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        Integrated Metabolome and Transcriptome Analysis Reveal Complex Molecular Mechanisms Underlying Selenium Response of Aloe vera L.

        Zou Yi,Changyou Han,Fang Wang,Yanhua Tan,Saina Yang,Chuying Huang,Shengyou Xie,Xueqin Xiao 한국식물학회 2021 Journal of Plant Biology Vol.64 No.2

        Aloe vera L. is an excellent resource for medication. Selenium-enriched aloe can act as a functional food to human health. To understand the molecular mechanisms underlying selenium accumulation in Aloe vera L., we characterized the metabolic and transcriptome responses of aloe leaves under different Na2SeO4 levels (0, 200, and 400 mg/L) treatments. Aloe leaves spraying with exogenous selenium fertilizer showed a significant increase in total Se content compared with those under non-treatment control, and no distinct differences were observed between 200 and 400 mg/L Se treatment. Non-targeted metabolic profiling revealed that Se treatment triggered the accumulation of antioxidants, including amino acid and derivatives, phenols, flavonoids, terpene, as well as indole derivatives. Consistent with metabolic changes following Se treatment, the transcript level of genes involved in Se assimilation and Se-response showed dramatically increase, such as those encoding sulfate transporter, antioxidants, phytohormone signaling, transcription factors, and phenols metabolites, suggesting Se assimilation generally accompanied with antioxidant and pathogen defense. This study exhibited comprehensive insights on Se response in Aloe vera L., and provided us with targeted genes for genetic engineering, thereby improving the therapeutic value of aloe.

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        Reinforcement of Waterborne Polyurethane Films with Poly(acrylic acid)-Modified Palygorskite Fibers

        Yu Mao,Lingli Ni,Changyou Yang,Peng Cai,Weigang Du,Xiaoyan Gao 한국섬유공학회 2020 Fibers and polymers Vol.21 No.9

        Palygorskite (PAL) is a natural fibrous clay mineral which attracted tremendous attention as reinforcing agent topolymers. In this paper, a facile and environmental friendly modification process of PAL by poly acrylic acid (PAA) via insitupolymerization in PAL/water gel has been reported. The effects of PAA modified PAL (PAA-PAL) on the mechanical andthermal properties of waterborne polyurethane (WPU) nanocomposites have been investigated. Scanning electronmicroscopy (SEM) demonstrated that the dispersion of PAL has improved dramatically after PAA modification. Tensile testsshowed that PAA-PAL has a significant reinforcement effect on WPU matrix. Addition of 10 wt% PAA-PAL, the tensilestrength and the Young’s modulus of WPU composites increased 235 % and 388 %, respectively. Furthermore, the thermalstability of WPU also has been distinctly improved via addition of PAA-PAL.

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        Sensitivity analysis and parameter interval optimization for residual stress in polishing process of GH4169 blisk blade

        Xiaojun Lin,Xiaopeng Xin,Rui Yang,Zhaozhao Lei,Luzhou Sun,Bao Yang,Changyou Bai,Yangwei Yan 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.2

        Surface integrity has a significant impact on the performance and life cycle of the blisk blade. Residual stress, as one of the important characterization parameters of surface integrity, is closely related to the performance of the aeroengine blisk blade. A certain interval of residual compressive stress can significantly inhibit the generation of microcracks. This paper proposes an optimization method of polishing process parameter interval based on sensitivity analysis. This research studied the effect of polishing parameters of the alumina ACW (abrasive cloth wheel) on the surface residual stress of the GH4169 superalloy blisk blade. Firstly, the experiment was designed by four factors and three levels response surface method, the multiple regression of the experimental data based on Minitab software was used to get a prediction model of polished surface residual stress. And an analysis of variance was performed on the model. Subsequently, the model of relative sensitivity was got based on this model. The sensitivity order of polishing surface residual stress to each polishing parameter was obtained. And the judging method of stable interval and instable interval was put forward. Then, a parameter interval optimization method was proposed, and the optimal interval of process parameters was obtained. Finally, a polishing verification experiment was performed. The results show that: for the polishing of GH4169 superalloy blades, the surface residual stress is most sensitive to the feed rate vf of the ACW, followed by the granularity p and the rotation rate ω, and the compression depth ap is the least sensitive. The optimal interval of polishing process parameters is: the rotation rate ω is [11000 r/min, 11500 r/min], the feed rate vf is [100 mm/min, 200 mm/min], the compression depth ap is [0.6 mm, 1.0 mm], and the granularity p is [400 #, 600 #]. The optimized polishing process parameters can obtain a large and stable surface residual compressive stress, which is helpful to increase fatigue strength of the blisk blade.

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