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      • SCIESCOPUS

        Simultaneous Detection of Tartrazine-Sunset Yellow in Food Samples Using Bioxide/Carbon Paste Microcomposite with Lanthanum and Titanium

        Nagles, Edgar,Ceroni, Mario,Hurtado, John The Korean Electrochemical Society 2020 Journal of electrochemical science and technology Vol.11 No.4

        This report describes the simultaneous detection of the two dyes most commonly used in food, tartrazine (TZ) and sunset yellow (SY), based on a microcomposite of carbon paste decorated with La<sub>2</sub>O<sub>3</sub> and TiO<sub>2</sub>. Anodic currents for SY-TZ were observed at 0.89-1.21 V by cyclic voltammetry (CV) separated with a ΔV of 0.32 V. The increased anodic peak currents compared to that of the unmodified carbon paste electrode were almost 50 and 41% for SY-TZ, respectively. The detection limits with the optimal amount of La<sub>2</sub>O<sub>3</sub>-TiO<sub>2</sub> were 0.02 and 0.03 µmol/L, respectively. The relative standard deviation (RSD) based on fifty measurements was less than 3%. The versatility and novelty of the sensor were tested with food samples containing these substances and other substances.

      • SCOPUSKCI등재

        Two-Layer Hand-Sewn Esophagojejunostomy in Totally Laparoscopic Total Gastrectomy for Gastric Cancer

        Norero, Enrique,Munoz, Rodrigo,Ceroni, Marco,Manzor, Manuel,Crovari, Fernando,Gabrielli, Mauricio The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.3

        Purpose: Different esophagojejunostomy (EJ) reconstruction methods are used after totally laparoscopic total gastrectomy (TLTG), and none is considered a standard technique. This report describes a 2-layer hand-sewn EJ technique during TLTG; we also evaluated postoperative morbidity associated with this technique. Materials and Methods: This retrospective cohort study included all consecutive patients who underwent TLTG for gastric cancer (GC) from 2012 to 2016 at 2 affiliated teaching hospitals. All participating surgeons performed standardized intracorporeal 2-layer hand-sewn EJ. Results: We included 51 patients who underwent TLTG for GC and standardized EJ anastomosis. Twenty-seven (53%) were male, and the median age was 60 (36-87) years. The average operative time was $337{\pm}71minutes$ and intraoperative bleeding was $160{\pm}107mL$. There were no open conversions related to EJ. Postoperative morbidity was observed in 9 (17.0%) patients. There was no postoperative mortality. EJ leakage was observed in 2 patients (3.8%) and 1 patient (1.9%) developed EJ stenosis. Patients with leakage were managed non-operatively and the patient with stenosis required endoscopic dilation. The median length of hospital stay was 8 (6-29) days. Conclusions: Two-layer hand-sewn EJ during TLTG for GC is a feasible and safe technique. This method avoids a laparotomy for reconstruction and the disadvantages associated with laparoscopic introduction of mechanical staplers for EJ, and provides an alternative for alimentary tract reconstruction after TLTG.

      • KCI등재

        Two-Layer Hand-Sewn Esophagojejunostomy in Totally Laparoscopic Total Gastrectomy for Gastric Cancer

        Enrique Norero,Rodrigo Muñoz,Marco Ceroni,Manuel Manzor,Fernando Crovari,Mauricio Gabrielli 대한위암학회 2017 Journal of gastric cancer Vol.17 No.3

        Purpose: Different esophagojejunostomy (EJ) reconstruction methods are used after totally laparoscopic total gastrectomy (TLTG), and none is considered a standard technique. This report describes a 2-layer hand-sewn EJ technique during TLTG; we also evaluated postoperative morbidity associated with this technique. Materials and Methods: This retrospective cohort study included all consecutive patients who underwent TLTG for gastric cancer (GC) from 2012 to 2016 at 2 affiliated teaching hospitals. All participating surgeons performed standardized intracorporeal 2-layer hand-sewn EJ. Results: We included 51 patients who underwent TLTG for GC and standardized EJ anastomosis. Twenty-seven (53%) were male, and the median age was 60 (36–87) years. The average operative time was 337±71 minutes and intraoperative bleeding was 160±107 mL. There were no open conversions related to EJ. Postoperative morbidity was observed in 9 (17.0%) patients. There was no postoperative mortality. EJ leakage was observed in 2 patients (3.8%) and 1 patient (1.9%) developed EJ stenosis. Patients with leakage were managed non-operatively and the patient with stenosis required endoscopic dilation. The median length of hospital stay was 8 (6–29) days. Conclusions: Two-layer hand-sewn EJ during TLTG for GC is a feasible and safe technique. This method avoids a laparotomy for reconstruction and the disadvantages associated with laparoscopic introduction of mechanical staplers for EJ, and provides an alternative for alimentary tract reconstruction after TLTG.

      • KCI등재후보

        Experimental behaviour of composite beams subjected to a hogging moment

        Marisa Pecce,Fernando Rossi,Fabio Antonio Bibbò,Francesca Ceroni 국제구조공학회 2012 Steel and Composite Structures, An International J Vol.12 No.5

        The present work addresses the rotational capacity of steel-concrete composite beams, which is a key issue for the seismic design of composite frames. Several experimental tests from the literature are summarised, and the effects of various parameters on the available plastic rotation are discussed. Furthermore, a number of remarks are made regarding the need for supplementary experimental results. The authors carried out experimental tests on four composite beams in which the type, width and connection degree of the slab were varied. During the tests, the deflection and strains in the steel profiles and bars were measured and recorded, wherein the observed trends in the measured parameters indicated that the failure mode of the beam was influenced by global and local buckling. A comparison of the experimental results to the theoretical ultimate strengths and moment-curvature relationships confirms that buckling phenomena occurred after section yielding, even if a consistent plastic rotation developed. This rotational capacity is well evaluated by a formulation that is available in the literature.

      • SCISCIESCOPUS

        A motor neuron strategy to save time and energy in neurodegeneration: adaptive protein stoichiometry

        Zucchi, Elisabetta,Lu, Ching‐,Hua,Cho, Yunju,Chang, Rakwoo,Adiutori, Rocco,Zubiri, Irene,Ceroni, Mauro,Cereda, Cristina,Pansarasa, Orietta,Greensmith, Linda,Malaspina, Andrea,Petzold, Axel Raven Press [etc.] 2018 Journal of neurochemistry Vol.146 No.5

        <P><B>Abstract</B></P><P>Neurofilament proteins (Nf) are a biomarker of disease progression in amyotrophic lateral sclerosis (ALS). This study investigated whether there are major differences in expression from <I>in vivo</I> measurements of neurofilament isoforms, from the light chain, NfL (68 kDa), compared with larger proteins, the medium chain (NfM, 150 kDa) and the heavy (NfH, 200‐210 kDa) chains in ALS patients and healthy controls. New immunological methods were combined with Nf subunit stoichiometry calculations and Monte Carlo simulations of a coarse‐grained Nf brush model. Based on a physiological Nf subunit stoichiometry of 7 : 3 : 2 (NfL:NfM:NfH), we found an ‘adaptive’ Nf subunit stoichiometry of 24 : 2.4 : 1.6 in ALS. Adaptive Nf stoichiometry preserved NfL gyration radius in the Nf brush model. The energy and time requirements for Nf translation were 56 ± 27k ATP (5.6 h) in control subjects compared to 123 ± 102k (12.3 h) in ALS with ‘adaptive’ (24:2.4:1.6) Nf stoichiometry (not significant) and increased significantly to 355 ± 330k (35.5 h) with ‘luxury’ (7:3:2) Nf subunit stoichiometry (<I>p</I> < 0.0001 for each comparison). Longitudinal disease progression‐related energy consumption was highest with a ‘luxury’ (7:3:2) Nf stoichiometry. Therefore, an energy and time‐saving option for motor neurons is to shift protein expression from larger to smaller (cheaper) subunits, at little or no costs on a protein structural level, to compensate for increased energy demands.</P><P/>

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