Analysis of Methodology for Deriving the Total Amount of SKB Cellulose-Containing Radioactive Waste

Bora Yang,Jaechul Ha,Myunggoo Kang,Seho Choi 한국방사성폐기물학회 2022 한국방사성폐기물학회 학술논문요약집 Vol.20 No.2

Radioactive waste containing cellulosic materials such as cotton, paper and wood are being disposed in Low-and intermediate-level radioactive waste disposal site in Gyeongju. Cellulose has recently emerged great issue in terms of disposal site safety as it can be decomposed into an organic complex compound, ISA (isosaccharinic acid), under strong alkali conditions (pH 12.5 or higher) formed by the hydrated cement, to accelerate the mobility of the radionuclides in the disposal facility. However, in Korea, there are insufficient criteria for confirming the suitability for disposal of low-and intermediatelevel radioactive wastes including cellulose, and there is no specific method for evaluating the total amount of waste to confirm the suitability of disposal. Therefore, the method of SKB (Swedish Nuclear and Fuel Management Company), which has established acceptance criteria related to the physicalchemistry safety of cellulose, is analyzed to suggest a method for deriving the amount of cellulosecontaining waste disposal. Cellulose, an organic complexing agent, is an important consideration for safety case at the Swedish low-and intermediate-level radioactive waste disposal site SFR. SKB calculated the amount of cellulose generated by separately labeling cellulose-containing wastes of 1-2BMA, Silo and 1BTF (SKB 2013). BLA, a low-level radioactive waste disposal facility, is not considered due to its low radionuclide inventory (~0.2% of SFR’s total radionuclide inventory, SKB 2013). To calculate the amount of cellulose that can be disposed of, information on the mass and volume of hydrated cement (concrete waste, cement solidification waste, disposal container, grouting, disposal shed), the concentration of ISA absorbed in the hydrated cement, and the concentration of ISA dissolved in the groundwater which were used. In addition, the total disposable amount was calculated using the cellulose degradation rate, composition ratio, and the cellulose containing waste volume.

Multimodal evaluation of an interphotoreceptor retinoid-binding protein-induced mouse model of experimental autoimmune uveitis

Yang Jee Myung,Yun KyungA,Jeon Jehwi,Yang Hae Young,Kim Bora,Jeong Sunhong,Lee Junyeop,Oh Wang-Yuhl,Uemura Akiyoshi,Song Joon Seon,Kim Pilhan,Lee Joo Yong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

We aimed to characterize the vascular phenotypes of an experimental autoimmune retinal uveitis (EAU) model induced by interphotoreceptor retinoid-binding protein (IRBP) using multimodal imaging techniques. We systemically administered IRBP or vehicle to adult C57BL/6 mice. Fundus photography, optical coherence tomography (OCT), in vivo live confocal imaging using different tracers, OCT angiography (OCTA), and electroretinography (ERG) were performed after IRBP immunization. Hematoxylin and eosin and immunofluorescence staining were performed to characterize the immune response and vascular permeability. Mice with EAU exhibited perivascular inflammation, vitritis, and superficial retinal inflammation on fundus photography and OCT. H&E revealed immune cell infiltration in the perivascular area of the retina and choroid accompanied by a significant degree of perivasculitis that subsequently damaged photoreceptors 3 weeks postimmunization. Immunofluorescence staining showed subsequent transcytosis induction after local microglial activation followed by neutrophil recruitment in the perivascular area. Transcytosis in the superficial and deep vascular areas was improved by immune cell suppression. Intravital in vivo confocal imaging showed signs of neutrophil infiltration and obstructive vasculitis with perivascular leakage 3 weeks postimmunization. OCTA revealed a significant decrease in vascular flow in the deep capillary layer of the retina. Functional analysis showed that scotopic responses were intact at 2 weeks; however, normal photopic and scotopic responses were hardly detected in mice with EAU mice at 3 weeks postimmunization. Our data suggest that inflammatory cell activation and subsequent transcytosis induction in endothelial cells might be a major pathogenic factor for vascular leakage in uveitis, providing new insights into the pathophysiology of retinal vasculitis in noninfectious uveitis.

Long-term effects of human induced pluripotent stem cell-derived retinal cell transplantation in Pde6b knockout rats

Yang Jee Myung,Chung Sunho,Yun KyungA,Kim Bora,So Seongjun,Kang Seoon,Kang Eunju,Lee Joo Yong 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Retinal degenerative disorders, including age-related macular degeneration and retinitis pigmentosa (RP), are characterized by the irreversible loss of photoreceptor cells and retinal pigment epithelial (RPE) cells; however, the long-term effect of implanting both human induced pluripotent stem cell (hiPSC)-derived RPE and photoreceptor for retinal regeneration has not yet been investigated. In this study, we evaluated the long-term effects of hiPSC-derived RPE and photoreceptor cell transplantation in Pde6b knockout rats to study RP; cells were injected into the subretinal space of the right eyes of rats before the appearance of signs of retinal degeneration at 2–3 weeks of age. Ten months after transplantation, we evaluated the cells using fundus photography, optical coherence tomography, and histological evaluation, and no abnormal cell proliferation was observed. A relatively large number of transplanted cells persisted during the first 4 months; subsequently, the number of these cells decreased gradually. Notably, immunohistochemical analysis revealed that the hiPSC-derived retinal cells showed characteristics of both RPE cells and photoreceptors of human origin after transplantation. Functional analysis of vision by scotopic electroretinogram revealed significant preservation of vision after transplantation. Our study suggests that the transplantation of hiPSC-derived retinal cells, including RPE cells and photoreceptors, has a potential therapeutic effect against irreversible retinal degenerative diseases. Retinal degenerative disorders, including age-related macular degeneration and retinitis pigmentosa (RP), are characterized by the irreversible loss of photoreceptor cells and retinal pigment epithelial (RPE) cells; however, the long-term effect of implanting both human induced pluripotent stem cell (hiPSC)-derived RPE and photoreceptor for retinal regeneration has not yet been investigated. In this study, we evaluated the long-term effects of hiPSC-derived RPE and photoreceptor cell transplantation in Pde6b knockout rats to study RP; cells were injected into the subretinal space of the right eyes of rats before the appearance of signs of retinal degeneration at 2–3 weeks of age. Ten months after transplantation, we evaluated the cells using fundus photography, optical coherence tomography, and histological evaluation, and no abnormal cell proliferation was observed. A relatively large number of transplanted cells persisted during the first 4 months; subsequently, the number of these cells decreased gradually. Notably, immunohistochemical analysis revealed that the hiPSC-derived retinal cells showed characteristics of both RPE cells and photoreceptors of human origin after transplantation. Functional analysis of vision by scotopic electroretinogram revealed significant preservation of vision after transplantation. Our study suggests that the transplantation of hiPSC-derived retinal cells, including RPE cells and photoreceptors, has a potential therapeutic effect against irreversible retinal degenerative diseases.

Medicinal Chemistry : RESEARCH ARTICLE ; Cytotoxic 5α, 8α-epidioxy sterols from the marine sponge Monnanchora sp

( Bora Mun ),( Wei Hong Wang ),( Hi Young Kim ),( Dong Yup Hahn ),( In Ho Yang ),( Dong Hwan Won ),( Eun Hee Kim ),( Ji Hye Lee ),( Chul Kyeong Han ),( Hyun Ji Kim ),( Merrick Ekins ),( Sang Jip Nam ) 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-

Three new sterols, 5α, 8α-epidioxy-24-norcho-lesta-6.9(11), 22-trien-3β-ol (1), 5α, 8α-epidioxy-cholesta-6.9(11), 24-trien-3β, 25-diol (3), with four known sterols (4-7) were isolated from a marine sponge Monanchora sp, Their chemical structures were elucidated by extensive spectro-scopic analysis. Compounds 1 and 3-7 showed moderate cytotoxicity against several human carcinoma cell lines including renal (A-498), pancreatic (PANC-1 and MLA paCa-2), and colorectal (HCT 116) cancer cell lines.

Sporosarcina jeotgali sp. nov., Sporosarcina oncorhynchi sp. nov., and Sporosarcina trichiuri sp. nov., Isolated from Jeotgal, a Traditional Korean Fermented Seafood

Yang Ah-In,Kim Bora,Joe Sung-Hong,Joe Hae-In,Choe Hanna,Kim Ki Hyun,Jun Min Ok,Shin Na-Ri 한국미생물학회 2024 The journal of microbiology Vol.62 No.4

Three novel, Gram-stain-positive, obligate aerobic, catalase- and oxidase-positive bacterial strains, designated B2O-1T, T2O-4T, and 0.2-SM1T-5T, were isolated from jeotgal, a traditional Korean fermented seafood. Strains B2O-1T, T2O-4T, and 0.2-SM1T-5T exhibited distinct colony colors, characterized by pink, yellow, and red opaque circular colonies, respectively. Phylogenetic analysis revealed that three strains formed a paraphyletic clade within the genus Sporosarcina and shared < 99.0% similarity with Sporosarcina aquimarina KCTC 3840T and Sporosarcina saromensis KCTC 13119T in their 16S rRNA gene sequences. The three strains exhibiting Orthologous Average Nucleotide Identity values < 79.3% and digital DNA-DNA hybridization values < 23.1% within the genus Sporosarcina affirmed their distinctiveness. Strains B2O-1T, T2O- 4T, and 0.2-SM1T-5T contained MK-7 as a sole respiratory menaquinone and A4α type peptidoglycan based on lysine with alanine, glutamic acid, and aspartic acid. The common polar lipids include diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Strain T2O-4T contained one unidentified phospholipid, whereas strain 0.2-SM1T-5T contained two unidentified phospholipids. Cellular fatty acid profiles, with C15: 0 anteiso as the major fatty acid, supported the affiliation of the three strains to the genus Sporosarcina. Based on the polyphasic characteristics, strains B2O-1T (= KCTC 43506T = JCM 36032T), T2O-4T (= KCTC 43489T = JCM 36031T), and 0.2-SM1T-5T (= KCTC 43519T = JCM 36034T) represent three novel species within the genus Sporosarcina, named Sporosarcina jeotgali sp. nov., Sporosarcina oncorhynchi sp. nov., and Sporosarcina trichiuri sp. nov., respectively.

Global Metabolomics and Targeted Steroid Profiling Reveal That Rifampin, a Strong Human PXR Activator, Alters Endogenous Urinary Steroid Markers

Kim, Bora,Moon, Ju-Yeon,Choi, Man Ho,Yang, Hyang Hee,Lee, SeungHwan,Lim, Kyoung Soo,Yoon, Seo Hyun,Yu, Kyung-Sang,Jang, In-Jin,Cho, Joo-Youn American Chemical Society 2013 Journal of proteome research Vol.12 No.3

<P>Activation of the pregnane X receptor (PXR) alters the expression of metabolic enzymes and transporters involved in the metabolism of xenobiotics and endobiotics. To identify endogenous biomarkers of PXR activation in humans, rifampin, a strong PXR activator, was administered to 12 healthy male subjects, and their urine was analyzed before and after rifampin administration. Ultraperformance liquid chromatography time-of-flight mass spectrometry (UPLC/QTOF–MS)-based global metabolomics and gas chromatography–mass spectrometry (GC–MS)-based profiling of 75 steroids were used to screen the urine samples. Global metabolomics revealed that hydroxytestosterone sulfate and glycochenodeoxycholate sulfate levels were significantly increased and that androsterone sulfate, dehydroepiandrosterone (DHEA) sulfate, and <I>p</I>-cresol sulfate levels were significantly decreased following rifampin administration compared with controls. Urinary steroid profiling showed that 16α-OH-androstenedione (16α-OH-A-dione), 16α-OH-DHEA, 7α-DHEA, 7β-DHEA, and 11β-OH-A-dione levels were increased, whereas DHEA, androsterone, etiocholanolone, estrone, β-cortolone, and allo-tetrahydrocortisone levels were decreased in the rifampin group. The analysis of the metabolic pathway and the metabolic ratio of steroids enabled the estimation of the induction of CYP1A/3A/7B/11B/2C and the inhibition of CYP17A/19A in response to PXR activation. These human urinary biomarkers may be useful for predicting the extent of PXR activation, monitoring the activity of DMEs, and anticipating drug–drug interactions in patients administered PXR-activating drugs.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2013/jprobs.2013.12.issue-3/pr301021p/production/images/medium/pr-2012-01021p_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/pr301021p'>ACS Electronic Supporting Info</A></P>

성격특성, 오프라인 활동, 정치성향, 삶의 만족도에 따른 SNS 이용

진보래(Borae Jin),양정애(JungAe Yang) 한국언론학회 2015 한국언론학보 Vol.59 No.5

헌 옷 리폼을 위한 서비스 플랫폼 디자인 제안

김보라(Bora Kim),손정임(Jeongim Son),송서현(Seohyeon Song),Sayaka Kubono,Reina Noguchi,Manaka Sato,양원석(Wonseok Yang),연명흠(Myeong-Hum Yeoun) 한국디자인학회 2020 Design Works Vol.3 No.1

설문조사 기반의 비스페놀 A 인체 노출인자 평가

위보라(Bora Wi),이건우(Geonwoo Lee),양지연(Jiyeon Yang),임영욱(Youngwook Lim) 환경독성보건학회 2017 한국독성학회 심포지움 및 학술발표회 Vol.2017 No.5

Antiproliferative Prenylated Xanthones and Benzophenones from the Roots of <i>Cudrania tricuspidata</i> in HSC-T6 Cells

Jo, Yang Hee,Shin, Bora,Liu, Qing,Lee, Ki Yong,Oh, Dong-Chan,Hwang, Bang Yeon,Lee, Mi Kyeong American Chemical Society and American Society of 2014 Journal of natural products Vol.77 No.11

<P>Four new prenylated xanthones, cudracuspixanthones A–D (<B>1</B>–<B>4</B>), two new prenylated benzophenones, cudracuspiphenones A (<B>5</B>) and B (<B>6</B>), and 11 known xanthones (<B>7</B>–<B>17</B>) were isolated from the roots of <I>Cudrania tricuspidata</I>. The absolute configurations of compounds <B>2</B>–<B>4</B> were deduced by the comparison of the calculated optical rotation values with the measured data. Compounds <B>1</B>, <B>5</B>, and <B>6</B> showed moderate antiproliferative activity on HSC-T6 cells with IC<SUB>50</SUB> values of 9.7, 3.3, and 7.1 μM, respectively. Compounds <B>2</B>–<B>4</B>, <B>10</B>, and <B>14</B>–<B>16</B> had weaker activity. Flow cytometric analysis suggested that compounds <B>1</B> and <B>5</B> inhibited HSC-T6 cell proliferation in part by inducing apoptosis.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2014/jnprdf.2014.77.issue-11/np5002797/production/images/medium/np-2014-002797_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np5002797'>ACS Electronic Supporting Info</A></P>