong Chul Choi,Sung Chul Choi Asia association of Disability and Oral health 2012 대한장애인치과학회지 Vol.8 No.1

Resveratrol Induces Mitochondrial Apoptosis and Inhibits Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma Cells

Kim, Seong-Eon,Shin, Sang-Hun,Lee, Jae-Yeol,Kim, Chul-Hoon,Chung, In-Kyo,Kang, Hae-Mi,Park, Hae-Ryoun,Park, Bong-Soo,Kim, In-Ryoung Taylor Francis 2018 Nutrition and cancer Vol.70 No.1

Development of accurate dimethyl sulphide primary standard gas mixtures at low nanomole per mole levels in high-pressure aluminium cylinders for ambient measurements

Kim, Mi Eon,Kang, Ji Hwan,Kim, Yong Doo,Lee, Dong Soo,Lee, Sangil BUREAU INTERNATIONAL DES POIDS ET MESURES 2018 METROLOGIA -BERLIN- Vol.55 No.2

<P>Dimethyl sulphide (DMS) plays an important role in atmospheric chemistry and climate change. Ambient DMS is monitored in a global network and reported at sub-nanomole per mole (nmol/mol) levels. Developing traceable, accurate DMS standards at ambient levels is essential for tracking the long-term trends and understanding the role of DMS in the atmosphere. Gravimetrically prepared gas standards in cylinders are widely used for calibrating instruments. Therefore, a stable primary standard gas mixture (PSM) is required for traceable ambient DMS measurement at remote sites. In this study, to evaluate adsorption loss on the internal surface of the gas cylinder, 6 nmol mol<SUP>−1</SUP> DMS gas mixtures were prepared in three types of aluminium cylinders: a cylinder without a special coating on its internal surface (AL), an Aculife IV  +  III-treated cylinder (AC), and an Experis-treated cylinder (EX). There was little adsorption loss on the EX cylinder, whereas there was substantial adsorption loss on the other two cylinders. The EX cylinder was used to prepare 0.5, 2, 5, and 7 nmol mol<SUP>−1</SUP> DMS PSMs with relative expanded uncertainties of less than 0.4%. The DMS PSMs were analytically verified and consistent within a relative expanded uncertainty of less than 1.2%. The long-term stability of the 7 nmol mol<SUP>−1</SUP> DMS PSM was assessed by tracking the ratio of the DMS to the internal standard, benzene. The results showed that the DMS was stable for about seven months and it was projected to be stable for more than 60 months within a relative expanded uncertainty of 3%.</P>

Feasibility study of an optically-stimulated luminescent nanodot dosimeter (OSLnD) in high-energy photon beams

Kim, Jin-Young,Park, Sung-Kwang,Kim, Yon-Lae,Suh, Tae-Suk,Shin, Jung-Wook,Oh, Kyoung-Min,Nam, Sang-Hee,Kim, Jong-Eon,Min, Byung-In,Jo, Sun-Mi,Oh, Won-Young 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol. No.