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RISS 인기검색어
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- 저자 : Daeho Jang(장대호) (검색결과 2 건)
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Particle size-dependent Marangoni vortex phenomenon in the evaporating droplet system
Changdeok Seo(서창덕),Daeho Jang(장대호),Jongjin Chae(채종진),Sehyun Shin(신세현) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11
Dissolving surfactant in water causes a vortex flow near droplet edge and leads to uniform patterns of colloidal particle deposition after evaporation. Radially-outward capillary flow and radially-inward Marangoni flow drive this vortex flow. Recently, Still, T. et al discovered that the motions of particles are affected by initial concentrations of surfactant (Sodium dodecyl sulfate). In this paper, we observed that particle behaviors are also affected by the particle size. 3 different sizes of PS (polystyrene) particle samples had different behavior in a fixed initial concentration of surfactant (SDS) solution. 3㎛ particles rotated slowly and 1μm particles rotated quickly. However 5㎛ particles were not rotating. Therefore, we suggest that not only the initial concentration but also the suspended particle size should be considered an important factor of the vortex flow phenomenon. In addition, the behaviors of particles in an evaporating droplet system could be controlled by adjusting the initial concentration of surfactant and the particle size.
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표면 플라스몬 공명을 이용한 TP53의 점돌연변이 검출
박세라(Sera Park),장대호(Daeho Jang),신세현(Sehyun Shin) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11
TP53, a type of tumor suppressor gene, regulates other mutations involving numerous cancers which mean that its mutation causes fatal disease to human. Thus, sensitive detection technique of this gene is necessary for early diagnosis and targeted therapy. In this study, we developed the detection of TP53 of one-point mutation using surface plasmon resonance (SPR). We immobilized the complementary DNA of wild type TP53 as a receptor on bare gold chip surface which is attached thiol functional group (Probe; HS-C6-26mer). Wild type DNA and one-point mutated DNA are injected into two different micro channels formed on a single gold sensor chip simultaneously. Because the affinity between receptor DNA and wild type DNA is greater than it of receptor and mutation DNA, the SPR signal from wild type DNA channel was three-times higher than one-point mutated DNA. As a result, our sensor is able to distinguish remarkably point mutation with wild type of the gene. Accordingly, this technique will be a powerful biosensor for detection of the mutation and helpful to preventing metastasis of cancer.
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