Stand-alone ClpG disaggregase confers superior heat tolerance to bacteria

Lee, Changhan,Franke, Kamila B.,Kamal, Shady Mansour,Kim, Hyunhee,Lunsdorf, Heinrich,Jager, Jasmin,Nimtz, Manfred,Trc0c,ek, Janja,Jansch, Lothar,Bukau, Bernd,Mogk, Axel,Romling, Ute National Academy of Sciences 2018 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.115 No.2

<P><B>Significance</B></P><P>Severe heat stress causes massive protein loss by aggregation ultimately causing cell death. Cellular survival relies on protein disaggregation mediated by the Hsp70-ClpB (Hsp100) bichaperone system in most bacteria. <I>Pseudomonas aeruginosa</I> additionally codes for two stand-alone ClpG disaggregases, which had been acquired by horizontal gene transfer by the species and most abundant clone C strains, respectively. These ClpG disaggregases largely contribute to the resolution of protein aggregates to confer superior heat tolerance partially replacing the DnaK-ClpB system.</P><P>AAA+ disaggregases solubilize aggregated proteins and confer heat tolerance to cells. Their disaggregation activities crucially depend on partner proteins, which target the AAA+ disaggregases to protein aggregates while concurrently stimulating their ATPase activities. Here, we report on two potent ClpG disaggregase homologs acquired through horizontal gene transfer by the species <I>Pseudomonas aeruginosa</I> and subsequently abundant <I>P. aeruginosa</I> clone C. ClpG exhibits high, stand-alone disaggregation potential without involving any partner cooperation. Specific molecular features, including high basal ATPase activity, a unique aggregate binding domain, and almost exclusive expression in stationary phase distinguish ClpG from other AAA+ disaggregases. Consequently, ClpG largely contributes to heat tolerance of <I>P. aeruginosa</I> primarily in stationary phase and boosts heat resistance 100-fold when expressed in <I>Escherichia coli</I>. This qualifies ClpG as a potential persistence and virulence factor in <I>P. aeruginosa</I>.</P>

OPTIMIZED ENERGY MANAGEMENT CONTROL FOR THE TOYOTA HYBRID SYSTEM USING DYNAMIC PROGRAMMING ON A PREDICTED ROUTE WITH SHORT COMPUTATION TIME

C. MANSOUR,D. CLODIC 한국자동차공학회 2012 International journal of automotive technology Vol.13 No.2

Among the general problematic of the HEV power trains, the most critical point is the determination of the power-split ratio between the mechanical and the electrical paths, known as the energy management strategy (EMS). Many EMS are proposed in the literature, and can be grouped in two categories: the local optimization EMS and the global optimization EMS. The local optimization category corresponds to the EMS based on human expertise and the knowledge of the power train components efficiency maps. Thus, the local optimization EMS manages the power train operations by referring to predefined rules. The drawback of such strategies is that it brings an instantaneous fuel consumption optimization, and does not fully optimize the fuel consumption over the whole trip. Therefore, additional fuel savings are still possible. This paper presents an overall optimized predictive EMS for the Toyota Hybrid System (THS-II) power train of the Prius. The proposed EMS is based on Dynamic Programming (DP), where the prior knowledge of the route is required in order to predetermine the power-split ratio and optimize the fuel consumption for the whole predicted route. The DP EMS proposed for the THS-II power train is designed with a very short computation time, intended to be implemented in real-time applications. The potential of this DP-controller in reducing fuel consumption on regulatory cycles are computed and compared to a rule-based controller and to the Prius published fuel consumption results. Finally, the fuel reduction enhancements of the DP-controller are computed for real road tests achieved on a MY06 Prius in Ile-de-France, by comparing to the associated observed consumption measurements.

DYNAMIC MODELING OF THE ELECTRO-MECHANICAL CONFIGURATION OF THE TOYOTA HYBRID SYSTEM SERIES/PARALLEL POWER TRAIN

C. MANSOUR,D. CLODIC 한국자동차공학회 2012 International journal of automotive technology Vol.13 No.1

The hybridization of the conventional thermal vehicles nowadays constitutes a paramount importance for car manufacturers, facing the challenge of minimizing the consumption of the road transport. Although hybrid power train technologies did not converge towards a single solution, series/parallel power trains with power-split electromechanical transmissions prove to be the most promising hybrid technology. In fact, these power trains show maximum power train overall efficiency and maximum fuel reduction in almost all driving conditions compared to the conventional and other hybrid power trains. This paper addresses the model and design of the electro-mechanical configuration of one of the most effective HEV power trains: case study of the 2nd generation Prius. It presents the simulation work of the overall operation of the Toyota Hybrid System (THS-II) of the Prius, and explores not only its power-split eCVT innovative transmission system but also its overall supervision controller for energy management. The kinematic and dynamic behaviors of the THS-II power train are explained based on the power-split aspect of its transmission through a planetary gear train. Then, the possible regular driving functionalities that result from its eCVT operation and the energy flow within its power train are outlined. A feedforward dynamic model of the studied power train is next proposed, supervised by a rule-based engineering intuition controller. The energy consumption of the THS-II proposed model has been validated by comparing simulation results to published results on European, American and Japanese regulatory driving cycles.

Standardized 4-step technique of bladder neck dissection during robot-assisted radical prostatectomy

Mansour Alnazari,Marc Zanaty,Emad Rajih,Assaad El-Hakim,Kevin C. Zorn 대한비뇨의학회 2016 Investigative and Clinical Urology Vol.57 No.-

Bladder neck (BN) dissection is considered one of the most challenging steps during robot-assisted radical prostatectomy. Better understanding of the BN anatomy, coupled with a standardized approach may facilitate dissection while minimizing complications. We describe in this article the 4 anatomic spaces during standardized BN dissection, as well other technical maneuvers of managing difficult scenarios including treatment of a large median lobe or patients with previous transurethral resection of the prostate. The first step involves the proper identification of the BN followed by slow horizontal dissection of the first layer (the dorsal venous complex and perivesicle fat). The second step proceeds with reconfirming the location of the BN followed by midline dissection of the second anatomical layer (the anterior bladder muscle and mucosa) using the tip of the monopolar scissor until the catheter is identified. The deflated catheter is then grasped by the assistant to apply upward traction on the prostate from 2 directions along with downward traction on the posterior bladder wall by the tip of the suction instrument. This triangulation allows easier, and safer visual, layer by layer, dissection of the third BN layer (the posterior bladder mucosa and muscle wall). The forth step is next performed by blunt puncture of the fourth layer (the retrotrigonal fascia) aiming to enter into the previously dissected seminal vesical space. Finally, both vas deferens and seminal vesicles are pulled through the open BN and handed to the assistant for upper traction to initiate Denovillier's dissection and prostate pedicle/neurovascular bundle control.

Recyclable single-stranded DNA template for synthesis of siRNAs

( Mussa M. Ali ),( Demian Obregon ),( Krishna C. Agrawal ),( Mahmoud Mansour ),( Asim B. Abdel Mageed ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.11

RNA interference is a post-transcriptional silencing mechanism triggered by the bioavailability and/or exogenous introduction of double-stranded RNA (dsRNA) into cells. Here we describe a novel method for the synthesis of siRNA in a single vessel. The method employs in vitro transcription and a single-stranded DNA (ssDNA) template and design, which incorporates upon self-annealing, two promoters, two templates, and three loop regions. Using this method of synthesis we generated efficacious siRNAs designed to silence both exogenous and endogenous genes in mammalian cells. Due to its unique design the single-stranded template is easily amenable to adaptation for attachment to surface platforms for synthesis of siRNAs. AsiRNA synthesis platform was generated using a 3` end-biotinylated ssDNA template tethered to a streptavidin coated surface that generates stable siRNAs under multiple cycles of production. Together these data demonstrate a unique and robust method for scalable siRNA synthesis with potential application in RNAi-based array systems. [BMB reports 2010; 43(11): 732-737]

Quantification of airway diameters and 3D airway tree rendering from dynamic hyperpolarized ³He magnetic resonance imaging

Lewis, Tina A.,Tzeng, Yang-Sheng,McKinstry, Erin L.,Tooker, Angela C.,Hong, Kwansoo,Sun, Yanping,Mansour, Joey,Handler, Zachary,Albert, Mitchell S. Wiley Subscription Services, Inc., A Wiley Company 2005 Magnetic resonance in medicine Vol.53 No.2

<P>As another step toward extracting quantitative information from hyperpolarized <SUP>3</SUP>He MRI, airway diameters in humans were measured from projection images and multislice images of the lungs. Values obtained were in good agreement with the Weibel lung morphometry model. The measurement of airway caliber can now be achieved without the use of ionizing radiation. Furthermore, it was demonstrated that 3D airway tree renderings could be constructed from the multislice data. Both the measurement of airway diameters and the rendering of 3D airway information hold promise for the clinical assessment of bronchoconstrictive diseases such as asthma and the associated evaluation of treatment effectiveness. Work is being done to address the uncertainties of the manually intensive methods we have developed. Magn Reson Med 53:474–478, 2005. © 2005 Wiley-Liss, Inc.</P>