Distinct Pro-Apoptotic Properties of Zhejiang Saffron against Human Lung Cancer Via a Caspase-8-9-3 Cascade

Liu, Dan-Dan,Ye, Yi-Lu,Zhang, Jing,Xu, Jia-Ni,Qian, Xiao-Dong,Zhang, Qi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Lung cancer is the leading cause of cancer-related death worldwide. Here we investigated the antitumor effect and mechanism of Zhejiang (Huzhou and Jiande) saffron against lung cancer cell lines, A549 and H446. Using high performance liquid chromatography (HPLC), the contents of crocin I and II were determined. In vitro, MTT assay and annexin-V FITC/PI staining showed cell proliferation activity and apoptosis to be changed in a dose- and time-dependent manner. The inhibition effect of Jiande saffron was the strongest. In vivo, when mice were orally administered saffron extracts at dose of 100mg/kg/d for 28 days, xenograft tumor size was reduced, and ELISA and Western blotting analysis of caspase-3, -8 and -9 exhibited stronger expression and activity than in the control. In summary, saffron from Zhejiang has significant antitumor effects in vitro and in vivo through caspase-8-caspase-9-caspase-3 mediated cell apoptosis. It thus appears to have more potential as a therapeutic agent.

Piceatannol Inhibits Phorbol Ester-Induced NF-kappa B Activation and COX-2 Expression in Cultured Human Mammary Epithelial Cells.

Liu, Dan,Kim, Do-Hee,Park, Jong-Min,Na, Hye-Kyung,Surh, Young-Joon Lawrence Erlbaum Associates, Publishers [etc.] 2009 Nutrition and cancer Vol.61 No.6

<P>There are multiple lines of evidence supporting that inflammation is causally linked to carcinogenesis. Abnormal upregulation of cyclooxygenase-2 (COX-2), a rate-limiting enzyme in the prostaglandin biosynthesis, has been implicated in carcinogenesis. Trans-3,4,3',5'-tetrahydroxystilbene (piceatannol), a naturally occurring hydroxylated stilbene with potent anti-inflammatory and antioxidative activities, has been shown to inhibit the proliferation of several cancer cells by inducing apoptosis or blocking cell cycle progression. In this study, we examined the effect of piceatannol on activation of the nuclear transcription factor NF-kappa B, one of the major transcription factors that regulate proinflammatory COX-2 gene transcription, in human mammary epithelial (MCF-10A) cells treated with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). When pretreated to MCF-10A cells, piceatannol markedly inhibited TPA-induced NF-kappa B DNA binding to a greater extent than resveratrol and oxyresveratrol, stilbene analogs structurally related to piceatannol. Piceatannol also inhibited TPA-induced phosphorylation and degradation of Ikappa Balpha as well as nuclear translocation of the phosphorylated form of p65, the functionally active subunit of NF-kappa B. Likewise, TPA-induced expression of COX-2 was abrogated by piceatannol pretreatment. The thiol reducing agent dithiothreitol abolished the inhibitory effects of piceatannol on NF-kappa B DNA binding activity, suggesting that piceatannol may directly modify NF-kappa B or its regulator through reaction with the cysteine thiol(s).</P>

Comment on “preparation and electrorheology of new mesoporous polypyrrole/MCM-41 suspensions”

Liu, Ying Dan,Choi, Hyoung Jin Springer-Verlag 2009 JOURNAL OF MATERIALS SCIENCE - Vol.44 No.11

Selection and identification of single-domain antibody against Peste des Petits Ruminants virus

Dan Liu,Lingxia Li,Xiaoan Cao,Jinyan Wu,Guoyu Du,Youjun Shang 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.4

Background: Peste des petits ruminants (PPR) is an infectious disease caused by the peste des petits ruminants virus (PPRV) that mainly produces respiratory symptoms in affected animals, resulting in great losses in the world's agriculture industry every year. Single-domain variable heavy chain (VHH) antibody fragments, also referred to as nanobodies, have high expression yields and other advantages including ease of purification and high solubility. Objectives: The purpose of this study is to obtain a single-domain antibody with good reactivity and high specificity against PPRV. Methods: A VHH cDNA library was established by immunizing camels with PPRV vaccine, and the capacity and diversity of the library were examined. Four PPRV VHHs were selected, and the biological activity and antigen-binding capacity of the four VHHs were identified by western blot, indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA) analyses. ELISA was used to identify whether the four VHHs were specific for PPRV, and VHH neutralization tests were carried out. ELISA and western blot analyses were used to identify which PPRV protein was targeted by VHH2. Results: The PPRV cDNA library was constructed successfully. The library capacity was greater than 2.0 × 106 cfu/mL, and the inserted fragment size was approximately 400 bp to 2000 bp. The average length of the cDNA library fragment was about 1000 bp, and the recombination rate was approximately 100%. Four single-domain antibody sequences were selected, and proteins expressed in the supernatant were obtained. The four VHHs were shown to have biological activity, close affinity to PPRV, and no cross-reaction with common sheep diseases. All four VHHs had neutralization activity, and VHH2 was specific to the PPRV M protein. Conclusions: The results of this preliminary research of PPRV VHHs showed that four screened VHH antibodies could be useful in future applications. This study provided new materials for inclusion in PPRV research.

RNA m5C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer

Liu Xiaoyi,Wei Qinglv,Yang Chenyue,Zhao Hongyan,Xu Jie,Mobet Youchaou,Luo Qingya,Yang Dan,Zuo Xinzhao,Chen Ningxuan,Yang Yu,Li Li,Wang Wei,Yu Jianhua,Xu Jing,Liu Tao,Yi Ping 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

5-Methylcytosine (m5C) is a common RNA modification that modulates gene expression at the posttranscriptional level, but the crosstalk between m5C RNA modification and biomolecule condensation, as well as transcription factor-mediated transcriptional regulation, in ovarian cancer, is poorly understood. In this study, we revealed that the RNA methyltransferase NSUN2 facilitates mRNA m5C modification and forms a positive feedback regulatory loop with the transcription factor E2F1 in ovarian cancer. Specifically, NSUN2 promotes m5C modification of E2F1 mRNA and increases its stability, and E2F1 binds to the NSUN2 promoter, subsequently reciprocally activating NSUN2 transcription. The RNA binding protein YBX1 functions as the m5C reader and is involved in NSUN2-mediated E2F1 regulation. m5C modification promotes YBX1 phase separation, which upregulates E2F1 expression. In ovarian cancer, NSUN2 and YBX1 are amplified and upregulated, and higher expression of NSUN2 and YBX1 predicts a worse prognosis for ovarian cancer patients. Moreover, E2F1 transcriptionally regulates the expression of the oncogenes MYBL2 and RAD54L, driving ovarian cancer progression. Thus, our study delineates a NSUN2-E2F1-NSUN2 loop regulated by m5C modification in a manner dependent on YBX1 phase separation, and this previously unidentified pathway could be a promising target for ovarian cancer treatment.

The CREG1-FBXO27-LAMP2 axis alleviates diabetic cardiomyopathy by promoting autophagy in cardiomyocytes

Liu Dan,Xing Ruinan,Zhang Quanyu,Tian Xiaoxiang,Qi Yanping,Song Haixu,Liu Yanxia,Yu Haibo,Zhang Xiaolin,Jing Quanmin,Yan Chenghui,Han Yaling 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Autophagy plays an important role in the development of diabetic cardiomyopathy. Cellular repressor of E1A-stimulated genes 1 (CREG1) is an important myocardial protective factor. The aim of this study was to investigate the effects and mechanisms of CREG1 in diabetic cardiomyopathy. Male C57BL/6 J mice, Creg1 transgenic mice and cardiac-specific knockout mice were used to establish a type 2 diabetes model. Small animal ultrasound, Masson’s staining and western blotting were used to evaluate cardiac function, myocardial fibrosis and autophagy. Neonatal mouse cardiomyocytes (NMCMs) were stimulated with palmitate, and the effects of CREG1 on NMCMs autophagy were examined. CREG1 deficiency exacerbated cardiac dysfunction, cardiac hypertrophy and fibrosis in mice with diabetic cardiomyopathy, which was accompanied by exacerbated autophagy dysfunction. CREG1 overexpression improved cardiac function and ameliorated cardiac hypertrophy and fibrosis in diabetic cardiomyopathy by improving autophagy. CREG1 protein expression was decreased in palmitate-induced NMCMs. CREG1 knockdown exacerbated cardiomyocyte hypertrophy and inhibited autophagy. CREG1 overexpression inhibited cardiomyocyte hypertrophy and improved autophagy. LAMP2 overexpression reversed the effect of CREG1 knockdown on palmitate-induced inhibition of cardiomyocyte autophagy. CREG1 inhibited LAMP2 protein degradation by inhibiting the protein expression of F-box protein 27 (FBXO27). Our findings indicate new roles of CREG1 in the development of diabetic cardiomyopathy.

Equilibrium Risk Decision Model for Bidding Electricity Quantity Deviation of Cascade Hydropower Stations

Liu Fang,Xu Yun,Zhu Dan-Dan,Wei Si-Ming,Tang Cheng-Peng 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.2

There is a common phenomenon in China where the upstream and downstream hydropower stations of Cascade Hydropower Stations (CHS) belong to diferent operating entities. When independently participating in market bidding, upstream and downstream hydropower station information sharing blocked is likely to lead to the water fow mismatch of bidding volume, and resulting in no water can be used or water-abandond of downstream power plant. To make up bidding electricity shortfall of downstream station in day-ahead power market, a balance mechanism is proposed, in which downstream station through power contract transaction in the days or system punishment to balance electricity deviation. Power contract transaction includes centralized contract market and negotiated transaction with upstream station, system unbalancing penalty also as virtual transactions, then the problem is switched to electricity deviation optimal allocation in the trading portfolio. For price volatility of all transaction types, trading portfolio risk decision model of downstream power stations deviation electricity based CVaR framework is builded, which achieving minimum CVaR risk of downstream power plant electricity deviation as well as meetting preset expectation loss constraint. Finally, through the analysis of a CHS in southwestern China, it is shown that the model and method proposed in this paper can efectively reduce the imbalance between the winning electricity and incoming water matching of downstream power stations, and efectively improve the stability of hydropower consumption and power market operation

A Measure Model for Synergy Degree between Technology Innovation and Institution Innovation and Its Empirical Analysis

Liu Dan,Yao Ping 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.10

Technological innovation and institutional innovation has a certain relative independence, at the same time, there is a co-evolution relationship between them, which interdependence and mutual promotion. In this paper, from the perspective of co-evolution analysis, Research on the relationship between technological innovation and institutional innovation in the process of resource-based city development, construct a measure model for synergy degree of compound system. A case study of Jixi city in resource based cities, analysis of the collaborative development of technological innovation and institutional innovation.

Microstructure and photoluminescence properties of Nd-doped Bi4Ti3O12 nanocrystalline

Dan Liu 한양대학교 세라믹연구소 2012 Journal of Ceramic Processing Research Vol.13 No.S1

Bi4-xNdxTi3O12 (x = 0.25, 0.5, 0.75, 1.00) nanocrystalline was prepared by modified sol-gel method. The powders crystallized well after annealing at 830 ο C for 30 min in air. Phase composition and optical properties of Bi4-xNdxTi3O12 nanocrystalline were investigated by X-ray diffraction (XRD), scanning electron microscope (SEM), photoluminescence (PL) spectra and ultraviolet-visible (UV-Vis) absorption spectra. In PL spectra, the major up-converted band observed at 534 nm (green) originated from the 4 G7/24 I9/2 excited state absorption (ESA) when exciting at 800 nm. It was also found that with increasing of x value the luminous intensity decreased because of different location of Nd3+ substitution. The optical energy band gap of the Bi3.5Nd0.5Ti3O12 nanocrystalline was found to be 3.17 eV by Tauc equation. The up-conversion mechanisms in the doped system will be discussed by analyzing the energy level structures of Nd3+.

Deep removal of sulfur from real diesel by catalytic oxidation with halogen-free ionic liquid

Dan Liu,Jianzhou Gui,Yong-Ki Park,Shuang Yang,Yuhuan Gao,Xilai Peng,Zhaolin Sun 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.1

A halogen-free task-specific ionic liquid (TSIL) of 1-(2-carboxylic acid) ethyl-3-methylimidazolium bisulfate ([(CH2)2COOHmim] [HSO4]) is applied for deep oxidative desulfurization of real diesel as a catalyst and reaction media. The sulfur level of real diesel can be reduced from 200 to 20.5 μg/g at room temperature with small amount of ionic liquid using H2O2 as an oxidant. The used ionic liquid can be easily recycled and no obvious decrease in catalytic activity can be found after recycling five times.