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Koo, Bon-Hyeock,Yi, Bong-Gu,Wang, Wi-Kwang,Ko, In-Young,Hoe, Kwang-Lae,Kwon, Young-Guen,Won, Moo-Ho,Kim, Young-Myeong,Lim, Hyun Kyo,Ryoo, Sungwoo Yonsei University, College of Medicine 2018 Yonsei medical journal Vol.59 No.3
<P><B>Purpose</B></P><P>Vascular smooth muscle cell (VSMC) proliferation induced by native low-density lipoprotein (nLDL) stimulation is dependent on superoxide production from activated NADPH oxidase. The present study aimed to investigate whether the novel arginase inhibitor limonin could suppress nLDL-induced VSMC proliferation and to examine related mechanisms.</P><P><B>Materials and Methods</B></P><P>Isolated VSMCs from rat aortas were treated with nLDL, and cell proliferation was measured by WST-1 and BrdU assays. NADPH oxidase activation was evaluated by lucigenin-induced chemiluminescence, and phosphorylation of protein kinase C (PKC) βII and extracellular signal-regulated kinase (ERK) 1/2 was determined by western blot analysis. Mitochondrial reactive oxygen species (ROS) generation was assessed using MitoSOX-red, and intracellular L-arginine concentrations were determined by high-performance liquid chromatography (HPLC) in the presence or absence of limonin.</P><P><B>Results</B></P><P>Limonin inhibited arginase I and II activity in the uncompetitive mode, and prevented nLDL-induced VSMC proliferation in a p21Waf1/Cip1-dependent manner without affecting arginase protein levels. Limonin blocked PKCβII phosphorylation, but not ERK1/2 phosphorylation, and translocation of p47phox to the membrane was decreased, as was superoxide production in nLDL-stimulated VSMCs. Moreover, mitochondrial ROS generation was increased by nLDL stimulation and blocked by preincubation with limonin. Mitochondrial ROS production was responsible for the phosphorylation of PKCβII. HPLC analysis showed that arginase inhibition with limonin increases intracellular L-arginine concentrations, but decreases polyamine concentrations. L-Arginine treatment prevented PKCβII phosphorylation without affecting ERK1/2 phosphorylation.</P><P><B>Conclusion</B></P><P>Increased L-arginine levels following limonin-dependent arginase inhibition prohibited NADPH oxidase activation in a PKCβII-dependent manner, and blocked nLDL-stimulated VSMC proliferation.</P>
담쟁이덩굴 에탄올 추출물 첨가에 따른 키토산의 항균 상승 효과
김민정 ( Min Jung Kim ),신서윤 ( Seo Yoon Shin ),구본근 ( Bon Guen Koo ),백구 ( Gu Baek ),김태준 ( Tae Joon Kim ),박제권 ( Jae Kweon Park ) 한국키틴키토산학회 2011 한국키틴키토산학회지 Vol.16 No.3
Synergic antimicrobial activities of chitosan and Ivy extracts were investigated against several antibiotic and non-antibiotic resistant microorganisms. The antimicrobial substance from Parthenocissus tricuspidata (tentatively named as Ivy) were extracted using ethanol. Especially synergic antimicrobial activity of chitosan with Ivy extracts was exhibited toward E. coli ATCC8739 and Salmonella typhimurium. Although further study about synergic antimicrobial mechanism of chitosan and Ivy extracts is need to get better understanding, these are allowable to use as antimicrobial agents in food reservation, hygienic conditioner, and an additive in feed for poultry.