Couple-group L2-L∞ Consensus of Nonlinear Multi-agent Systems with Markovian Switching Topologies

Xiao Li,Cancan Zhou,Jianping Zhou,Zhen Wang,Jianwei Xia 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.3

The paper is devoted to the couple-group L2-L∞ consensus problem of nonlinear multi-agent systemsaffected by external disturbances. The interaction topologies among agents obey a continuous-time Markovianprocess with unknown transition probabilities. By a system transformation, the problem of couple-group L2-L∞ consensus is converted into a L2-L∞ control issue. Then, by Lyapunov stability theory and graph theory, sufficientconditions for the couple-group L2-L∞ consensus are obtained. The control gains can be acquired via the solutionsof a group of linear matrix inequalities. Moreover, the present method is extended to the multi-group L2-L∞consensus. Finally, an example is provided to illustrate the effectiveness of the results.

Fuzzy comprehensive performance evaluation method of rolling linear guide based on improved analytic hierarchy process

Yali Ma,Jiayong Wei,Cancan Li,Chen Liang,Guochao Liu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.7

Aiming at the problem that the multi-objective evaluation of performance and the weighting coefficients of indexes is difficult to find, a fuzzy comprehensive performance evaluation method of rolling linear guide (RLG) is proposed. Considering the static and dynamic indexes, a structure model containing nine weighting coefficients was constructed. The performance evaluation function was established by the compromise programming method and the average power method, while weighting coefficients were determined by the improved analytic hierarchy process (I-AHP); the mathematical model was obtained to evaluate the RLG in a certain application. The evaluation results show that the confidence of the static, dynamic and comprehensive performance of the RLG is 59.21 %, 81.97 %, and 63.34 %, respectively. The feasibility of the method was verified by numerical simulation. This method indicates the pros and cons of the performance of the RLG and also indicates the direction for its optimized design.

A novel immune-related LncRNA prognostic signature for cutaneous melanoma

Hu Nan,Huang Cancan,He Yancheng,Li Shuyang,Yuan Jingyi,Zhong Guishu,Chen Yan 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Backgrounds Among tumor microenvironment, the immune components in it have an important influence on gene expression and clinical efficacy. We aim to find out the role of those in skin cutaneous melanoma (SKCM). Objectives Gene expression profile and homologous clinical information of SKCM patients were obtained by TCGA (The Cancer Genome Atlas) and UCSC Toil. SsGSEA method was used to evaluate the immune cell infiltration of 468 TCGA-SKCM samples divided into high immune cell infiltration group (HICI) and low immune cell infiltration group (LICI). We used the Edger packet to conduct difference analysis on normal samples (GTEx) and cancer samples (TCGA), and combined it with the difference of the HICI group and LICI group, to find out the common differential expression of lncRNA in both groups. The prognostic value of immune-related lncRNAs was studied by univariate Cox, Lasso-Cox and multivariate Cox regression analysis, and a prognostic model was established. C index and calibration diagram were used to judge the accuracy of the model, and DCA was used to judge the net benefit. Results Six prognostic markers of immune-related lncRNA genes were established, which could be used as independent prognostic factors. The net benefit and prediction accuracy are significantly higher than TNM Stage. Conclusion The prognostic model identified in this study is a reliable biomarker for SKCM. The Nomogram survival prediction model based on it is a reliable way to predict the median survival time of patients, which may lay the foundation for future treatment of this disease. Backgrounds Among tumor microenvironment, the immune components in it have an important influence on gene expression and clinical efficacy. We aim to find out the role of those in skin cutaneous melanoma (SKCM). Objectives Gene expression profile and homologous clinical information of SKCM patients were obtained by TCGA (The Cancer Genome Atlas) and UCSC Toil. SsGSEA method was used to evaluate the immune cell infiltration of 468 TCGA-SKCM samples divided into high immune cell infiltration group (HICI) and low immune cell infiltration group (LICI). We used the Edger packet to conduct difference analysis on normal samples (GTEx) and cancer samples (TCGA), and combined it with the difference of the HICI group and LICI group, to find out the common differential expression of lncRNA in both groups. The prognostic value of immune-related lncRNAs was studied by univariate Cox, Lasso-Cox and multivariate Cox regression analysis, and a prognostic model was established. C index and calibration diagram were used to judge the accuracy of the model, and DCA was used to judge the net benefit. Results Six prognostic markers of immune-related lncRNA genes were established, which could be used as independent prognostic factors. The net benefit and prediction accuracy are significantly higher than TNM Stage. Conclusion The prognostic model identified in this study is a reliable biomarker for SKCM. The Nomogram survival prediction model based on it is a reliable way to predict the median survival time of patients, which may lay the foundation for future treatment of this disease.

Identification of a novel cytoplasmic male sterile line M2BS induced by partial-length HcPDIL5-2a transformation in rice (Oryza sativa L.)

Danfeng Tang,Peng Chen,Gang Jin,Min Li,Cancan Wang,Zhenlan Bin,Jinghua Qian,Zizheng Wang,Ruiyang Zhou 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2

A cytoplasmic male sterile line (designated as M2BS) was obtained from an indica rice maintainer M2B induced by partial-length HcPDIL5-2a (Hibiscus cannabinus protein disulfide isomerase-like) transformation. The anther of M2BS was short, slender, hygrophanous, and fissured. I2-KI staining method showed that there was typical and spherical abortion in pollen grains. M2BS was found abortive at middle and late stage of monocyte by the modified carbol fuchsin stained observation and paraffin section observation. The tapetum was observed pre-degenerated in M2BS. Hereditary analysis indicated that the male sterility of M2BS was a maternally inherited inability after six backcross generations with M2B and the combinations of M2BS hybridized with other two male fertile materials. The M2BS could be affirmed a cytoplasmic male sterile (CMS) type. Moreover, it was a transgenic plant confirmed by PCR, Southern blot and RT-PCR detection. M2BS could be distinguished from M2B and its CMS line M2A by RFLP analysis. The overall mitochondrial genome sequencing results showed, that in M2BS, the main differences of mitochondrial gene sequence were located in nad4, nad5, nad7, orf194 and intergenic region, relatively to those of M2A. The obtained results indicate that M2BS is a novel cytoplasmic male sterile line.

Illuminating the hepatotoxic mechanism of norcantharidin in rats using metabolomics analysis

Cheng Weina,Chen Qihong,Wang Xiaoning,Liu Liu,Li Xiaofei,Duan Cancan,Zhang Jianyong 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3

Norcantharidin (NCTD) has multiple antitumor effects. However, NCTD can induce significant hepatotoxicity and the mechanism of hepatotoxicity is not clear for now.This study aimed to explore the hepatotoxicity of NCTD in rat by ultra-performance liquid chromatography (UPLC) quadrupole time-of-flight (Q-TOF)-MS (UPLC/Q-TOF-MS) metabolomics.Serum biochemical indices including alanine aminotransferase (ALT) and total bilirubin (T-BIL) were significantly increased. Histopathological and ultrastructure results revealed that hepatocytes were damaged. Furthermore, the metabolomics results showed that 11 metabolites in serum and 8 metabolites in liver were differential metabolites for NCTD hepatotoxicity. Four metabolic pathways including the sphingolipid metabolism, purine metabolism, arachidonic acid metabolism, and glycerophospholipid metabolism were the key metabolic pathways related to NCTD hepatotoxicity.The metabolomics analysis in this study reveal new clues on the hepatotoxicity mechanism of NCTD in rats. These findings have potential applications in the toxicity study of NCTD.

Genome-wide association analysis of nine reproduction and morphological traits in three goat breeds from Southern China

Sun Xiaoyan,Jiang Jing,Wang Gaofu,Zhou Peng,Li Jie,Chen Cancan,Liu Liangjia,Li Nianfu,Xia Yuanyou,Ren Hangxing 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.2

Objective: This study aimed to investigate the significant single nucleotide polymorphisms (SNPs) and genes associated with nine reproduction and morphological traits in three breed populations of Chinese goats. Methods: The genome-wide association of nine reproduction and morphological traits (litter size, nipple number, wattle, skin color, coat color, black dorsal line, beard, beard length, and hind leg hair) were analyzed in three Chinese native goat breeds (n = 336) using an Illumina Goat SNP50 Beadchip. Results: A total of 17 genome-wide or chromosome-wide significant SNPs associated with one reproduction trait (litter size) and six morphological traits (wattle, coat color, black dorsal line, beard, beard length, and hind leg hair) were identified in three Chinese native goat breeds, and the candidate genes were annotated. The significant SNPs and corresponding putative candidate genes for each trait are as follows: two SNPs located on chromosomes 6 (CSN3) and 24 (TCF4) for litter size trait; two SNPs located on chromosome 9 (KATNA1) and 1 (UBASH3A) for wattle trait; three SNPs located on chromosome 26 (SORCS3), 24 (DYM), and 20 (PDE4D) for coat color trait; two SNPs located on chromosome 18 (TCF25) and 15 (CLMP) for black dorsal line trait; four SNPs located on chromosome 8, 2 (PAX3), 5 (PIK3C2G), and 28 (PLA2G12B and OIT3) for beard trait; one SNP located on chromosome 18 (KCNG4) for beard length trait; three SNPs located on chromosome 17 (GLRB and GRIA2), 28 (PGBD5), and 4 for hind leg hair trait. In contrast, there were no SNPs identified for nipple number and skin color. Conclusion: The significant SNPs or genes identified in this study provided novel insights into the genetic mechanism underlying important reproduction and morphological traits of three local goat breeds in Southern China as well as further potential applications for breeding goats.

Activating SIRT3 in peritoneal mesothelial cells alleviates postsurgical peritoneal adhesion formation by decreasing oxidative stress and inhibiting the NLRP3 inflammasome

Shen Tianli,Wu Yunhua,Wang Xingjie,Wang Zijun,Li Enmeng,Zhou Cancan,Yue Chenyang,Jiang Zhengdong,Wei Guangbing,Lian Jie,Xu Qinhong,Li Xuqi 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Peritoneal adhesions (PAs) are a serious complication of abdominal surgery and negatively affect the quality of life of millions of people worldwide. However, a clear molecular mechanism and a standard therapeutic strategy for PAs have not been established. Here, we developed a standardized method to mimic the pathological changes in PAs and found that sirtuin 3 (SIRT3) expression was severely decreased in adhesion tissues, which was consistent with our bioinformatics analysis and patient adhesion tissue analysis. Thus, we hypothesized that activating SIRT3 could alleviate postsurgical PAs. Sirt3-deficient (Sirt3−/−) mice exhibited many more PAs after standardized abdominal surgery. Furthermore, compared with wild-type (Sirt3+/+) mice, Sirt3-deficient (Sirt3−/−) mice showed more prominent reactive oxygen species (ROS) accumulation, increased levels of inflammatory factors, and exacerbated mitochondrial damage and fragmentation. In addition, we observed NLRP3 inflammasome activation in the adhesion tissues of Sirt3−/− but, not Sirt3+/+ mice. Furthermore, mesothelial cells sorted from Sirt3−/− mice exhibited impaired mitochondrial bioenergetics and redox homeostasis. Honokiol (HKL), a natural compound found in several species of the genus Magnolia, could activate SIRT3 in vitro. Then, we demonstrated that treatment with HKL could reduce oxidative stress and the levels of inflammatory factors and suppress NLRP3 activation in vivo, reducing the occurrence of postsurgical PAs. In vitro treatment with HKL also restored mitochondrial bioenergetics and promoted mesothelial cell viability under oxidative stress conditions. Taken together, our findings show that the rescue of SIRT3 by HKL may be a new therapeutic strategy to alleviate and block postsurgical PA formation.