Cytological, histochemical, and ultrastructural study of human foetal liver of various gestation with future implications in segmental resection: an anatomical perspective

Arpan Haldar,Manisha Rajanand Gaikwad,Apurba Patra,Soumya C. Bhattacharya 대한해부학회 2022 Anatomy & Cell Biology Vol.55 No.1

The liver is the largest gland of the gastrointestinal tract having both exocrine and endocrine functions. Developmentally it arises as a ventral outgrowth from the gut endoderm during 3rd week of intrauterine life. The foetal liver is very important because of its synthetic and hemopoietic potential. The present work aimed to see the detailed histogenesis and development of the foetal liver by cytological, immunohistochemical and ultrastructural study. The liver tissue of nine aborted foetuses of various gestational age were studied. For cytology: special stains like Masson trichrome, periodic acid Schiff and reticulin were used, immunohistochemical staining was performed with triple antibodies (c-myc, Ki-67 and Ber-H2), and for ultrastructure: aluminium mounted specimens were coated with gold and argon gas and observed under scanning electron microscopy (EM). Cytology and immunohistochemistry showed the development of duct patterns and hemopoietic patterns in all stages of fetogenesis. The ductal plate was marked by the layer of dark brown staining cells at the edge of two portal tracts. The haemopoietic cells with sinusoids and aggregation of hepatocytes were observed in the early weeks of gestation. EM showed tree-like branching of a portal canal depicting hepatic segmentation of foetal liver. The organizational changes in lobular pattern, duct pattern, and microstructure of liver during fetogenesis are very crucial to achieve the adult morphology in feature. Histogenesis of the foetal liver follows a multistep process depending upon the gestational age, any deviation from normalcy may lead to structural and functional abnormality later in life.

Impacts of Photoperiod and Maternal Pineal Gland on Pre- and Post-natal development of Indian palm Squirrel F. pennanti

Haldar, C.,Bishnupuri, K.S. Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

Studies till date suggest the existence of a fetal biological clock in suprachiasmatic nuclei entrained by the circadian signal from mother. Melatonin from maternal pineal gland reaches to the fetus by crossing every biological barrier including placenta, hence fetuses were exposed to similar melatonin variation as their mother. Experimental modulations of maternal pineal gland activity of pregnant females either by exposing the them to different photoperiodic schedules or by exogenous melatonin treatments till the date of parturition, regulated the fetal plasma level of melatonin, thereby the prenatal (fetal) growth and development. This clearly suggests the maternal transport of melatonin to their fetus through placenta since fetal retina-hypothalamic tract was incomplete. An extension of experimental schedules till 60 days of post-partum period regulated the neonatal pineal gland activity and gonadal maturation along with their plasma levels of melatonin and sex steroids suggesting clearly the phenomenon of maternal transfer of melatonin to their young ones during the post-natal period, when the neonates were solely dependent on the mother's milk for their nutrition and energetic demands. On the basis of above observations we may suggest that the maternal pineal gland activity regulate the prenatal development by passing its melatonin to fetus via placenta and post-natal growth and sexual maturation by passing maternal melatonin to neonates via milk. Hence, the photoperiod perceived by mother is translated into the maternal plasma level of melatonin which not only regulates the prenatal but also the post-natal growth and sexual maturation of neonates.

APPLICATION OF DOUBLE DECOMPOSITION TO PULSATILE FLOW

C.Mamaloukas,K.Haldar,H.P.Mazumdar 한국전산응용수학회 2002 Journal of applied mathematics & informatics Vol.10 No.1

A high precision shear flexible element for bending analysis of thick/thin triangular plate

Haldar, S.,Das, P.,Manna, M.C. Techno-Press 2004 Structural Engineering and Mechanics, An Int'l Jou Vol.18 No.1

A high precision shear deformable triangular element has been proposed for bending analysis of triangular plate. The element has twelve nodes at the three sides and four nodes inside the element. Initially the element has thirty-five degrees of freedom, which has been reduced to thirty by eliminating the degrees of freedom of the internal nodes through static condensation. Plates having different boundary conditions, side ratios (b/a) and thickness ratios (h/a = 0.001, 0.1 and 0.2) have been analyzed using the proposed shear locking free element. Concentrated and uniformly distributed transverse loads have been used for the analysis. The formulation is made based on first order shear deformation theory. For validation of the present element and formulation few results of thin triangular plate have been compared with the analytical solutions. Results for thick plate have been presented as new results.

APPLICATION OF DOUBLE DECOMPOSITION TO PULSATILE FLOW

Mamaloukas, C.,Haldar, K.,Mazumdar, H.P. 한국전산응용수학회 2002 Journal of applied mathematics & informatics Vol.10 No.1

The present investigation deals with the pulsatile flow of incompressible viscous fluid through a circular rigid tube provided with constriction. The method applied here is the Decomposition Method, which has been developed by George Adomian [3]. The advantages of this method are the avoidance of simplifications and restrictions, which change the non-linear problem to mathematically tractable one, whose solution is not consistent with physical solution. Theoretically results, such as, wall shear stress and axial velocity component, have been obtained and the graphical solutions of these theoretical results have been shown in the figures.

A high precision shear deformable element for free vibration of thick/thin composite trapezoidal plates

S. Haldar,M. C. Manna 국제구조공학회 2003 Steel and Composite Structures, An International J Vol.3 No.3

A high precision shear deformable triangular element has been proposed for free vibration analysis of composite trapezoidal plates. The element has twelve nodes at the three sides and four nodes inside the element. Initially the element has fifty-five degrees of freedom, which has been reduced to forty-eight by eliminating the degrees of freedom of the internal nodes through static condensation. Plates having different side ratios (b/a), boundary conditions, thickness ratios (h/a=0.01, 0.1 and 0.2), number of layers and fibre angle orientations have been analyzed by the proposed shear locking free element. Trapezoidal laminate with concentrated mass at the centre has also been analyzed. An efficient mass lumping scheme has been recommended, where the effect of rotary inertia has been included. For validation of the present element and formulation few results of isotropic trapezoidal plate and square composite laminate have been compared with those obtained from open literatures. The numerical results for composite trapezoidal laminate have been given as new results.

Maternal photic regulation of immune status in neonates of Indian palm squirrel Funambulus pennanti

Bishnupuri, K.S.,Haldar, C.,Singh, R. Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

Till date the phenomenon of maternal transfer of photic information was reported to regulate the fetal/neonatal growth, however its influence on neonatal immune system is still an enigma. In the present study, we observed an increase in maternal plasma melatonin level under short day length (SOL) condition with a consequent decrease in TLC and LC in their respective neonates. However, a significant decrease in maternal plasma melatonin level was noted under constant darkness (DD) with an increase in TLC and LC of their neonates. The blastogenic response (BGR) to Con A of splenocytes exhibited a significant increase in neonates of SDL females and a significant decrease in the neonates of DD females. Hence, it appears that the increase in maternal plasma melatonin under SOL condition transmitted information to decrease the immune status. Continuous exposure of females to darkness (DD) negatively regulated the maternal pineal gland activity thereby decreasing their plasma melatonin level. This information was transmitted for elevation of immune status in neonates, so that they exhibit better growth and sexual maturation. Therefore, we may suggest that the maternal photic information transmitted either prenatally through placenta or postnatally via the milk regulate the hormonal profile of Melatonin to regulate the immune status of neonates in order to influence their growth and sexual maturation.

Effect of raft and pile stiffness on seismic response of soil-piled raft-structure system

Rajib Saha,Sekhar C. Dutta,Sumanta Haldar 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.55 No.1

Soil-pile raft-structure interaction is recognized as a significant phenomenon which influences the seismic behaviour of structures. Soil structure interaction (SSI) has been extensively used to analyze the response of superstructure and piled raft through various modelling and analysis techniques. Major drawback of previous study is that overall interaction among entire soil-pile raft-superstructure system considering highlighting the change in design forces of various components in structure has not been explicitly addressed. A recent study addressed this issue in a broad sense, exhibiting the possibility of increase in pile shear due to SSI. However, in this context, relative stiffness of raft and that of pile with respect to soil and length of pile plays an important role in regulating this effect. In this paper, effect of relative stiffness of piled raft and soil along with other parameters is studied using a simplified model incorporating pile-soil raft and superstructure interaction in very soft, soft and moderately stiff soil. It is observed that pile head shear may significantly increase if the relative stiffness of raft and pile increases and furthermore stiffer pile group has a stronger effect. Outcome of this study may provide insight towards the rational seismic design of piles.

Maternal photic regulation of immune status in neonates of Indian palm squirrel Funambulus pennanti

R.Singh,K.S.Bishnupuri,C.Haldar 한국광과학회 2002 Journal of Photosciences Vol.9 No.2

Hepatoprotective activity of Indigofera aspalathoides extract against CCl₄-induced liver damage

Gupta, M.,Mazumder, U.K.,Haldar, P.K.,Manikandan, L.,Senthilkumar, G.P.,Kander, C.C. Kyung Hee Oriental Medicine Research Center 2004 Oriental pharmacy and experimental medicine Vol.4 No.2

The plant Indigofera aspalathoides are used by a large number of tribes in India for the treatment of various hepatic disorder. The methanol extract of Indigofera aspalathoides (MEIA) was evaluated for its effect on carbontetrachloride $(CCl_{4})$ induced liver damage. Biochemical parameters such as serum glutamine oxaloacetate trasaminase (SGOT), serum glutamine pyruvate transaminase (SGPT), serum alkaline phosphatase (ALP), total serum protein (TP), thiobarbituric acid reactive substances (TBRS) and glutathione content of the liver were estimated to assess liver function and metabolism. Biochemical observations suggest that methanol extract of Indigofera aspalathoides (MEIA) significantly restored the liver function and metabolism towards normal condition in $CCl_{4}$-induced hepatic damage.