Urinary concentration of transforming growth factor-&bgr;-inducible gene-h3(&bgr;ig-h3) in patients with Type 2 diabetes mellitus

Cha, D. R.,Kim, I. S.,Kang, Y. S.,Han, S. Y.,Han, K. H.,Shin, C.,Ji, Y. H.,Kim, N. H. Blackwell Science Ltd 2005 Diabetic medicine Vol.22 No.1

<P>Abstract</P><P>Aims </P><P>The expression of TGF&bgr;-inducible gene h3(&bgr;ig-h3) has been used to assess the biological activity of TGF&bgr; in the kidney. In this study, we investigated whether the urinary concentration of &bgr;ig-h3 is associated with diabetic nephropathy in patients with Type 2 diabetes mellitus. We also evaluated the relationship between the urinary concentration of &bgr;ig-3 and proteinuria and microalbuminuria (AER) in a normal healthy population and in Type 2 diabetes patients.</P><P>Methods </P><P>Four hundred and seventy-nine Type 2 diabetic patients without non-diabetic kidney diseases and 528 healthy control subjects were enrolled. The study subjects were divided into five groups: a non-diabetic healthy control group with normal ACR (<I>n</I> = 443), a non-diabetic healthy control group with microalbuminuria (<I>n</I> = 85), a normoalbuminuric diabetic group (<I>n</I> = 198), a microalbuminuric diabetic group (<I>n</I> = 155) and an overt proteinuria group (<I>n</I> = 126). Urinary levels of &bgr;ig-h3 were measured by enzyme-linked immunosorbent assay.</P><P>Results </P><P>(i) Urinary excretion of &bgr;ig-h3 was significantly higher in the diabetic groups than in the controls, even in the normoalbuminuric stage (25.02 ± 8.84 vs. 18.67 ± 6.56, <I>P</I> = 0.03). In diabetic patients, urinary &bgr;ig-h3 levels increased significantly as diabetic nephropathy advanced (25.02 ± 8.84 vs. 34.06 ± 24.55 vs. 169.63 ± 57.33, <I>P</I> < 0.001). (ii) Proteinuria was found to be significantly correlated with urinary &bgr;ig-h3 (healthy control; <I>r</I> = 0.137, <I>P</I> = 0.019, diabetic patients; <I>r</I> = 0.604, <I>P</I> < 0.001). ACR was also found to be significantly related with urinary &bgr;ig-h3 in diabetic patients (<I>r =</I> 0.383, <I>P</I> = 0.006). (iii) In diabetic patients, urinary &bgr;ig-h3 was significantly related with systolic and diastolic blood pressure (systolic blood pressure: <I>r</I> = 0.436, <I>P</I> = 0.024; diastolic blood pressure, <I>r</I> = 0.365, <I>P</I> = 0.042), total cholesterol and HbA<SUB>1c</SUB> (cholesterol: <I>r</I> = 0.169, <I>P</I> = 0.03, HbA<SUB>1c</SUB>; <I>r</I> = 0.387, <I>P</I> = 0.044). Logistic regression analyses showed that urinary &bgr;ig-h3 was associated with a significant increase in the risk of microalbuminuria and proteinuria in diabetic patients.</P><P>Conclusions </P><P>Longitudinal monitoring of urinary &bgr;ig-h3 may improve the likelihood of detecting diabetic nephropathy at an earlier stage and &bgr;ig-h3 could be a sensitive marker of diabetic kidney disease progression.</P>

Expression and N-glycan analysis of human 90K glycoprotein in Drosophila S2 cells

Kim, K.R.,Kim, Y.K.,Cheong, H.,Kim, J.Y.H.,Cha, H.J. IPC Science and Technology Press ; Elsevier Scienc 2013 Enzyme and microbial technology Vol.53 No.3

Human 90K (h90K; Mac-2-binding protein) glycoprotein is a potential pharmaceutical due to its inhibitory activity against cancer metastasis and expansion. Here, h90K glycoprotein was produced in insect Drosophila S2 cell system, and its N-glycan pattern was analyzed. A plasmid encoding h90K gene, fused with a hexahistidine tag under the control of Drosophila metallotionein promoter, was stably transfected into S2 cells. After copper sulfate induction, transfected S2 cells secreted recombinant h90K at a good expression level of 28mg/L in a 150-mL spinner flask culture. The purified recombinant h90K showed an apparent molecular weight of ~78kDa which was much smaller than that (~97kDa) of the natural h90K. Because de-N-glycosylated h90K appeared at ~60kDa protein band, it was suggested that the recombinant h90K from S2 cells has small N-glycans with about half the molecular weight (~18kDa) of N-glycans of the natural h90K. Through detail analyses using high-performance liquid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, the S2-derived recombinant h90K was confirmed that it has simple paucimannosidic structures containing two or three mannose residues with core fucose as the major (~79%) N-glycans.

광소자 정렬용 초정밀 다축 스테이지 개발

정상화,이경형,김광호,차경래,김현욱,최석봉,박준호 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

As optical fiber communication grows, the fiber alignment become the focus of industrial attention. This greatly influence the overall production rates for the opto-electric products. We proposed multi-axis nano positioning stage for optical fiber alignment. This device has 3 DOF translation and sub nanometer resolution. This nano stage consist of 3 PZT-driven flexure stages which are stacked parallel. The displacement of it is measured with capacitance gauge and is controlled by computer-embedded main controller. The design process of flexure stage using FEM is proposed and the performance evaluation of this system is verified with experiments.

ADAMS를 이용한 리튬전지 고속 자동 블리스터 포장기의 가상시제품 개발

정상화,이경형,차경래,송석,최석환,신병수 朝鮮大學校 機械技術硏究所 2003 機械技術硏究 Vol.6 No.2

The blister packaging is applied t o many fields in recent years for i t s merit t h a t it enable consumer t o see t h e products The most of battery packaging a r e blister type. However, t h e lithium battery is dealt with very carefully i n packaging because of i t s explosion. The existing packaging machines for lithium battery are mostly adapted alkaline packaging machine and their capacity is very inferior to okher process. either. In this paper, the virtual prototype of the automatic blister packaging system for lithium battery which has new mechanism and 24OEAjmin capacity ' is developed and its performance is evaluated.

Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer

Jones, K. R.,Joo, Y. M.,Jang, S.,Yoo, Y.-J.,Lee, H. S.,Chung, I.-S.,Olsen, C. H.,Whitmire, J. M.,Merrell, D. S.,Cha, J.-H. American Society for Microbiology 2009 Journal of clinical microbiology Vol.47 No.4

<P>Helicobacter pylori causes diseases ranging from gastritis to peptic ulcer disease to gastric cancer. Geographically, areas with high incidences of H. pylori infection often overlap with areas with high incidences of gastric cancer, which remains one of the leading causes of cancer-related deaths worldwide. Strains of H. pylori that carry the virulence factor cytotoxin-associated gene A (cagA) are much more likely to be associated with the development of gastric cancer. Moreover, particular C-terminal polymorphisms in CagA vary by geography and have been suggested to influence disease development. We conducted a large-scale molecular epidemiologic analysis of South Korean strains and herein report a statistical link between the East Asian CagA EPIYA-ABD genotype and the development of gastric cancer. Characterization of a subset of the Korean isolates showed that all strains from cancer patients expressed and delivered phosphorylatable CagA to host cells, whereas the presence of the cagA gene did not strictly correlate to expression and delivery of CagA in all noncancer strains.</P>

The E3 ubiquitin ligase CHIP selectively regulates mutant epidermal growth factor receptor by ubiquitination and degradation

Chung, C.,Yoo, G.,Kim, T.,Lee, D.,Lee, C.S.,Cha, H.R.,Park, Y.H.,Moon, J.Y.,Jung, S.S.,Kim, J.O.,Lee, J.C.,Kim, S.Y.,Park, H.S.,Park, M.,Park, D.I.,Lim, D.S.,Jang, K.W.,Lee, J.E. Academic Press 2016 Biochemical and biophysical research communication Vol.479 No.2

Somatic mutation in the tyrosine kinase domain of epidermal growth factor receptor (EGFR) is a decisive factor for the therapeutic response to EGFR tyrosine kinase inhibitors (EGFR-TKIs) in lung adenocarcinoma. The stability of mutant EGFR is maintained by various regulators, including heat shock protein 90 (Hsp90). The C terminus of Hsc70-interacting protein (CHIP) is a Hsp70/Hsp90 co-chaperone and exhibits E3 ubiquitin ligase activity. The high-affinity Hsp90-CHIP complex recognizes and selectively regulates their client proteins. CHIP also works with its own E3 ligase activity independently of Hsp70/Hsp90. Here, we investigated the role of CHIP in regulating EGFR in lung adenocarcinoma and also evaluated the specificity of CHIP's effects on mutant EGFR. In HEK 293T cells transfected with either WT EGFR or EGFR mutants, the overexpression of CHIP selectively decreased the expression of certain EGFR mutants (G719S, L747_E749del A750P and L858R) but not WT EGFR. In a pull-down assay, CHIP selectively interacted with EGFR mutants and simultaneously induced their ubiquitination and proteasomal degradation. The expressions of mutant EGFR in PC9 and H1975 were diminished by CHIP, while the expression of WT EGFR in A549 was nearly not affected. In addition, CHIP overexpression inhibited cell proliferation and xenograft's tumor growth of EGFR mutant cell lines, but not WT EGFR cell lines. EGFR mutant specific ubiquitination by CHIP may provide a crucial regulating mechanism for EGFR in lung adenocarcinoma. Our results suggest that CHIP can be novel therapeutic target for overcoming the EGFR TKI resistance.

초등교사의 감정노동, 사회적 지지와 삶의 질 간의 관계

경예빈,김보민,김수정,노지현,송지윤,염소희,이현아,허유림,홍소연,차지영,박수민 이화여자대학교 간호과학대학 2017 이화간호학회지 Vol.- No.51

Purpose: The purpose of this study was to examine the correlation of emotional labor, social support, and quality of life among elementary school teacher. Methods: A correlational descriptive design was used with convenience sampling between July and December, 2016. The survey was self reported questionnaire administered to 130 elementary school teachers in the urban area. Data was statistically analyzed using t-test and one way ANOVA. Results: The average score of participants' emotional labor is 2.80, social support 3.89 and quality of life 3.65 of the total of 5. Emotional labor was negatively correlated to social support (r=-.178, p<.05) and quality of life(r=-.438, p<.001). Social support was positively correlated to quality of life (r=.336, p<.001). Conclusion: This study has significance as a basic data to understand the relation of emotional labor, social support, quality of life among elementary school teachers. Also, nursing interventions suitable for elementary school teachers should be developed to decrease the level of emotional labor, strengthen the social support, and enhance their quality of life.

중년남성의 지각된 건강상태, 가족응집력 및 건강증진행위에 관한 연구

김지선,노원영,박새별,유동민,이가은,이주애,전유라,조세영,차지영,고지현 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

health promotion behavior of middle aged men and the relationship among the above factors. Methods: Subjects of the research were 325 middle aged men in Korea. The questionnaires were composed of general characteristics, Health Self Rating Scale, FACES-Ⅲ : Family Adaptability & Cohesion Evaluation Scale Ⅲ, Health Promoting Lifestyle Profile. Data were statistically analyzed by t-test, ANOVA, Scheffe test and Pearson’s correlation. Results: Middle aged men whose perceived economic status are low show lower perceived health status than moderate and high (F=3.364, p=.010). Health promotion behavior in the age 40-44 shows lower level than 60-64 (F=2.984, p=.019). Middle aged men who have Economically dependent adult in the first child show higher level in health promotion behavior than who have Middle & High school student in the first child (F=2.468, p=.045). In the middle aged men, perceived health status and family cohesion show positive correlation (r=.341, p<.01). Perceived health status and health promotion behavior show positive correlation (r=.500, p<.01). Family cohesion and health promotion behavior show posotive correlation (r=.564, p<.01). Conclusions: We analyzed influencing factor and a relationship between perceived health status, family cohesion and health promotion behavior. Accordingly, we can think of applying nursing interventions to the middle aged men considering the general characteristics. Also we can think of applying nursing interventions and educations to help them evaluate their perceived health status and include the support of family members for the health promotion behavior.

유지혈액투석 환자에 있어 영양상태와 염증지표의 관계에 대한 연구

한상엽(S. Y. Han),조상경(S. K. Jo),윤종우(J. W. Yoon),신진호(J. H. Shin),이소영(S. Y. Lee),차대룡(D. R. Cha),권영주(Y. J. Kwon),조원용(W. Y. Cho),표희정(H. J. Pyo),정태시(T. S. Jung) 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.2

N/A Malnutrition is known to be an independent risk factor of morbidity and mortality in hemodialysis patients. The causes of malnutrition were obsucure. Several catabolic cytokines such as interleukin-1β(IL-1β), interleukin-6(IL-6), and tumar necrosis fac-tor-α(TNF-α) were reported to be elevated in hemo-dialysis patients with malnutrition, To evaluate the nutritrional status and relationship among cytokines IL-1β, TNF-α, and various parameters, we performed a cross-sectional study in maintenance hemodialysis patients who have undergone for six months at least. Patients who were taking steroid. showed any sign of infection, or had residual renal function were excluded from the study. Nutritional status was classified into well nourished(A), mild to moderate malnutrition(B), and severe malnutrition(C) by using subjective global assessment(SGA). Patients were divided into two groups (I: A, II: B and C) based on SGA. The level of IL-10, TNF-a were estimated by ELISA. Total patients were 23(M/F:15/8 mean age 52.0±1.56yrs). There were significant differences in weight changes for 6 months, CRP, and SGA between two groups based on the level of serum albumin(using 4.0g/dL as a cutoff value). Moreover, based on SGA, there were significant differences in age, BMI, CRP, and albumin between the group I and IL Serum albumin levels was directly correlated with transferrin(r=0.302, p=0.04), and was inversly correlated with age(r=-0.329, p=0.007). TNF- u was inversly correlated with Kt/Vueea(r=-0.291, p= 0.047), and directly correlated with the duration of maintenance hemodialysis(r=0,346, p=0.036), BMI(r-0.453, p<0.05). These data support the fact that albumin and CRP can be a good marker of nutritional status in maintenance hemodialysis patient. However cytokines and transferrin might not be related to nutritional status.

Studies of aqueous U(VI)-thiosalicylate complex formation via UV-Vis absorption spectrophotometry, TRLFS and potentiometry

Cha, W.,Cho, H.R.,Jung, E.C. Pergamon Press 2013 Polyhedron Vol.55 No.-

The formation of U(VI)-thiosalicylate (TSal) complexes in aqueous solutions (I=0.1M) was investigated over a wide range of pH values (from 3 to 9) using UV-Vis absorption and time-resolved luminescence spectroscopy, as well as by a potentiometric technique. It is demonstrated that the formation of U(VI)-TSal complexes, including monodentate, bidentate and ternary complexes, is responsible for the enhanced solubility of U(VI) and the strong U(VI)-luminescence quenching effects by TSal. In this study the stability constants of the complexes as well as two pK<SUB>a</SUB> values of TSal (pK<SUB>a1</SUB>=3.76+/-0.05; pK<SUB>a2</SUB>=8.33+/-0.07) are provided. The spectrophotometric analysis confirms the presence of both 1:1 and 1:2 (U(VI):TSal) charge-transfer complexes (logβ<SUB>101</SUB>=7.59+/-0.04 and logβ<SUB>102</SUB>=13.67+/-0.09). In luminescence quenching studies, mixed (dynamic and static) quenching processes are observed. The dynamic quenching for UO<SUB>2</SUB><SUP>2+</SUP> is attributed to bimolecular excited-state processes by both the acidic and monobasic forms of TSal, i.e., H<SUB>2</SUB>TSal and HTSal<SUP>-</SUP>. The static quenching processes are explained by the formation of non-radiative ground-state complexes: UO<SUB>2</SUB>(HTSal)<SUP>+</SUP> (logβ<SUB>111</SUB>=12.11+/-0.09) at pH 3.0; UO<SUB>2</SUB>(OH)(TSal)<SUP>-</SUP> and UO<SUB>2</SUB>(OH)<SUB>2</SUB>(TSal)<SUP>2-</SUP> (logβ<SUB>1-11</SUB>=3.9+/-0.1 and logβ<SUB>1-21</SUB>=-3.8+/-0.1, respectively) at a higher pH. In particular, the formation of the latter ternary species is found to be responsible for the strong static quenching of hydroxo-uranyl species, i.e. (UO<SUB>2</SUB>)<SUB>2</SUB>(OH)<SUB>2</SUB><SUP>2+</SUP> and (UO<SUB>2</SUB>)<SUB>3</SUB>(OH)<SUB>5</SUB><SUP>+</SUP>.