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Screening of rice drought tolerant germplasms and drought tolerant QTL mapping
Dongjin Shin,Tae-Heon Kim,Sang-Ik Han,Ji-Yoon Lee,Youngbo Son,Sung Hwan Oh,Yeon-Jae Hur,Saisbeul Lee,Jun-Hyun Cho,Jong-Hee Lee,You-Chun Song,Min-Hee Nam,Dong-Soo Park,Yeong-Up Kwon 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
In here, we screened drought tolerant varieties with modified leaf water loss rate assay and visual drought tolerant phenotype in the greenhouse conditions with more than 800 varieties. Among these varieties, Samgang, Gumei4 and Apo showed the lowest of leaf water loss rate and strong drought tolerant phenotype. To identify drought QTLs with Samgang variety, we developed the doubled-haploid (DH) population consist of 101 lines derived from a cross the drought tolerant cultivar Samgang and the drought sensitive cultivar Nagdong. To score the drought phenotype degrees of this population, we withheld water for 6 weeks and treated the watering for 7 days. After watering, visual phenotype was observed 1 to 9 degree according to the standard evaluation system for rice, IRRI. Drought sensitive parent Nagdong was almost died and was scored as 9 degree, while tolerant parent Samgang showed slightly leaf tip drying phenotype and was scored as 3 degree in our experimental conditions. Three main QTLs were detected on chromosome 2, 6, and 11 with this visual phenotype. We also measured relative water contend of these population under drought stress conditions, and got one main QTL on chromosome 11. The QTL loci on chromosome 11 with flanking markers RM26755-RM287 has a function for visual phenotype and relative water content under drought conditions.
16S rDNA Sequence and Biochemical Characters of a Korean Isolate of Xenorhabdus nematophilus
Dongjin Ji,Youngkeun Yi,Kim, Yonggyun Korean Society of Applied Entomology 2004 Journal of Asia-Pacific Entomology Vol.7 No.1
A Gram-negative bacterium, Xenorhabdus nematophilus, is mutualistically associated with entomopathogenic nematode, Steinernema carpocapsae. A bacterial isolate was obtained from the nematode collected in Korea and identified as X nematophilus Kl (Park et al., 1999). This study was focused on the characterization of the bacterial isolate by 16S ribosomal RNA gene (rDNA) sequence, fatty acid composition, carbon source utility, and insecticidal activity that were compared with those of other related bacteria. The universal ribosomal primers were used to amplify 1,536 bp rDNA. The 16S rDNA sequence showed high homology (equation omitted 97%) with those of foreign X nematophilus isolates, but slightly lower homologies with those of other species in the entomopathogenic bacteria. Though there was variation in fatty acid composition between two X nematophilus isolates, they shared the major fatty acid as palmitic acid (16:0), while that of X poinarii and X bovienii was oleic acid (18:1). Carbon source utility characters of the Kl isolate differed with those of the French isolate. Two X nematophilus had high insecticidal activity against the beet armyworm, Spodoptera exigua, but the Kl isolate was 18 times as potent as the French isolate. These taxonomic comparisons suggest that the Kl isolate had unique physiological characters in fatty acid composition, carbon source utility, and insecticidal activity in the species.
Development of marker-free transgenic rice expressing wheat storage protein, Glu-Dx5
Soo-Kwon Park,Woon-Ha Hwang,DongJin Shin,Ji-Yoon Lee,Jun-Hyeun Cho,Myung-Hee Kim,Min-Hee Nam,Dong-Soo Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Development of transgenic plant increasing crop yield or disease resistance is good way to solve the world food shortage. However, the persistence of marker genes in crops leads to serious public concerns about the safety of transgenic crops. In the present study, we developed marker-free transgenic rice inserted high molecular-weight glutenin subunit (HMW-GS) gene (Dx5) from the Korean wheat cultivar ‘Jokyeong’ using Agrobacterium-mediated co-transformation method. The Dx5’s own promoter was used for protein expression. Two expression cassettes comprised of separate DNA fragments containing only the Dx5 and hygromycin resistance (HPTII) genes were introduced separately into Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Dx5 or HPTII was infected into rice calli at a 3: 1 ratio of EHA105 with Dx5 gene and EHA105 with HPTII gene expressing cassette. Then, among 270 hygromycin-resistant transformants, we obtained 27 transgenic lines inserted with both the Dx5 and HPTII genes into the rice genome. We reconfirmed integration of the Dx5 gene into the rice genome by Southern blot analysis. Wheat Dx5 transcripts in T1 rice seeds were examined with semi-quantitative RT-PCR. Protein expression of the Dx5 was analyzed with Western blot using polyclonal antibody recognising x-type of glutenin subunits in T1 seeds. It was suggested that the protein-processing system was conserved between rice and wheat. Finally, the marker-free plants containing only the Dx5 gene were successfully screened at the T1 generation.
[PB-0061] Assessment of Mesocotyl Elongation in Doubled Haploid Rice (Oryza sativa L.)
Simon Alibu(Simon Alibu ),Nkulu Rolly Kabange(Nkulu Rolly Kabange ),Youngho Kwon(Youngho Kwon),Ji-Yun Lee(Ji-Yun Lee),So-Myeong Lee(So-Myeong Lee),Jin-Kyung Cha(Jin-Kyung Cha),Dongjin Shin(Dongjin Shi 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
Kim, Dongjin,Lee, Jun Su,Lee, Gayoung,Choi, Byung-Hyun,Ji, Mi-Jung,Park, Sun-Min,Kang, Misook Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.7
In this study the effects of adding alkaline-earth (IIA) metal oxides to NiZr-loaded Zeolite Y catalysts were investigated on hydrogen rich production by ethanol steam reforming (ESR). Four kinds of alkaline-earth metal (Mg, Ca, Sr, or Ba) oxides of 3.0% by weight were loaded between the $Ni_6Zr_4O_{14}$ main catalytic species and the microporous Zeolite Y support. The characterizations of these catalysts were examined by XRD, TEM, $H_2$-TPR, $NH_3$-TPD, and XPS. Catalytic performances during ESR were found to depend on the basicity of the added alkaline-earth metal oxides and $H_2$ production and ethanol conversion were maximized to 82% and 98% respectively in 27($Ni_6Zr_4O_{14}$)3MgO/70Zeolite Y catalyst at $600^{\circ}C$. Many carbon deposits and carbon nano fibers were seen on the surface of $30Ni_6Zr_4O_{14}$/70Zeolite Y catalyst but lesser amounts were observed on alkaline-earth metal oxide-loaded 27($Ni_6Zr_4O_{14}$)3MO/70Zeolite Y catalysts in TEM photos after ESR. This study demonstrates that hydrogen yields from ESR are closely related to the acidities of catalysts and that alkaline-earth metal oxides reduce the acidities of 27($Ni_6Zr_4O_{14}$)3MO/70Zeolite Y catalysts and promote hydrogen evolution by preventing progression to hydrocarbons.
하동진(Dongjin Ha),지현철(Hyunchul-Ji),유재근(Jaegun-Yu),윤광수(Kwangsoo Yoon),이태봉(Taebong Lee) 한국자동차공학회 2011 한국자동차공학회 학술대회 및 전시회 Vol.2011 No.11
This paper presents complex eigenvalue analysis of commercial vehicle air disc brake system. To increase a reliability of a brake finite element model, the mode shapes and natural frequencies of the individual brake components are obtained by comparing form modal analysis and modal test. (The modal analysis and test shows that difference of the natural frequency is very small.) As a result of squeal analysis identified unstable modes of air disc brake system, a similar dynamo noise test. Additional, confirmed the final improvement model through parameter study that decrease the brake squeal.