The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

강진만 해역의 다모류 군집의 종조성 및 계절변동

윤성민,김춘철,서원일,황선영,이성훈,한경호,이우범 國立麗水大學校 環境問題硏究所 2004 環境硏究論文集 Vol.6 No.-

To detemine seasonal fluctuations in abundance and speices composition, polychaete samples were collected by Van Veen grab form March to November 2003 in Kangjin Bay. A total of 25,119.3 ind./㎡ polycaete were sampled and identified into 48 species. 25 families and 10 orders. Of the 10 orders. Phllodocida, Spionida, Eunicida and Sabellida accounted for approximately 79.9% of the polychaete fauna in this area. Phyllodocida, Nereidae, Nephtyidae ans Spionidae were dominant representing every moment 4 species. Of 48 species identified Neanthes japonica, Prionospio pinnate, Cirratulus cirratus and Palola siciliensis for 54.0% individuals collected. Season succession of dominant species was evident in study area: Neanthes japonica, Prionospio pinnata. Cirratulus cirratus. Nephtys oligobranchia in March, Neanthes japonica, Lumbrineris japonica. Cirratulus cirratus, Prionospio pinnata in May, Ncanthes japonica, Cirratulus cirratus, Lumbrineris nippnica. Lumbrineris japonica in August and Neanthes japonica, Prionospio pinnata, Laonice cirrata, Palola siciliensis in November, respectively. The diversity index was the highest value in March(H'=3.0059) and the lowest value in May(H'=2.7305). The eveness index was the highest in August(J=0.9184) and lowest in May(J=0.8524). The dominance index was the highest in May(D=0.3843) and the lowest in March(D=0.2275).

지반진동 전파에 관한 연구

전종균,박윤창 선문대학교 첨단과학기술연구소 1998 첨단과학기술연구소 논문집 Vol.3 No.-

차량 또는 열차에 의해 발생되는 지반진동문제는 인근구조물의 피해와 주변생활환경을 악화시키는 요인으로 인식되고 있다. 이러한 지반진동문제는 주로 표명을 따라 전달되는 Rayleigh파의 영향이 가장 큰 것으로 알려져 있다. 본 연구는 Rayleigh 파의 지반전달특성을 검토하고 수치모형에 있어서의 매개변수를 산정하여 진동차단 방진구의 최적화설계에 필요한 기초자료(파의 파장 및 감쇠특성)를 수집하는데 그 목적이 있다. The ground vibration by vehicles or trains has been recognized one of the major factors to damage a near-by structures and environments. It has been known that this problem is the most affected by Rayleigh wave which carries the most of energies following the ground surface. By inspecting characteristics of the Rayleigh wave and parameters of numerical model, the aim of this study is to establish preliminary data(wave lengths and damping characteristics of wave) to optimize designing antivibration barriers.

관광객의 웹탐색 활동에서 플로우의 결정요인과 효과

천영수,김윤숙 제주대학교 관광산업연구소 2001 産經論集 Vol.15 No.2

자외선 조사에 의한 표피내 색소세포의 형태학적 변화에 관한 연구

全秀一,朴允基,李成洛 연세대학교 의과대학 1984 연세의대논문집 Vol.17 No.1

수용액에서 N-[(4,6-Disubstituted Pyrimidin-2-yl)Aminocarbonyl] Saccharin 유도체의 합성

윤무홍,이천수 啓明大學校 産業技術硏究所 1997 産業技術硏究所 論文報告集 Vol.20 No.-

N-[(4,6-Disubstituted pyrimidin-2-yl)aminocarbonyl]saccharin derivatives can be easily synthesized by treating the basic solution of alkyl or phenyl (2-methoxycarbonyl phenyl sulfonyl)carbamate is dissolved in excess potassium hydroxide aqueous solution, with the acidic solution of 2-amino-4,6-disubstituted pyrimidine ammonium chlorides. Their corresponding chemical structures are identified by IR, ¹H-NMR and ¹³C-NMR as follows: N-[(pyrimidin-2-yl)aminocarbonyl]saccharin(5), N-[(4,6-dimethylpyrimidin-2-yl)aminocarbonyl]saccharin(6),N-[(4-methoxy-6-methylpyrimidin-2-yl)amino-carbony]saccharin(7), N-[(4,6-dimethoxypyrimidin-2-yl) aminocarbonyl]saccharin(8), N-[(4-ethoxy-6-methylpyrimidin-2-yl]aminocarbonyl]saccharin(9), N-[(4-i-propoxy-6-methylpyrimidin-2-yl)aminocarbonyl]saccharin(10), N-[(4-butoxy-6-methylpyrimidin-2-yl)aminocarbonyl]saccharin(11).

충남대학교 기초과학분야의 효율적 육성에 관한 연구

윤화중,진성일,윤민중,윤혜수,이영하,최철규,이천배,신대현 충남대학교 자연과학연구소 1986 忠南科學硏究誌 Vol.13 No.2

Chungnam National University (CNU) is located in Daeduk Science Town, a center of science and technology in Korea, and it is expected to play an important role in the development of science in Korea. With this in mind, the basic natural sciences at CNU should be more actively supported in both education and research. Accordingly analysis and comparison of the basic science program at major college in Korea as well as those in the USA and Europe has been performed. The current cooperative relationship between CNU and research institutes in the science town has also been analyzed. This study has resulted in propositions to make more efficient the upbringing of the basic sciences at CNU, by focusing on the following points ; a) to improve academic affairs, b) to make graduate studies substantial, c) to secure highly qualified faculty members, d) to increase cooperation in research between CNU and reserch institutes, e) to enlarge research laboratories and acquire more equipment and facilities.

초등학교 체육수업에서의 문제행동 아동에 관한 연구

전윤선,김경숙 이화여자대학교 동작과학연구소 2004 이화체육논집 Vol.8 No.-

The objectives of this research are to analyze various types of behavioral probllems and their underlying causes within an elementary physical education class; to observe the different methods teachers aply in dealing with these behavior problems. The research isbased on the case studies of four students in the third and fourth grade. Their behaviours were observed in six classes. Additional data was collected through in-depth interviews with the children and their teachers. Interviews with children were conductedrandomly while the teachers were interviewed over two sessions. The data was analyzed under the constant comparative method and the reliability of the data was ennanced through subject checks, peer debriefing, and the triangulation of the data. The results of the research are as follows: First, through the analysis based on the observations of the children with behaviors were categonized into four types: ""over-action behaviors,"" ""aggressive behaviors,"" ""withdrawa-anxiety behaviors,"" and ""isolation behaviors."" Second, the results of the analysis on the causes of behavior problems indicated that the children were aware of their problems. The children's parents were also aware of their problems but were not able to come up with workable solutions to change their behavior. Third, among the four behavior types, teachers selected over-action behavior types and aggressive behavior types as the most disruptive in class and also the most problematic in social interactions with other students. The other two types, withdrawal-anxiety and isolation behavior types did not cause any problems. Consequently, It appears that it is necessary to consider a counterplan for teaching problem children effectively in many directions. Putting these various research results together, school is the place where children spent a lot of time. Especially s children have more physical activity and interaction with other children in physical class than any other classes, physical education class is the place where problem behaviors often happen. Since such problem behaviors hinder intellectural, emotional and physical development of children seriously and exert a bad influence on the class as a whole, teachers must instruct problem behavior children with suitable methods and study how to cope with the problem behavior through knowledge of problem behavior.

요통 환자에서 경막 외 스테로이드 주사의 효과 비교

박윤길,전중선,전세일 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.3

용수 재이용을 위한 소규모 오수처리시설의 UV 소독시설에 관한 연구

윤춘경,정광욱,함종화 建國大學校附設農業資源開發硏究所 2002 農資源開發論集 Vol.24 No.-

Deterministic and probabilistic approaches to the design of ultraviolet disinfection system for water reclamation are reviewed and discussed. The high inactivation of total coliform (TC), fecal coliform (FC) and Escherichia coli (E. coli) by UV disinfection was demonstrated, and the inactivations of TC, FC and E. coli were 97%, 98% and 99%, respectively. Within the range of 0.3∼0.4m Ws/cm, the effect of UV does on the inactivation ratio was not observed. However, in the highest wattage of UV lamp, 39W, the inactivation ratio of TC, FC and E. coli was 100%, regardless of the UV does so the UV density was more effective on inactivation ratio of TC, FC and E. coli rather than UV does. Under the 0.4 mWs/cm and 16W of UV lamp, the effect of dissolved organic matter and turbidity on the inactivations of TC, FC and E. coli could not be observed in this study within the range of 0∼32mg/L and 0-40 NTU, respectively. The effect of suspended solid is Tc, FC and E.coli observed liner equation y=305.03x-3697, y=186.14x-2509.5, y=152.96x-1976.5(r^=0.51, 0.69, 0.80) respectively. Suspended solid is the most important factor for design of the UV disinfection system.