Study of decreased melanin production through p53 by heme oxygenase-1 inhibitor in normal human melanocytes

( Hee Sun Lim ),( Sun A Jin ),( Jee Bum Lee ),( Seong Jin Kim ),( Seung Chul Lee ),( Young Ho Won ),( Sook Jung Yoon ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Heme oxygenase (HO)-1 is induced by oxidative stress and plays important roles in anti-apoptosis and the rapid growth of several solid tumors. p53 has a central role in skin pigmentation and may impact onmelanoma at all stages. However, there has been little study of HO-1 in relation with p53 on melanin production Objectives: To know the effect of HO-1 on melanogenesis through p53 in normal human melanocytes Methods: Human melanocytes (hM) were primarily cultured from foreskin. After incubation, cells were rinsed twice with PBS then transfection with p53 siRNA Results: Melanin content was detected with ELISA and Western blot analysis and RT-PCR of tyrosinase, MITF were performed after ZnPP treatment and p53 transfection. After ZnPP treatment, melanin content was decreased, and tyrosinase and MITF protein levels were decreased. Tyrosinase and MITF mRNA levels were also decreased. However, melanin content and tyrosinase and MITF protein levels were increased after CoPP treatment. After p53 transfection, HO-1 expression was decreased when HO-1 stimulator was treated. In addition, melanin content was decreased, and tyrosinase expression was decreased in Western blot analysis Conclusion: These results suggest that melanogenesis is inhibited by ZnPP by decreased melanin production, tyrosinase and MITF protein and mRNA levels. Furthermore, those inhibitory effects of ZnPP may be dependent on p53 in normal human melanocytes. Therefore, HO-1 may play an important role in melanogensis

Upregulation of heme oxygenase-1 by ginsenoside Ro attenuates lipopolysaccharide-induced inflammation in macrophage cells

Sokho Kim,Myung-Hoon Oh,Bum-Seok Kim,Won-Il Kim,Ho-Seong Cho,Byoung-Yong Park,Chul Park,Gee-Wook Shin,Jungkee Kwon 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.4

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been suffi- ciently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to 200mM) for 1 h before treatment with 1 mg/ mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

Upregulation of heme oxygenase-1 by ginsenoside Ro attenuates lipopolysaccharide-induced inflammation in macrophage cells

Kim, Sokho,Oh, Myung-Hoon,Kim, Bum-Seok,Kim, Won-Il,Cho, Ho-Seong,Park, Byoung-Yong,Park, Chul,Shin, Gee-Wook,Kwon, Jungkee The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.4

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been sufficiently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to $200{\mu}M$) for 1 h before treatment with 1 mg/mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

망막전위도의 전력 산출

원철호,조진호,김상하,임정훈 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

주파수영역에서 망막전위도의 총전력을 계산하고, 시간영역에서 망막전위도의 면적을계산할수 있는 프로그램을 개발함으로써 전기생리학적 진단의 효능을 향상시키는데 목적을 두었다. 총전력은 망막전위도 신호를 Fourier 변환을 이용하여 주파수 영역의 신호로 바꾼 다음 스펙트럼의 제곱을 적분함으로써 구하였다. 우세전력은 우세주파수의 전력으로 이부분의 전압을 제곱후 적분하여 얻은 값이다. 원하는 부분은 수동입력하여 구간주파수의 제곱적분을 할 수 있게 하였다. 이 소프트웨어는 시간 영역에서 진동양소파의 진폭의 합 및 면적도 구할 수 있는 기능을 가졌으며, 사용자에게 편리하도록 그래픽 환경 하에서 마우스와 팝업 메뉴에 의하여 각 기능들이 작동되며, UTAS-E2000 시스템과도 상호 호환성을 갖고 있다(한안지 38:817∼823, 1997). The Calculation of the Electroretinographic Power Chul Ho Won*, Jin Ho Cho, Ph.D.**, Sang Ha Kim, M. D., Jung Hoon Lim, M. D. To promote the electrophysiologic diagnostic efficiency, the program for calculation of the total power in the frequency domain and of the area in the time domain of the electroretinogram was designed. The total power is calculated by integration of the squared power spectrum obtained by Fourier transform (Parseval theorem) in the time domain. The dominant power is the power of the dominant frequency in power spectrum and is calculated by integration of the squared peak area spectrum. The sectional integration of the square power spectrum can be established by manual input. The implemented software also has the functions that calculate the summed amplitude and area of the oscillatory potentials in the time domain. And it performs each functions by selection of pop-up menu with mouse to use conveniently under graphic interface, compatible with UTAS-E2000 system (J Korean Ophthalmol Soc 38:817∼823, 1997).

UASB 공정에 의한 고농도 축산폐수 처리시 유기물 제거와 메탄생성에 관한 연구

원철희,김승호,박은영,임재명 江原大學校 産業技術硏究所 2002 産業技術硏究 Vol.22 No.B

This research was performed to investigate the COD removal efficiency and methane production in slurry-typed swine wastes using UASB(upflow anaerobic sludge blanket)reactor. The USAB reactor was operated from 0.8 through 3.3days of HRT in a range of 3 to 15 kg TCOD/m³/day of volumetric organic loading rate. The removal rate of TCOD was increased with the increase of the HRT. The removal rate of TCOD at an HRT over 2days, became greater than 68% with the methane contents being from 70 to 80%. Methane production rates were increased from 0.27 to 0.36 m³CH₄/kg CODrem. as HRTs were increased from 0.8 to 3.3days.

X선 CT 영상으로부터 인체의 기관지 모델의 개발

원철호,노철균 한국센서학회 2002 센서학회지 Vol.11 No.5

본 논문에서는 X선 컴퓨터 단층 촬영으로부터 획득된 기관지의 3차원 영상으로부터 기관지의 구조 및 형태 정보를 추출할 수 있는 가능성을 조사하고, 분진 침착 모델의 수학적인 개발과 실험적인 검증에 사용될 수 있는 물리적인 공동 주형 모델을 개발한다. CT로부터 획득된 영상으로부터 수학적 모폴로지 기법에 의하여 폐의 기관지 영역을 검출한다. 검출된 폐의 기관지 영역의 체적 데이터를 3차원 공간상에서 나타낼 수 있는 표면 데이터를 STL(streolithography) 파일로 변환한 후에, 고속 프로토타입 기기에 입력하여 3차원 입체 형상을 제작한다. 이때 기존의 기관지 팬텀과 실제 정상인으로부터 획득된 CT 영상으로부터 각각의 공동 주형 모델을 제작하게 된다. CT 스캔하여 얻어진 원래의 영상과 제작된 공동 주형 모델을 CT 스캔하여 얻어진 영상에서의 기관지 영역의 단면 직경을 비교하여 생성된 폐 기관지 모델의 유용성을 검증하였다. In this paper, we investigate the potential for retrieval of morphometric data from three dimensional images of conducting bronchus obtained by X-ray Computerized Tomography (CT) and to explore the potential for the use of rapid prototype machine to produce physical hollow bronchus casts for mathematical modeling and experimental verification of particle deposition models. We segment the bronchus of lung by mathematical morphology method from obtained images by CT. The surface data representing volumetric bronchus data in three dimensions are converted to STL(streolithography) file and three dimensional solid model is created by using input STL file and rapid prototype machine. Two physical hollow cast models are created from the CT images of bronchial tree phantom and living human bronchus. We evaluate the usefulness of the rapid prototype model of bronchial tree by comparing diameters of the cross sectional area bronchus segments of the original CT images and the rapid prototyping-derived models imaged by X-ray CT.

西部慶南地域의 느타리버섯 裁培 및 疾病 發生 現況

김철호,조용운,김홍출,이상원 진주산업대학교 1999 論文集 Vol.38 No.-

The authors surveyed the present situation of Agaricus sp cultivation and its disease occurence in Western Kyong-Nam area. 192 farmers, which is 66% of the mushroom-cultivating farmers, were growing Agaricus sp. Especially there were 124 farms in Western Kyong-Nam around Chinju-City. The greater part of mushroom-farmers cultivated Agaricus sp. on tables using cotton as major medium. And they purchased the eldest mycelium from other districts. About 60% of the farmers were suffering some losses due to the development of disease. The major disease seemed to be brown blotch. When a mushroom-disease has developed, most farmer coped with it by removing the cotton media, and sometimes by treating with chemicals. Mushrooms seemed to be a major source of farmers' income, and some govenmental and scientific supports are needed for the development of mushroom cultivation.

기관지 탄분 섬유화증 환자의 임상 경과와 고감도 C-반응 단백(high sensitivity C-reactive protein, hsCRP)과의 상관 관계 비교

이규원,옥철호,장태원,정만홍 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Background: To investigate the association between bronchial anthracofibrosis(AF) and tuberculosis(TB). and the clinical utility of high sensitivity C-reactive protein(hsCRP) for diagnosis of active pulmonary TB in patients with bronchial AF. Method: Eighty three patients(62 women and 21 men ranging in age from 55 to 87), who were diagnosed with bronchial AF by a bronchoscopy, were enrolled in this study. The hsCRP was measured in patient's blood. The hsCRP results were compared with the bacteriological, and clinical finding. Results: The common respiratory symptoms were cough(70%), sputum(35%), dyspnea(20%), and hemoptysis(10%). The sputum AFB smear, culture were positive in 20(22.8%) and 37(44.5%) patients, respectively. The level of hsCRP in patients with postive culture was significantly higher than that of patients with negative culture.(4.67±7.5, 0.90±1.59, p=0.005). Of 63 patients with negative smear, the level of hsCRP in patients(n=18) with positive culture was higher than that of patients(n=45) with negative culture.(3.39±5.78, 0.92±1.50, p=0.013). Of 20 patients with negative smear and positive culture who were empirically treated with anti-TB medication, 11 patients showed radiologic response. and their level of hsCRP was higher than that of 9 patients who showed no radiologic response.(1.56±1.89, 0.81±0.10,p=0.03) Conclusion: hsCRP appears to be useful diagnostic tool for predicting active pulmonary tuberculosis. in patients with bronchial AF.

벼 담수직파 재배시 생장조절제 처리가 생육 및 도복에 미치는 영향

金正鎬,吳成煥,李哲遠,宋凡憲,孫錫龍 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

본 시험은 벼 도복을 경감시키기 위한 재배대책을 강구하기 위하여 추청벼를 공시하고, 담수직파재배에서 생장조절제를 처리함에 따른 도복경감효과를 조사하였던 바 그 결과를 요약하면 다음과 같다. 1 간장은 무처리구보다 IBP+Inabenfide구가 186cm 단축되어 가장 큰 감소효과를 보였다 2 하위절간장 역시 무처리구보다 IBP+Inabenfide구가 3-4절간 35cm, 4-5절간은29cm 단축되어 가장 큰 감소효과를 보였다 3 포장도복은 무처리 5에 비해 IBP+Inabenfide구는 0으로 도복이 발생하지 않았으며, 수량은 무처리에 비해 도복이 발생하지 않았던 IBP+Inabenfide구가 5% 증수되었다 This study was conducted to investigate the effects of plant growth regulators, 2,4~D, IBP, and Inabenfide, on growth responses and lodging resistance of rice in cultivation of the waterlogging direct seeding The Chuocheong cultivar of Japonica type was used Growth responses itcluding culm leagth and lodging index etc and rice yields were measured at major growth stages and harvest time Culm length was the shortest in the treatment of IBP + Inabenfide, shortening it to 186 cm compared to that of the control, showing the best application effect of the plant growth regulator The length of internode between third and fourth internodes of lower culm was shortened to 3.5 cm with treatment of IBP + Inabenfide compared to that of the control and it between fourth and fifth internodes was shortened to 29 cm with treatment of Inabenfide, showing the best effectiveness. Lodging was not appeared in treatment of IBP + Inabenfide compared to 5.0 of the control Resulting to reduciag the lodging, rice yield was increased more with treatment of IBP + Inabenfide, to about 50%, than that of the control

디지털카메라를 이용한 3차원 측량 기법 개발에 관한 연구

崔哲淳,崔勝弼,韓元浩,金東熙 관동대학교 1997 關大論文集 Vol.25 No.2

Photographic survey is possible that it can survey the simultaneous three-dimensional survey about so many points, therefore it is so fast and precisely. We takes a snap the objects with digital carema, and input the data to the computer from this data. We can take the three-dimensional coordinate through relative orientation, absolute orientation and have to check the merit of three-dimensional suveying. Otherwise we can get the error in one centimeter after analysis three-dimension coordinate and we can get the higher precise accuracy cause to set the camera angle of 20∼45 degrees.