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- 저자 : Chenzhong Li (검색결과 3 건)
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Shikha Kumari,Abha Mishra,Divakar Singh,Chenzhong Li,Pradeep Srivastava 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.1
This research study deals with the development of copper nanoparticles (CN) and nano-hydroxyapatite (nHAP) infused chitosan (C) and gelatin (G) based nanocomposite scaffolds for bone tissue engineering applications. Human-origin osteoblast cells (MG-63) were seeded over the scaffolds to investigate the novel biomimetic extracellular matrix system. The scanning electron microscopy (SEM) showed an average pore size between 100-146 μm for all the C-G-nHAP-CN based scaffolds. The in-vitro degradation study showed 74-83% degradation for CN-based scaffolds. For 0.03% CN based scaffold degradation rate (84%) was very close to the control scaffold. Swelling ratio was highest for the chitosan scaffold and it was in the range between 5.25-5.93 mg/mL for other scaffolds. Compressive moduli were highest for 0.03% CN scaffold (3.32 MPa) which was relatively very high in comparison to C-G-nHAP scaffold with 2.4 MPa strength in a wet state. Stress-strain graphs also show the maximum displacement by 0.03% CN scaffold. The functional and structural analysis for the scaffolds showed the presence of nHAP in the scaffold and CN peaks within the composite structure. Differential scanning colorimetry testing showed reduced crystallinity in CN-based scaffolds with a melting temperature of 320ºC. Their 2D cell behaviour in the Electrical Cell Impedance System (ECIS) study showed maximum cell spreading and growth in 0.02% CN-based scaffold. The cell-seeded composite was tested for 3-(4,5-dimethylthiazolyl-2)-2,5- diphenyltetrazolium bromide (MTT), 4,6-diamidino-2- phenylindole (DAPI), and acridine orange and propidium iodide (AOPI) assay for testing its cytocompatibility for MG-63 cell line. Cell proliferation and cell spreading was observed by SEM in all the CN-based scaffolds. Alkaline phosphatase (ALP) activity was highest in 0.03% CN scaffold with 2.0 optical density (OD) value. Alizarin Red Stain (ARS) staining was performed to support this study. It can be statistically depicted that nHAP and 0.03% CNbased scaffold could be potential biomaterial for minor to severe bone-related tissue regeneration applications.
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Tizoxanide induces autophagy by inhibiting PI3K/Akt/ mTOR pathway in RAW264.7 macrophage cells
Jiaoqin Shou,Mi Wang,Xiaolei Cheng,Xiaoyang Wang,Lifang Zhang,Yingchun Liu,Chenzhong Fei,Chunmei Wang,Feng Gu,Feiqun Xue,Juan Li,Keyu Zhang 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.2
As the main metabolite of nitazoxanide, tizoxanide(TIZ) has a broad-spectrum anti-infective effect againstparasites, bacteria, and virus. In this study, we investigatedthe effects of TIZ on autophagy by regulating the PI3K/Akt/mTOR signaling pathway. RAW264.7 macrophage cellswere treated with various TIZ concentrations. Cell viabilityassay, transmission electron microscope, and immunofluorescencestaining were used to detect the biological functionof the macrophage cells, and the expression levels of theautophagy pathway-related proteins were measured by Westernblot. Results revealed that TIZ promoted the conversionof LC3-I to LC3-II, the formation of autophagy vacuoles,and the degradation of SQSTM1/p62 in a concentration- andtime-dependent manner in RAW264.7 cells. Treatment withTIZ increased the Beclin-1 expression level and inhibitedPI3K, Akt, mTOR, and ULK1 activation. These effects wereenhanced by pretreatment with rapamycin but attenuated bypretreatment with LY294002. In addition, the conversion ofLC3-I to LC3-II was observed in Vero, 293T, and HepG2cells treated with TIZ. These data suggested that TIZ mayinduce autophagy by inhibiting the Akt/mTOR/ULK1 signalingpathway in macrophages and other cells.
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