Analysis of Matrix Metalloproteinase Activation of Two Major Embryo Culture Medium in Embryo Development

Da-Hye Shin,Na-Hyeon Jung,Ji-Hye Lee,Sang-Hwan Kim,Jong-Taek Yoon 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2

Present study, it is demonstrated that MMP-9 serum as well as follicular concentrations are related to a successful IVF resulting in pregnancy. Currently, a limited number of studies have investigated intrafollicular and serum MMPs, with inconsistent conclusions. However, MMPs theoretically could be important players in IVF processes. Moreover, different behaviors of MMP-2 and MMP-9 during the embryo development were confirmed; only MMP-9 has shown a vast difference between follicular and maturation oocyts. The main objective of this study is to examine the mode of MMPs activation during bovine in vitro fertilization. In particular, the relative employment of two major embryo culture medium in embryo development; e.g. ES(non-bovine serum) and CR(bovine serum) was compared. This results found that the level of MMP-2 and MMP-9, which degrades basal membrane, was different in the ES and CR in cultured at each stage of embryo development. In ES cultured system, MMP-9 was highly expressed throughout blastocyst inner cell mass and exhibited strong gelatinase activity. The MMP-9 expression continually increased as the embryo develops from oocyte maturation to fertilization, Meanwhile, the level of MMP-2 remained insignificant throughout these periods. However, decreased MMP-2 and 9 expression on the CR culture system. Interestingly, MMP-9 was actively expressed in ES culture system, inner cell mass is showing levels higher than trophoblast cell. Our result suggests a possibility that different subtype of MMP partakes major functions in the remodeling of inner cell mass in blastocyst. Such differences in MMP expression may impact to the distinct mode of follicular development in the two species.

Expression Phenotypic-Genotypic Associations of Tyrosinase SNP with Changing Profiles in Korean Native Chicken Plumage Pigmentation.

Da-Hye Shin,Hye-Mi Park,Chan-Seong Jeong,Sang-Hwan Kim 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10

The tyrosinase (TYR) genes have been accepted as major genes involved in the plumage pigmentation of chickens. Tyrosinase (TYR) gene is located on chromosome 1 in chicken and it is composed of five exons and four introns. TYR gene is described as a key enzyme in melanin biosynthesis. Especially, most examples of color patterns in chicken have been due to differential in the tyrosinase gene. This study was conducted to the association of feather color and sequence polymorphism in the Tyrosinase(TYR) gene was investigated using Korean native chickens(red plumage, red-line plumage, Ogol = KNC) and white leghorn(WL). From WL and KNC breed analyses, 232 differential SNPs were detected in 4th exon and 4th intron of TYR gene respectively. The genotype frequencies for 50 SNPs were compared between KCR, KCRD and KCO represented homozygous SNP types in all the analyzed SNP positions while KNC displayed various SNP types. In this study, we conclude that the variation of a wild type sequence in intron 4 of the tyrosinase gene is pigmentation of the original native chickens in korean. This work was supported by a grant from the “Livestock Preservation of Genetic Resources", Rural Development Administration, Republic of Korea.

Non-glycosidic iridoids from the roots of Patrinia scabra and their nitric oxide production inhibitory effects

Da Hye Lee,Ji-Sun Shin,Jin Su Lee,Shin-Young Kang,Hee-Soo Han,Seung Mok Ryu,Kyung-Tae Lee,Dongho Lee,Dae Sik Jang 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.9

Phytochemical investigation on the 70% aqueousEtOH extract from the roots of Patrinia scabra led tothe isolation and characterization of five new non-glycosidiciridoids, patriscabrins F–J (1–5), along with a knowniridoid 11-ethoxyviburtinal (6). The structures of the newcompounds 1–5 were determined by interpretation ofspectroscopic data, particularly by 1D- and 2D-NMR andECD studies. Thereafter, the isolates 1–6 were evaluatedfor their inhibitory effects on lipopolysaccharide-inducednitric oxide production in RAW 264.7 cells. Of these,patriscabrin F (1) exhibited the most potent inhibitoryeffect with observed IC50 value of 14.1 lM. In addition,patriscabrin G (2) and 11-ethoxyviburtinal (6) showed IC50values 24.6 and 35.5 lM, respectively.

Analysis of Physiological Associative Gene during Estrous Cycle in Porcine Uterine Tissue

Da-Hye Shin,Ji-Hye Lee,Sang-Hwan Kim,Jong-Taek Yoon 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10

The uterus changes according to the estrous cycle and programmed cell death suitable for placenta formation plays a role in implantation and embryo formation. In the present study of this experiment was to understand the programmed cell death(PCD) for the estrus day 15 of uterus in normal and miniature pig. The our results there suevival associated-Gene(mTOR) expression was significantly higher from miniature pigs, but expression of genes related to cellular metabolic activity (IGF, PCNA) was mostly significantly higher in normal pig than the miniature pigs. Expression of the hormone receptor genes (FSH, LH) and 20a-HSD was higher in the normal pig than in the miniature pig. And gene expression of the progesterone receptor was lower in the normal pig. Expression of autophagy-related genes (ATG1, ATG5, ATG13, Beclin- 1) was significantly lower in the uterine tissues of the normal pig than in the miniature pigs, but the expression of the MAP1LC3A gene was significantly higher in the normal pig.There was non-differential in the expression of BAX gene related to apoptosis, but expression of casp-3 gene was significantly higher in normal pig uterine tissue. Our result is suggest that the uterus of normal pig on the 15th day to have high expression of genes involved in cell metabolism activity and cell reorganization as affected by LH hormone. The expression of the genes involved in cellular metabolism and cellular remodeling in the miniature pig uterus tissues seems to be low due to the activity of the progesterone hormone mechanism. The LH and FSH hormones been shown that induce cell expression and metabolic activity of the program cell daeth gene.

Activation of Matrix Metalloproteinases to induce expression of Morphological Changes of Uterus from Endometrium in Hormone Treatment Female Mice

Da-Hye Shin,Sang-Hwan Kim,Seog-Gyu Bang,Chan-Hee Park,Jong-Taek Yoon 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

Our study has analyzed whether inappropriate gonadotropin secretion affects the morphological changes due to the activation of intrauterine MMP. Methods A total of each 6 mice were injected with PMSG, Progesterone, and Androgen in 5 IU of intraperitoneal injection every 2 days after estrus synchronization, and morphological and MMPs expression patterns were compared after inducing hormone secretion. Also, cell survival and death related genes were compared and analyzed. The endometrium was highly developed in the PMSG, and the androgen was not developed at all. In particular, the diameter of the uterus of the Androgen group was also very narrow. MMPs activity assay in the case of PMSG was confirmed that showed low activity, whereas, progesterone and androgen In showed high activity and, in particular, very high activity of MMPs in the case of androgen in glandular cell. The expression of VEGF in the tissues of each group was different from that of MMPs. In the PMSG group, the activity of VEGF was increased in both the Myo-metrium and the endo-metrium, whereas the progesterone group showed low overall expression in the endo-metrium. Therefore, the present study showed that the activities of the endo-metrial cells and the restructuring of the endometrial cells differed according to the type of the abnormal secretory hormone. In particular, the secretion of androgen increased the activity of MMPs throughout the uterus, The endo-metrial epithelial cells are affected by the progesterone group. In conclusion, this study suggests that inappropriate gonadotropin secretion increases the functional changes of the uterus and this reconstruction may be caused by increased activity of MMPs

Expression Phenotypic-Genotypic Associations of Tyrosinase SNP with Changing Profiles in Korean Native Chicken Plumage Pigmentation

Da-Hye Shin,Hye-Mi Park,Chan-Seong Jeong,Sang-Hwan Kim 한국동물생명공학회(구 한국동물번식학회) 2016 발생공학 국제심포지엄 및 학술대회 Vol.2016 No.10

MARK and Wnt Pathway Associated Genes Analysis for the Differential Expression Patterns of the Brindle and Yellow Coat Color Type in Korean Cattle

Da-Hye Shin,Ji-Hye Lee,Sang-Hwan Kim,Ji-Yeon Kim,Jong-Taek Yoon 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05

The coat color of mammals is determined by the melanogenesis pathway, which is responsible for maintaining the balance between black-brown eumelanin and yellow-reddish pheomelanin. The melanogenesis-associated genes controlling pigmentation act as a complex and interact with each other to cause phenotypic and genotypic variations in cattle. That the MC1R genotype of Korean native cattle with dark muzzle was e/e or E+/e, while the genotype of Korean native cattle with light muzzle was E+/E+, which is a variant of the MC1R genotype in the Korean native cattle. Especially, the MC1R expression type is shows how much pigmentation, important factor in deciding its status in the coat and nose colours. However, information regarding the coat or nose colours-associated gene regulation of korean cattle is not yet unknown. Therefore, in this study was to investigate the expression patterns of melanogenesis-associated genes in black dot nose(korea brindle cattle) and normal nose(korea native cattle). Using microarray clustering and real-time polymerase chain reaction techniques, we analysed that the expression of genes involved in the mitogen-activated protein kinase (MAPK) and Wnt signaling pathways is distinctively regulated in the dark and light muzzle tissues. Differential expression of tyrosinase was also noticed, although the difference was not as distinct as those of MAPK and Wnt. We hypothesize that emphasis on the MAPK pathway in the Korea brindle cattle induces eumelanin synthesis through the activation of cAMP response elementbinding protein and tyrosinase, while activation of Wnt signaling counteracts this process and raises the amount of pheomelanin in the native cattle. Regarding the increasing interest in the genetic diversity of cattle stocks, genes we identified for differential expression in the brindle cattle vs. native cattle may serve as novel markers for genetic diversity among cows based on the coat and muzzle color phenotype.

유당분해율에 따른 저지방 유당분해우유의 품질 특성

정다혜 ( Da Hye Jeong ),신용국 ( Yong Kook Shin ),강신호 ( Shin Ho Kang ) 한국유가공기술과학회 2017 Journal of Dairy Science and Biotechnology (JMSB) Vol.35 No.1

본 연구에서는 유당분해율에 따른 저지방우유의 이화학적 특성을 비교해 보고자 하였으며, 유당 함량과 빙점 값의 상관 공식을 도출하여 유당분해 제조공정상 유당분해 정도를 쉽고 빠르게 예측하고자 하였다. 우유의 빙점은 특히 유당 함량과 염류와 같은 미네랄의 변화에 따라 큰 영향을 받는다고 알려져 있는데, 본 실험에서도 10℃에서 시간이 경과함에 따라 유당이 서서히 분해되고 빙점 값도 함께 떨어짐을 확인하였다. 따라서, y=-50.416x+767.91(R²=0.9866, x값은 유당함량(g/100 mL), y값은 빙점 값(m℃))과 같은 상관식을 도출하였다. 단, 유당분해우유의 제조공정, 분해조건, 원유의 특성에 따라 다소 차이는 있을 수 있으나, 공정 표준화된 제품에는 쉽게 적용할 수 있을 것으로 보인다. 유당분해우유는 살균 방법 또는 장기간 저장 중에 마일라드 반응, 갈변화, 풍미 등에 영향을 받는다고 알려져 있으나, 이화학적 특성 분석 결과에서 유당분해율에 따른 샘플 간의 우유 단백질, 유지방, 무지유 고형분, 총 고형분, pH, 산도 값에 유의 차는 없었다(p<0.05). 또한, 색도값과 입자 크기에서도 유당분해율에 따른 샘플 간 다소 차이는 있으나, 뚜렷한 경향은 보이지 않았다. 단, 유지방 함량에 따른 입자크기는 유의적인 차이를 보였다(p<0.05). 본 연구에서는 유당분해율에 따른 저지방우유의 품질 특성을 파악할 때, 장기간 보관 중에 따른 이화학적 변화를 살펴보지 않았다는데 한계가 있다. 따라서, 향후 유당분해우유 품질에 영향을 줄 수 있는 요인인 저장 기간을 달리한 이화학적 특성을 살펴 제품에 다양하게 적용할 수 있는 방안을 모색하면 좋을 것으로 사료된다. The market for lactose-hydrolyzed milk is growing due to an increased awareness of lactose intolerance, and increased market interest for modified milk with health claims. The aim of this study was to compare the quality characteristics of 2% lactose-hydrolyzed milk with its lactose hydrolysis rate, with the goal of applying the method to dairy product monitoring in the future. We observed that the freezing point of milk significantly decreased with increasing lactose hydrolysis rate. A linear relationship was found between lactose concentration and freezing point, following the equation (y=-50.416x + 767.91). However, no significant changes were observed in other physicochemical properties of the milk (pH, titratable acidity, total solids and color) (p<0.05). In conclusion, we could predict the residual lactose content quickly and easily in 2% low fat milk by measuring its freezing point. This could represent an easy means for assessing the lactose hydrolysis rate of dairy products.

Iridoids from the Roots of <i>Patrinia scabra</i> and Their Inhibitory Potential on LPS-Induced Nitric Oxide Production

Lee, Da Hye,Shin, Ji-Sun,Kang, Shin-Young,Lee, Seung-Bin,Lee, Jin Su,Ryu, Seung Mok,Lee, Kyung Tae,Lee, Dongho,Jang, Dae Sik American Chemical Society and American Society of 2018 Journal of natural products Vol.81 No.6

<P>An activity-guided fractionation procedure of the 70% aqueous EtOH extract from the roots of <I>Patrinia scabra</I> led to the isolation and characterization of five new iridoids, patriscabrins A-E (<B>1</B>-<B>5</B>), along with 13 known compounds. The structures of <B>1</B>-<B>5</B> were determined by interpretation of spectroscopic data, particularly by 1D and 2D NMR, ECD, and VCD studies. Thereafter, isolates were evaluated for their inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells. Of these, the new iridoids <B>2</B> and <B>5</B> and the known lignan patrineolignan B (<B>6</B>) exhibited IC<SUB>50</SUB> values of 14.7 to 17.8 μM.</P> [FIG OMISSION]</BR>

새로운 감성의 홈퍼니싱 문구 브랜드(MELDEN) 기획 및 제품 시리즈 제안

신다혜 ( Da-hye Shin ) 숙명여자대학교 디자인연구소 2019 숙명디자인학 연구 Vol.27 No.-