피부 병변을 동반한 악성조직구증 1예

조은택,박용관,김진호,강정원,천영욱,전익섭,박유환,전춘해 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1

Malignant histiocytosis, originally described in 1939 as histiocytic medullary reticulosis by Scott and Robb-Smith, is a rare histiocytic proliferative disorder that often shows an acute onset and used to progress to death within a few months. This disorder characterized clinically by fever, generalized weakness, lymphadenopathy and hepatosplenomegaly, and shows a variable range of pancytopenia. Extranodal involvement is common, with an incidence ranging from 50% to as high as 90%, skin involves8ment was noted in 10% to 15%. Typical skin lesions were mainly founded in extremity. i.e. erythematous papule and nodule occasionally become to necrosis and ulceration. We experienced one case of malignant histiocytosis in a 46-years-old female. The major clinical findings are fever, malaise, hepatosplenomegaly and erythematous skin lesion. In the laboratory study, pancytopenia is noted on the peripheral blood. And also aggregation of many atypical histiocytes were shown on skin and bone marrow biopsy. So we reported one related case with malignant histiocytosis as well as reviewing literature .

골분을 이용한 구리 흡착 첨착

김은정,박정훈,김정섭,유현철,이봉섭,곽명화,우성훈,박승조 東亞大學校 環境問題硏究所 2005 硏究報告 Vol.27 No.1

Spent pig bone was consist of hydroxyapatite. And then we thought that it have a possibility as a adsorbent. The preparation of bone char prepared from spent pig bone in this study. We study on the recycling possibility as a adsorbent is able to adsorb copper ion from their single aqueous solutions. The results are summarized as follows. Bone char that prepared from spent pig bone at 105, 400, 700℃. The adsorption removal efficiency of copper ion onto bone char as adsorbent was above 90% for 15 hours. Increase in the initial pH of the copper ion solution result in an increase in the copper ion uptake per unit weight of the adsorbent. Freundlich isotherm model was found to be applicable for the experiment data of copper ion. The results showed that spent pig bone can be used for the absorption of the copper ion.

2004년 7월 봇돌바다의 썰물, 전류 및 밀물시 수온과 염분 분포

최용규,조은섭,이용화,이영식 한국환경과학회 2005 한국환경과학회지 Vol.14 No.2

Based on the observation on 20, 23 and 26 July 2004, the distributions of temperature, salinity and stratification was investigated in relation to ebb, turn of tide and flood. The results are as follows: 1) The high temperature and low saline water with 23.5-24.0℃ and 32.4~33.0psu existed at Naro Island. 2) The cold surface water below 21.00 and 33.0~33.4psu appeared in the area near Gae Island and Geumo Island. 3) The cold and saline water, below 24.0℃ at the surface and 17.0℃ near the bottom, 32.8~33.8psu at the surface and 33.8~34.0psu near the bottom, existed in Sori Island. These waters were more saline compared to the South Sea Coastal Water with about 31.8psu. This suggests that the oceanic saline water intruded into the Bottol Bada through the area near Sori Island. The stratification appeared during all the observation periods due to a high solar radiation of 22MJ/m², and a weak wind speed of 2.9m/s on the average while the mean speed of wind in July is around 3.9 m/s. It qualitatively suggested that the stratification was maintained during the observation periods because of a high solar radiation, a weak wind speed and intrusion of saline oceanic water.

2004년 7월 봇돌바다의 영양염과 Chlorophyll-a의 단기 변동

최용규,조은섭,권기영,이용화,이영식 한국환경과학회 2005 한국환경과학회지 Vol.14 No.4

In order to study the temporal variations of nutrients and chlorophyll-a in the Bottol Bada, three field observations were carried out on 20, 23 and 26 July, 2004. The low N:P values exhibit nitrogen deficiency during the periods of observation. This result is not representative of typical summer environment in the southern coast of Korea. The possible mechanisms are as follows: 1) The freshwater inflow was not sufficient for the supply of nitrogen because the total precipitation was 11.9 mm in July, 2004. This amount is no more than 5% in normal precipitation in July. 2) There was an inflow of oceanic water under the subsurface into the Bottol Bada. Even though the oceanic water comprises more nutrients, it produces the stratification between the surface and the subsurface water and seems to prevent the supply of nutrinets to the surface layer. 3) The high chlorophyll-a concentration of 1.2 ㎍/L was shown near the narrow channel between Gae-do and Geumo-do. This seems to be resulted from the inflow of water from Gamak Bay.

The Population Genetic Structure of the Oyster Crassostera gigas (Bivalvia : Ostreidae) from Gamak Bay in Korea

Eun Seob Cho(조은섭),Hee Dong Jeong(정희동) 한국생명과학회 2008 생명과학회지 Vol.18 No.7

본 연구는 2007년 3월 가막만에서 양식하고 있는 참굴 34마리를 대상으로 미토콘드리아COI gene 염기서열분석을 통하여 유전자 집단을 조사했다. PCR 증폭에 사용된 primer는 HCO2918과 LCO1491로 product는 658 bp였다. 유전자 배열 결과 가막만 참굴 집단에서 CR1, CR2, CR3, CR4 4개의 haplotype이 나타났다. 그 중 CR3 haplotype이 73% 발생되어 가장 높은 빈도율을 보였다. 계층구조에서도 가막산 참굴은 남해안산과 유전적 유연관계를 보이나, 유전적 거리는 남해안 및 서해안과도 마이너스 값을 보이며 통계적으로도 유전적으로 하나의 집단을 형성하고 있음을 알 수 있다. To analyze the population genetic structure of the oyster Crassostrea gigas Thunberg, 34 specimens werecollected from Gamak bay in March, 2007. Total genomic DNA was extracted from each sample and PCR was performed to identify haplotypes of oyster by using HCO2918 and LCO1491 primers. Four kinds of haplotypes (CR1, CR2, CR3, and CR4) were identified. Among these group, CR3 showed the highest relative frequency at 73% than any other of haplotypes. On the basis of hierarchical genetic structure, the population of Gamak showed a higher genetic relationship with Namhae, but the genetic distance between southern and western coasts was negative and no statistical significance was found (p>0.05). Consequently, the oyster from Korea coast is determined to be both homogenous and large.

분자생물학적 방법을 이용하여 마비성 패류 독소를 생산하는 알렉산드륨 타마렌스 시스트 탐색

Cho, Eun-Seob Korean Society of Life Science 2003 생명과학회지 Vol.13 No.2

알렉산드륨 적조생물 속에서 마비성 패류독소를 생산하는 종을 신속하게 동정하므로 패류양식의 독성 모니터링과 방제에 중요한 역할을 할 수 있다. 자연상태에서 영양세포가 출현하기 전 알렉산드륨 타마렌스의 휴면포자만을 신속하게 분리 동정한다는 것은 근본적인 마비성 패류독소 모니터링 및 예측에 큰 역할을 할 수 있다. cTAM-Fl DNA probe은 알렉산드륨 타마렌스의 영양세포 뿐만 아니라 primuline으로 염색하여 메타놀로 고정한 휴면포자에도 반응이 되었다. 영양세포와 휴면포자에 반응되는 DNA probe 위치는 핵내의 말단 부위에 보였다. DNA probe가 세포내로 삽입되는데 가장 적합한 온도와 시간은 50-$54^{\circ}C$, 40-60분이 좋았다. Identification of species within the toxin-producing genus Alexandrium is vital for biotoxin monitoring and mitigation decisions regarding shellfish industry. In particular, the discrimination of resting cysts of only A. tamarense from that of Alexandrium spp. is considerable important to fundamentally monitor and predict this species before vegetative cells occur in the nature. Fluorescent cTAM-F1 DNA probe was responsible to not only binding the activity of the vegetative cells in A. tamarense, but also to the resting cysts, which was treated with methanol after fixation and stained by primuline on the surface The location of fluorescence in cultured vegetative cells and resting cysts was almost at tile bottom of the nucleus. The optimal incubation temperature and time using in situ hybridization were 50-$54^{\circ}C$ and 40-60 min, respectively, to penetrate the DNA probe into cell.

Phylogenetic Relationship among Several Korean Coastal Red Tide Dinoflagellates Based on their rDNA Internal Transcribed Spacer Sequences

Cho, Eun-Seob,Kim, Gi-Yong,Park, Hyung-Sik,Nam, Byung-Hyouk,Lee, Jae-Dong Korean Society of Life Science 2001 Journal of Life Science Vol.11 No.2

The nucleotide sequences of the internal transcribed spacer regions (ITS1 and ITS2) of ribosomal DNA (rDNA), and the 5.85 rRNA gene, have been determined for 13 strains of dinoflagellates in order to analyze the phylo-genetic relationship. The DNA sequences contained considerable variation in the ITS regions, but little in the 5.85 rDNA. In addition, the ITS1 was more variable than the ITS2 in all species examined. The nucleotide length of this region varied from 519 bp to 596 bp depending on the taxa. The investigated taxa were divided into three large groups based on the ITS length, i. e., a group with short ITS region (A. fraterculus and Alexandrium sp.), a with ITS region group (P. micans, P. minimum and P. triestinum) and a with ITS region group (G. impudicum, C. polykrikoides, G. sanguineum, G. catenatum and H. triquetra). The relationship between nucleotide length of ITS1 and that of ITS2 was negative, whereas G+C content and nucleotide length showed positive correlation. In phylogenetic analyses producing NJ trees, the topology was similar cluster and clearly divided the taxa into three groups based on 5.8S rDNA that were similar to those based on morphological characteristics. In particular, G. impudicum was more closely related to G. catenatum than to C. polykrikoides using phylogenetic analysis. From this study, we chew that the length of ITS region contributes to discriminate Korean harmful algal species and ITS analysis is a useful method for resolving the systematic relationships of dinoflagellates.

ITS2 부위의 염기서열 및 RAPC-PCR에 의한 Pseudo-nitzschia 4종의 유연관계

Cho, Eun-Seob,Lee, Young-Sik Korean Society of Life Science 2004 생명과학회지 Vol.14 No.1

ITS2 부위를 시퀀싱하여 Pseudo-nitzschia delcatissima, P. multiseries, P. pungens, P. subfraudulenta 상호간의 유전자 다양도를 조사함과 아울러 RAPD-PCR pattern을 이용하여 유사도를 구하였다. 유전자 거리를 근거로 했을 때 P. delicatissima 종은 P. multiseries와 P. pungens와는 유전적 거리가 상당히 요원하였고, 심지어 P. subfraudenlta와도 거리를 보였다. 유사도의 경 P. multiseries와 P. pungens는 0.31로 보인 반면에, P delicatissima는 다른 세종과 0.81를 나타내었다. 따라서 P. delicatissima 종은 P. multiseries, P. pungens, P. subfraudulenta와는 유전적으로 밀접하지 않는 관계로 보였다. ITS2부위는 Pseudo-nitzschia 동정에 사용될 수 있는 유용한 도구로 보이며 형태적으로 구분할 수 없는 P. multiseries와 P. pungens을 구분할 수 있다. 또한 RAPD-PCR 방법도 단시간에 Pseudo-nitzchia을 분리시키는데 사용될 것으로 보인다. A portion of ribosomal internal transcribed spacer (ITS) 2 was sequenced from the samples of Pseudo nitzschia (P. deticatissima, P. multiseries, P. pungens and P. subfraudulenta) to investigate the genetic characteristics by measuring tile magnitude of genetic diversity and the degree of similarity coefficient using random amplified polymorphic DNAs (RAPD)-PCR patterns. The phylogenetic trees inferred from the genetic distance analyses showed the placement of P. delicatissima formed a quite long distance from p. P. multiseries, P. pungens, and even P. subfraudulenta. The phylogenetic tree from RAPD patterns showed that P. multiseries and P. pungens had dissimilarity coefficient of 0.31, while P. delicatissima and three species of Pseudo-nitzschia had that of 0.81. It is likely thought that the genetic position of P. delicatissima formed far from P. multiseries, P. punges, and P. subfraudulenta. These results imply that ITS2 region is expected to support a useful molecular characters for recognizing at the species level and for even discriminating P. multiseries from P. pungens. RAPD method also will be used to differentiate the species of Pseudo-nitzschia in a short time.

Dependence of Sub-Cellular Activities of the Blooming and Harmful Dinoflagellate Cochlodinium Polykrikoides on Temperature

Eun Seob Cho(조은섭) 한국생명과학회 2008 생명과학회지 Vol.18 No.9

본 연구는 유해성 Cochlodinium polykrikoides 적조생물을 대상으로 수온변화에 따른 세포 생화학적 및 생리 활성도를 측정했다. Genomic DNA 함량은 12℃ 및 15℃에서 거의 비슷한 0.6을 보였으나, 18℃부터 급격히 높아져서 24℃ 최고 1.8를 나타내었다. RNA와 total protein도 24℃에 가장 높은 1.7과 0.07 ㎍ ml?¹으로 나타났다. 광합성량도 수온에 따른 큰 변화를 보였다. 빛의 파장에 관계없이 18℃ 이상에서 현저히 높은 값을 보였다. 24℃ ETRmax Ch1-Ch4까지의 범위는 537.9에서 602.5 μ㏖ electrons g?¹ Chl α s?¹ 나타났다. Nitrate reductase와 ATPase 효소 활성도는 24℃에서 각각 0.11 μ㏖이 NO₂? ㎍?¹ Chl α h?¹, 0.78 pmol 100 ㎎?¹ 나타났다. CHN 분석에서도 수온에 따라 C, H, N의 함량이 현저하게 상이했다. 27℃ 배양시 24℃에 비하여 대부분의 세포생리물질이 낮게 보였다. 따라서 C. polykrikoides는 수온 변화에 대하여 세포대사물질의 함량이 많은 차이를 볼 수 있어서 초기 적조 발생 조건은 18℃로 추측된다. 본 실험의 결과로 24℃ 이상이 되면 C. polykrikoides 대번식은 세포 내 생리물질의 현저한 저하로 형성되기가 어려울 것으로 보인다. Water temperature-dependent fluctuations of biochemical and molecular activities in the harmful dinoflagellate, Cochlodinium polykrikoides were studied. In terms of genomic DNA concentration, a similar value of 0.6 was observed at 12℃ and 15℃. However, DNA significantly increased beyond 18℃ (p<0.05), to a maximum of 1.8 at 24℃. DNA concentration significantly decreased to 0.6. The concentrations of RNA and total protein were likely at their highest values of 1.7 and 0.07 ㎍ ml?¹ at 24℃, respectively. RNA and total protein concentrations began to increase at 15℃. Oxygen availability between lower and higher temperatures was significantly different and increased from 18℃ according to light intensity, regardless of wavelengths (p<0.05). At 24℃, the highest value of the maximum electron transport rate (ETRmax), ranging from 537.9 (Ch 1) to 602.5 μ㏖ electrons g?¹ Chl α s?¹ (Ch 4), was also apparent. Nitrate reductase (NR) and ATPase activities were at their highest values of 0.11 μ㏖ NO₂?㎍?¹ Chl α h?¹ and 0.78 pmol 100 ㎎?¹ at 24℃, respectively. In an analysis of CHN, the concentration of C and N also significantly increased (p<0.05). Most of the measurements for the cellular activities at 27℃, however, were less than at 24℃. These results suggest that the sub-cellular activities of C. polykrikoides are sensitive to changes in water temperature. It may be desirable to estimate at 18℃ the initiation of the massive blooming development of C. polykrikoides. In nature, it will be very difficult to maintain the massive blooms beyond 24℃ because of a possibly significant decrease in molecular activity of C. polykrikoides.

Genetic Responses of the Ark Shell Scapharca broughtonii Schrenck to Environmental Shock: High Temperatures and Long Exposure Times

Cho, Eun-Seob,Jung, Choon-Goon,Shin, Yun-Kyung 한국해양학회 2009 Ocean science journal Vol.44 No.2

Genetic responses of non-high temperature exposed and high temperature exposed ark shell (Scapharca broughtonii) cells collected from Dukyang Bay in August 2007 to environmental shock have been compared. In the analysis of two-dimensional electrophoresis, seven protein spots were found. They have no matching protein in the database, but the number of protein (#1) in unstressed ark shells differed from that of stressed ark shells, indicating a strong signal on the gel and had a homology with arginine kinase (molecular weight, 38 kDa; pI 8.3). The predicted tertiary structure of arginine kinase formed finger-like antiparalled b-sheet projections. A small a-helical N-terminal domain is followed by a larger C-terminal domain. Extracted total RNA was reverse transcribed and amplified by PCR with primer used in this study. Mantle, gill and visceral in non-high temperature exposed ark shell showed some levels of arginine kinase transcripts, but not the foot. These cells also showed amplified fragments on the gel exposed to $20^{\circ}C,\;23^{\circ}C$ and $27^{\circ}C$, but the foot cell had no expression of arginine kinase gene at $27^{\circ}C$ for 3 hrs. These findings provide important insights into the possible molecular mechanisms in which no expression of arginine kinase is contributed to preventing the binding of arginine and actin and difficult to move foot muscle.