Effects of Protaetia brevitarsis seulensis powder dietary inclusion level on antioxidant activities of broiler breast meat during storage

Choi In‐Hag,Chung Tae‐Ho 한국곤충학회 2021 Entomological Research Vol.51 No.8

The present study aimed to evaluate the effects of Protaetia brevitarsis seulensis (PBS) powder dietary inclusion levels on the antioxidant activities of broiler breast meat during storage. A total of 270 Arbor Acres broiler chicks were randomly allocated to three treatments (3 replicates with 30 birds per pen), fed diets with inclusion of 0%, 0.5% or 1% PBS powder, in a completely randomized design. After slaughtering, samples were packed and stored at 4°C for later analysis of broiler breast meat. Antioxidant analysis included measurement of pH, thiobarbituric acid reactive substances (TBARS), and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. All samples were measured at storage days 0 and 7 as three replicates. For breast meat, changes were observed in pH, TBARS values, and DPPH radical scavenging at storage days 0 and 7 (p < 0.05), except for TBARS values at storage day 0. Overall, the addition of PBS powder resulted in lower TBARS values and higher DPPH radical scavenging in broiler breast meat during storage. Based on these results, it can be concluded that the addition of 0.5% and 1% PBS powder to broiler diets was effective at reducing lipid oxidation during storage, which could be related to the subsequent lower meat pH.

Antioxidant and cytoprotective effects of enzyme‐extracted constituents of Protaetia brevitarsis seulensis powder

Choi In‐Hag,Choi Sung‐Up,Son Jin‐Sung,Jang Woo‐Whan,Chung Tae‐Ho 한국곤충학회 2021 Entomological Research Vol.51 No.2

This study was aimed to compare the antioxidant and cytoprotective effects of hot‐water and enzyme‐extracted constituents of Protaetia brevitarsis seulensis powder. The products of enzymatic extraction had 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH)‐radical‐scavenging activity, cytotoxic effects, and provided cytoprotection against H2O2 and intracellular reactive oxygen species. Enzymatic extraction improved DPPH‐radical‐scavenging activity relative to hot‐water extraction (P < 0.05), with inhibition rates of 33% at 500 μg/mL and 35% at 1000 μg/mL. Cell viability ranged from 100 to 122%, and was higher for hot‐water than for enzymatic extraction (P > 0.05). Hot‐water extraction achieved greater cytoprotection against H2O2 than enzymatic extraction. However, enzymatic extraction achieved greater antioxidant and cytoprotective activity against reactive oxygen species than hot‐water extraction. These results provide evidence of the antioxidant and cytoprotective effects produced by enzymatic extraction of the active ingredients from Protaetia brevitarsis seulensis powder.

Utilization of Chemical Blends to Increase Nitrogen and Decrease Pathogens in Duck Litter

( In-hag Choi ) 한국환경과학회 2017 한국환경과학회지 Vol.26 No.10

This study examined the effects of chemical blends (a combination of alum and aluminum chloride) on pH, N, and pathogens in duck litter during a six-week experiment. In total, 240 Pekin ducks (160 males and 80 females) were individually distributed into 16 pens, in a randomized experimental design consisting of four treatments and four replicate pens per treatment. Our treatments included a control, T1 (75 g alum + 75 g aluminum chloride/kg duck litter), T2 (100 g alum + 100 g aluminum chloride/kg duck litter), and T3 (150 g alum + 150 g aluminum chloride/kg duck litter). There was no difference among treatments in pH and Total N (TN) at weeks 2, 4, and 6 and weeks 1, 4, 5, and 6, respectively. However, there were significant differences in both pH and TN among treatments at weeks 1, 3, and 5 and weeks 2 and 3, respectively. Regarding pathogens, we found small differences in all treatments in Escherichia coli populations from weeks 1 to 5 and in Salmonella enterica populations from weeks 1 to 3. In conclusion, the addition of chemical blends to duck litter increased TN, which resulted in a lower litter pH, but did not significantly affect pathogen populations.

깔짚에서 발생되는 가스와 휘발성지방산에 대한 황산알루미늄과 비교 시 혼합 홍삼박제제의 평가: 축산환경 경영관점에서

최인학 ( In Hag Choi ) 한국환경과학회 2015 한국환경과학회지 Vol.24 No.4

This study was conducted to determine the effects of mixed Korean red ginseng marc with aluminum sulfate on gas concentration and volatile fatty acid (VFA) in poultry litter during 4 weeks in terms of livestock and environment managements. A total of 240 broiler chicks were randomly allocated to four treatments in four replications and 15 birds per replicate. The four treatments was mixed to rice hull under each pen at 0, 10 g or 20 g red ginseng marc + 90g aluminum sulfate, and 100g aluminum sulfate per kg poultry litter (rice hulls). Carbon dioxide, methane, acetic acid, and propionic acids were measured weekly. The results that could be available include: First, during the experimental period, carbon dioxide emissions were not remarkably different among treatments. Second, no differences were observed among treatments in methane emissions at 2 weeks through 4 weeks, but at 1 week, the reduction in methane emissions was in following order: 100 g aluminum sulfate > 20 g red ginseng marc + 90 g aluminum sulfate > 10 g red ginseng marc + 90 g aluminum sulfate > control. Third, in spite of statistically differences, treatment with 10 g or 20 g red ginseng marc + 90g aluminum sulfate, and 100g aluminum sulfate reduced acetic acid and propionic acid as a function of time, except acetic acid in aluminum sulfate treatment at 2 and 4 weeks. In conclusion, the results indicated that like aluminum sulfate, using 10 g or 20 g red ginseng marc with aluminum sulfate was effective in decreasing methane and propionic acid released from poultry litter.

혼합 생균제 급여가 거세한우의 성장, 혈액성상 및 육질에 미치는 영향

( Dimas H. V. Paradhipta ),전충환 ( Chung-hwan Jeon ),최인학 ( In-hag Choi ),이혁준 ( Hyuk-jun Lee ),주영호 ( Young-ho Joo ),이성신 ( Sung-shin Lee ),김동현 ( Dong-hyeon Kim ),김삼철 ( Sam-churl Kim ) 한국환경과학회 2017 한국환경과학회지 Vol.26 No.8

In this study, the effect of probiotic supplementation on growth performance, blood metabolites, and meat quality of Hanwoo steer was investigated. A total of 32 Hanwoo steers (15-17 months, average body weight 462±37.9 kg) were randomly allotted to 4 dietary treatments (0, 0.5, 1.0, and 1.5% mixed probiotics), with four Hanwoo steers per pen (two replicates per treatments), and reared for 12 months. There were no differences among treatments in growth performance of Hanwoo steer (P>0.05); however, feed intake decreased linearly with increasing levels of mixed probiotics. Growth hormone and Blood Urea Nitrogen (BUN) levels responded linearly with increasing levels of dietary mixed probiotics (P<0.05), but not insulin and blood glucose did not. In particular, total cholesterol was significantly lower for the 1% mixed probiotic treatment in comparison with that of the other treatments (P<0.05). The pH, Thiobarbituric Acid Reactive Substances (TBARS), cooking loss, and meat color were influenced by increasing levels of mixed probiotics (P<0.05), but the carcass characteristics and shear force were not. Regarding sensory evaluation, the addition of mixed probiotics resulted in significant difference in meat color, tenderness, aroma, off-flavor, juiciness, and marbling score, but not in overall acceptability. In addition, fatty acid profiles indicated no differences between control and mixed probiotic treatments. In conclusion, mixed probiotic treatment at 1% levels can enhance consumer preferences possibly by reducing cholesterol and TBARS.

Alveolar ridge preservation with an open-healing approach using single-layer or double-layer coverage with collagen membranes

Choi, Ho-Keun,Cho, Hag-Yeon,Lee, Sung-Jo,Cho, In-Woo,Shin, Hyun-Seung,Koo, Ki-Tae,Lim, Hyun-Chang,Park, Jung-Chul Korean Academy of Periodontology 2017 Journal of Periodontal & Implant Science Vol.47 No.6

Purpose: The aim of this prospective pilot study was to compare alveolar ridge preservation (ARP) procedures with open-healing approach using a single-layer and a double-layer coverage with collagen membranes using radiographic and clinical analyses. Methods: Eleven molars from 9 healthy patients requiring extraction of the maxillary or mandibular posterior teeth were included and allocated into 2 groups. After tooth extraction, deproteinized bovine bone mineral mixed with 10% collagen was grafted into the socket and covered either with a double-layer of resorbable non-cross-linked collagen membranes (DL group, n=6) or with a single-layer (SL group, n=5). Primary closure was not obtained. Cone-beam computed tomography images were taken immediately after the ARP procedure and after a healing period of 4 months before implant placement. Radiographic measurements were made of the width and height changes of the alveolar ridge. Results: All sites healed without any complications, and dental implants were placed at all operated sites with acceptable initial stability. The measurements showed that the reductions in width at the level 1 mm apical from the alveolar crest (including the bone graft) were $-1.7{\pm}0.5mm$ in the SL group and $-1.8{\pm}0.4mm$ in the DL group, and the horizontal changes in the other areas were also similar in the DL and SL groups. The reductions in height were also comparable between groups. Conclusions: Within the limitations of this study, single-layer and double-layer coverage with collagen membranes after ARP failed to show substantial differences in the preservation of horizontal or vertical dimensions or in clinical healing. Thus, both approaches seem to be suitable for open-healing ridge preservation procedures.

어성초를 오리사료에 첨가 시 오리깔짚의 특성 비교

최인학 ( In Hag Choi ) 한국환경과학회 2017 한국환경과학회지 Vol.26 No.2

The objective of this study was to evaluate the characteristics of duck litter in ducks fed diets containing Houttuynia cordata powder. One-day-old ducklings (Pekin) were randomly divided into two groups and fed a control or 1% H. cordata powder-containing diet for an experimental period of three weeks. The results showed that pH for 1-2 weeks and total nitrogen for 1-3 weeks in duck litter were affected by dietary treatments with 1% H. cordata powder (P < 0.05). For Volatile Fatty Acids (VFAs), there was no significant difference (P > 0.05) between ducks fed 1% H. cordata and control diets, as shown in the results for acetic acid and propionic acid in duck litter over three weeks; but, this was not the case for propionic acid at 3 weeks. The inclusion of 1% H. cordata powder in the diet had a the positive effect on increasing the total nitrogen and decreasing pH and VFAs in duck litter.

사육방식에 따른 계란의 지방산 조성: 현장연구를 중심으로

최인학 ( In Hag Choi ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.6

This study examined the effects of cage rearing system and backyard raising system on fatty acid profile of egg in laying hens during 9 weeks. Two hundred hens, 70-wk-old, Hy-line Brown, were used in this study and fed basal diets. 100 hens, randomly collected from facility, were transferred to cage rearing system and 100 remaining hens were moved to backyard raising system. For overall period, higher unsaturated fatty acid percentages in two different rearing systems than did saturated fatty acid (SFA) were observed. In unsaturated fatty acid compositions, both rearing system showed a higher mono unsaturated fatty acid (MUFA) contents than poly unsaturated fatty acid (PUFA) contents. Egg quality traits of laying hens reared in backyard system had considerably lower contents of SFA and higher contents of MUFA compared with eggs from cage rearing system (P<0.05). In addition, eggs from cage rearing system resulted in a significantly lower n-3 contents and higher n-6:n-3 ratios and CLA contents in comparison with backyard raising system (P<0.05). However, no significant effects of two different rearing systems on PUFA, PUFA:SFA ratio, and n-6 contents were observed during the experimental period. In conclusion, this result suggests that cage rearing system and backyard raising system will play an important role in improving egg production as functional foods.

연구노트 : 케이지 사육에서 방사사육으로 전환한 산란노계의 가슴살과 대퇴부살에 포함된 지방산 함량의 비교 조사

김창만 ( Chang Man Kim ),최정훈 ( Jung Hoon Choi ),최인학 ( In Hag Choi ) 한국환경과학회 2012 한국환경과학회지 Vol.21 No.6

The objective of this study was undertaken to investigate changes in fatty acid profile of breast and thigh muscle of old laying hens as transferred from cage to backyard raising system and to provide basic information on using old laying hens to producers for environmental managements. Overall, percentages of unsaturated fatty acid obtained from breast and thigh muscle with cage and backyard raising system are the most abundant, followed by saturated fatty acid. For breast muscle, there were no differences (P>0.05) between cage rearing system and backyard raising system in saturated fatty acid (SFA) and mono unsaturated fatty acid (MUFA). Poly unsaturated fatty acid (PUFA) and PUFA: SFA ratios were significantly higher in backyard raising systems in comparison with cage rearing systems (P<0.05). No differences (P>0.05) were detected between both rearing systems for MUFA, PUFA, and PUFA: SFA ratios with thigh muscles. SFA in thigh muscle was significantly (P<0.05) higher in cage rearing systems compared with backyard raising systems. In conclusion, using breast and thigh muscle meats from old laying hens as functional foods or investigating impact assessment on the improvement of farm management system may serve as a good management practice to encourage producers.

사료와 축분에 생균제 첨가 시 한우분 내 질소와 수용성인의 함량변화: 현장연구 중심으로

최인화 ( In Hag Choi ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.3

This study was conducted to investigate the changes in nitrogen and soluble reactive phosphorus(SRP) contents from Hanwoo manure using probiotics to feed and manure additives during 5 weeks, A total of 45hanwoo(24 menths old) with Averaging 580 ±20kg in weight were randomly assigned to 3 dietary treatments with 3 replicates per treatment(5 hanwoo per pen, 5×8m). The treatment were supplemented control, T1(10kg roughage+2kg concentrate(2%probiotics as fed basis)). And T2(10kg roughage+2kg concentrate(2% probiotics as fed basis) + 7 kg probiotics on the surface of hanwoo manure (top dressing)). During the experimental period, there were statistically significant differences(P<0.05) in pH values at 3 and 5 weeks; TN contents at 5 weeks; and SRP contents at 5 weeks in all treatments. Adding probiotics to feed or feed and manure increased manure pH in comparison with controls. As time increased, changes in TN contents decreased in the order; T2>Control> T1. Especially,the reduction in SRP contents in all treatments at 5 weeks was in following order; T1>T2>Control. This result suggests that it is possible to make efficient use of probiotics as feed and manure additives for reducing environmental pollution or to provide fundamental information on livestock managements to producers.