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Genetic Diversity Analysis of Panax ginseng Genetic Resources using Molecular Markers
Enkhtsetseg Yeruult,Moon Kyo Kim(김문교),Jin Su Gil(길진수),Tsend-Ayush Sarantuya,Mi Sun Lee(이미선),Ju Hyeok Kim(김주혁),Yurry Um(엄유리),Yong Min Kim(김영민),Jae Kyung Byun(변재경),Yi Lee(이이) 한국약용작물학회 2020 한국약용작물학회 학술대회논문집 Vol.2020 No.1
Yi Lee,Moon Kyo Kim,Mi Sun Lee,Juhyeok Kim,Enkhtsetseg Yeruult,Suk-Yoon Kwon,Sol Ji Noh,Seong Won Park 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
Watermelon (Citrullus lanatus) is an important fruit that grows in temperate tropical climates around the world. In this study, quantitative trait loci (QTL) analysis was performed using the genotypes and phenotypes of 87 F2 populations derived from CBARES1 and CBARES2 from the Korean watermelon resources. A total of 37 quantitative traits related to leaves, stems, flowers, and fruits were investigated. The single nucleotide polymorphism (SNP) locus and the insertion or deletion (InDel) locus were found by comparing the nucleotide sequences of the parent generation, CBARES1 and CBARES2. SNP markers were genotyped through resequencing and InDel markers genotyped through PCR and electrophoresis. A linkage map with a total length of 3036.90 cM was constructed consisting of 1,539 SNP markers and 80 InDel markers. InDel data from the actual experimental results were compared with SNP data to confirm the linkage distance and location on the chromosome. As a result, from the 80 InDel markers, 56 were matched to the SNP location and 24 slightly deviated. QTL analysis with the inclusive composite interval mapping (ICIM) method revealed that 37 traits showed significant QTL results in 73 regions of the chromosome. The epistasis QTL analysis was performed to supplement the part that did not show a clear logarithm of the odds ratio (LOD) value due to the additive effect. The linkage map and QTL created in this study are important resources for gene discovery and improvement of watermelon quality.