GH_3 세포(rat somatomammotropic tumor cell)에서 TRH가 Phospholipase D 활성에 미치는 효과

김동선,김태화,이창범,안유헌,윤미섭,한중수 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: GH_3 세포는 TRH에 반응하여 세포막의 수용체와 G 단백, PI-PLC, PKC를 활성시켜 성장호르몬 및 프로락틴을 분비한다고 알려져 있다. PLD는 phosphatidylcholine을 phosphatidic acid(PA)와 choline으로 가수분해하는 효소로서 세포의 증식과 호르몬 분비에 관여한다. 본 연구는 GH_3 세포에서 아직 알려지지 않은 TRH의 PLD 활성에 대한 영향을 규명하고자 하였다. 방법: GH_3 세포를 1.5×10^6씩 분주하고 [^3H] myristate로 표지한 다음에 0.3% 알코올로 전 처치하였다. TRH 등의 시험제를 처치한 후에 메탄올로 반응을 종결하고 세포에서 총 지질을 추출하였다. PLD 활성은 박층크로마토그래피를 이용하여 총 [^3H] phospholipid에서 [^3H] phosphatidylethanol의 비율로 구하였다. 결과: TRH (1μM)의 처치 시에 PLC 활성은 44배 증가하였다. PLD 활성은 TRH (1μM), mastoparan (5μM), PMA(500nM)를 30분간 처치 시에 각각 1.9, 1.5, 2.2배 증가하였다. TRH(1μM)의 시간에 따른 PLD 활성 변동은 15, 30, 60, 120, 240분에 각각 142%, 170%, 172%, 160%, 115%의 증가를 보였다. 결론: GH_3 세포에서 TRH의 호르몬 분비와 세포증식의 신호전달 기전으로서는 PLC 활성뿐 아니라 PLD의 활성도 관여함을 시사한다. Backgroud: GH_3 cells are a well characterized and widely used model used for the in vitro study of growth hormone (GH) secretion. Thyrotropin releasing hormone (TRH) binds to receptors belonging to the family of G protein-coupled receptors, and secrets both GH & prolactin. Phospholipase D (PLD) is and enzyme that hydrolyses phosphatidylcholine to yield phosphatidic acid and choline, and plays important roles in cellular proliferation and hormonal secretion. To elucidate the pathway of the action of TRH in GH_3 cells, we investigated the activities of PLC and PLD in GH_3 cells treated with TRH or phorbor 12-myristate 13-acetate (PMA). Methos : GH_3 cells were labeled with [^3H] myristate, followed by incubation of with 0.3% ethanol, prior to before the addition of the agonists. The total lipids were extracted from the harvested cells following treatment with the agonists. The PLD activity was assessed by measuring [^3H] phosphatidylethanol from the [^3H] phospholipid using thin layer chromatography. Results : TRH (1μM) stimulated the PLD activity by 44-fold over that of the control values. TRH (1μM), mastoparan (5μM), and PMA (500μM) for 30 minutes increased PLD activity by 1.9, 1.5 and 2.2 fold, respectively, in comparison to the controls, The PLD activities after 15, 30, 60, 120 and 240 min treatments of TRH (1μM) were 142%, 170%, 172%, 160% and 115%, respectively. Conclusion : These results suggest that TRH stimulates not only PLC activity, but also the PLD activity in GH_3 cells (J Kor Soc Endocrinol 17:465∼472, 2002).

화학공정 위험영향 평가기술에서의 다중요소분석기법을 이용한 사고시나리오 산정에 관한 전략

김구회,이동언,김용하,안성준,윤인섭 한국화재소방학회 2001 한국화재소방학회논문지 Vol.15 No.4

현재 전세계적으로 공장내(on-site)뿐만 아니라 공장외지역(off-site)에 대한 사고영향평가의 필요성이 대두되고 있으며, 공장외지 역에 대한 영향평가 수행후 이에 대한 적절한 비상계획을 제출하도록 하고 있고, 국내에서도 종합적위험관리체계(IRMS ; Integrated Risk Management System)를 PSM이나 SMS와 더불어 시행 준비중에 있다. 그러나 공장외지 역에 대한 위험영향 평가시 가장 먼저 결정되어야할 가상시나리오에 대한 기준이 체계적으로 마련되어 있지 않아 사용자나 분석자에 따라 평가결과가 다양하게 나오며, 또한 공정에서 발생할 수 있는 사고의 이상원을 정확하게 파악하지 못한다는 단점이 있다. 따라서 본 연구에서는 이러한 가상시나리오를 결정하는데 필요한 공정요소를 분석한 후, 분석결과에 따라 발생 가능하고, 먼저 고려되어야 할 시나리오를 산정할 수 있도록 전략을 제시하고자 한다. 분석된 공정요소들은 요소에 따른 가중치를 부여하여 위험등급을 결정한 후 등급에 따른 위험영향 평가를 수행하도록 한다. 분석의 결과는 가상시나리오의 신뢰성을 향상시킴으로써 위험영향평가가 과대평가되는 것을 방지하고, 공정의 설계나 비상계획의 수립시 효과적이고 적절한 대책마련을 유도할 수 있다. This article proposes a strategy for producing accident scenarios in quantitative risk, which is performed in process design or operation steps. Present worldwide chemical processes need offsite risk assessment as well as on-site one. Most governments in the world require industrial companies to submit the proper emergency plans through off-site risk assessment. Korea is also preparing for executing Integrated Risk Management System along with PSM and SMS. However, there have been no systematic approaches and criteria for generating virtual accident scenarios, and it made impossible to get a unified or coherent assessment resell. Without the result, causes of accidents cannot be corralled precisely, To get over these shortcomings, this study analyzed process elements and then proposed a strategy for selecting and generating the accident scenario that is most likely to happen and should be foremost considered. The analyzed process elements are ranked and risk grades determined. According to the grades, risk assessment is performed. The result of analysis enhances the reliability of the generated risk scenario, and prevents some risks from being overestimated. The result should be helpful in process design and emergency planning. Keywords : Off-site risk assessment, Risk management program, Worst case scenario, ES(Equipment Screening) Method, Accident scenario selection

둔상성 비장 손상의 선택적 치료

이봉석,박동일,안우섭,서정욱,오민구,배성한,정병욱 대한외상학회 1994 大韓外傷學會誌 Vol.7 No.1

Until recently, total splenectomy has been considered as a safe operation for splenic injuries under the misconception that the spleen could not heal spontaneously and the spleen was not necessary for the maintenance of life. Concurrent with the knowledge for immunologic function of spleen, a renewed int,erest has been evolved in the technique of splenic salvage, including splenorrhaphy, partial splenectomy, nonoperative management, and autotransplantation of splenic fragments. Authors have reviewed 47 patients with splenic injury admitted to the Department of Surgery, Dong-Guk University Hospital during the period from June, 1987 to December, 1993. The patients were classified in accordance with Cogbill s splenic organ injury scale which defines five categories for the splenic injury. Among them 30 patients were treated by splenectomy, 2 patients by partial splenctomy, 11 patients by splenorrhaphy, and 4 patients by nonoperative management. In conclusion, nonoperative management is a safe treatment option if the following criteria are satisfied: 1) absolute hemodynamic stability or rapid hemodynamic stabilization after fluid resuscitation : 2) lack of other serious intraabdominal injuries: 3) Class I or ll splenic injury by Cogbill s method.

Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

외상성 간손상의 선택적 치료

이준희,박기영,박동일,안우섭,서정욱,오민구,배성한,정병욱 대한외상학회 1995 大韓外傷學會誌 Vol.8 No.1

With the increment of the role of CT scan, nonoperative treatment is the recent trend in hemodynamically stable patients in the management of traumatic liver injuries. To investigate the trend of treatment and to suggest the possible criteria for nonoperative treatment, we reviewed 50 patients with traumatic liver injuries who were treated in the Department of Surgery, Dong-Guk University Hospital from January 1989 to June 1994. Of 50 patients, 35 patients were treated with nonoperative management and 15 patients were treated with operative management. Based on CT findings, we classified those 50 patients into each grade of liver injuries ranging from grade 1 to 5 and we classified t,he degree of hemoperitoneum ranging from grade 0 to gracle 3+, and we compared those results with the clinical outcome. The operation rate according to liver injury grade didn't increase with the increment of the grade, and the degree of hemoperitoneum corresponds well to the rate of operation. In the nonoperative cases, there was only one complication and there was no mortality. In conclustion, even major liver injuries including grade 4 by CT scan can be treated with nonoperative rnanagement if the patients are hemodynamically stable and they do not have combinded intraabdominal organ injury.

급성 충수염의 천공여부를 예측할 수 있는 판별함수

민석기,이준희,정병욱,오민구,배성한,박동일,안우섭,서정욱 대한대장항문학회 1996 Annals of Coloproctolgy Vol.12 No.1

항-Jk^b 항체에 의한 심한 용혈성 수혈부작용 2예

허미나,한복연,한규섭,유원희,안혁,이동엽,오창완 大韓輸血學會 1997 大韓輸血學會誌 Vol.8 No.1

갑상선암 수술 중 발생하는 의도하지 않은 부갑상선 절제의 임상적 의의

김영균,김정구,이동호,이혜경<SUP>1<.SUP>,유영경,안창준,Young Gyun Kim,M.D.,Jeong Gu Kim,M.D.,Dong Ho Lee,M.D.,Hae Gyung Lee,M.D.<SUP>1<.SUP>,Young Kyoung Yoo,M.D. and Chang Joon Ahn,M.D. 대한갑상선-내분비외과학회 2006 The Koreran journal of Endocrine Surgery Vol.6 No.1

Purpose: Many surgeons intend to preserve all of the parathyroids during a thyroid operation; however, they have had the unpleasant experience of achieving unintentional parathyroidectomy. We studied the risk factors for unin-tentional parathyroidectomy and whether these unintentional parathyroidectomies caused symptomatic hypocalcemia. Methods: We conducted a retrospective review of the medical records and pathologic reports of 95 papillary carcinoma patients who underwent operations between January 1994 and December 2003. We statistically analyzed the correlation between unintentional parathyroidectomy and the risk factors such as tumor size, capsular invasion, including extra-thyroidal extension, and the operation method. Results: Of the total 95 procedures, 14 (14.7%) produced patho-logy reports stating that incidental parathyroid tissue was identified within the thyroidectomy specimen. The majority of the 14 reported cases contained only a single focus of inci-dental parathyroid tissue; however, one of these thyroidectomy specimens contained two foci of parathyroid tissue. The factors such as tumor size, capsular invasion and extensive surgery were not correlated with the prevalence of unintentional parathyroidectomy. There was no association of unintentional parathyroidectomy with postoperative hypocalcemia (P=.449).Conclusion: Unintentional parathyroidectomy is not associated with symptomatic postoperative hypocalcemia. The factors such as tumor size, capsular invasion or extensive surgery are not risk factors for unintentional parathyroidectomy. (Korean J Endocrine Surg 2006;6:17-21)

70세 이상 고령의 대퇴 경부 골절에서의 무시멘트 양극성 고관절 반치환술

최원식(Won Sik Choy),안재훈(Jae Hoon Ahn),정학재(Hack Jae Jeong),이승훈(Seung Hun Lee),양동현(Dong Hyun Yang),감병섭(Byoung Sup Kam),구제윤(Je-Yoon Koo) 대한정형외과학회 2008 대한정형외과학회지 Vol.43 No.6

목적: 고령의 대퇴 경부 골절 환자에서 시행한 무시멘트 양극성 고관절 반치환술의 추시 결과를 임상적 및 방사선학적으로 분석하고자 하였다. 대상 및 방법: 70세 이상의 대퇴경부 골절로 SPH-C2<SUP>®</SUP> stem을 이용한 무시멘트 양극성 반치환술을 받은 환자 88명중 최소 2년 이상 추시가 가능하였던 80예를 대상으로 하였다. 남자 19예, 여자 61예이고, 평균 연령은 76세, 평균 추시 기간은 37개월이었다. 임상적 평가는 Harris hip score와 술후 서혜부 및 대퇴 통증과 술후 보행상태의 변화를, 방사선학적으로는 스템의 안정성, 정렬의 변화와 침강, Gruen 구역별 방사선 투과선, 골내막 신생골 형성, 골용해 등을 분석하였다. 결과: 최종 추시시 Harris hip score는 80.2점이었고 임상적으로 유의한 중등도 이상의 통증은 1예였다. 80예 중 55예(68.7%)에서 술 후 보행기능의 저하 없이 회복되었다. 추시 방사선 사진상 대퇴 스템 안정성은 골성 안정 삽입 47예(58.7%), 섬유성 안정 삽입 33예(41.3%)였으며 골내막 신생골 형성은 30예(37.5%)였다. 모든 예에서 골용해 소견은 없었으며 유의한 정렬의 변화냐 대퇴 삽입물의 진행성 수직 침강 없이 안정성을 유지하였다. 결론: 70세 이상의 대퇴 경부 골절에서 시행한 무시멘트 양극성 고관절 반치환술의 2년 이상 추시상 임상적 및 방사선학적으로 만족스러운 단기 결과를 얻을 수 있었다. Purpose: The purpose of this study was to evaluate clinicoradiological outcomes after cementless bipolar hemiarthroplasty in elderly patients with femoral neck fractures. Materials and Methods: Eighty hips-all in patients greater than 70 years of age-were followed for more than 2 years after undergoing cementless bipolar hemiarthroplasty with a tapered cementless stem (Lima SPH-C2<SUP>®</SUP>). The mean age was 76 years, and the mean follow-up period was 37 months. The Harris hip score and postoperative hip pain were analyzed clinically. The radiological results were assessed using various radiological indices. Results: At last follow-up, the mean Harris hip score was 80.2 points. There was one case of significant hip pain. Fifty-five cases (68.7%) showed no decrease in ambulation capacity postoperatively. Radiologically, there were 47 cases (58.7%) of bone ingrowth and 33 cases (41.3%) of stable fibrous fixation. There were no cases of osteolysis, and 30 cases (37.5%) exhibited new bone formation around the stem. All stems were stable without significant alignment change or progressive subsidence. Conclusion: Short-term outcomes proved to be satisfactory in elderly patients undergoing cementless bipolar hemiarthroplasty for femoral neck fractures.

말초혈액조혈모세포채집술 671 예 시행경험

황동희,허미나,박경운,신수,김양현,신희영,안효섭,박선양,한규섭 大韓輸血學會 2000 大韓輸血學會誌 Vol.11 No.2