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Ian Paul Navea,Maria Stefanie Dwiyanti,Chenie Zamora,Katreena Titong,Michael J Thomson,Sunghan Kim,Hee-Jong Koh,Joong Hyoun Chin 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
New QTLs were identified for high grain yield with long panicle in rice. A total of 137 F15 recombinant inbred lines (RILs) derived from a cross between Dasanbyeo (Tongil) and TR22183 (japonica), together with the parents were evaluated for 16 agronomic traits at IRRI in dry and wet seasons under different phosphorus (P) and irrigation conditions. A linkage map was constructed using 236 polymorphic markers in 384-plex Bead Xpress indica-japonica single nucleotide polymorphism (SNP) platform. P and water effects were significant in both wet and dry seasons. Both parents and RILs showed varying degree of sensitivities to scarcities in water and phosphorus in terms of panicle length. Collocating with 20 yield-related QTLs, the panicle QTLs on chromosomes 1 (pl01) ,2 (pl02), 9 (pl09), and 11 (pl11) under low P and rainfed conditions were identified. RILs with TR22183 allele at pl11 showed longer panicle length under low P input rainfed condition in dry and wet seasons. The whole-genome sequences of the two varieties are being compared to design the molecular markers for fine-mapping and candidate gene identification. Based on Nipponbare MSU 7.0 annotation, a total of 1464 genes with predicted function were identified within the four QTL regions. Candidate genes identified in other studies for QTLs under low P and water conditions, such as calmodulin and dehydrin genes, were targeted for designing molecular markers for fine-mapping and expression analysis. Pyramiding the panicle length QTLs correlating with yield QTLs will provide an opportunity of improving yield traits.
Gadang Priyotomo,Tamara Emylia Suci Nurarista,Yanyan Dwiyanti,Bening Nurul Hidayah Kambuna,Arini Nikitasari,Siska Prifiharni,Sundjono 한국부식방식학회 2022 Corrosion Science and Technology Vol.21 No.6
The objective of this study was to determine whether Cymbopogon citratus extract as a corrosion inhibitor from natural tropical resources could prevent corrosion of carbon steel in sulfuric acid solution. Inhibitory action of this extract was investigated using electrochemical methods such as potentiodynamic polarization and electrochemical impedance spectroscopy (EIS). Those methods revealed corrosion rate, efficiency of inhibition, and adsorptions isotherm values when the extract was added to the sulfuric acid solution at concentration up to 500 ppm with various immersion time at ambient temperature. Results revealed that higher concentration of the extract and longer immersion time decreased the corrosion rate of carbon steel whereas the inhibition efficiency of the extract was increased up to 97.25%. The value of charge transfer resistance was increased significantly by adding the extract at concentration up to 500 ppm with an immersion time of 60 minutes. The type of the extract was a mixed inhibitor. It could inhibit the corrosion process in both anodic and cathodic sides electrochemically. Results of this study suggest that the mechanism of adsorption on the surface of carbon steel is related to Langmuir adsorption isotherm.
Kusumadewi Yulita,Susila,Henti Hendalastuti Rachmat,Fifi Gus Dwiyanti,Tika D. Atikah,Atok Subiakto,Bayu Pratama,Titiek Setyawati,Wita Wardani,Rizki Ary Fambayun,Nawwall Arrofaha,Irsyad Kama 한국산림과학회 2022 Forest Science And Technology Vol.18 No.4
Dalbergia latifoliais commercial tropical tree species known for its beautiful heartwood,mainly used for furniture and musical instrument. High market demand has put concerns onits sustainability and conservation aspects in Indonesia. Ninety-five specimens ofD. latifoliawere collected from eight populations of Java, Lombok, and Sumbawa Island to study intra-specific variability and diversity using SRAP. One hundred and eighty SRAP loci with 54.03%± 4.35% polymorphism obtained from PCR amplification of 10 primer combinations, with theaverage PIC for these primers of 0.28. Genetic diversity and variability measures were calcu-lated using GenAlEx software indicating a relatively low-mid level of percentage of poly-morphic loci (PPL) (54.03%), effective number of alleles (Ne) (1.255), Shannon informationindex (I) (0.242), and heterozygosity (He) (0.156) on average. The highest value (I¼0.309, He¼0.196) was observed in population P2CJ (Central Java), followed by West Java of P1WJ(I¼0.300, He¼0.191) and West Sumbawa of P7Wsumb (I¼0.257, He¼0.169), while thelowest (I¼0.202, He¼0.129) was found in West Lombok (P4WL). The genetic relationshipswere measured from genetic distance and identity from the two-pairwise calculation, PCoA,and STRUCTURE analysis. The relative homologous population is found between populationsCentral and West Java of P2CJ and P1WJ (Nei uD¼0.031), suggesting these populationshave the same origin and formed one population, while the most distinct (Nei uD¼0.157)was recorded between Sumbawa regency (P6Sumb) and East Java (P3EJ). The results fromPCoA showed that all individuals are placed in three groups almost in accordance with theirrespective islands, i.e. a group comprised inclusively trees from Sumbawa island, a group ofLombok and some Central Java trees, and a group of trees from Java with the exclusion ofsome trees of Central Java that were clustered with trees from Lombok island. This result wasalso supported by STRUCTURE analysis, which showed that Sumbawa, Lombok, and West-East Java populations formed their respective clusters, and Central Java population contains amixture of West-East Java and Lombok populations. This may suggest evidence of complexorigin, thus needing further study to clarify. It is recommended that the populations with thehighest value of genetic diversity (Central and East Java) are to be maintained as the sourceof the gene pool for genetic enrichment programs.
Delima Engga Maretha,Dini Afriansyah,Dewi Susilo Wati,Mashuri Masri,Ade Rizky Dwiyanti,Muhammad Ifham Hanif,Slamet Wardoyo 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.4
Premature aging can occur due to excessive sun exposure which causes hyperpigmentation problems and causes brown or black patches on the skin. Duku (Lansium domesticum Corr.) contains flavonoids and vitamin C compounds that are effective in protecting the skin from sun exposure. This study aims to see the benefits of the extract of the seeds and peel of duku fruit (Lansium domesticum Corr.) On the amount of melanin pigment in the skin of mice (Mus musculus) as an antihyperpigmentation so that the dapjekal is a skin pen. Group P0 was a negative control, group P1 was exposed to sunlight for 20 days, group P2 mice were exposed to duku seed extract and then exposed to sunlight for 20 days, group P3 were exposed to duku peel and exposed to local sunlight for 20 days, P4 mice were exposed to sunlight 20 days, 21–30 days exposed to peel extract, and P5 mice exposed to sunlight 20 days, 21–30 days exposed to seed extract. The results showed that the P0 group had the amount of melanin pigment with an average of 7 grains, the P1 group: 68.4, P2 group: 26.6, P3 group: 29, P4 group: 7.2, and group P5 group: 12. The conclusion is that exposure to sunlight the amount of melanin pigment and offers the extract of the peel and seeds of duku fruit can reduce the amount of melanin pigment.
Applying High-Throughput SNP Genotyping and Sequencing to Accelerate Progress in Rice Breeding
Michael J. Thomson,Christine Jade Dilla-Ermita,Maria Ymber Reveche,Maria Dwiyanti,Geraldine Malitic,Geisha Sanchez,Nadia Vieira,Venice Juanillas,Ramil Mauleon,Bertrand Collard,Joong Hyoun Chin,Eero Ni 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
We are currently developing a high-throughput single nucleotide polymorphism (SNP) genotyping service at IRRI to accelerate progress in rice breeding by providing rapid and cost-effective marker services. SNP marker development and validation is being performed based on cloned genes and QTLs, GWAS hits, and whole genome sequence data to identify predictive SNP markers at important genes for key traits for the breeding programs. Trait-based and targeted SNPs are being deployed in sets of 24 and 96 SNPs on a Fluidigm EP1 system. At the same time, 384 SNP sets and a 6K SNP chip developed by Susan McCouch at Cornell University are being used for higher density genome scans on an Illumina system. Genotyping by sequencing (GBS) approaches with 96 and 384 barcoded samples per sequence lane are also being evaluated in comparison to SNP array technology based on the number of loci, call rates, turnaround times, and cost per sample. An efficient sample processing workflow with an integrated LIMS is also being optimized to enable high throughput genotyping with sample tracking to minimize errors. Moreover, web-based SNP data analysis tools have been deployed through the IRRI Galaxy workbench to speed up SNP data analysis. Future efforts will focus on large-scale deployment of GBS across breeding materials to enable QC genotyping, tracking of donor introgressions, and integration of genome-wide prediction into the variety development pipelines. The large-scale application of high-density markers will help transform IRRI’s rice breeding programs and increase the rate of genetic gain towards developing high-yielding, stress-tolerant varieties for target environments and market segments