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이용석,유주순,정수열,박춘수,최용락 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1
본 연구는 폐수 중의 질소 제거를 위한 생물학적 처리용 미생물 개발을 위한 목적으로 질소의 산화 능력이 뛰어난 균주를 분리하였다. 분리된 세균 중에서 질소 산화능과 생육 속도가 뛰어난 CH-N 균주를 선별하였으며, 생리, 생화학적인 특성 조사에 의해 Bacillus sp로 추정되어 Bacillus sp. CH-N이라 명명하였다. 분리 균주는 0.5% glucose가 포함된 초기 pH가 7.0인 암모니아 및 아질산성 질소 함유 배지에서 30시간 배양 후 가가 85%와 90%의 암모니아성과 아질산성 질소가 단시간에 질소의 감소율을 나타내었다. 폐수 및 생활하수에 분리 균주를 이용한 결과, 수질 속의 암모니아성 질소가 단시간에 크게 감소시키는 효과를 확인하였다. 균주를 고정시킨 담체의 질소산화 효과를 시험하고자 Bacillus sp. CH-N을 고정시킨 세라믹 담체를 이용한 결과, 배양 2일 후에는 암모니아성 질소가 전부 제거되었다. In order to improve the system for biological nitrogen oxidizing process in sewage and wastewater, a bacterium having high abilities to oxidize of nitrogen was isolated from wastewater and polluted soils. The strain was identified to Bacillus sp. CH-N, based on the physiological and biochemical properties. Characteristics and oxidizing ability of both ammonia and nitrite were examined for the strain, Bacillus sp. CH-N. The strain showed the oxidizing rate about 80% to 90% on the sewage and wastewater after 48 h culture. The nitrogen oxidizing rate was increased in proportion to the initial concentration of glucose. The microorganism, Bacillus sp. CH-N cell immobilized on ceramic carrier were evaluated for the oxidation of ammonia in culture media.
한국어 대용량 음성인식에서의 효율적인 시간 감축에 관한 연구
장춘서,이기수,신윤식,임은기,김병만,김현수 금오공과대학교 산업기술개발연구원 2000 産業技術開發硏究 Vol.16 No.-
In this paper a time reduction algorithm for large vocabulary speech recognition system has been studied. TDNN(Time Delay Neural Network) was used to classify vowel using phonetical fetures. To increase performance of vowel classification, modular TDNN architecture associated with 2 states input layer has been suggested.
張椿瑞,李琦秀 金烏工科大學校 1988 論文集 Vol.9 No.-
In this paper, an effective hardware architecture to implement isolated word real-time speech recognizer has been studied. Filter bank analysis and VQ(Vector quantization) technique have been used to reduce speech processing time and DTW(dynamic time warping) has been used for time alignment. The hardware of speech recognizer consists of two parts : controller part ad DSP part. Controller part has a buffer control logic for common memory and SIO for interfacing to host computer. DSP part with 16bit 40 Mhz high speed processor(TMS 32025) performs recognition operation. In order to perform feature extraction and VQ encoding with in one frame, time requirement for data memory access and memory bank architecture have been suggested.
최재춘,이수원 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.2
This study was conducted to investigate and compare the appearance and nutritional composition of the soy protein formulas, so as to get fundamental data for improvement of quality and competitive-power imported soy protein base formula. The results were summarized as follows: 1. In March, 1993, 3 companies were selling 3 soy base formulas which were packed in tin can, the amounts is 350-400gr. 2. Comparing the appearance of soy base formulas, here are 2 types of powder and 1 granular type. It is necessary to change the weaning food into granular type in order to improve the quality of soy base formulas. 3. The nutrient composition of macro-nutrients, vitamins and minerals are indicated in correspond with infants ages and weights. And these are suitable for recommended dietary allowances for Korean infants. It is recommended to add L-carmtine taurine, copper, vitamin-K, choline and MCT-oil which is easy to deficient in soy base formula and fructooli gosaccaride or growth promoting ingredients of the lactic-acid bacteria. 4. It is necessary to fortify for soy base formula the flavor-improving ingredients (yeast extract, sea tangle etc) like the soy bean flavor. 5. In order to imporve soy protein base formulas, it is necessary to make the products at the base of the age, weight and nutritional requirement of infants. Also, it is needed to develop high quality products which are fortified new ingredient (minor ingredients), vitamins and minerals similar to human milk ingredient.
방선균 WCM-9가 생산하는 Polyene 항진균성 항생물질(AF1)의 생물학적 성상
신운섭,정선호,이동희,이경호,김수기,박주영,고춘명 대한의진균학회 1996 대한의진균학회지 Vol.1 No.1
Background: Pathogenic fungi infect humans, especially immunocompromised patients, with superficial or deeply invasive pattern. In the past 20 years, fungal infections have been increased dramatically resulted by increment of organ transplantation, cancer, AIDS patients, or use of broad-spectrum antibacterial agents. Fungal infections are now important causes of morbidity and mortality of hospitalized patients. but there is no effective antifungal antibiotics as well as antibacterial antibiotics Objective: Effective new antifungal antibiotics are needed for the treatment of mycosis. So in an effort to develop effective antifungal antibiotics, we screened over 600 isolates of Streptomyces spp. from soil. Methods: Antifungal producing strain was selected using disk diffusion method, An antifungal substance (AF1) was purified with ethyl acetate extraction, silica gel column chromatography and reverse phase HPLC. MICs of AF1 were detected by agar dilition method. Results: The compound showed UV maxima of 307, 321, 340, 359 nm indicating methylpentaene. Minimum inhibitory concentrations of the AF1 were 3.7 ㎍/ml against mold, and 3.7 - 7.4 ㎍/ml against Candida species. AF1 was also active against Cryptococcus neoformans, with MIC of 0.9 ㎍/ml. The concentration of AF1 for K^+ ion release from human red blood cell and hemolysis were 5 ㎍/ml. Conclusion: The antibiotic purified from culture broth of Streptomyces sp. WCM-9 was a polyene antifungal antibiotic which have broad spectrum antifungal activity.