http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Nitrendipine, an antihypertensive alpha calcium channel blocker, is cytotoxic to neuroblastoma cells
Isabel Rivera,David M. Cauvi,Nelson Arispe,Antonio De Maio 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.4
Backgrounds: Alpha calcium channel blockers (CCB) are widely prescribed for the treatment of hypertension, including Nitrendipine (NTD). The anti-hypertension effect of NTD is related to binding and blocking the activity of the voltage-gated L-type calcium channels. These channels consist of four subunits (α1, α2/δ, γ and β subunits), localized in the plasma membrane. Methods: Cellular proliferation was determined by the XTT method and apoptosis was measured by flow cytometry. The expression of the α1c subunit (Cacna1c) of the voltage-gated L-type calcium channels was reduced by siRNA and assessed by quantitative RT-PCR. Results: NTD blocked the proliferation of murine neuroblastoma (N2a cells) and induced apoptosis in a concentration- dependent manner. A decrease in Cacna1c expression did not have a significant effect on NTD toxicity of N2a cells. Conclusion: NTD induced apoptosis on neuroblastoma cells, appears to be independent of the expression of the L-type calcium channel.