Postnatal development of T-lymphocyte subpopulations in the intestinal intraepithelium and lamina propria in chickens

Lillehoj, Hyun, S.,Chung, Kyeong S. 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

Lillehoj, H.S. and Chung, K.S., l992. Postnatal developmemt of T-lymphocyte subpopulations in the intesinal intraepithelium and lamina propria ln chickens. Vet. Immunol. Immunopathol., 31:347-360. Postnatal development of various T-lymphocyte subpopulations expressing CD3, CD8, CD4, and antigen-specific TCR heterodimers αβ(TCR2) or γδ(TCRl) was investigated in two different inbred chicken strains, SC and TK. The ratios of jejunum T-cells expressing TCRl to TCR2 in the intraepithelium of SC and TK strains gradually increased after hatching and were 3.40 and 4.28 by l2 weeks in TK and SC chickens respectively. The ratios of TCRl^+ to TCR2^+-cells in intraepithelium and the lamina propria in SC chickens were 0.96 and l.23 at 8 weeks and 4.29 and 2.15 at 12 weeks, respectively. Jejunum intraepithelial lymphocytes expressing the CD8 antigen increased gradually until 4-6 weeks of age and subsequently declined as chickens aged. CD4^+-cells represented a minor subpopulalion among the intestinal lymphocyte subpopulations. Therefore, the composition of various T-cell subpopulations in the intestine depended upon host age, the regions of the gut examined and host genetic background. These results suggest that changes in T-cell subpopulations in the intestine may reflect age-related maturation of the gut-associated lymphoid tissues.

Dynamic assembly of Hda and the sliding clamp in the regulation of replication licensing

Kim, Jin S.,Nanfara, Michael T.,Chodavarapu, Sundari,Jin, Kyeong S.,Babu, Vignesh ,M. ,P.,Ghazy, Mohamed A.,Chung, Scisung,Kaguni, Jon M.,Sutton, Mark D.,Cho, Yunje Oxford University Press 2017 Nucleic acids research Vol.45 No.7

<P><B>Abstract</B></P><P>Regulatory inactivation of DnaA (RIDA) is one of the major regulatory mechanisms of prokaryotic replication licensing. In RIDA, the Hda–sliding clamp complex loaded onto DNA directly interacts with adenosine triphosphate (ATP)-bound DnaA and stimulates the hydrolysis of ATP to inactivate DnaA. A prediction is that the activity of Hda is tightly controlled to ensure that replication initiation occurs only once per cell cycle. Here, we determined the crystal structure of the Hda–β clamp complex. This complex contains two pairs of Hda dimers sandwiched between two β clamp rings to form an octamer that is stabilized by three discrete interfaces. Two separate surfaces of Hda make contact with the β clamp, which is essential for Hda function in RIDA. The third interface between Hda monomers occludes the active site arginine finger, blocking its access to DnaA. Taken together, our structural and mutational analyses of the Hda–β clamp complex indicate that the interaction of the β clamp with Hda controls the ability of Hda to interact with DnaA. In the octameric Hda–β clamp complex, the inability of Hda to interact with DnaA is a novel mechanism that may regulate Hda function.</P>

Nested PCR Detection of Chrysanthemum stunt viroid (CSVd) from Chrysanthemum Cultivar Seedlings

Jae-Kyoung Shim,S.M. Hemayet Jahan,Bong-Nam Chung,Sukchan Lee,Chang-Kil Kim,Kyeong-Yeoll Lee 한국화훼산업육성협회 2013 화훼연구 Vol.21 No.3

Chrysanthemum stunt viroid (CSVd) is a serious pathogen affecting chrysanthemum that has caused significant economic losses to Chrysanthemum flower production worldwide. Control of CSVd disease is difficult due to its contagious nature and long latent period in the field. As chrysanthemum is most often produced by implanting seedlings, it is necessary to diagnose CSVd infection before cultivation. In this study, we screened CSVd infection in seedlings from 30 varieties including 5 domestic, 6 Japanese, and 19 European varieties. Molecular diagnosis of the combination of RT-PCR and nested PCR showed that CSVd was not detected by the first RT-PCR but detected by the second nested PCR analysis in 10 varieties, including 1 domestic, 2 Japanese, and 7 European varieties. Further comparison of 10 identified CSVd nucleotide sequences showed that those are highly conserved (99-100%) and the most similar to an isolate (AB006737) identified in Hokkaido, Japan. Our study suggests that the combination of RT-PCR and nested PCR analysis is successful for the CSVd diagnosis of seedlings and the molecular diagnosis is necessary to prevent the introduction and propagation of viroid disease into the fields.

영지버섯 생장점 단백다당체 GLB의 대식세포 활성화 효과

오정연(Jung Yeon Oh),조경주(kyung Joo Cho),정수현(Soo Hyun Chung),김진향(Jin Hyang Kim),Hyun S. Lillehoj,정경수(Kyeong Soo Chung) 대한약학회 1998 약학회지 Vol.42 No.3

In the previous study we described the antitumor activity of GLB, a protein-polysaccharide fraction of the growing tips of Ganoderma lucidum, against sarcoma 180 solid tumor in ICR mice. In this study we investigated the stimulatory activity of GLB on macrophages. When analyzed using a flow cytometer, GLB (100mcg/ml) was found to increase the phagocytic activity of the BALB/c mouse peritoneal macrophages as well as chicken macrophage BM2CL cells against FITC-labeled C.albicans by 55.2% and 21.2%, respectively. GLB also increased the spreading and the expression of MHC class II molecules of BM2CL cells as well as the mouse peritoneal macrophages. From these results, it is clear that GLB is a strong stimulator to the macrophages.

Association analysis of formyl peptide receptor 2 (FPR2) polymorphisms and aspirin exacerbated respiratory diseases.

Kim, Hee-Jeong,Cho, Sung-Hwan,Park, Jong-Sook,Lee, Tae-Hyeong,Lee, Eun-Ju,Kim, Yong-Hoon,Uh, Soo-Taek,Chung, Il Yup,Kim, Mi-Kyeong,Choi, Inseon S,Park, Byung-Lae,Shin, Hyoung-Doo,Park, Choon-Sik Springer-Verlag 2012 Journal of human genetics Vol.57 No.4

<P>Aspirin-exacerbated respiratory diseases (AERD) are associated with the metabolism of arachidonic acid. FPR2 (formyl peptide receptor2) is a high-affinity ligand receptor for potent anti-inflammatory lipid metabolites: lipoxins. Thus, functional alterations of the FPR2 may contribute to AERD. We investigated the relationship between single-nucleotide polymorphisms (SNPs) in the FPR2 and AERD. Asthmatics were categorized into AERD <15% decreases in forced expiratory volume in one second (FEV(1)), and/or naso-ocular reactions after oral aspirin challenge (n=170) and aspirin-tolerant asthma (ATA, n=268). In all, 11 SNPs were genotyped. FPR2 protein expressions on CD14-positive monocytes in peripheral blood were measured using flow cytometric analysis. We performed RT-PCR of the FPR2 mRNA expressed by peripheral blood mononuclear cells. Logistic regression analysis showed that the minor allele frequency of FPR2 -4209T>G (rs1769490) in intron 2 was significantly lower in the AERD group (n=170) than in the ATA group (n=268) (P=0.006, P(corr)=0.04, recessive model). The decline of FEV(1) after aspirin challenge was significantly lower in the subjects with GG homozygotes of FPR2 -4209T>G than those with the other genotypes (P=0.0002). Asthmatic homozygotes for FPR2 -4209T>G minor allele exhibited significantly higher FPR2 protein expression in CD14-positive monocytes than did those with the common allele of FPR2 -4209T>G allele (P=0.01). There was no difference in the expression of the wild form and the exon 2 deleted variant form of FPR2 gene according to the genotypes of FPR2 -4209T>G. The minor allele at FPR2 -4209T>G may have a protective role against the development of AERD, via increase of FPR2 protein expression in inflammatory cells.</P>

영지버섯 생장점 단백다당체 GLB의 대식세포 활성화 효과

오정연,조경주,정수현,김진향,Lillehoj, H.S.,정경수 충남대학교 약학대학 의약품개발연구소 1998 藥學論文集 Vol.14 No.-

In the previous study we described the antitumor activity of GLB. a protein-polysaccharide fraction of the growing tips of Ganoderma lucidum, against sarcoma 180 solid tumor in ICR mice. In this study we investigated the stimulatory activity of GLB on macrophages. When analyzed using a flow cytometer. GLB (100 ㎍/㎖) was found to increase the phagocytic activity of the BALB/c mouse peritoneal macrophages as well as chicken macrophage BM2CL cells against FTTC-labeled C. albicans by 55.2% and 21.2%, respectively. GLB also increased the spreading and the expression of MHC class Ⅱ molecules of BM2CL cells as well as the mouse peritoneal macrophages. From these results, it is clear that GLB is a strong stimulator to the macrophages.