Preserving the inflated structure of lyophilized sporopollenin exine capsules with polyethylene glycol osmolyte

Corliss, Michael K.,Bok, Chuan Kiat,Gillissen, Jurriaan,Potroz, Michael G.,Jung, Haram,Tan, Ee-Lin,Mundargi, Raghavendra C.,Cho, Nam-Joon THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING 2018 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY -S Vol.61 No.-

<P><B>Abstract</B></P> <P>Extracted from natural pollen grains, sporopollenin exine capsules (SECs) are robust, chemically inert biopolymer shells that posess highly uniform size and shape characteristics and that can be utilized as hollow microcapsules for drug delivery applications. However, it is challenging to extract fully functional SECs from many pollen species because pollen grains often collapse, causing the loss of architectural features, loading volume, and bulk uniformity. Herein, we demonstrate that polyethylene glycol (PEG) osmolyte solutions can help preserve the native architectural features of extracted SECs, yielding inflated microcapsules of high uniformity that persist even after subsequent lyophilization. Optimal conditions were first identified to extract SECs from cattail (<I>Typhae angustfolia</I>) pollen <I>via</I> phosphoric acid processing after which successful protein removal was confirmed by elemental (CHN), mass spectrometry (MALDI-TOF), and confocal laser canning microscopy (CLSM) analyses. The shape of SECs was then assessed by scanning electron microscopy (SEM) and dynamic image particle analysis (DIPA). While acid-processed SECs experienced high degrees of structural collapse, incubation in 2.5% or higher PEG solutions significantly improved preservation of spherical SEC shape by inducing inflation within the microcapsules. A theoretical model of PEG-induced osmotic pressure effects was used to interpret the experimental data, and the results show excellent agreement with the known mechanical properties of pollen exine walls. Taken together, these findings demonstrate that PEG osmolyte is a useful additive for preserving particle shape in lyophilized SEC formulations, opening the door to broadly applicable strategies for stabilizing the structure of hollow microcapsules.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Identified that polyethylene glycol (PEG) osmolyte can prevent SEC particle collapse. </LI> <LI> Chemical route to extract SECs from cattail pollen was achieved successfully. </LI> <LI> Model of PEG-induced osmotic pressure effects agrees with experimental data. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Preserving the inflated structure of lyophilized sporopollenin exine capsules with polyethylene glycol osmolyte

Michael K. Corliss,Chuan Kiat Bok,Jurriaan Gillissen,Michael G. Potroz,정하람,Ee-Lin Tan,Raghavendra C. Mundargi,조남준 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.61 No.-

Extracted from natural pollen grains, sporopollenin exine capsules (SECs) are robust, chemically inert biopolymer shells that posess highly uniform size and shape characteristics and that can be utilized as hollow microcapsules for drug delivery applications. However, it is challenging to extract fully functional SECs from many pollen species because pollen grains often collapse, causing the loss of architectural features, loading volume, and bulk uniformity. Herein, we demonstrate that polyethylene glycol (PEG) osmolyte solutions can help preserve the native architectural features of extracted SECs, yielding inflated microcapsules of high uniformity that persist even after subsequent lyophilization. Optimal conditions were first identified to extract SECs from cattail (Typhae angustfolia) pollen via phosphoric acid processing after which successful protein removal was confirmed by elemental (CHN), mass spectrometry (MALDI-TOF), and confocal laser canning microscopy (CLSM) analyses. The shape of SECs was then assessed by scanning electron microscopy (SEM) and dynamic image particle analysis (DIPA). While acid-processed SECs experienced high degrees of structural collapse, incubation in 2.5% or higher PEG solutions significantly improved preservation of spherical SEC shape by inducing inflation within the microcapsules. A theoretical model of PEG-induced osmotic pressure effects was used to interpret the experimental data, and the results show excellent agreement with the known mechanical properties of pollen exine walls. Taken together, these findings demonstrate that PEG osmolyte is a useful additive for preserving particle shape in lyophilized SEC formulations, opening the door to broadly applicable strategies for stabilizing the structure of hollow microcapsules.

Effects of smoking and marination on the sensory characteristics of cold-cut chicken breast filets: A pilot study

Shilpa S. Samant,Philip G. Crandall,Corliss A. O’Bryan,Jody M. Lingbeck,Elizabeth M. Martin,Tonya Tokar,서한석 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6

This study aimed to determine individual and combined effects of smoking and marination on the sensory characteristics of boneless, skinless chicken breast meat. Four types of cooked, cold-cut chicken breast meat, i.e., marinated cooked, marinated smoked, and controls of non-marinated cooked and non-marinated smoked chicken, were evaluated for 28 sensory characteristics. Marination significantly increased saltiness, sweetness, roasted flavor, smoked flavor, and moistness of the cold-cut chicken breast meat. In addition, smoking significantly enhanced the saltiness, bitterness, roasted flavor, smoked flavor, and moistness of mass. Interestingly, a combination of smoking and marination processes resulted in a synergistic increase in the perceived moistness of mass compared to their individual treatments. In conclusion, this study demonstrates individual and combined influences of smoking and marination on the sensory characteristics of cold-cut chicken breast meat.

Influence of natural organic matter (NOM) coatings on nanoparticle adsorption onto supported lipid bilayers

Bo, Zhang,Avsar, Saziye Yorulmaz,Corliss, Michael K.,Chung, Minsub,Cho, Nam-Joon Elsevier 2017 Journal of hazardous materials Vol.339 No.-

<P><B>Abstract</B></P> <P>As the worldwide usage of nanoparticles in commercial products continues to increase, there is growing concern about the environmental risks that nanoparticles pose to biological systems, including potential damage to cellular membranes. A detailed understanding of how different types of nanoparticles behave in environmentally relevant conditions is imperative for predicting and mitigating potential membrane-associated toxicities. Herein, we investigated the adsorption of two popular nanoparticles (silver and buckminsterfullerene) onto biomimetic supported lipid bilayers of varying membrane charge (positive and negative). The quartz crystal microbalance-dissipation (QCM-D) measurement technique was employed to track the adsorption kinetics. Particular attention was focused on understanding how natural organic matter (NOM) coatings affect nanoparticle-bilayer interactions. Both types of nanoparticles preferentially adsorbed onto the positively charged bilayers, although NOM coatings on the nanoparticle and lipid bilayer surfaces could either inhibit or promote adsorption in certain electrolyte conditions. While past findings showed that NOM coatings inhibit membrane adhesion, our findings demonstrate that the effects of NOM coatings are more nuanced depending on the type of nanoparticle and electrolyte condition. Taken together, the results demonstrate that NOM coatings can modulate the lipid membrane interactions of various nanoparticles, suggesting a possible way to improve the environmental safety of nanoparticles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Interaction of Ag and C<SUB>60</SUB> nanoparticles with charged lipid membranes was studied. </LI> <LI> Quartz crystal microbalance experiments measured the adsorption kinetics. </LI> <LI> Natural organic matter (NOM) either inhibited or promoted nanoparticle adsorption. </LI> <LI> Adsorption profile depended on nanoparticle type, electrolyte condition, and NOM. </LI> </UL> </P>

Expression profile of an operationally-defined neural stem cell clone

Parker, Mark A.,Anderson, Julia K.,Corliss, Deborah A.,Abraria, Victoria E.,Sidman, Richard L.,Park, Kook In,Teng, Yang D.,Cotanche, Douglas A.,Snyder, Evan Y. Elsevier 2005 Experimental neurology Vol.194 No.2

<P><B>Abstract</B></P><P>Neural stem cells (NSCs) are the most primordial and least committed cells of the nervous system, the cells that exist <I>before</I> regional specification develops. Because immunocytochemically-detectable markers that are sufficiently specific and sensitive to define an NSC have not yet been fully defined, we have taken the strong view that, to be termed a “stem cell” in the nervous system—in contrast to a “progenitor” or “precursor” (whose lineage commitment is further restricted)—a <I>single neuroectodermally-derived cell</I> must fulfill an operational definition that is essentially similar to that used in hematopoiesis. In other words, it must possess the following functional properties: (1) “Multipotency”, i.e., the ability to yield mature cells in all three fundamental neural lineages throughout the nervous system—neurons (of all subtypes), astrocytes (of all types), oligodendrocytes—in multiple regional and developmental contexts and in a region and developmental stage-appropriate manner. (2) The ability to populate a developing region and/or repopulate an ablated or degenerated region of the nervous system with appropriate cell types. (3) The ability to be serially transplanted. (4) “Self-renewal”, i.e., the ability to produce daughter cells (including new NSCs) with identical properties and potential. Having identified a murine neural cell clone that fulfills this strict operational definition—in contrast to other studies that used less rigorous or non-operational criteria for defining an NSC (e.g., the “neurosphere” assay)—we then examined, by comparing gene expression profiles, the relationship such a cell might have to (a) a <I>multipotent</I> somatic stem cell from another organ system (the hematopoietic stem cell [HSC]); (b) a <I>pluripotent</I> stem cell derived from the inner cell mass and hence without organ assignment (an embryonic stem cell); (c) neural cells isolated and maintained primarily as neurospheres but without having been subjected to the abovementioned operational screen (“CNS-derived neurospheres”). ESCs, HSCs, and operationally-defined NSCs—all of which have been identified not only by markers but by functional assays in their respective systems and whose state of differentiation could be synchronized—shared a large number of genes. Although, as expected, the most stem-like genes were expressed by ESCs, NSCs and HSCs shared a number of genes. CNS-derived neurospheres, on the other hand, expressed fewer “stem-like” genes held in common by the other operationally-defined stem cell populations. Rather they displayed a profile more consistent with differentiated neural cells. (Genes of neural identity were shared with the NSC clone.) Interestingly, when the operationally-defined NSC clone was cultured as a neurosphere (rather than in monolayer), its expression pattern shifted from a “stem-like” pattern towards a more “differentiated” one, suggesting that the neurosphere, without functional validation, may be a poor model for predicting stem cell attributes because it consists of heterogeneous populations of cells, only a small proportion of which are truly “stem-like”. Furthermore, when operational definitions are employed, a common set of stem-like genes does emerge across both embryonic and somatic stem cells of various organ systems, including the nervous system.</P>

Chemical processing strategies to obtain sporopollenin exine capsules from multi-compartmental pine pollen

Arun Kumar Prabhakar,Hui Ying Lai,Michael G. Potroz,Michael K. Corliss,박재현,Raghavendra C. Mundargi,조대호,방사익,조남준 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.53 No.-

Pine pollen is widely used in traditional Chinese medicine and has been consumed as a food product for thousands of years. Owing to wind pollination, its pollen grains are composed of a sporoplasmic central cavity along with two empty air sac compartments. While this architectural configuration is evolutionarily optimized for wind dispersal, such features also lend excellent potential for encapsulating materials, especially in the context of preparing sporopollenin exine capsules (SECs). Herein, we systematically evaluated one-pot acid processing methods in order to generate pine pollen SECs that support compound loading. Morphological properties of the SECs were analysed by scanning electron microscopy (SEM) and dynamic imaging particle analysis (DIPA), and protein removal was evaluated by CHN elemental analysis and confocal laser scanning microscopy (CLSM). It was identified that 5-h acidolysis with 85% w/v phosphoric acid at 70 C yielded an optimal balance of high protein removal and preservation of microcapsule architecture, while other processing methods were also feasible with an additional enzymatic step. Importantly, the loading efficiency of the pine pollen SECs was three-times greater than that of natural pine pollen, highlighting their potential for microencapsulation. Taken together, our findings outline a successful strategy to prepare intact pine pollen SECs and demonstrate for the first time that SECs can be prepared from multi-compartmental pollen capsules, opening the door to streamlined processing approaches to utilize pine pollen microcapsules in industrial applications.

Effects of smoking and marination on the sensory characteristics of cold-cut chicken breast filets: A pilot study

Samant, Shilpa S.,Crandall, Philip G.,O'Bryan, Corliss A.,Lingbeck, Jody M.,Martin, Elizabeth M.,Tokar, Tonya,Seo, Han-Seok 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6

This study aimed to determine individual and combined effects of smoking and marination on the sensory characteristics of boneless, skinless chicken breast meat. Four types of cooked, cold-cut chicken breast meat, i.e., marinated cooked, marinated smoked, and controls of non-marinated cooked and non-marinated smoked chicken, were evaluated for 28 sensory characteristics. Marination significantly increased saltiness, sweetness, roasted flavor, smoked flavor, and moistness of the cold-cut chicken breast meat. In addition, smoking significantly enhanced the saltiness, bitterness, roasted flavor, smoked flavor, and moistness of mass. Interestingly, a combination of smoking and marination processes resulted in a synergistic increase in the perceived moistness of mass compared to their individual treatments. In conclusion, this study demonstrates individual and combined influences of smoking and marination on the sensory characteristics of cold-cut chicken breast meat.

Chemical processing strategies to obtain sporopollenin exine capsules from multi-compartmental pine pollen

Prabhakar, A.K.,Lai, H.Y.,Potroz, M.G.,Corliss, M.K.,Park, J.H.,Mundargi, R.C.,Cho, D.,Bang, S.I.,Cho, N.J. Korean Society of Industrial and Engineering Chemi 2017 Journal of industrial and engineering chemistry Vol.53 No.-

Pine pollen is widely used in traditional Chinese medicine and has been consumed as a food product for thousands of years. Owing to wind pollination, its pollen grains are composed of a sporoplasmic central cavity along with two empty air sac compartments. While this architectural configuration is evolutionarily optimized for wind dispersal, such features also lend excellent potential for encapsulating materials, especially in the context of preparing sporopollenin exine capsules (SECs). Herein, we systematically evaluated one-pot acid processing methods in order to generate pine pollen SECs that support compound loading. Morphological properties of the SECs were analysed by scanning electron microscopy (SEM) and dynamic imaging particle analysis (DIPA), and protein removal was evaluated by CHN elemental analysis and confocal laser scanning microscopy (CLSM). It was identified that 5-h acidolysis with 85% w/v phosphoric acid at 70<SUP>o</SUP>C yielded an optimal balance of high protein removal and preservation of microcapsule architecture, while other processing methods were also feasible with an additional enzymatic step. Importantly, the loading efficiency of the pine pollen SECs was three-times greater than that of natural pine pollen, highlighting their potential for microencapsulation. Taken together, our findings outline a successful strategy to prepare intact pine pollen SECs and demonstrate for the first time that SECs can be prepared from multi-compartmental pollen capsules, opening the door to streamlined processing approaches to utilize pine pollen microcapsules in industrial applications.