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      • 고성능 실리콘 산화질화막 제조

        남인탁,김동현,김웅수 강원대학교 부설 석재복합신소재 제품연구센터 2000 석재연 논문집 Vol.5 No.-

        본 연구에서는 RF/DC 마그네크론 스퍼터링 시스템과 RTA를 이용하여 열처리를 하므로써 실리콘산화질화막(SiOxNy) 제조를 시도 하였다. 우선 상온에서 스퍼터로 실리콘(100) 기판 위에 실리콘산화막(SiO_2)을 제조한 후 900℃에서 질소분위기로 열처리를 하여 박막을 제조한 후 물리적, 화학적 특성들에 대하여 알아보았다. XRD 패턴을 확인해 본 결과 질소와 실리콘산화질화물의 피크가 관찰되지 않았다. 이는 EDX의 선분석과 XPS의 관찰에서도 같은 결과가 나타났다. AFM을 이용해 표면을 살펴 본 결과 실리몬(100) 기판의 영향으로 글라스 기판과는 달리 일정한 방향으로 증착되었으며 열러치 후 다소 부드러운 표면이 관찰되었음을 알 수 있었다. 암모니아(NH_3)와는 달리 스퍼터링의 플라즈마를 이용한 안정적인 질소가스의 이온화가 쉽게 이루어지지 않았던 것과 마찬가지로 RTA를 이용한 열처리에서도 질소가스의 반응성은 매우 낮은 것으로 사료된다. In this study, the grows of silicon oxynitride(SiOxNy) by using RF/DC sputtering method and RTA*rapid thermal annealing) method. Silicon oxynitride(SiOxNy) thin films were annealed at 900℃ in N_2 gas. From XRD results, It was not observed that the peak of the films enhance as the increase of annealing temperature and time. This result was certified by EDX and XPS analysis. From AFM results, the surface roughness were decreased with annealing. The results can be used as basic study for development of high ability insulator.

      • 금수봉과 장태산 일대의 식생 군락에 따른 조류상

        남동하,김정수,구태회 경희대학교 환경연구소 2001 環境硏究 論文集 Vol.10 No.-

        This study is to investigate the diversity of the birds and avifauna in and around Mt. Kumsoo and Mt. Jangtae with focusing on forest type. Mt. Kumsoo consists mainly of natural forest, whereas man-made plantations were found in Mt. Jangtae. Bird diversity was evaluated according to the richness of species. Species richness was higher in Quercus mongolica, Q. variabilis, and Q. serrata-Q. variabilis communities than in Metasequoia glyptostroboides and Picea abies communities, and higher in Castanea crenata, P. koraiensis, Eucommia ulmoides, and P. densiflora communities than in Q. acutissima and Pinus densiflora communities. Nine species of bird discovered in communities of Q. mongolica, Q. variabilis, and Q. serrata-Q. variabilis were not found in M. glyptostroboides and P. abies communities; one species was found in M. glyptostroboides and P. abies communities but not in Q. mongolica, Q. variabilis, and Q. serrata-Q. variabilis communities, and fourteen species were present in all flora. Difference in habitat-use is important because the effect of reforestation on the avian diversity should not be examined only according to forest type, but also should examine the proportion of plantation in each area.

      • 재조합 Saccharomyces cerevisiae에서 Inulinase와 Invertase의 발현과 분비에 미치는 배양조건의 영향

        남수완,신동하,김연희 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

        The effects of medium pH and culture temperature on the expression and secretion and secretion of inulinase and invertase were investigated with recombinant Saccharomyces cerevisiae cells. These cells were obtained by transformation of 2μ-based plasmids pYI10 and pYS10 which contain Kluyveromyces marxianus inulinase gene (INU1A) and S. cerevisiae invertase gene (SUC2), respectively, in the downstream of GAL1 promoter. The expression level and localization of inulinase and invertase were not affected significantly by the initial medium pH: secretion efficiencies of inulinase and invertase into the medium were about 90% and 60%, respectively, in the pH ranges of 4.0 to 6.5. However, the expression and secretion of both enzymes were strongly dependent on the culture temperature. The highest expression (7.7 units/mL) and secretion (6.7 units/mL) of inulinase were observed at 28℃ and 30℃. As a consequence of decreased localization of inulinase in the periplasmic space, the secretion efficiency increased from 68% at 20℃ to 95% at 35℃. The total expression level and secretion efficiency of invertase increased from 19 units/mL and 55% at 20℃ to 25 units/mL and 68% at 35℃, respectively. Irrespective of the culture temperature, the invertase activity in the cellular fraction (periplasmic space and cytoplasmic fractions) was kept constant at around 33∼45%.

      • Saccharomyces cerevisiae에서 Trichoderma Endoglucanase의 발현과 분비

        남수완,김병우,신동하,김재범,신지원,정대균,정춘수 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1

        The endoglucanase gene, egl6, of Trichoderma sp. was connected with the yeast ADH1 promoter, and the resultant plasmid, pVT-C4, was introduced into three S. cerevisiae host strains (YNN27, 2805, and SEY2102). Among each 80 transformants, the cell growth and expression level of endoglucanase were compared in test-tube cultivation, and three respective transformants for each host cells showing the highest expression level and cell growth were selected. When three recombinant yeast cells were batchwise cultivated for 48 hr in flask, the total activities of endoglucanase expressed were about 1140 unit/1 with 2805/pVT-C4, 1020 unit/l with SEY2102/pVT-C4, and 590 unit/l with (YNN27/pVT-C4. Irrespective of host strain, about 80% of the expressed endoglucanase was detected in the extracellular medium. In addition, it was also found that the recombinant enzyme was secreted into the culture medium as two major forms of lightly and heavily glycosylated proteins.

      • 어류의 유전육종과 산업에의 응용에 관하여

        김동수,남윤권 濟州大學校海洋硏究所 1993 해양과환경연구소 연구논문집 Vol.17 No.-

        Over the decade, rapid progress has been made in the application of gene manipulation technology to aqaculture for the genetic improvement of aquacultured species. Two basic fields of genetic improvement involve the endocrinal control such as control of spawning seasons, induced and multiple spawning and cryopreservation of gametes, and the gene manipulation using chromosome engineering, sex reversal, hybridization, selective breeding and gone trasnfer techniques. Chromosome engineering of induced polyploidey, gynogeneris and androgenesis have been extensively investigated in fish. The major goal of thease stueies has been to develop methods for the incressing productibility by controlsex and by rapid establishment of inbreeding lines. Sex reversal techniques has been applied to the species which has sex related significant differences in growth, behavour pattern, breeding time, body shape and size etc. so, culture of monosex population is highly desirable. Pyusiological sex reversal by hormonal treatment or genetic sex reversal by matings between specipic genotypes have been carried out. Hybridization in fish are performed in the hope of buliding the desirable characters, according to some parameters such as viaility, growth potential, sexual maturation and disease resistance. Maternal or paternal genomes can be manipulated before and/or after hererospecipic inseminations. Selective breeding has been carried out to improve the performances or obtain fish products most desirable to the producer by modifying genotypic frequences using specific mating system such as inbreeding or outbreeding and by changing alle frequences toward ones more favorable to the beeder's golds through accumulation of the favored alles. Gene transfer technology has been introduced, aimed at the production of transgenic fish showing the improved charateristics and performances such as enhanced growth, disease resistance and cold resistance in their environment. Researches of transgenesis in fish are focused on the identification of desiable piscine genes, development of expression vector, optimization of introducing techniques, stable expression and transmission to germ line.

      • CA 125치가 증가된 난소종괴 환자에서 악성 감별을 위한 CA 15-3과 TAG 72의 효용성

        이수미,김영탁,정동근,남주현,목정은 울산대학교 의과대학 1992 울산의대학술지 Vol.1 No.1

        The purpose of our study was to evaluate whether the combined use of serum tumor markers, CA 15-3 and TAG 72 could improve the efficacy for discriminating malignant from benign ovarian masses in patients with elevated serum CA 125 levels. Serum CA 15-3 and TAG 72 were preoperatively measured by immunoradiometric assay in 78 patients with pelvic masses who had elevated serum CA 125 levels above 35 U/ml. In 32 patients with epithelial ovarian cancers, serum CA 125 levels were elevated above 65 U/ml in 97% and serum CA 15-3(>30U/ml) and TAG 72(>10 u/ml) levels were elevated in 59% and 50%, respectively, suggesting relatively low sensitivity of CA 15-3 and TAG 72. However, in 41 patients with benign ovarian tumors, elevations of these two markers were detected only in 7% and 5%, respectively, and particularly, both markers were not elevated among all the aptients over 50 years of age. Coordinate elevations of CA 125(>35U/ml) and CA 15-3(>30U/ml) or TAG 72(>10 U/ml) could discriminate epithelial ovarian cancers from benign masses with a sensitivity of 72% and specificity of 90%. Among patients over 50 years of age, sensitivity and specificity were increased to 79% and 100%, respectively. It is concluded that the combined use of CA 15-3 and TAG 72 with CA 125 could obtain an improved efficacy for discrimination of epithelial ovarian cancer, especially among patients over 50 years of age.

      • 播種量 差異에 따른 濟州在來 동부의 生育反應, 收量 및 粗成分 變化

        趙南棋,金東賢,宋昌吉,劉哲受,高東煥 濟州大學校 亞熱帶農業硏究所 1999 亞熱帶農業硏究 Vol.16 No.-

        ABSTRACT This study was accomplished to look into the response of main growth characters, yield and chemical composition of Cheju local cowpea on Cheju Island based on the difference between seeding rate. The study took place between May and September, 1998. Number, of days to flowering tended to be delayed as the seeding rate increased. Plant length was the longest at 6kg/10a treatment and the next one was at 5kg/10a treatment. The shortest came from 2kg/10a treatment, 275 cm, 271 and 220cm respectively. Number of branchs and stem diameter became weaker as the seeding rate increased. Fresh forage yields per lOa was the heaviest which weighed 6,073kg at 6kg/10a treatment and 5,840kg at 5kg/10a treatment respectively. The rest decreased gradually. Dry matter yields per lOa, crude protein yields per lOa and total digestible nutrient yields per lOa showed a similar tendency to fresh yields per lOa. The percentage of crude protein and crude fat got higher as the seeding rate increased, but the percentage of crude ash and crude fiber rather tended to decrease. Nitrogen free extract and total digestible nutrient percentages were similar to the change of crude protein percentage. SPAD reading values were the highest at 6kg/10a and 5kg/10a treatment.

      • 확률론적 해석법에 의한 연직배수 영향인자 민감도 분석

        유남재,전상현,정길수,김동건 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.B

        A probabilistic analysis model, one of reliability analysis methods introducing the concept of probabilistic variables, was developed to investigate the uncertainty of dominant factors influencing the degree of consolidation in the radial consolidation theories. Based on the developed probabilistic analysis model, sensitivity study of those factors was performed to find their trends of affecting the degree of consolidation in the vertical drain method. Various radial consolidation theories, Proposed by Barron(1948), Hansbo(1979), Yoshikuni(1979) and 0noue(1988), were used for this parametric study with the influencing factors such as size of smear zone, reduction ratio of permeability in the smear zone, discharge capacity, permeability for horizontal flow and coefficient of consolidation for horizontal flow. As results of this sensitivity study, for the given consolidation theory, contribution of each factor to the degree of consolidation was figure out and compared to each other. For the given value of each factor, the sensitivity to the degree of consolidation in the various theories was evaluated and their applicability and limitations were assessed.

      • IMT-2000/WLAN/DMB 단말기용 광대역 PIFA 설계 및 SAR 분석

        최동근,신찬수,김남 충북대학교 컴퓨터정보통신 연구소 2004 컴퓨터정보통신연구 Vol.12 No.2

        본 논문에서는 IMT-2000/WLAN/DMB 단말기용 광대역 PIFA(Planar Inverted-F Antenna)를 설계하였다. 일반적인 PIFA 구조의 단점인 협대역 특성을 개선하기 위하여 미앤더 구조를 변형한 두 개의 branch 형태를 사용하였다. 안테나의 크기를 효율적으로 줄이기 위하여 단락스트립을 사용하였고, 미앤더 형태에 따른 방사패치를 가지는 PIFA를 제안하였다. 설계 제작된 안테나의 -10dB 반사 손실 대역폭은 38.2 %(1.84~2.71 GHz)로 IMT-2000/WLAN/DMB 대역을 모두 만족하는 광대역 특성을 얻었다. 전자파 흡수율의 감소량을 알아보기 위해 실제로 폴더형 구조를 가지는 휴대폰에 제안된 안테나를 탑재하여 1 g 및 10 g 평균 첨두 SAR를 측정한 값과 시뮬레이션 결과를 비교하였다. 그 결과는 아주 유사하게 나왔으며, 1 g 및 10 g 평균 첨두 SAR 제한치인 1.6 W/kg, 2 W/kg보다 낮은 값을 얻었다. This paper proposed a new broadband PIFA(Planar Inverted-F Antenna) for IMT-2000/WLAN/DMB terminal. Two branches type changed meander structure was utilized in order to improve the characteristics of PIFA which usually has a narrow band. The shorting strip between the ground plane and the antenna and meander-type radiation patches were used in order to minimize the size of the antenna. The -10 dB return loss bandwidth of a realized antenna was 38.2 %(1.84~2.71 GHz), which contains the broadband with triple band. And the simulated and measured values of 1 g and 10 g averaged peak SAR on human head caused by the triple band PIFA mounted on folder-type handsets were analyzed and discussed. As a result, the measured 1 g and 10 g averaged peak SARs of PIFA was similar to simulated values and were lower than the 1.6 W/kg, 2 W/kg of 1 g and 10 g averaged peak SAR limits.

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