Korean Byungkyul - Citrus platymamma Hort.et Tanaka flavonoids induces cell cycle arrest and apoptosis, regulating MMP protein expression in Hep3B hepatocellular carcinoma cells

Hong, G. E.,Lee, H. J.,Kim, J. A.,Yumnam, S.,Raha, S.,Saralamma, V. Venkatarame Gowda,Heo, J. D.,Lee, S. J.,Kim, E. H.,Won, C. K. Lychnia 2017 International journal of oncology Vol.50 No.2

<P>Citrus platymamma Hort.et Tanaka is an indigenous fruit of Jeju island in Korea. In this study the bioactivity of C. platymamma flavonoids were evaluated on human hepatoma Hep3B cell lines. Eleven flavonoids were identified from the peels of C. platymamma Hort.et Tanaka through high-performance liquid chromatography-Tandem mass spectrometry and the anticancer effect of these C. platymamma flavonoids on human hepatoma Hep3B were studied. Chromatin condensation was observed in Hep3B cells treated with C. platymamma flavonoids. DNA fragmentation was confirmed through agarose gel electrophoresis and TUNEL assay. An increase in the total apoptotic cells and G2/M cell cycle arrest with decreased protein expression of CDC25C, CDK1, cycl in B1 and p21 were observed in Hep3B cells treated with flavonoids of C. platymamma. Further, protein expression of Bcl-XL, Bax, caspase-3 and -9 were also modulated by C. platymamma flavonoids treatment indicating that cell death is through intrinsic apoptotic pathway. Moreover, C. platymamma flavonoids also regulated the phosphorylation of MAPKs, PI3K, and Akt in Hep3B cells. Relevant to inhibiting metastasis, C. platymamma treatment reduced wound closure of Hep3B cells and the protein expression of matrix metalloproteinase-2 and -9 were reduced in C. platymamma treated cells. The results show that C. platymamma flavonoids induce cell cycle arrest and apoptosis following activation of MAPKs and suppression of PI3K/Akt pathway which eventually inhibits cell migration in Hep3B cells. The finding provides evidence on biochemical activities of C. platymamma Hort.et Tanaka, which would be an essential agent for hepatocellular carcinoma (HCC) treatment.</P>

TMS320C31을 이용한 얼굴 검출용 하드웨어 구현

박일용,진경찬,채승표,원철호,손병기,신장규,조진호 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

The real-time pattern recognition is widely used in the image recognition or auto sentry systems to be implemented by the DSP chip which is adequate for real-time processing. The pattern recognition can be performed by two methods, i.e. the feature-based matching and template one mainly. Generally, the feature-based matching, such as DLM(dynamic linking matching), is very difficult to utilize as a software for the development of real-time processing system. So as to be possible for real-time processing is the template matching desirable. In this paper, we have proposed the image acquisition and processing system for face detection. By applying the PCA(principal component analysis), we made the template to be matched with the input image acquired by the implemented system. And then the face region could be extracted from the input image by the computations of the Euclidean distances between the template and the sub-images. The system, which is based on the TMS320C31 DSP chip, consists of the video signal A/D, D/A converter, image acquisition part, and DSP part.

Novel anti-apoptotic mechanism of A20 through targeting ASK1 to suppress TNF-induced JNK activation

Won, M,Park, K A,Byun, H S,Sohn, K-C,Kim, Y-R,Jeon, J,Hong, J H,Park, J,Seok, J H,Kim, J M,Yoon, W-H,Jang, I-S,Shen, H M,Liu, Z G,Hur, G M Macmillan Publishers Limited 2010 CELL DEATH AND DIFFERENTIATION Vol.17 No.12

The zinc-finger protein A20 has crucial physiological functions as a dual inhibitor of nuclear factor-κB (NF-κB) activation and apoptosis in tumor necrosis factor (TNF) receptor 1 signaling pathway. Although the molecular basis for the anti-NF-κB function of A20 has been well elucidated, the anti-apoptotic function of A20 is largely unknown. Here, we report a novel mechanism underlying the anti-apoptotic function of A20: A20 blocks TNF-induced apoptosis through suppression of c-jun N-terminal kinase (JNK) by targeting apoptosis signal-regulating kinase1 (ASK1). First, the ectopic expression of A20 drastically inhibits TNF-induced JNK activation and apoptosis in multiple cell types including those deficient of NF-κB activation. Unexpectedly, the blunting effect of A20 on TNF-induced JNK activation is not mediated by affecting the TNFR1 signaling complex formation. Instead, A20 interacts with ASK1, an important MAPKK kinase in the JNK signaling cascade. More importantly, overexpression of wild-type A20, but not of mutant A20 (ZnF4; C624A, C627A), promotes degradation of the ASK1 through the ubiquitin-proteasome system. Taken together, the results from this study reveal a novel anti-apoptotic mechanism of A20 in TNF signaling pathway: A20 binds to ASK1 and mediates ASK1 degradation, leading to suppression of JNK activation and eventually blockage of apoptosis.

Data Build-up for the Construction of Korean Specific Greenhouse Gas Emission Inventory in Livestock Categories

Won, S.G.,Cho, W.S.,Lee, J.E.,Park, K.H.,Ra, C.S. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.3

Many studies on methane ($CH_4$) and nitrous oxide ($N_2O$) emissions from livestock industries have revealed that livestock production directly contributes to greenhouse gas (GHG) emissions through enteric fermentation and manure management, which causes negative impacts on animal environment sustainability. In the present study, three essential values for GHG emission were measured; i.e., i) maximum $CH_4$ producing capacity at mesophilic temperature ($37^{\circ}C$) from anaerobically stored manure in livestock category ($B_{0,KM}$, Korean livestock manure for $B_0$), ii) $EF_{3(s)}$ value representing an emission factor for direct $N_2O$ emissions from manure management system S in the country, kg $N_2O-N$ kg $N^{-1}$, at mesophilic ($37^{\circ}C$) and thermophilic ($55^{\circ}C$) temperatures, and iii) $N_{ex(T)}$ emissions showing annual N excretion for livestock category T, kg N $animal^{-1}$ $yr^{-1}$, from different livestock manure. Static incubation with and without aeration was performed to obtain the $N_2O$ and $CH_4$ emissions from each sample, respectively. Chemical compositions of pre- and post- incubated manure were analyzed. Contents of total solids (% TS) and volatile solid (% VS), and the ratio of carbon to nitrogen (C/N) decrease significantly in all the samples by C-containing biogas generation, whereas moisture content (%) and pH increased after incubation. A big difference of total nitrogen content was not observed in pre- and post-incubation during $CH_4$ and $N_2O$ emissions. $CH_4$ emissions (g $CH_4$ kg VS-1) from all the three manures (sows, layers and Korean cattle) were different and high C/N ratio resulted in high $CH_4$ emission. Similarly, $N_2O$ emission was found to be affected by % VS, pH, and temperature. The $B_{0,KM}$ values for sows, layers, and Korean cattle obtained at $37^{\circ}C$ are 0.0579, 0.0006, and 0.0828 $m^3$ $CH_4$ kg $VS^{-1}$, respectively, which are much less than the default values in IPCC guideline (GL) except the value from Korean cattle. For sows and Korean cattle, $N_{ex(T)}$ values of 7.67 and 28.19 kg N $yr^{-1}$, respectively, are 2.5 fold less than those values in IPCC GL as well. However, $N_{ex(T)}$ value of layers 0.63 kg N $yr^{-1}$ is very similar to the default value of 0.6 kg N $yr^{-1}$ in IPCC GLs for National greenhouse gas inventories for countries such as South Korea/Asia. The $EF_{3(s)}$ value obtained at $37^{\circ}C$ and $55^{\circ}C$ were found to be far less than the default value.

Sol-Gel 법에 의한 Al2O3 제조시 침전제 (H2C2O4) 의 영향

오재현,원창환,천병선,이정원 대한금속재료학회(대한금속학회) 1991 대한금속·재료학회지 Vol.29 No.1

A new way of producing alumina from the alkoxide is reported. Aluminum oxalate is first synthesized by reactions which allow controlled precipitation. Alumina powder is obtained by subsequent thermal decomposition. 1) The addition of H₂C₂O₄, in the Boehmite Sol. was able to reduce the Geling time. 2) The first generated particles in the size of 10㎛ were agglomerated in chain type. 3) The recovery of A1₂O₃ was increased with increasing PH of solution and decreasing both the mole ratio of H₂C₂O₄/ Alkoxide and reaction temperature. 4) The grain size of the calcined products was increased with increasing PH of solution, mole ratio of H₂C₂O₄/ Alkoxide and reaction temperature, however, decreased significantly in the range of PH 1.

SRM 구동을 위한 Energy Efficient C-Dump 컨버터에 관한 연구

최준혁(J.H. Choi),윤용호(Y.H. Yoon),송병섭(B.S. Song),원충연(C.Y. Won),김규식(G.S. Kim),최세완(S.W. Choi) 전력전자학회 2001 전력전자학술대회 논문집 Vol.2001 No.-

This paper compared a Modified C-dump converter and energy efficient converter topologies, derived from the conventional C-dump converter, for switched reluctance motor (SRM) drives The proposed topologies overcome the limitations of the conventional C-dump converter, and could reduce the whole cost of the SRM drive. Also, the above converters have simple control requirements, and allow the motor phase current to free wheel during chopping mode. Specially, the voltage ratings of the dump capacitor and some of the switching devices in the proposed an Energy efficient C-dump converter is reduced to the supply voltage (V-dc) level compared to twice the supply voltage (2V-dc) in the conventional C-dump converter. Simulation and experimental results of the proposed converters are presented and verified.<br/> <br/>

放射線從事者의 個人被曝線量測定 (Ⅳ)

李海龍,庾在昌,朴基正,吳賢珠,金鍾邵,金康淑,金昌均,元奉必 국립보건연구원 1989 국립보건원보 Vol.26 No.

Electrical properties of a Cu-germanide Schottky contact to n-type Ge depending on its microstructural evolution driven by rapid thermal annealing

Janardhanam, V.,Jyothi, I.,Lee, J.H.,Yun, H.J.,Won, J.,Lee, Y.B.,Lee, S.N.,Choi, C.J. Elsevier Sequoia 2017 THIN SOLID FILMS - Vol.632 No.-

The electrical properties of Cu-germanide(Cu<SUB>3</SUB>Ge)/n-type Ge Schottky contacts formed as a result of a solid state reaction between Cu and n-type Ge were investigated as a function of the rapid thermal annealing (RTA) temperature and correlated with its microstructural evolution driven by the RTA process. The variations of the barrier height of Cu<SUB>3</SUB>Ge/n-type Ge Schottky rectifiers caused by the RTA process were determined using current-voltage (I-V) and capacitance-voltage (C-V) methods. The Cu<SUB>3</SUB>Ge film formed after annealing at 400<SUP>o</SUP>C exhibited a relatively uniform surface and interface morphology. This led to the formation of a laterally homogenous Schottky barrier in the Cu<SUB>3</SUB>Ge/n-type Ge Schottky diode, resulting in an improvement of its rectifying I-V behavior. On the other hand, after annealing above 500<SUP>o</SUP>C, the Cu<SUB>3</SUB>Ge film was severely agglomerated without film continuity and eventually evolved into isolated islands at 600<SUP>o</SUP>C. Such structural degradation of Cu<SUB>3</SUB>Ge led to a rapid decrease in the barrier height and an increase in the reverse leakage current of the Cu<SUB>3</SUB>Ge/n-type Ge Schottky diode. The electric field dependence of the reverse current showed that the reverse leakage current in the Cu<SUB>3</SUB>Ge/n-type Ge Schottky diodes was dominated by a Poole-Frenkel emission mechanism, regardless of the RTA temperatures.

Fabrication of superconducting MgB₂ thin films on textured Cu(100) tape by hybrid physical–chemical vapor deposition

Lee, Tae Gyoung,Ranot, Mahipal,Seong, Won Kyung,Jung, Soon-Gil,Kang, W N,Joo, J H,Kim, C-J,Jun, B-H,Kim, Youngha,Zhao, Y,Dou, S X IOP Publishing Ltd 2009 Superconductor science & technology Vol.22 No.4

<P>We report on the fabrication of superconducting MgB<SUB>2</SUB> thin films on textured Cu(100) tape under low pressure and temperature by using a hybrid physical–chemical vapor deposition (HPCVD) technique to explore the possible broad range of deposition. Thermodynamic calculations for the Mg–B system have been carried out and the pressure–temperature phase diagram was obtained. Our results demonstrate that the deposition of superconducting MgB<SUB>2</SUB> films is possible beyond the theoretically calculated growth window, where the sublimation of Mg is taking place. The structural and microstructural investigations reveal that MgB<SUB>2</SUB> films are <I>c</I>-axis-oriented normal to the substrate. The highest <I>J</I><SUB>c</SUB> of ∼1.34 × 10<SUP>5</SUP> A cm<SUP>−2</SUP> at 5 K under 3 T is obtained for the film grown at 460 °C. The critical current density (<I>J</I><SUB>c</SUB>) and flux pinning force density (<I>F</I><SUB>p</SUB>) of MgB<SUB>2</SUB> films are enhanced with decreasing growth temperature. This could be attributed to the high density of grain boundaries which may act as effective flux pinning centers. These findings suggest an alternative route to fabricate MgB<SUB>2</SUB> tapes at low temperature for large scale applications.</P>

농후사료 급여수준이 비거세 한우의 증체와 부위별 지방조직의 지방산 조성에 미치는 효과

송만강,김내수,정정수,최양일,원유석,정재경,최성호 ( M . K . Song,N . S . Kim,C . S . Chung,Y . I . Choi,Y . S . Won,J . K . Chung,S . H . Choi ) 한국축산학회 1998 한국축산학회지 Vol.40 No.5

The study was conducted with 63 Hanwoo bull calves of 193 days old for 19 months(up to 26 month of age) to examine the effect of feeding level of concentrate on the body weight gain, feed requirements and fatty acid composition of adipose tissues of various locations(subcutaneous, abdominal, kidney, intermuscular and intramuscular fats). The calves were allotted into 3 treatments according to the feeding level of concentrate(85, 100 and 115%), and fed concentrates of Grower(7∼10 month of age), Finisher I(11∼16 month of age), Finisher II (17∼20 month of age) and Finisher III(21∼26 month of age). The feeding levels of concentrates for the cattle of 85% and 115% were decreased or increased by 15%, respectively, up to 15 month of age based on the similar body weight to the cattle of 100%. The feeding levels of the concentrates for the cattle of 85% and 100% were continuously increased for the next 2 months, to make same levels as for the cattle of I15%, thereafter amount of concentrate were continuously increased based on the rate of body gain for all the cattle. The cattle were fed in a individual feeding system through the experimental period. The cattle were slaughtered at the ages of 6, 14, 18 and 24 month and adipose tissues were collected to analyze long chain fatty acids. Results observed from the study were summarized as follows Overall mean dry matter intake and body gain per day and feed requirements of Hanwoo bulls during the 19 months of feeding from 193 day old calves were 6.69㎏, 0.95㎏ and 7.06, respectively. Body gain of the Hanwoo bulls during experimental period was greatly affected by the feeding level of concentrate, but feed requirement was improved up to 20 month of age with the decreased feeding level of concentrate. Fatty acids of C_(16:0) C_(18:0) and C_(18:1) in the adipose tissues at various locations of Hanwoo bulls were dominated. The feeding level of concentrate did not affect the fatty acid composition except for C_(16:1) and C_(18:1) in subcutaneous fat, and C_(18:1) and C_(18:2) in intramuscular fat of the cattle that were fed at the level of 85% at the age of 14 month(P$lt;.05). No differences were observed in fatty acid composition of adipose tissues of each location for the cattle of 18 and 24 month of age among treatments. There were tendencies of greater C_(16:1) and C_(18:1) in subcutaneous and intramuscular fats while higher C_(18:0) in abdominal fat. There were also trends of greater unsaturated fatty acid compositions in subcutaneous and intramuscular fats and higher saturated fatty acids in abdominal, kidney and intermuscular fats. Percent C_(18:0) tended to be decreased while C_(16:1) C_(18:1) and C_(18:2) increased with the age of Hanwoo bulls.