http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Chun Kwon-Wook (검색결과 225 건)
- 무료
- 기관 내 무료
- 유료
-
권선옥,김우식,오명기,나종천,이홍기,조욱현,최석구 인제대학교 2006 仁濟醫學 Vol.27 No.-
The use of critical pathways for a variety of clinical conditions has grown rapidly in recent years, particularly pathways for patients with acute myocardial infarction. We intend to determine the impact of a clinical pathway on ST-elevation myocardial infarction (STEMI) and to evaluate the efficacy and safety of facilitated percutaneous coronary intervention (PCI) compared with primary PCI. Low risk STEMI patients (ST elevation >0.1mV in more than 2 limb leads or ST elevation >0.2mV in contiguous precordial lead, chest pain lasting more than 30 min without response to nitroglycerin) will be included. All patients will be also treated medically according to critical pathway. STEMI is one of the common diseases in emergency medicine and so it is necessary to establish realistic treatment guidelines. The use of critical pathways will improve the quality of care.
-
-
-
-
Anticancer and antiviral effects of an oxidative fumigant
Sunil Kumar,Hyunji Eo,Wonsoo Chun,Hyuk Kwon,Jahyun Na,Yongshik Chun,Wook Kim,Yonggyun Kim 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04
An oxidative fumigant is potent to kill insect pests infesting stored grains. Its oxidative activity generates reactive oxygen species (ROS), which has been considered to be a main insecticidal factor. Furthermore, the oxidative fumigant has cytotoxic effect to insect cell lines, but the cytotoxicity is abrogated by antioxidant treatment. This study aimed to extend the usefulness of the oxidative fumigant in terms of medical purpose against cancer cells. Five cancer cell lines HCT 116 (human colorectal), Lovo (human colorectal), SW480 (human colorectal), MDA-MB-231 (human breast), and MCF-7 (human breast) were tested to determine their susceptibility to the oxidative fumigant with reference to two insect cell lines (Sf9 and Hi-Five). All cancer cell lines were highly susceptible to the oxidative fumigant, compared to the insect cell lines. Interestingly, basal ROS levels of the cancer cell lines were much higher than the insect cell lines. Furthermore, the oxidative fumigant significantly increased the ROS levels in the cancer cells. Treatment of vitamin E as an antioxidant mitigated the cytotoxicity of the oxidative fumigant. Thus, the high susceptibility of cancer cells to the oxidative fumigant may be induced by their high inducible ROS production. This study also investigated the antiviral activity of the oxidative fumigant against insect and plant viruses. The oxidative fumigant significantly inactivated a baculovirus (dsDNA virus) by inhibiting polyhedral production in Sf9 cells. It also inactivated tobacco mosaic virus (ssRNA virus) by suppressing phytopathogenicity. These results support a broad effect of the oxidative fumigant, which can be applied to agricultural and medical purposes.
-
C -11 및 F - 18 표지 콜린의 합성과 체내동태에 관한 연구
전권수,유국현,김상욱,임상무,홍성운,서용섭,양승대,안순혁,허민구 대한핵의학회 2001 핵의학 분자영상 Vol.35 No.3
Objectives: Recently, [methyl-(11)^C]-(β-Hydroxyethyl)trimethylammonium ([(11)^C]choline) has been discovered to be a very effective tracer in imaging various human tumors using positron emission tomography. Because of the short half-life of C-11, it is very difficult to use in a routine imaging procedure and needs a frequent synthesis of [(11)^]choline. This can be supplemented by the substitution of [(11)^Ccholine with [methyS-18]fluorocholine. Here, we would like to report cell uptake and biodistribution of [(11)^Ccholine and [(18)^F]fluorocholine as a basic study. Methods [(11)^C]Choline was prepared by the treatment of [(11)^C]CHzI with N,N-dimethylaminoethanol and [18F]fluorocholine was synthesized from reaction of CHzBr[18F]F with N,N-dimethylaminoethanol. The radiochemical purity was checked by high performance liquid chromatography (HPLC). The biodistribution of [(11)^C]choline and [(18)^F]fluorocholine was determined in balb/c mouse at 5 min, 20 min, 40 min and 80 min. The cell uptake wa measured using glioma (9L) and colon adeocarcinoma (SW620). Results: The radiochemical purity was more than 98% after purification. In the liver, uptake did not change over time the uptake was 20/ID/g for [C]choline and 13%ID/g for [(18)^F]fluorocholine. In the kidney, radioactivity decreased over tirne the uptake was 15%1D/g for [(11)^Ccholine and 20%ID/g for [(18)^F]fluorocholine, 80 min post-injection. The cell uptake of [(11)^Ccholine was 4.93% for glioma (9L) and 18.69F for colon adenocarcinoma (SW620). For [(18)^F]fluorocholine, 1.77% for glioma (9L) and 2.77% for colon adenocarcinoma (SW620). Conclusion: [(11)^CCholine and [(18)^F]fluorocholine showed a different cell uptake tendency, depending on cancer cell line. (Korean J Nucl Med 200135:185-191)
-
-
Kwon, Seulgi,An, Sang Mi,Yu, Go Eun,Hwang, Jung Hye,Park, Da Hye,Kang, Deok Gyeong,Kim, Tae Wan,Park, Hwa Chun,Ha, Jeongim,Kim, Chul Wook,Plaizier, J. Canadian Science Publishing 2018 Canadian journal of animal science Vol.98 No.4
<P> Litter size is an important trait in the pig industry. Therefore, a lot of effort has been put into improving this trait. DNA methylation is an essential epigenetic modification present in unique DNA sequences. Alterations in methylation can affect transcription and phenotypic variation. The purpose of this study was to investigate the effect of DNA methylation on litter size. Methylation-specific restriction enzymes are simple and useful tools for detecting DNA methylation status. We used a pair of methylation-sensitive isoschizomers, which have the same recognition site, HpaII and MspI. Insulin-like growth factor binding protein 4 (IGFBP4) is a key regulator of ovarian follicular development and fetal growth in eutherian mammals. In this study, we discovered that IGFBP4 was hyper-methylated in the uterus tissue of a larger litter size group using bisulfite sequencing, and validated the positive relationship between the methylation status of IGFBP4 and the total number born of pigs using the porcine methylation-specific restriction enzyme polymerase chain reaction (PMP) assay. We suggest that the IGFPB4 gene can be used as a prognostic biomarker for hyperprolific sows and that the PMP assay is a useful tool for methylation status screening. </P>
-
權旭鎭,徐城鐸,全燾基 慶北大學校 醫科大學 1976 慶北醫大誌 Vol.17 No.2
抗菌劑를 投與받은 患者群과 받지않은 醫師 또는 學生群의 大便中 大腸菌의 藥劑耐性을 檢査하였던바 患者群의 菌에는 chloramphenicol, tetracycline, streptomycin, sulfisomidine 및 ampicillin(AP)에 對한 耐性菌株는 全分離菌의 60%以上이였으나 醫師群과 學生群에는 30%以下였으며 醫師群과 學生群사이에는 別差異가 없었다. 이들 耐性菌은 4劑以上에 多藥劑耐性인 것이 大部分이었고 感受性菌은 醫師群 및 學生群에서는 約70%以上이었으나 患者群에서는 22.4%였으며 耐性菌 保有者도 患者群에 越等히 많았다. 耐性菌의 耐性傳達率은 藥劑에 따라서 多少의 差異는 있었으나 患者群과 他群사이에 別로 큰 差異가 없었고 大體로 多藥劑耐性균의 耐性傳達率이 높았다. 傳達되는 耐性의 pattern을 보면 그 菌의 耐性全部를 傳達하는 것이 많았으나 一部만을 傳達하는 것도 있었고 AP耐性이 特히 잘 傳達되는 것을 보았다. In order to know the distribution of drug-resistant and R factor-bearing Escherichia coli in humans, the organisms isolated from stools of inpatients who received various antimicrobial treatment, doctors, and students were subjected for the study. About 60% of strains isolated from patients were resistant to chloramphenicol, tetracycline, streptomycin, sulfisomidine and ampiciliin(AP), but the strains resistant to these drugs were less than 30% among E. coil isolated from doctors and students, without marked difference between doctors and students. Most of the resistant strains were multiply resistant to 4 or more drugs. The strains susceptible to all test drugs were 22.4% amnng patient isolates and appaoximately 70% among isolates from doctors and students. The persons carrying resistant strains were more frequently encountered among patients than doctors and students. About 60% of resistantt strains transferred their resistance to E. coli ML 1410. The rate of transfer differed by the drugs used but not by the origins of strains. Some strains transferred their complete resistance patterns to the recipients, and some others transferred no or partial resistance to the recipient. AP resistance was most frequently transferred than other drugs used.
-
Kwon, Byung-Su,Jung, Heung-Su,Song, Min-Sun,Cho, Kyung Sook,Kim, Sung-Chun,Kimm, Kuchan,Jeong, Jin Sook,Kim, In-Hoo,Lee, Seong-Wook Elsevier 2005 Molecular therapy Vol.12 No.5
<P><B>Abstract</B></P><P>In this study, we describe a novel approach to human cancer therapy that is based upon <I>trans</I>-splicing ribozyme-mediated replacement of cancer-specific RNAs with new transcripts that exert therapeutic activities. We have developed a specific ribozyme that can reprogram human telomerase reverse transcriptase (hTERT) RNA to induce transgene activity selectively in cancer cells that express the RNA. The ribozyme-mediated triggering of the transgene expression was accomplished via a high-fidelity <I>trans</I>-splicing reaction with the targeted residue in the hTERT-expressing cells. The ribozyme also induced cytotoxic activity in various hTERT-expressing cancer cells, hence selectively retarding the growth of those cells. Efficient and specific cell regression was also detected with ganciclovir (GCV) treatment only in hTERT-positive cancer cells, which were established to express stably the specific ribozyme that contains the herpes simplex virus thymidine kinase (HSV-<I>tk</I>) gene. Tissue-specific expression of the ribozyme could further augment the target specificity of the ribozyme. Importantly, we observed efficient regression of tumors with GCV treatment in mice that had been inoculated subcutaneously with hTERT-positive cancer cells that stably expressed the specific ribozyme that contains HSV-<I>tk</I>. These results suggest that the hTERT RNA-targeting <I>trans</I>-splicing ribozyme could be a powerful agent for tumor-targeted specific gene therapy.</P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
