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Yun, Cheol-Heui,Son, Chang Gue,Chung, Dae Kyun,Han, Seung Hyun 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.24 No.-
Chlorophyllin (CHL) is a chlorophyll derivative with anticarcinogen and antioxidant activities. Despite clinical importance of CHL as a potential therapeutics for treating cancer patients, little is known about the immunological properties of CHL. In the present study, we investigated the effect of CHL on the activation of murine splenocytes stimulated with lipopolysaccharide (LPS). RT-PCR analysis showed that LPS-activated IFN-γ expression gradually declined by CHL treatment in a dose dependent manner while mRNA production of TNF-α, IL-2, and FasL was not changed. CHL also suppressed IL-12 production (p70, a heterodimer of p40 and p35) and the mRNA expression of IL-12 p40 and IL-12 receptors (both IL-12Rβ1 and IL-12Rβ2), which are involved in the induction of IFN-γ expression. Furthemore, an electrophoretic mobility shift assay showed that CHL inhibited DNA binding activity of NF-кB, STAT-3, and STAT-4 to their cognate DNA recognition motifs, all of which contribute to the IL-12-induced IFN-γ transcription. Exogenous addition of recombinant IL-12 abrogated the inhibitory effect of CHL on IFN-γ and its mRNA expression in LPS-activated splenocytes. Collectively, these results show that CHL inhibits IFN-γ production by LPS-stimulated splenic mononuclear cells due to down-regulation of IL-12 production. © 2005 Published by Elsevier B.V.
Cheol Gyun Kim,Won Kyong Kim,Narae Kim,Young Jin Pyung,Da-Jeong Park,Jeong-Cheol Lee,Chong-Su Cho,Hyuk Chu,Cheol-Heui Yun 대한면역학회 2023 Immune Network Vol.23 No.6
Scrub typhus, a mite-borne infectious disease, is caused by Orientia tsutsugamushi. Despite many attempts to develop a protective strategy, an effective preventive vaccine has not been developed. The identification of appropriate Ags that cover diverse antigenic strains and provide long-lasting immunity is a fundamental challenge in the development of a scrub typhus vaccine. We investigated whether this limitation could be overcome by harnessing the nanoparticle-forming polysorbitol transporter (PST) for an O. tsutsugamushi vaccine strategy. Two target proteins, 56-kDa type-specific Ag (TSA56) and surface cell Ag A (ScaA) were used as vaccine candidates. PST formed stable nano-size complexes with TSA56 (TSA56-PST) and ScaA (ScaA-PST); neither exhibited cytotoxicity. The formation of Ag-specific IgG2a, IgG2b, and IgA in mice was enhanced by intranasal vaccination with TSA56-PST or ScaA-PST. The vaccines containing PST induced Ag-specific proliferation of CD8+ and CD4+T cells. Furthermore, the vaccines containing PST improved the mouse survival against O. tsutsugamushi infection. Collectively, the present study indicated that PST could enhance both Ag-specific humoral immunity and T cell response, which are essential to effectively confer protective immunity against O. tsutsugamushi infection. These findings suggest that PST has potential for use in an intranasal vaccination strategy.