회음부의 세포성 혈관섬유종

최혜정,김성남,김규래 대한병리학회 2001 Journal of Pathology and Translational Medicine Vol.35 No.3

흰쥐에서 심정지후 Pentoxifylline이 뇌의 허혈-재관류 손상에 미치는 효과

정시경,김영민,오동렬,최경호,박승현,이운정,박규남,유은영,이원재,김세경 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: Two major events occurring in the cerebral hemodynamics after successful resuscitation from cardiac arrest are reactive hyperemia and postischemic hypoperfusion. In this study, we examined the effect of Pentoxifylline(PTX) on the rat brain following cardiac arrest. Methods: Fourteen rats were anesthetized and artificially ventilated. Cardiac arrest was produced by chest compression and clamping of tracheal tube for 3 minutes in ketamine anesthetized rats. Circulation was restored by standard cardiopulmonary resuscitation methods. In 7 rats, PTX 10mg/kg was infused at 10min after cardiac arrest(PTX group). In the other 7 rats, same amount of normal saline was infused(control group). Results: In both groups, hemodynamic variables, neurologic deficit(ND) score and histopathologic findings of hippocampal CA1 neurons were observed. Hemodynamic variables and ND score were not significantly different between two groups. Delayed ischemic neurons of hippocampal CA1 were decreased in PTX group(2.2±2.4%) compared with control group(9.1±1.2%). Conclusions: We conclude that PTX prevented development of delayed ischemic neurons in hippocampal CA1 after cardiac arrest. PTX may be useful in emergency situations following cardiac arrest.

응급의료센터에서 신경색의 조기진단

최경호,오동렬,이원재,박규남,박승현,황두영,김형국,정시경,김영민,김세경 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

To evaluate appropriate diagnostic studies for renal infartion in emergency medical center, we analysed 33 patients of renal infarction treated in this hospital for last 8 years. Eleven patients, 4 trauma associated and 7 aortic disease associated patients were excluded. They are 11 males and 11 females with a mean age of 51 years. Fourteen came to the emergency medical center and seven came to the outpatient department, in the other one patient renal infarction was developed during hospitalization with other disease. On their past history they had hypertension in 9, valvular heart disease in 5, and diabetic mellitus in 4 patients. Abdominal or flank pain was noted in 17 of 22(77%). The other symptoms were vomiting, nausea, fever and so on. On microscopic examination of initial urine in hospital, 5 patients showed more than 10 red blood cells by high power field examination. The mean lactic dehydrogenase level was 1,239 I.U/L(normal range 218-472 I.U/L), while the mean aspartate aminotransferase and mean alanine aminotransferase were 51 I.U/L(normal range 13-36 I.U/L) and 44 I.U/L(normal range 5-33 I.U/L). Abdominal ultrasonography showed positive findings in 5 of 16(31%), of which 3 were confirmed by Doppler ultrasonography. In 18, computed tomography was done and all those showed positive findings of renal infarction(100%). In conclusion, it is important that identify the elevated lactic dehdrogenase level in case of any suspicion about renal infarction and confirm by computed tomography.

이옥소우라늄(VI)이온과 질소- 산소 주개원자를 가진 거대고리 리간드와의 착물 형성에 관한 연구

최규성,김정,김용복 慶南大學校 附設 基礎科學硏究所 1990 硏究論文集 Vol.2 No.-

수용액에서 디아자 크라운 에테르 계의 고리 원자 수가 15개에서 18개인 거대고리 리간드와 이옥소우라늄(VI) 이온 간의 착물 형성에 대한 안정도 상수를 전위차적정법으로 조사하였다. 착물의 안정도 상수는 NenOdienH₄ < NtnOdienH₄< Ntn(OH)OtnH₄< NtnOenH₄< NtnOtnH₄의 순이었으며, 이와같은 안정도 상수의 변화에는 리간드의 염기도와 주개 원자의 배열이 영향을 미치고 있었다. 한편, 모든 리간드는 이옥소우라늄(VI) 이온과 매우 안정한 착물을 형성하는 것으로 밝혀졌는데, NenOdienH₄와 NtnOdienH₄착물의 안정도 상수를 여러 온도에서 조사한 결과, 착물의 안정도에는 착물 형성 과정의 엔탈피 및 엔트로피 변화 모두가 기여하고 있었다. The complexation of 15 to membered diaza crown ethers with dioxouranium(Ⅵ) ion were studied by potentiometry in aqueous solutions. The order of stability constants were NenOdienH₄ < NtnOdienH₄< Ntn(OH)OtnH₄< NtnOenH₄< NtnOtnH₄. The basicity and the arrangement of donor atoms on the ligands were affected on the stabilities. And all ligands formed very stable complex with dioxouranium(Ⅵ) ion. The stability constants of NenOdienH₄and NtnOdienH₄complexes were measured at various temperatures. As the results, enthalpy and entropy change of the complexation were distributed on the complex stabilities.

선형 최적제어 관측기와 연관된 다항식 급수분석(Ⅰ)

최규정,車仁洙 조선대학교 기초과학연구소 1989 自然科學硏究 Vol.12 No.1

In this paper, a reduced-order compensatory observer can be effectively used to dicrease the gap between original states and observed states. The three polynomial expression (ie. Legendre, Laguerre, Talyor series) were used to approach the reduced-order compensatory observer states. The results of this study show that expansion coefficients truncated the first to the third term and that they are efficient enough to calculate errors in the original and observed states.

Dopamine Transporter Gene의 다형성과 알코올 의존증의 관련

최윤정,방승규,김정진,도규영,백인호 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.4

연구목적 : 도파민계는 알코올 추구와 같은 알코올 의존증과 관련된 행동에 중요한 역할을 한다. Dopamine transporter(DAT1)는 신경연접부위에서 도파민 재흡수에 관여하여 도파민 활성을 종결시키는 물질로 알려져 있다. 그러므로, DAT1 유전자가 알코올 의존증에 대한 감수성에 영향을 줄 수 있다. DAT1 유전자는 다형성을 보이는 반복된 40개의 염기서열(VNTR)을 갖는다. 본 연구에서는 DAT1 유전자 다형성과 알코올 의존증 및 알코올 의존증의 임상변인과의 관련성에 대하여 알아보았다. 방 법 : 알코올 의존증 환자 94명과 정상대조군 113명을 대상으로 말초혈액을 채취하여 분리된 DNA의 DAT1 유전자를 DNA 중합효소연쇄반응으로 증폭하였다. 증폭된 산물을 15% polyacrylamide gel에서 전기영동하여 나타나는 띠의 크기에 따라 유전자형을 구분하였다. 자료의 통계적 처리는 Fisher's exact test, oneway ANOVA를 사용하였다. 결 과 : 환자군과 정상대조군의 유전자형은 모두 Hardy-Weinberg equilibrium의 예상치와 유의한 차이가 없었다. 대립유전자는 7 repeats, 9 repeats, 10 repeats, 11 repeats의 4가지 종류로 구분되었고, 유전자형은 7/7 repeats, 7/10 repeats, 9/10 repeats, 10/10 repeats, 10/11 repeats의 5가지 형태를 보였다. 환자군과 대조군간에 DAT1 유전자형 및 대립유전자 빈도는 유의한 차이가 없었고, DAT1 유전자형에 따른 음주 시작 연령, 알코올 의존증의 발병 연령 및 잠복기간에서도 차이가 없었다. 알코올 의존증의 가족력이 있는 환자군과 없는 환자군은 DAT1 유전자형의 빈도에서 차이가 있는 경향을 보였고(χ²=6.912, p=0.081), DAT1 대립유전자의 빈도에서도 유의한 차이가 있었으며(χ²=10.193, p=0.008), lambda값은 0.046였다. 결 론 : DAT1 유전자의 다형성이 알코올 의존증의 유전적 소인이 될 가능성은 적은 것으로 보이나, 알코올 의존증의 가족내 유전과는 관련있을 것으로 시사된다. Objectives : Alcoholism is known to be a heritable disease. It has been hypothesized that dopaminergic systems play an important heritable role in human behavor related to alcohol dependence, such as alcohol seeking. Therefore, genes involved in this pathway, including dopamine transporter(DAT1), which is responsible for taking released dopamine back up into presynaptic terminals and terminating dopaminergic activity, are potential candidate that may affect susceptibility to alcoholism. Analysis of a 40-base pair(bp) repeat(VNTR) in the 3' untranslated region of the DAT1 gene revealed variable number of the repeat ranging from 3 to 11 copies. Therefore, in the present study, we examined the association between alcoholism and VNTR polymorphism of DAT1. Methods : Genomic DNA analysis with polymerase chain reaction(PCR) was used to identify the presence of a VNTR polymorphism. It was carried out within a group of 94 alcoholic patients and 113 normal controls. Results : 1) There were no significant differences in allelic or genotype frequencies between the group of alcoholic patients and controls. 2) There were no significant differences in the first drinking age, onset age and latency of alcoholism according to DAT1 genotypes. 3) There was a significant difference in allelic frequencies between alcoholics with family history and those without family history. Conclusions : These results suggested that VNTR polymorphism of DAT1 is unlikely to be a factor in the genetic etiology of alcoholism, but might be related to familial transmission of alcoholism.

정밀부품 및 기기에 대한 환경시험기술

최만용,박정학,윤규택 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.6

정밀부품이나 기기는 개발과정이나 완료단계에서 부적절한 설계에 의하여 발생할지도 모르는 잠재적인 결함을 방지하기 위하여 시험평가를 실시하게 된다. 본 논문에서는 신뢰성 기술 분야의 기초가 되는 환경시험기술을 소개 하였으며 엔코더, 교통신호제어기, 계측기기 및 위성항법 수신기 등 제품단위의 대상품에 대한 시험조건 및 방법 등을 기술하고 시험사례를 보고하였다. 이러한 사례를 통하여 제품의 신뢰성 향상을 위한 복합환경시험의 중요성을 강조하였고 최근 환경시험기술에 대한 인식이 날로 증대되면서 시험기술자 양성, 지그설계기술 그리고 고장해석 및 대책기술의 확보가 매우 필요함을 제안하였다. Precision parts and instruments are tested to evaluate performance in development-process and product-step to prement a potential defect due to a failure design. In this paper, Environmental test technology, which is the basis of reliability analysis, is introduced with examples of test criterion, test method for products, encoder and traffic signal controller, and measuring instruments. Recently, as the importance of the environmental test technology is recognized, It is proposed that tranining of test technician and technology of jig design and failure analysis are very essential.

Cu(Ⅱ) 이온과 질소-황을 가진 포단드와의 착물 형성에 관한 연구

최규성,정징운 慶南大學校 附設 基礎科學硏究所 1996 硏究論文集 Vol.8 No.-

The stability constants, enthalpy, and entropy changes of complexation of Cu(Ⅱ) ion with sulfur-containing podand ligands such as tri(phenylthio-2-ethyl)amine(Podand Ⅰ), tetra(phenylthio-2-ethyl)ethylendiamine (Podand Ⅱ) have been determined by uv-vis spectrophotometer in 95% MeOH at various temperature. From the results, the followings have been obtained. Cu(Ⅱ) ion forms a 1:1 complex with these ligands, the order of stability constants and enthaply changes is Podand Ⅰ <Podand Ⅱ, entropy changes is Podand Ⅰ> Podand Ⅱ.

자궁경부질도말에서 Atypical Glandular Cells of Undetermined Significance (AGUS)로 나타난 자궁경부의 표재성 자궁내막증 - 2예 보고 -

최혜정,이승구,김규래 대한병리학회 2001 Journal of Pathology and Translational Medicine Vol.35 No.3

햄스터 구강암 발생 과정에서 Heat Shock Protein에 관한 면역조직화학적 연구

최규환,이동근,김은철,정창주 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.2

A IMMUNOHISTOCHEMICAL STUDY ON HEAT SHOCK PROTEIN IN ORAL CARCINOGENESIS IN HAMSTER Heat shock protein (HSP) expression is unto·emulated in tumor cells and, HSP expression is likely marker of the malignant potential of oral epithelial lesion. Furthermore, the 70kDa HSP is implicated in the degree of tumor differentiation, the rate of tumor proliferation and the magnitude of the anti-tumor Immune response. Accordingly, the distribution and intensity of HSP70 and HSP47 expression was assessed in the DMBA induced oral carcinogenesis in hamster. Golden Syrian hamsters which were 3 months-age and 90∼120g were collected. 9,10-dimethyl -1,2-ben-zanthracene (DMBA) in a 0.5% solution in mineral oil was painted on the buccal pouch mucosa 3 times per week in the study group. In each control and experimental groups of 6, 8, 10, 12, 14, 16, 18, 20 weeks, specined for immunohistochemical study with anti-HSP47 and anti-HSP70 antibody. The following results were obtained. 1.HSP47 positive cells were race or negative of normal oral mucosa, increased mildly in basal and suprabasal basal layer, and spinous cell layer after experimental 6 weeks (dysplastic or CIS stage). In CIS stage, HSP47 expression is prominent in dysplastic free or normal adjacent epithelium. 2.HSP47 positive cells in connective tissue were mainly inflammatory cells, which is gradually increased from control to precancerous and cancer stage. But HSP47 positive cells after 14 weeks were decreased, especially normal and cancer adjacent epithelium. 3.The positive staining cells of HSP70 in control, dysplastic, and CIS stage were not seen. But they were mild findings in basal layer and moderate findings in spinous layer after experimental 14 weeks (cancer stage). 4.HSP70 positive cells were increased in precancerous and cancer stage than control group in connective tissue. After experimental 16 weeks, we could not find the HSP expression in cancer cells according to cancer differentiation or cancer stage. It is concluded that HSP70 or HSP47 expression is not a definitive marker of oral malignancy or malignant potential. However, with further development, HSP immunoreactivity may be valuable as an adjunct to conventional histology for assessing the malignant potential of oral mucosal lesions.