Highly Efficient Photocatalytic Studies on Bi36 Fe2 O57 Ceramic Synthesized by Chemical Route

Manish Kumar Verma,Vinod Kumar,Upakar Patel,Vishnu Shankar Rai,Anup Kumar,Arup Kumar De,Aditya Kumar Prajapati,Dinesh Prajapati,Kedar Sahoo,Tapas Das,N. B. Singh,K. D. Mandal 한국전기전자재료학회 2023 Transactions on Electrical and Electronic Material Vol.24 No.5

Bi36 Fe2 O57 (BFO) polycrystalline ceramic was synthesized by economical chemical route. The single-phase formation of BFO ceramic was confirmed by powder X-ray diffraction studies. Nanosized formation of BFO ceramics established by XRD and TEM analysis. The bimodal distribution of grain size is observed with size ranging from 50 to 600 nm. It observed that the Bi36 Fe2 O57 photocatalyst exhibited higher catalytic activity for the degradation of Methylene Blue (MB) under visible-light irradiation The oxidation state of elements present in the BFO ceramic, was confirmed by XPS studies. The pseudocapacitive nature of BFO ceramic was observed by cyclic Voltammetry. The dielectric constant of BFO ceramic was found 375 at 1 kHz at 300 K. The dielectric loss was found 0.51 at 100 kHz and 300 K.

Biological potential of fraxin of Cortex fraxini against tumor through literature data analysis

Dinesh K Patel 대한종양외과학회 2022 대한임상종양학회 학술대회지 Vol.2022 No.2

Stimuli-Responsive Graphene Nanohybrids for Biomedical Applications

Patel, Dinesh K.,Seo, Yu-Ri,Lim, Ki-Taek Hindawi 2019 Stem cells international Vol.2019 No.-

<P>Stimuli-responsive materials, also known as smart materials, can change their structure and, consequently, original behavior in response to external or internal stimuli. This is due to the change in the interactions between the various functional groups. Graphene, which is a single layer of carbon atoms with a hexagonal morphology and has excellent physiochemical properties with a high surface area, is frequently used in materials science for various applications. Numerous surface functionalizations are possible for the graphene structure with different functional groups, which can be used to alter the properties of native materials. Graphene-based hybrids exhibit significant improvements in their native properties. Since functionalized graphene contains several reactive groups, the behavior of such hybrid materials can be easily tuned by changing the external conditions, which is very useful in biomedical applications. Enhanced cell proliferation and differentiation of stem cells was reported on the surfaces of graphene-based hybrids with negligible cytotoxicity. In addition, pH or light-induced drug delivery with a controlled release rate was observed for such nanohybrids. Besides, notable improvements in antimicrobial activity were observed for nanohybrids, which demonstrated their potential for biomedical applications. This review describes the physiochemical properties of graphene and graphene-based hybrid materials for stimuli-responsive drug delivery, tissue engineering, and antimicrobial applications.</P>

Nanocellulose assisted hydrogel platforms for improved wound healing and biosensing applications

Dinesh K. Patel,Ki-Taek Lim 강원대학교 산림과학연구소 2022 강원대학교 산림과학연구소 학술대회 Vol.2022 No.10

The development of multifunctional wearable electronic devices has received considerable attention because of their attractive applications. However, integrating multifunctional abilities into one component remains a challenge. To address this, we have developed a tannic acid-functionalized spherical nanocellulose/polyvinyl alcohol composite hydrogel using borax as a crosslinking agent for strain-sensing applications. The hydrogel demonstrates improved mechanical and recovery strengths and maintains its mechanical strength under freezing conditions. The hydrogels show ultra-stretching, adhesive, self-healing, and conductive properties, making them ideal candidates for developing strain-based wearable devices. The hydrogel exhibits good sensitivity with a 4.75 gauge factor at 360% strain. The cytotoxicity of the developed hydrogels is monitored in human dermal fibroblast cells by WST-8 assay in vitro. The antibacterial potential of the hydrogels is evaluated using Escherichia coli. The developed hydrogels demonstrate enhanced antibacterial ability compared with the control. Thus, multifunctional hydrogels with desirable properties are a promising platform for designing strain-based sensor devices.

Synthesis and Characterization of Eggshell-Derived Hydroxyapatite Bioceramics

Dinesh K. Patel,김혜빈,임기택 한국농업기계학회 2019 바이오시스템공학 Vol.44 No.2

Several methods are frequently used to make high-value-added biomaterials from waste biomass for various biomedical applications. Hydroxyapatite (HA), a type of bio-ceramic material, has received considerable attention for use in bone tissue and implanted medical devices owing to its physiochemical properties and excellent biocompatibility. It can be synthesized from calcium- and phosphorous-containing precursor moieties such as calcium oxide and tricalcium phosphate (TCP). In this study, we synthesized an HA bio-ceramic from bio-waste eggshells via a reaction between calcium oxide and TCP, followed by heat treatment. The synthesized HAwas characterized through scanning electron microscopy, X-ray diffraction, and energy-dispersive X-ray spectroscopy. The material exhibited no significant cytotoxicity even at a higher concentration, indicating high biocompatibility. Our study provides an effective way of converting low-cost bio-waste eggshells into a value-added biomaterial for tissue-engineering applications.

Biodegradable 3D-printed self-standing scaffolds of cellulose nanocrystals reinforced chitosan/silk fibroin for improved bone regeneration

Dinesh K. Patel,Ki-Taek Lim 강원대학교 산림과학연구소 2021 강원대학교 산림과학연구소 학술대회 Vol.2021 No.10

3D-printed biodegradable cellulose nanocrystal (CNC)-reinforced chitosan/silk fibroin scaffolds were synthesized as an advanced material for regenerative medicine application. The developed bio-inks were characterized using Fourier transform infrared spectroscopy and X-ray diffraction analysis. The composite scaffolds exhibited better swelling potential and better degradation potential than the pure polymer scaffolds. The cytotoxicity of the developed scaffolds was measured using the WST-1 assay in the presence of human bone marrow-derived mesenchymal stem cells (hMSCs). Improved cell viability and mineral deposition were observed with composite scaffolds vis-à-vis pure polymer scaffolds, showing their improved biocompatibility and mineralization potential. Upregulation of osteogenic associated gene markers was observed in the composite scaffold-treated media compared to the control, indicating enhanced osteogenic efficiency. The osteogenic induction process occurs via the mitogen-activated protein kinase pathway. Higher M1 macrophages polarization occurred in the scaffolds treated media after 24 h of incubation, which assisted the angiogenesis in bone tissue regeneration during the initial stage. Moreover, shifting from M1 to M2 macrophages polarization was observed in the scaffolds treated media after 3 days of incubation, suggesting its tissue regeneration potential. Compared to the control group, enhanced bone regeneration was observed in the calvaria defect rat model with the printed scaffolds, indicating their superior osteogenic efficiency. These results demonstrate that the printed scaffolds are promising materials for vascularized bone-healing applications and provide practical approaches for biomaterial development.

3D-Printed Scaffolds with Reinforced Poly (Lactic Acid)/Carbon Nanotube Filaments Based on Melt Extrusion

김혜빈,Dinesh K. Patel,서유리,임기택 한국농업기계학회 2019 바이오시스템공학 Vol.44 No.2

Personalized medicine suitable for individual patients in tissue engineering is a significant challenge. Owing to the recent growth of 3D printing, various methods of building objects have been proposed. However, there is very little information about the mechanical properties of the pieces obtained by controlling the process variables using composite filaments. Fused deposition modeling (FDM) technology was used to fabricate new scaffolds with infill patterns, interconnected channel networks, controllable porosity, and size. Polylactic acid (PLA)/carbon nanotube (CNT) filaments were synthesized using the melt extrusion technique. An improvement in the mechanical properties was observed in composites compared with the pure polymer. Moreover, no toxicity was expressed by stem cells after 24 h of incubation in the presence of composite filaments for a high CNTconcentration. Our results will aid in the scaffold design of composite filaments through the modeling of process parameters and mechanical properties.

A fully automated bioreactor system for precise control of stem cell proliferation and differentiation

Lim, Ki-Taek,Patel, Dinesh K.,Seonwoo, Hoon,Kim, Jangho,Chung, Jong Hoon Elsevier 2019 Biochemical engineering journal Vol.150 No.-

<P><B>Abstract</B></P> <P>The high proliferation ability and pluripotency of stem cells are ideal for application in stem cell therapy and tissue engineering. However, the clinical use of human mesenchymal stem cells (hMSCs) is limited due to the technical difficulties associated with mass production, high manufacturing costs, and contamination. Bioreactor systems can modulate the environmental and biochemical cues and also induce biomechanical cues, such as shear stress and hypoxia, during mass production of stem cells. In this study, we developed a fully automated bioreactor system (fABS) for precisely controlling the stem cell fate. We used this system for the proliferation and differentiation of human bone derived MSCs (hBMSCs). The fABS mainly consists of five systems: a monitoring system, the primary control system, a medium feeding system, a mass flow controller, and a cell culture system. We evaluated the precision with which the fABS regulated the stem cell environment. We observed that the shear stress induced by the fABS enhanced the hBMSC proliferation and osteogenic differentiation. Moreover, hypoxia induced by the fABS enhanced the chondrogenic differentiation of hBMSCs. As this system can have various applications, such as mass production, osteogenic differentiation, and chondrogenic differentiation, the fABS can aid in advancing the stem cell technologies.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel water-jacketed mobile culture system with perfusion flow for tissue engineering applications. </LI> <LI> Automated control of cell culture conditions. </LI> <LI> Real-time monitoring of cell growth processes <I>via</I> an inverted microscope. </LI> <LI> Significant enhancement in cell growth and osteogenic differentiation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>