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RISS 인기검색어
- 검색키워드
- 저자 : Das Premananda (검색결과 2 건)
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Parida, Asish,Das, Anath Bandlhu,Das, Premananda 한국식물학회 2002 Journal of Plant Biology Vol.45 No.1
We studied salt stress-induced biochemical changes in young, hydroponically grown plants of mangrove, Bruguiera parviflora (Rhizophoraceae). Our focus was on the effect of NaCl (applied at 100, 200, 400, or 500 mM) on leaf pigments, total soluble proteins, total free amino acids, carbohydrates, polyphenols, and proline. The total Chi content increased for 14 d after treatment with 100 mM NaCl, then gradually stabilized. At 400 mM, the total Chi content slowly decreased over the 45-d test period. However, the Chi a:b ratio remained unchanged in isolated chloroplasts and in leaf tissue. Percent changes in the carotenoids content followed the same trend as for Chi, except for a 1.5-fold decrease during the 400 -mM NaCl treatment, compared with the control. The total sugar content increased by 2.5- fold by Day 45 after treatment with 400mM NaCl, whereas the starch content measured in the same treatment decreased by 40 to 45%. Leaf protein content decreased as salinity increased, which suggests either a possible disruption in the protein synthesis mechanism or, more likely, an increase in proteolytic activity. The total amino-acid pool increased steadily, by four-fold, in the 45-d, 400-mM treatment Both proline and polyphenols accumulated with increasing levels of salinity, which confirms the role of proline as a stress-induced protective metabolite in the adaptive process of this species. Our results showed that a true mangrove such as B, parviflora can easily be sustained and propagated under low-salinity conditions. At high levels of salinity (∼400 mM, beyond which they could not survive), the plants became adapted to salt stress after two to three weeks. During this adaptive period, changes in pigment and protein levels also occurred. The accumulation of proline and polyphenols played a key role in the plant's stress-induced adjustment to NaCl under hydroponic culture conditions.
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Mattagajasingh Ilwola,Acharya Laxmikanta,Mukherjee Arup Kumar,Das Premananda The Korean Society of Plant Biotechnology 2005 Plant molecular biology and biotechnology research Vol.7 No.2
Buffer soluble protein and five isozymes were analyzed to assess the inter specific relationship among 25 species of the genus Mammillaria Haw. A total of 102 types of proteins were resolved, out of which eighty-six types were found to be polymorphic and only two were unique. A total of 248 bands (isoforms) were detected for 5 isozymes, among them only 4 were found to be monomorphic and 35 were exclusive. Mantel 'Z' statistics revealed wide variations in the correlation among different enzymes. The correlation value 'r' was the highest in case of esterase with pooled data of all the five enzymes. The dendrogram constructed on the basis of pooled data (protein and allozyme) divided the species into two major clusters containing 14 and 11 members respectively. The species M. matudae and M. bella were found to be the most closely related while M. decipience and M. camptroticha were distantly apart. The present study gave an indication of usefulness of the isozyme and protein markers for genetic discrimination between different species of Mammillaria.
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