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M. Kir aly,Z. Hozer,M. Horvath,T. Novotny,E. Perez-Fero,N. Ver 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.2
The mechanical and corrosion behavior of the Russian zirconium fuel cladding alloy E110, predominantlyused in VVERs, has been investigated for many decades. The recent commercialization of a new, optimizedE110 alloy, produced on a sponge zirconium basis, gave the opportunity to compare the mechanicalproperties of the old and the new E110 fuel claddings. Axial and tangential tensile test experiments were performed with samples from both claddings in theMTA EK. Due to the anisotropy of the cladding tubes, the axial tensile strength was 10e15% higher thanthe tangential (measured by ring tensile tests). The tensile strength of the new E110G alloy was 11%higher than that of the E110 cladding at room temperature. Some samples underwent chemical treatment e slight oxidation in steam or hydrogenation e or heattreatment e in argon atmosphere at temperatures between 600 and 1000 C. The heat treatment duringthe oxidation had more significant effect on the tensile strength of the claddings than the oxidation itself,which lowered the tensile strength as the thickness of the metal decreased. The hydrogenation of thecladding samples slightly lowered the tensile strength and the samples but they remained ductile even atroom temperature.
Luciane M. Tomaz,Jean-Marc Lavoie,Marina R. Barbosa,Zahra Farahnak,Cristiani G. Lagoeiro,Natalia S.S Magosso,Sérgio E. A. Perez 한국운동영양학회 2016 Physical Activity and Nutrition (Phys Act Nutr) Vol.20 No.2
Purpose: This study investigated the effects of ovariectomy (Ovx) and 12 weeks of resistance training (RT) on gene expression of GLUT2, the main glucose transporter in the liver, and on PPARγ, a transcription factor known to target GLUT2 expression. Methods: Forty Holtzman rats were divided into 5 groups: Sham-sedentary (Sed), Sham-RT, Ovx-Sed, Ovx-RT, and Ovx-Sed with hormone replacement (E2). The RT protocol consisted of sessions held every 72 h for 12 weeks, during which the animals performed 4 to 9 vertical climbs (1.1 m) at 2 min intervals with progressively heavier weights (30 g after the fourth climb) tied to the tail. The E2 silastic capsule was inserted into the rats’ backs 48 hours before the first RT session. Results: In addition to liver fat, GLUT2 protein levels and PPARγ transcripts were increased (P < 0.05) in Ovx compared to Sham-Sed animals, suggesting increased hepatic glucose uptake under estrogen deficient conditions. RT and E2 in Ovx rats decreased liver fat accumulation as well as GLUT2 and PPARγ gene expression to the level of Sham-Sed animals. Conclusion: The results of this study suggest that liver GLUT2 as well as PPARγ expression in Ovx rats are accompanied by increased fat accumulation and glucose uptake, thus providing a substrate for increased de novo lipogenesis. RT appears to be an appropriate exercise model to circumvent these effects.
Gonzalez-Perez, C.J.,Vargas-Arispuro, I.,Aispuro-Hernandez, E.,Aguilar-Gil, C.L.,Aguirre-Guzman, Y.E.,Castillo, A.,Hernandez-Mendoza, A.,Ayala-Zavala, J.F.,Martinez-Tellez, M.A. The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.2
The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.
Tania Perez-Perez,Arletty Funcada-Martinez,Ania Cabrera-Diaz,Luis E. Guerra-Diaz,Deny Oliva-Merencio,Zhenia Milan,Ileana Pereda-Reyes 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.5
Large pig farms generate enormous volumes of wastewater without proper treatment before discharge. High-rate anaerobic reactors as the expanded granular sludge bed (EGSB) are rarely reported for that purpose. In this study, an EGSB reactor with Cuban natural zeolite addition was evaluated for treating piggery wastewaters at lab scale. This study was conducted at different organic loading rate (OLR) ranging from 0.32 to 6.13 gCOD/Ld with a constant hydraulic retention time (HRT) of 12 h. The evolution of several control and operational parameters as the soluble chemical oxygen demand (COD) demonstrated a proper bioreactor performance to treat piggery wastewater. The results showed that the addition of natural zeolite at 40 gzeo/Lreactor in EGSB reactor results in COD efficiencies over 70%, despite of the fluctuations of fed wastewater characteristics. The kinetic study demonstrates that, the modified Stover-Kincannon model was found to be appropriate for EGSB reactor. The effluent soluble COD was predicted with high determination coefficient. The maximum removal rate constant (Umax) and saturation value constant (KB) were reported for a first time for the EGSB reactor with values of 10.2 and 11.8 gCOD/Ld, respectively. Methane yield for both, batch and continues experiments, were in the range from 214.5 to 299.8 mLCH4/gCOD.
( C. J. Gonzalez-perez ),( I. Vargas-arispuro ),( E. Aispuro-hernandez ),( C. L. Aguilar-gil ),( Y. E. Aguirre-guzman ),( A. Castillo ),( A. Hernandez-mendoza ),( J. F. Ayala-zavala ),( M. A. Martinez 한국미생물생명공학회(구 한국산업미생물학회) 2019 한국미생물·생명공학회지 Vol.47 No.2
The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.
Brito-Carreón Cesar Armando,Zavala-Maldonado Karla,Suárez-Andino Erika Ivette,David Randy E.,Perez- Ramírez Gerardo,Diaz-Badillo Alvaro,Muñoz María de Lourdes 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
Currently, there are no antiviral drugs that effectively reduce the risks and treat the symptoms associated with dengue virus (DENV). Consequently, efforts remain primarily focused on transmission reduction. One such effort concerns DENV receptors in mosquito vectors Aedes aegypti and Aedes albopictus. Despite a lack of direct evidence demonstrating the binding of DENV to cells in mosquito vectors, one putative DENV binding protein has been α-enolase. To develop a deeper understanding, this study tested whether DENV proteins bind to enolase localized in the cytoplasmic membrane of C6/36 cells using both anti-enolase-specific antibodies, and by colocalization analysis, using confocal microscopy. Additionally, to probe the interaction of enolase with the DENV E protein, we performed a docking analysis using PatchDock and FireDock software packages. Study results demonstrate that the DENV E protein interacts with enolase in the plasma membrane of C6/36 cells of Ae. albopictus. Specific anti-enolase antibodies were found to inhibit DENV infection of these cells. Moreover, enolase was found to be localized to the cytoplasmic mem brane, cytoplasm, and nucleus. These combined findings suggest that enolase participates in the entrance mechanism of DENV into vector cells.
( Cecilia Perez-cruz ),( Carlos N. Cano-gonzalez ),( Jose Fuentes ),( Nagamani Balagurusamy ),( Carolina E. Vita ),( Roque A. Hours ),( Cristobal N. Aguilar ),( Sebastian F. Cavalitto ),( Juan C. Cont 한국키틴키토산학회 2018 한국키틴키토산학회지 Vol.23 No.3
Aspergillus niger biomass, an industrial by-product of citric acid fermentation is an emergent source of glycoderivatives with applications in biofuel, cosmetics, feed, energy, food, medicine, and nanotechnology. In this study, the effect of purified neutral protease for deprotenization of fungal biomass studied at various levels (0, 5, 10, 20 and 40 U/100 mg of biomass) and the saccharification of fungal biomass was evaluated with amylolytic enzymes and chitosanases. The efficiency of deproteinization of fungal biomass was based on the enzyme concentration and contact time. Protease at a concentration of 20 U/100 mg of dry biomass and with a contact time of 8 h achieved 30% final deproteinization. No effect on saccharification of A. niger biomass was observed by treatment with purified amylolytic enzymes. Meanwhile, the endo- and exo-chitosanases treatment yielded 54 g of g reducing sugars (equivalent to amino sugars)/ kg of fungal biomass, which can be employed for tailor-made carbohydrate production.
Jesus Beltran-Perez,Gregory Ramsey,Jorge E. Lopera 소화기인터벤션의학회 2019 International journal of gastrointestinal interven Vol.8 No.2
This case report shows an effective esophageal stent placement via retrograde (transgastric) approach under fluoroscopic guidance in the interven-tional radiology suite. This alternative can be evaluated and offered to patients that suffer from a firm esophageal occlusion before a major proce-dure such as surgery can be considered. Thus, decreasing major surgery complications and improving the quality of life.
Vapour permeation of VOC emitted from petroleum activities: Application for low concentrations
G. Rebollar-Perez,N. Lesage,P. Moulin,E. Carretier 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.4
This study deals with the application of vapour permeation on the abatement of air polluted with VOC,from low to medium concentrations. Toluene, butadiene and propylene were chosen as model compounds. On a first part of the study, the permeation of single VOC, binary and ternary VOC/air mixtures was performed at different flow rates and VOC concentrations. The permeate flux, permeance,enrichment factor, separation efficiency and the recovery were determined. The experimental results were compared to those obtained with a mathematical model, allowing the calculation of the membrane area required to eliminate 95% of the feed VOC content.