肉鷄 飼料添加劑로서 柴胡莖葉의 利用

趙成九,崔香順,朴相一 충북대학교 농업과학기술연구소 2000 農業科學硏究 Vol.17 No.-

Diets containing 0.0%, 0.2%, 0.5%, and 1.0% of SLPBFL were fed to broiler chickens at 7 weeks age. The results of SLPBFL feeding are as follows; Body weight gain was significantly improved in 0.5% SLPBFL diet group ( p < 0.01 ). Because feed consumption was increased with adding of SLPBFL, broilers taste was not considered in this experiment. Feed requirement was analyzed same score to 1.99 in 0.2% and 0.5% SLPBFL added rations be improved about 8% than the control group. The amounts of carcass, drumsticks and breast meat were significantly(p<0.05) plenty in 0.5% treatment, and also the carcass ratio was enhanced to the highest score in 0.5% SLPBFL, In 0.5% treatment, birds were improved of growth rate, the head and neck weights, leg and shank weight, and amounts of blood and feather were higher appeared than other treatments. The weight of internal organs (liver, heart, spleen, gizzard) were appeared to be heavy in 0.5% treatment. The fat accumulation (abdominal and gizzard surrounding) was observed from the broiler chickens fed 0.5%, tended to increase with fat contents agreeably to live weight gain (P<0.05). Total serum protein, serum bilirubin and LDL-cholesterol concentrations in non- SLPBFL were lower than those in SLPBFL treatments. The concentrations of serum GPT, serum GOT, serum albumin and HDL- cholesterol in non-SLPBFL bird were the lowest in treatment. The concentrations of serum HDL-cholesterol and albumin in 0.5% treatment to be were appeared to be highest score and the concentration HDL-cholesterol was analyzed the lowest score, and total serum protein, serum GPT, serum GOT, serum bilirubin, total serum cholesterol, HDL-cholesterol and serum triglyceride concentrations were analyzed to be mean score in experimental diets. Key words : Bupleurum falcatum Linne, serum protein, serum GPT, serum GOT, serum Bilirubin, total serum cholesterol, HDL-cholesterol, serum triglyceride.

當歸莖葉의 肉鷄 飼料添加劑 利用

趙成九,崔香順,朴相一 충북대학교 농업과학기술연구소 2000 農業科學硏究 Vol.17 No.-

Studies on the utilization added in diet with a Stem and Leaf Powder of Angelicae Gigas Nakai(SLPAGN) in the broiler chickens. Diets added with 0.0%, 0.2%, 0.5%, and 1.0% of SLPAGN were fed to broiler chickens at 7 weeks age. The results of SLPAGN feeding are as follows; The body weight gain was significantly improved in 1.0% SLPAGN diet (P<0.01), and feed efficiency was effected with 1.0% SLPAGN ration(P<0.05). The amount of carcass was the heaviest in 0.2% SLPAGN diet. The amounts of breast meat and drumsticks product were the highest in 0.2% SLPAGN, and the weights of head and neck, leg and shank were lower than other treatments. The fat accumulation (abdominal and gizzard surrounding) was observed in broilers fed diets added with 0.2% SLPAGN. The weights of liver, spleen and gizzard were tended to increase with adding SLPAGN rations than control diet. The degradation of fabricius sac was delayed to non-SLPAGN treatment than SLPAGN groups. The blood contents were analyzed to be the highest level to the total serum, GOT, total serum cholesterol and LDL-cholesterol concentrations in the broiler chickens fed 1.0% SLPAGN diets, and then the level of total serum protein and HDL-cholesterol concentrations were appeared to be the lowest. In the birds fed non-SLPAGN treatments the concentration of serum GPT, serum albumin, total serum triglyceride, total serum cholesterol and LDL-cholesterol were analyzed to be the lowest levels. In 0.2% SLPAGN treatment, the contents of serum GOT and serum bilirubin contents were analyzed the lowest levels. Key words: Angelicae Gigas, feed efficiency. HDL-cholesterol. Carcass ratio, serum bilirubin, GPT, serum albumin, serum triglyceride, serum albumin, GOT

표피성장인자(EGF)가 돼지 난자의 성숙과정동안 세포골격에 미치는 영향

김나희, 이아림, 최향순, Qinyue Lu, Zhi Chen, 이송희 충북대학교 농업과학기술연구소 2023 農業科學硏究 Vol.39 No.1

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Gene Expression of Cox5a, 5b, or 6b1 and Their Roles inPreimplantation Mouse Embryos1

Cui, Xiang-Shun,Li, Xing-Yu,Jeong, Yu-Jeong,Jun, Jin-Hyun,Kim,Nam-Hyung Society for the Study of Reproduction 2006 BIOLOGY OF REPRODUCTION Vol.74 No.3

To investigate the role of nuclear encoded genes in mitochondrialfunction during oocyte maturation and early embryogenesis weexamined the expression pattern and function of the cytochromeoxidase (Cox) subunits, Cox5a, 5b, and 6b1 during oocytematuration and early embryo development. Transcription of Cox5a,5b, or 6b1 was observed in oocytes and during early development;their expression levels were abundant in mature oocytes (MII) andzygotes (1C), and lowest at the 2-cell stage (2C), graduallyincreasing from 4-cell to blastocyst stage. Immunocytochemicalstudies revealed that COX5A, 5B, or 6B1 proteins were expressed inall blastomeres of the blastocyst. Silencing of mRNA expression byRNA interference (siRNA) did not inhibit oocyte maturation ordevelopmental events up to the morula and blastocyst stages, butdisrupted mitochondrial distribution. Significantly higherapoptosis and lower cell numbers were observed in siRNA-treatedblastocysts. Real time RT-PCR revealed that silencing of Cox5a,5b, or 6b1 did not alter mRNA levels of Bcl-xL (Bcl2l1), butincreased transcription levels of proapoptotic genes, Bax andcaspase 3 (Casp3). Furthermore, mRNA and protein levels ofE-cadherin (CDH1) were decreased in siRNA microinjectedblastocysts. These results suggest that gene expression of the Coxsubunits, Cox5a, 5b, and 6b1 is not required for embryodevelopmental events up to the blastocyst stage. The loss of thesegenes leads to mitochondrial dysfunction that results in apoptosisof the blastocyst stage embryos.

Identification of differentially expressed genes in murine embryos at the blastocyst stage using annealing control primer system

Cui, Xiang-Shun,Shin, Mi-Ra,Lee, Kyung-Ah,Kim, Nam-Hyung Wiley Subscription Services, Inc., A Wiley Company 2005 Molecular reproduction and development Vol.70 No.3

<P>The identification of embryo-specific genes would provide insights into early embryonic development. However, the current methods employed to identify the genes that are expressed at a specific developmental stage are labor intensive and suffer from high rates of false positives. Here we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) method that involves annealing control primers (ACPs) to identify the genes that are specifically or prominently expressed in mouse blastocysts compared to 4-cell stage embryos. Using 120 ACPs, we identified and sequenced 74 of these differentially expressed genes (DEGs). Basic Local Alignment Search Tool (BLAST) searches revealed that 53 were known genes, 9 encoded ribosomal proteins, and 12 were unknown genes. Of the known genes, 14 were selected and further characterized using real-time quantitative PCR to assess their stage-specific expression in mouse embryos. This analysis suggests that the ACP system is a very good method for the identification of stage-specific genes in small numbers of mouse embryos. Further analysis of the differentially expressed blastocyst genes we have identified will provide insights into the molecular basis of preimplantation development. Mol. Reprod. Dev. 70: 278–287, 2005. © 2005 Wiley-Liss, Inc.</P>

The Role of microRNAs on the Epigenetic Regulation of Fertilized and Cloned Embryo Development

Xiang-Shun Cui,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Cloning or somatic cell nuclear transfer (SCNT) using adult somatic cell to derive cloned embryos is a promising new technology with potential applications in both agriculture and regenerative medicine. Mammalian embryos derived by nuclear transfer are capable of development to the blastocyst stage with a relatively high efficiency of 30~ 50%. However, in full-time development, usually only 2% of NT embryos can result in live births due to abnormalities in placenta formation. In SCNT embryos, the donor cell nucleus is epigenetically reprogrammed by oocyte cytoplasm during development. Incomplete reprogramming of the donor cell genome is considered a major reason for low cloning efficiency. Aberrant epigenetic modifications include DNA methylation, histone modification and X-chromosome-inactivation. Due to a lack of basic knowledge regarding the embryos following nuclear transfer, the success rate of cloning is low. Therefore, elucidation of the molecular mechanism of SCNT embryo development will be of great value for further research. MicroRNAs (microRNA) are single-strand RNA molecules of about 19 23 nucleotides in length, which regulate gene expression by imperfect base pairing with target mRNA, subsequently guiding mRNA cleavage or translational repression. Since the first discovery and functional annotation in 1993 of the small RNA, lin-4 and let-7, which are involved in developmental timing and gene regulation during C. elegans larval development, microRNAs have received scientific attention. Now hundreds of microRNAs have been identified in various multicellular organisms, and many microRNAs have been shown to be evolutionarily conserved. The roles proposed for this novel class of tiny RNA molecules are diverse. They are likely to be involved in developmental timing, differentiation, cell proliferation, signaling pathways, apoptosis, metabolism, heterochromatin formation, genome rearrangement, brain development and carcinogenesis. Currently (2006- present) we are working to determine the role of microRNAs on the epigenetic regulation of fertilized and cloned embryo development. The general hypothesis of our research is that genetic and epigenetic factors regulate the development of preimplantation mammalian embryos, and aberrant modulations in cloned embryos are causes of abnormal development and low success rate of cloned embryos.

Maternal Gene Transcription in Mouse Oocytes: Genes Implicated in Oocyte Maturation and Fertilization

CUI, Xiang-Shun,LI, Xing-Yu,YIN, Xi-Jun,KONG, IL Keun,KANG, Jason-Jongho,KIM, Nam-Hyung 家畜繁殖硏究所 2007 Journal of Reproduction and Development Vol.53 No.2

<P>Maternal gene expression is an important biological process in oocyte maturation and early cleavage. To gain insights into oocyte maturation and early embryo development, we used microarray analysis to compare the gene expression profiles of germinal vesicle (GV)- and metaphase II (MII)-stage oocytes. The differences in spot intensities were normalized and grouped using the Avadis Prophetic software platform. Of the 12164 genes examined, we found 1682 genes with more highly expression in GV-stage oocytes than in MII-stage oocytes, while 1936 genes were more highly expressed in MII-stage oocytes (P<0.05). The genes were grouped on the basis of the Panther classification system according to their involvement in particular biological processes. The genes that were up-regulated in GV oocytes were more likely to be involved in protein metabolism and modification, the mitotic cell cycle, electron transport, or fertilization or belong to the microtubule/cytoskeletal protein family. The genes specifically upregulated in the MII oocytes were more likely to be involved in DNA replication, amino acid metabolism, or expression of G protein-coupled receptors and signaling molecules. Identification of genes that are preferentially expressed at particular oocyte maturation stages provides insights into the complex gene regulatory networks that drive oocyte maturation and fertilization.</P>

Maternally derived transcripts: identification and characterisation during oocyte maturation and early cleavage

Cui, Xiang-Shun,Kim, Nam-Hyung CSIRO Publishing 2007 Reproduction, fertility, and development Vol.19 No.1

<P> The identification and characterisation of differentially regulated genes in oocytes and early embryos are required to understand the mechanisms involved in maturation, fertilisation, early cleavage and even long-term development. Several methods, including reverse transcription-polymerase chain reaction-based suppression subtractive hybridisation, differential display and cDNA microarray, have been applied to identify maternally derived genes in mammalian oocytes. However, conventional gene-knockout experiments to determine specific gene functions are labour intensive and inefficient. Recent developments include the use of RNA interference techniques to establish specific gene functions in mammalian oocytes and early embryos. Regulation of the poly(A) tail length is a major factor in controlling the activities of maternal transcripts in mammals. Further studies are required to clarify the mechanisms by which expression levels of maternally derived transcripts are regulated. In the present review, we focus on the identification and functions of the differentially expressed transcripts during oocyte maturation, fertilisation and early cleavage. </P>

Polyamine Prevent Apoptotic Cell Death by Regu1ation of Apoptosis Related Gene Expression in Porcine Parthenotes

Xiang-Shun Cui,Yong-Xun Jin,Kyu-Chan Hwang,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2004 Reproductive & Developmental Biology(Supplement) Vol.28 No.2s

Mouse granulocyte-macrophage colony-stimulating factor enhances viability of porcine embryos in defined culture conditions

Cui, Xiang-Shun,Lee, Jae Yeong,Choi, Seok Hwa,Kwon, Mo Sun,Kim, Teoan,Kim, Nam-Hyung 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

The aim of this study was to determine the effects of mouse granulocyte-macrophage colony-stimulating factor (mGM-CSF) on development of porcine parthenotes and nuclear transferred embryos, and on their expression of implantation-related genes. In the presence of bovine serum albumin, mGM-CSF did not increase the percentage of oocytes that developed to the blastocyst stage and at day 7 did not increase cell numbers of embryos. Addition of 2 ng/ml GM-CSF to protein-free culture medium significantly increased the compaction and blastocoel formation of 1-to 2-cell parthenotes developing in vitro. However, total cell numbers were not increased when they were cultured in the presence of GM-CSF. Semi-quantitative reverse transcriptase polymerase chain reaction revealed that mGM-CSF enhances mRNA expression of the leukemia inhibitory factor receptor, but does not influence interleukin-6 or sodium/glucose co-transporter protein gene expression in blastocyst state parthenotes. These results suggest that mGM-CSF may enhance viability of porcine embryos developing in vitro in a defined culture medium.