Carvedilol alleviates testicular and spermatological damage induced by cisplatin in rats via modulation of oxidative stress and inflammation

Ahmed H. Eid,Noha F. Abdelkader,Ola M. Abd El-Raouf,Hala M. Fawzy,Ezz-El-Din S. El-Denshary 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.12

The clinical application of the anticancer drugcisplatin is limited by its deleterious side effects, includingmale reproductive toxicity. In this context, the potentialprotective effect of carvedilol on testicular and spermatologicaldamage induced by cisplatin in male Sprague–Dawley rats was investigated. Carvedilol was orallyadministered at a dose of 10 mg/kg for 2 weeks, and cisplatinwas given as a single intraperitoneal injection of10 mg/kg on the 12th day to induce toxicity. Cisplatinsignificantly reduced reproductive organ weight, spermcount and sperm motility, and increased sperm abnormalitiesand histopathological damage of testicular tissue. Inaddition, it resulted in a significant decline in serumtestosterone as well as levels of testicular enzymatic andnon-enzymatic antioxidants (superoxide dismutase, catalase,glutathione peroxides, and reduced glutathione). Moreover, cisplatin remarkably augmented malondialdehyde,nitric oxide, tumor necrosis factor-a, and nuclearfactor-kappa B contents in testicular tissue. Conversely,carvedilol administration markedly mitigated cisplatin-inducedtesticular and spermatological injury as demonstratedby suppression of oxidative/nitrosative andinflammatory burden, amendment of antioxidant defenses,enhancement of steroidogenesis and spermatogenesis, andmitigation of testicular histopathological damage. Thecurrent study reveals a promising protective action ofcarvedilol against cisplatin-induced reproductive toxicityby virtue of its anti-inflammatory and antioxidantproperties.

Novel Mutations in the Displacement Loop of Mitochondrial DNA are Associated with Acute Lymphoblastic Leukemia: A Genetic Sequencing Study

Yacoub, Haitham Ahmed,Mahmoud, Wael Mahmoud,El-Baz, Hatim Alaa El-Din,Eid, Ola Mohamed,ELfayoumi, Refaat Ibrahim,Elhamidy, Salem Mohamed,Mahmoud, Maged M. Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents approximately 25% of cancer diagnoses among those younger than 15 years of age. Materials and Methods: This study investigated alterations in the displacement loop (d-loop) region of mitochondrial DNA (mtDNA) as a risk factor and diagnostic biomarker for early detection and diagnosis of acute lymphoblastic leukemia. Using mtDNA from 23 subjects diagnosed with acute lymphoblastic leukemia, the first 450 bp of the d-loop region were amplified and successfully sequenced. Results: This revealed 132 mutations at 25 positions in this region, with a mean of 6 alterations per subject. The d-loop alterations in mtDNA in subjects were all identified as single nucleotide polymorphisms in a homoplasmic distribution pattern. Mutant alleles were observed in all subjects with individual frequency rates of up to 95%. Thirteen mutant alleles in the d-loop region of mtDNA occurred with a high frequency. Novel alleles and locations were also identified in the d-loop of mtDNA as follows: 89 G insertions (40%), 95 G insertions (13%), 182 C/T substitutions (5%), 308 C insertions (19%), and 311 C insertions (80%). The findings of this study need to be replicated to be confirmed. Conclusions: Further investigation of the relationship between mutations in mitochondrial d-loop genes and incidence of acute lymphoblastic leukemia is recommended.

Implementation of waste silicate glass into composition of ordinary cement for radiation shielding applications

Mohanad S. Eid,I.I. Bondouk,Hosam M. Saleh,Khaled M. Omar,M.I. Sayyed,Ahmed M. El-Khatib,Mohamed Elsafi 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.4

The aim of this work is to study the radiation shielding properties of cement samples with waste glassincortated into its composition. The mass attenuation coefficient (MAC) of the samples were experimentally determined to evaluate their radiation shielding ability. The experimental coefficient wasevaluated using NaI detector for gamma energies between 59.53 keV and 1408.01 keV using differentradioactive point sources Am-241, Eu-152, Co-60, and Cs-137, and the gamma transmission parametershalf-value layer, mean free path, and transmission factor were calculated. The theoretical coefficient ofthe composites was determined using Geant4 and XCOM software. The results were also comparedagainst Geant4 and XCOM simulations by calculating the relative deviation between the values todetermine the accuracy of the results. In addition the mechanical properties (including Compressive andporosity) as well as the thermogravimetric analysis were tested for the present samples. Overall, it wasconcluded that the cement sample with 50% waste glass has the greatest shielding potential for radiationshielding applications and is a useful way to reuse waste glass.

Biotechnological application of plant growth-promoting endophytic bacteria isolated from halophytic plants to ameliorate salinity tolerance of Vicia faba L.

Mahgoub Hany A. M.,Fouda Amr,Eid Ahmed M.,Ewais Emad El-Din,Hassan Saad El-Din 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.6

Salinity is a major problem affecting crop production all over the world. A wide range of adaptation strategies are required to overcome this problem. Endophytic bacteria can build a symbiotic association with their host to improve host plant salt tolerance. In this study, eighteen bacterial endophyte strains were isolated from two native halophytic plants Arthrocnemum macrostachyum and Spergularia marina, and identified as Bacillus, Brevibacillus, Agrobacterium, and Paenibacillus. These endophytic strains exhibit plant growth-promoting activities including phosphate solubilizing, ammonia production, bio- control of phytopathogen, extracellular enzymatic activities, and indole-3-acetic acid production under normal and salinity stress. A pot experiment was conducted under field conditions to alleviate the harmful effects of soil salinity on bean (Vicia faba L.) by inoculating their seeds with the most potent bacterial isolates Bacillus subtilis (AR5) and Bacillus thuringiensis (BR1). Salinity treatments induced a significant decrease in both growth parameters and metabolic activities, while the activ- ity of antioxidant enzymes and proline content was significantly increased. However, salinity stress induced higher contents of Na+ and decreased contents of N+, P3+, K+, Ca2+, Mg2+, and K+:Na+, it was found that treatment with B. subtilis (AR5) and B. thuringiensis (BR1) individually or in a combination mitigated the effect of salt stress and improved the plant height, shoot dry weights, proline contents, enzymes activities as well enhanced the accumulation of mineral nutrients in shoot plants. Our results concluded that treatment with co-inoculation of B. subtilis (AR5) and B. thuringiensis (BR1) exerted the greatest effect in alleviating the harmful effect of soil salinity stress and can be used as a suitable bio-approach to reclaim salinity-stressed soils.

Multipurpose Treatment of Cellulose-Containing Fabrics to Impart Durable Antibacterial and Repellent Properties

Nabil A. Ibrahim,Ahmed Amr,Basma M. Eid 한국섬유공학회 2020 Fibers and polymers Vol.21 No.3

Development of multifunctional cellulose-containing fabrics has been highly demanded both by the textile finisherand the textile consumer. Herein, proper finishing formulations comprise the following ingredients individually and incombination: a reactant crosslinker (Fixapret® ECO), hydrolysed 3-glycidyl-oxypropyltrimethoxysilane (GPTMS), as a cocrosslinkerand fixing agent, a flurochemical water/oil repellent agent (Asahi Guard® AG-925), silver nanoparticlesdispersion (AgNPs), as antibacterial agent along with metal salt catalyst, especially AlCl3, were applied to various cellulosecontaining fabrics, using a pad-coating impregnation method, to add new multifunctional properties like anti-crease, water/oilrepellent, antibacterial functionality as well as thermal stability. The degree of improvement in the imparted functions isgoverned by type of substrate, extent of modification and/or crosslinking, amount/location/distribution as well as fixation ofthe added active ingredients. Also, the obtained results clearly demonstrated that the created multifunctional properties arenot seriously decreased even after 15 washing cycles. Various characterization techniques were used, and the possibleinteractions among the nominated components and the treated substrates were also suggested.