Aflatoxin B_1의 검출을 위한 효소면역측정법의 개발

손동화,박애란,서병철,김진철,이인원,남영중,허우덕 한국산업미생물학회 1992 한국미생물·생명공학회지 Vol.20 No.2

효소면역측정법에 의한 aflatoxin B_1(AFB_1)의 정량법을 개발하기 위하여, 항체를 생산 정제하여 분석법을 확립하고 직접법과 간접법(direct/indirect competitive ELISA)의 특성 및 문제점을 비교·검토하였다. Bovine serum albumin(BSA)을 carrier protein으로 한 AFB_1-1-(0-carboxymethyl)oxime-BSA를 토끼에 면역하여 항AFB_1항혈청을 생산하였다. 정량 침강반응에 의하여 항혈청으로부터 항BSA항체를 제거하고 황산암모늄 침전법 및 DEAE-Sephadex A-50 이온교환 크로마토그래피를 통하여 순도 높은 IgG항체를 정제하여 항AFB_l항체로 사용하였다. 이 항체는 2×10^6이상의 높은 항체가를 갖고 있었으며, AEB_1의 유사독소인 G_2, B_2, G_1에 대하여는 3∼34%의 낮은 교차반응성을 보여 특이성이 비교적 높았다. 직접법 및 간접법의 분석조건을 확립한 후, AFB_1 표준품에 대하여 작성한 표준곡선으로부터 각 방법에 의한 AFB_1의 검출농도는 각각 0.2∼20 및 1∼10,000 ng/㎖(ppb)로 나타나, 직접법이 간접법보다 검출농도의 범위는 좁으나 감도가 더 높았다. 직접법은 분석의 안정성, 간편성, 신속성 등의 면에서 간접법보다 실용화에 적합한 것으로 나타났다. In order to develop an enzyme-linked immunosorbent assay(ELISA) for detecting aflatoxin B_1(AFB_1), we produced and purified antibodies, thereafter established and evaluated methods of direct and indirect competitive ELISA. Anti-AFB_1 antisera, produced by immunizing rabbits with AFB_1-1-(0-carboxymethyl)oxime-bovine serum albumin conjugate(AFB_1-BSA), were removed of anti-BSA antibodies by quantitative precipitation reaction and further purified by ammonium sulfate precipitation and DEAE-Sephadex A-50 ion exchange chromatography. Purified IgG fractions were used as anti-AFB_1 antibodies. The antibodies, whose titer was determined extremely high above 2×10^6, showed low cross-reactivity of 3∼34% against AFB_1 analogues such as G2, B2, and Gl. From the standard curves of direct and indirect competitive ELISA for AFB_1, the detection ranges were found 0.2∼20 and 1∼10,000 ng/㎖(ppb) respectively. In their sensitivity, stability, simplicity, and rapidity, the direct method was more suitable than the indirect method for practical use.

Synthesis of Dense WSi2 and WSi2-xvol.%SiC Composites by High-Frequency Induction Heated Combustion and Its Mechanical Properties

( Dong Young Oh ),( Hwan Cheol Kim ),( Jin Kook Yoon ),( In Yong Ko ),( In Jin Shon ) 대한금속재료학회 ( 구 대한금속학회 ) 2006 METALS AND MATERIALS International Vol.12 No.4

A method to synthesize the dense silicide WSi2 and WSi2-xvol.%SiC composites within two minutes in one step from W, Si and C elemental powders was investigated. Simultaneous combustion synthesis and densification were carried out under the combined effect of an induced current and mechanical pressure. Highly dense WSi2 and WSi2-xvol.%SiC (x=10, 20, 30) with relative density of up to 97% were produced under the simultaneous application of a 60 MPa pressure and the induced current on the reactant powders. The average grain size of WSi2 in WSi2-xvol.%SiC (x=0, 10, 20, 30) were in the region of 6.9, 6.1, 5.2, and 5.0㎛, respectively. The respective Vickers hardness values for these materials were 1375, 1540, 1710, and 1845㎏/㎟. From indentation crack measurements, the fracture toughness values were calculated to be 3.3, 3.8, 4.4, and 5.1MPa·m(1/2), respectively.

Fabrication of Ultra fine WC-Ni Hard Materials by Rapid Sintering Process

Hwan-Cheol Kim,Dong-Young Oh,In-Jin Shon 한국분말야금학회 2004 한국분말야금학회 학술대회논문집 Vol.2004 No.1

(1) Using high-frequency induction heating sintering and spark plasma sintering method, the densification of WC-Ni hard materials was accomplished using ultra fine power of Ni and WC. (2) Nearly fully dense WC-Ni could be obtained within 1 min. (3) Relative density and mechanical properties of WC-Ni obtained by HFIHS were high than those obtained by SPS. And WC grain size made by HFIHS was smaller than that made by SPS. (4) The fracture toughness and hardness values of WC-8Ni, WC-10Ni, and WC-12Ni made by HFIHS were , respectively for 60MPa and an induced current for 90% output of total capacity, 15KW. (5) The fracture toughness and hardness values of WC-8Ni, WC-10Ni, and WC-12Ni made by SPS were , respectively for 60MPa and the electric current of 2500 A

Pancreatitis, Panniculitis, and Polyarthritis Syndrome with a Fatal Course

( Uri Shon ),( Dea Kwan Yun ),( Gi Hyun Seong ),( Dong Yoon Lee ),( Myung Hwa Kim ),( Byung Cheol Park ) 대한피부과학회 2022 Annals of Dermatology Vol.34 No.1

흉요추 및 요추 골절에서 단순 촬영과 3차원 컴퓨터 단층 촬영 간의 시상면 지표의 측정오차

김동수(Dong-Soo Kim),김용민(Yong-Min Kim),최의성(Eui-Sung Choi),손현철(Hyun-Chul Shon),박경진(Kyoung-Jin Park),조병기(Byung-Ki Cho),박지강(Ji-Kang Park),이현철(Hyun-Cheol Lee) 대한정형외과학회 2012 대한정형외과학회지 Vol.47 No.3

목적: 척추 골절의 수술여부 판정에 있어 Cobb각, 압박률 등 시상면 지표가 중요한 요소이나, 그동안은 단순 촬영만으로 이를 측정하였다. 본 연구에서는 최근 도입된 3차원 computed tomography (CT)에서의 시상면 지표와 종래의 단순 촬영상에서의 지표 측정치의 차이를 알아보고자 하였다. 대상 및 방법: 흉요추 및 요추 골절 환자 45명(남자 21명, 여자 24명)의 단순 촬영과 3차원 CT에서 시상각과 척추체 높이를 측정하였다. 시상각은 골절 추체의 상위 척추체와 하위 척추체와의 Cobb각을 측정하였다. 골절 추체의 전후방 척추체 높이를 측정하여 이를 토대로 전후 설상비를 산정하였다. 결과: 단순 촬영의 시상각은 평균 13.1±14.3°, 3차원 CT는 평균 8.2±13.0°로 단순 촬영이 약 4.9°의 후만각이 더 있는 것으로 측정되었으며 이는 통계적으로 유의한 차이를 보였다(p<0.001). 전후설상비는 단순 촬영이 평균 65±17%, 3차원 CT가 평균 73±17%로 유의한 차이를 보였다(p<<0.001). 최초 단순 촬영상 후만 변형이 클수록 단순 촬영과 3차원 CT 간의 시상각차 및 전후 설상비차는 더 크게 나타났다. 결론: 흉요추 및 요추 골절의 단순 촬영과 3차원 CT 간의 시상면 측정값에서 유의한 차이를 보였고, 단순 촬영의 측정치가 더 후만 변형이 큰 것으로 측정됨을 관찰하였다. Purpose: For decision making in the management of vertebral fractures such as operation or not, sagittal parameters like Cobb angle and wedge compression ratio are important. Plain radiography had been the only image tool for measuring such parameters until 3D computed tomography (CT) became popular recently. In this study, we investigated the measurement discrepancy between plain radiography and 3D CT. Materials and Methods: Plain radiography and 3D CT of 45 thoracolumbar and lumbar fracture patients (male=21, female=24) were evaluated. We measured sagittal angle and vertebral height on lateral radiography and sagittal CT. Sagittal angle was measured between the upper body and lower body of fractured vertebrae. Anterior and posterior heights were measured to assess anteriorposterior (AP) wedge ratio. Results: The sagittal angle of plain radiography (13.1±14.3o) was signifi cantly larger than that of 3D CT (8.2±13.0o) by 4.9o (p<0.001). AP wedge ratio of plain radiography was on average 65±17%, which was signifi cantly lower than the 3D CT (73±17%) by 8% (p<0.001). The severer the initial kyphotic deformity, the more discrepancy of results between the two methods was observed. Conclusion: Signifi cant discrepancy was observed in sagittal features of fractured vertebra between plain radiography and 3D CT. Measured values of plain radiography showed more kyphotic features of the fractured body.

한국 한 중소 도시 지역의 우울 장애의 유병률 및 관련 요인

이동윤(Dong-Youn Lee),이철순(Cheol-Soon Lee),박철수(Chul-Soo Park),한규희(Kyu-Hee Hahn),손진욱(Jin-Wook Shon),차보석(Bo Seok Cha),김봉조(Bong-Cho Kim) 대한사회정신의학회 2008 사회정신의학 Vol.13 No.2

목 적 중소도시 진주에 거주하는 일반인구를 대상으로 하여 우울 장애의 유병률과 관련 인자를 조사하기 위해 본 연구를 시행하였다. 방 법 중소도시 진주에 거주하는 남자 528명(39.3%), 여자 815명(60.7%), 총 1,343명을 연구대상으로 하였고 자기 보고 형태인 K-CES-D를 사용하여 우울 장애의 유병률과 관련 인자를 조사였다. 결 과 대상군 중 19.6%가 우울 장애로 진단 되었는데, 연령, 경제 수준, 결혼 상태에 따라 유병률의 차이를 보였으며, 그 외 성별, 교육 수준에 따른 유병률의 차이는 통계적으로 유의하지 않았다. 연령과 결혼 상태가 우울 장애의 관련 인자로 조사 되었다. 이중 연령은 남, 녀 모두에서 유의한 관련이 있었지만, 결혼 상태의 경우 남자에서만 우울 장애의 관련 인자로 조사되었다. 고 찰 본 연구는 일반 인구의 우울 장애 유병률을 알아 볼 수 있었고, 우울 장애와 관련된 여러 가지 역학적 정보는 효과적인 보건 정책의 수립에 다양하게 사용될 수 있을 것이다. Objectives:The objective is to investigate the prevalence and correlates of depression in the population at a smallmedian city, Jinju. Method:Total numbers of this study subjects were 1343. Male were 528(39.3%) and female were 815(60.7%) living in a small-median city, Jinju. The data was collected through self reports in 2007. We investigated the prevalence and correlates of depression using a cut-off score of 21 by K-CES-D. Results:19.6% of samples(20% in female and 19.1% in male) were diagnosed as depression. Although the prevalence of depression was not different by sex and educational level, it was different by age, monthly income and marital status. Multivariate logistic regression analysis showed that the risk factor for depression was age and marital status. Age was significantly associated with depression both in men and women, but marital status was associated with depression only in men. Conclusion:This study revealed the prevalence of depression on the general population-base. This epidemiological information should be used to inform and implement equitable and effective health policies.

CD4/CD8 double-negative early-stage mycosis fungoides with CD30 expression

( Uri Shon ),( Kyujin Yeom ),( Dea Kwan Yun ),( Byung Cheol Park ),( Dong Yoon Lee ),( Mi Soo Choi ),( Myung Hwa Kim ) 대한피부과학회 2021 대한피부과학회 학술발표대회집 Vol.72 No.2

Pityriasis lichenoides et varioliformis acuta-like toxic reaction after pembrolizumab treatment for invasive thymoma

( Uri Shon ),( Kyujin Yeom ),( Dea Kwan Yun ),( Myung Hwa Kim ),( Dong Yoon Lee ),( Mi Soo Choi ),( Byung Cheol Park ) 대한피부과학회 2021 대한피부과학회 학술발표대회집 Vol.72 No.2

Efficacy and safety of oral alitretinoin in various refractory dermatologic diseases

( Uri Shon ),( Dea Kwan Yun ),( Gi Hyun Seong ),( Myeong Jin Park ),( Dong Yoon Lee ),( Byung Cheol Park ),( Myung Hwa Kim ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: Patients with dermatologic diseases that are refractory to conventional treatments including topical or systemic corticosteroids, immunosuppressant, and retinoids need a new treatment option. There are limited reports investigating the efficacy of alitretinoin for various diseases. Objectives: To evaluate the efficacy and safety of oral alitretinoin for various refractory dermatologic diseases in real-world experience. Methods: We retrospectively reviewed 168 patients treated with oral alitretinoin for various dermatologic diseases such as psoriasis, contact dermatitis, hand eczema, and pityriasis rubra pilaris. Treatment response was measured based on the physician’s global assessment scale and adverse events (AEs) based on medical records. Results: Patients with mean age of 46.6±14.7 took oral alitretinoin for 26.1±27.6 weeks. The overall response rate (very good or excellent improvement) was 49.5%. Response rate was the highest (74.4%) in the eczema disease group. 116 (69.0%) patients experienced AEs, and 35 (20.8%) patients discontinued the treatment due to AEs. Most AEs were reversible. Headache (43.5%) was the most common AE, followed by decreased serum free T4 (10.7%), and increased triglyceride level (7.7%). Conclusion: Despite its limited approval for severe chronic hand eczema, alitretinoin can be a relatively safe and effective option for various refractory dermatologic diseases.

GPGPU를 이용한 비디오 기반 실시간 화재감지 알고리즘 구현

손동구(Dong-Koo Shon),김철홍(Cheol-Hong Kim),김종면(Jong-Myon Kim) 한국컴퓨터정보학회 2014 韓國컴퓨터情報學會論文誌 Vol.19 No.8

본 논문에서는 많은 양의 연산량을 요구하는 비디오 기반 4단계 화재감지 알고리즘의 실시간 처리를 위해 범용 그래픽 처리 장치 (general-purpose graphics processing unit, GPGPU)를 이용한 병렬 구현 방법을 제안한다. 또한 GPGPU 기반 화재감지 알고리즘의 효용성을 확인하기 위해 범용 고성능 CPU와의 성능을 비교하였다. SXGA(1400×1050) 해상도의 화재 비디오 5개를 이용해 모의실험 결과, GPGPU기반 화재감지 알고리즘은 CPU 구현보다 약 6.6배 더 높은 성능을 보였으며, 평균 프레임 당 30.53ms의 실행시간이 소요되어 실시간 처리(초당 30프레임)가 가능함을 보였다. This paper proposes a parallel implementation of the video based 4-stage fire detection algorithm using a general-purpose graphics processing unit (GPGPU) to support real-time processing of the high computational algorithm. In addition, this paper compares the performance of the GPGPU based fire detection implementation with that of the CPU implementation to show the effectiveness of the proposed method. Experimental results using five fire included videos with an SXGA (1400×1050) resolution, the proposed GPGPU implementation achieves 6.6x better performance that the CPU implementation, showing 30.53ms per frame which satisfies real-time processing (30 frames per second, 30fps) of the fire detection algorithm.