Aspergilli에 대한 Penicillia의 Mycoparasitism에 관하여

鄭圭永,李馥權,申炳湜 건국대학교 1978 論文集 Vol.8 No.1

The parasitisms of one fungus on another has been recognized by mycologists for one hundred years. As early as 1865 and 1870, de Bary described and named Piptocephalis freseniana and Cicinnobolus cesati and recognized them as mycoparasites. In these experiments, the light and electron microscopic study of the host-parasite relationships of Penicillium rugulosum and Asperqillus spp was made to investigate pathological histology and anatomical structure and similarities and differences between the haustoria of mycoparasites and parasites of vascular plants. The hosts were identified to be Asperqillus flavus (A-1) and Aspergillus oryae (A-2) and the parasite to be Penicillum rugulosum. The parasite (Penicillum rugulosum) was isolated from the host by method of Berry & Barnett and inoculated and cultured on the Synthetic medium (Czapek'a Dox ager), so the parasite is non obligate. The haustoria of the mycoparasite, Penicillium rugulosum, on Aspergillis flavus are similar to those of parasitic fungi on vascular plants. An electron-dense sheath bounded by a membrane surrounds the haustroail wall. The sheath membrane appears to be the invaginated host ectoplast. No collar is formed around the haustorium by the host wall.

하지 절단자 보행의 운동역학적 분석

임규찬,박윤서,배성제,정철수,신인식,김복영 師範大學 體育硏究所 1994 서울大學校 體育硏究所論集 Vol.15 No.2

This study was conducted to investigate the biomechanical characteristics of the gait pattern in an amputee for the purpose of improving the proper gait posture and designing the prosthetic. The subjects were six males amputees and one normal person. The ground reaction force was measured using AMTI force platform. The conclusions were as follows; 1. In case of amputee group, the ground reaction force was revealed in specific figure according to position in which they used the prosthetic, whereas in case of normal person the ground reaction force was revealed in the figure of two humps in regard of vertical axis. 2. In case of amputee group, the ground reaction force in time between fight foot contact and left foot contact was deviated from a figure of rectangle, whereas in case of normal person the ground reaction force was revealed in the figure of rectangle. 3. In case of amputee group, the ground reaction force referred to both feet angle point of view was revealed in irregular figure, whereas in case of normal person the ground reaction force was revealed about 47。

멀리뛰기 발구름 동작시 아킬레스건각이 도약속도와 도약각도에 미치는 영향

김복영,배성제,신인식,정철수,임규찬 師範大學 體育硏究所 1992 서울大學校 體育硏究所論集 Vol.13 No.2

The purpose of this study was to investigate the effect of lower leg planting angle an on take-off motion of the long jump. The Achilles tendon angle defined as the angle between shank and calcaneus with projection to XY plane was introduced to interprete lower leg motion uring last foot touch for the flight phase of long jump. A typical high speed film analysis technique including DLT method was adapted to record and analyze total of nineteen long jumps performed by elite athletes at two different track and field events. The following were found that: 1. The relationship between touch-down velocity, take-off velocity, range of vertical velocity and flight length were significantly related each others as r=0.6383, 0.6395 respectively at t=0.01 level. 2. It was not possible to reveal the relationship between take-off angle and flight length. Due to highly consistent take-off angles shown between the long jumpers. 3. The angular displacement of the shank during the last foot contact just before flight phase was negatively related to the flight length(r= -0.6149). However, it was not possible to find the relationship between the aehilles tendon angle and flight length. 4. As the range of the body C.O.G lean angle increased the last step foot contact time significantly increased(r=0.8625). 5. The foot contact time of Korean elite long jumpers was somewhat longer than that shown by foreign Athletes. It is concluded that maintaining touch-down velocity during initial phase of foot touch and increasing take-off velocity would result optimal take-off angle and good record. Decrease in the angular displacement of the shank, foot contact duration, and the range of body C.O.G lean angle were found to be important to improve the flight length.

게이지용 스텐레스강의 충격인성과 내마모성에 미치는 열처리의 영향

金學信,李圭福,李宗炫,金相泰 전북대학교 전자산업개발연구소 1990 전자산업연구 Vol.1 No.-

In this study, the effect of heat treatments on the mechanical properties was investigated in martensitic stainless steel for gauge. The experimental results showed that the hardness was decreased but the impact value was increased with increasing tempering temperature after solution teatment. Therefore, in the view-point of the hardness and impact toughness, it is better to temper at low temperature, 250℃. In isothermal transformation treatment, the hardness in martempering treatment was higher, but the impact value was lower than in marquenching treatment. The wear resistance was propor-tional to the increase in hardness and the wear mechanism was changed from mechanical abrasive wear to adhesive wear with increasing speed change. The wear loss was increased almost linearly with increasing load and the wear resistance in martempering treatment was better than any other heat treatments.

Fe-30%Ni-0.25%C합금의 기계적 성질에 미치는 Ausforming처리 및 Marforming처리의 영향

朴豊玉,李圭福,金學信 全北大學校 1993 論文集 Vol.36 No.-

The effect of ausforming treatment and marforming treatment on the mechanical properties of Fe-30%Ni-0.25%C alloy was investigated. The results obtained in this paper are as follows ; 1) The strength of ausformed martensite was mainly increased because of work-hardening inherited from deformed austenite. The better ductility of ausformed martensite was due to ductile matrix formation of retained austenite and absense of transformed twin and high dislocation density. 2) The strength of marformed martensite was increased by the increment of dislocation density, crossing of transformed twin with deformed twin and the mutual crossing of deformed twin. 3) The ductility of marformed martensite was lower than that of ausformed martensite but the strength of marformed martensite was higher. From this result, marforming treatment is much better than ausforming treatment for the improvement of mechanical properties in Fe-30Ni-0.25%C alloy.

고액 상평형의 측정과 추산 연구

박소진,박영신,황태원,한규진,오종혁,원동복 忠南大學校 産業技術硏究所 2001 산업기술연구논문집 Vol.16 No.2

정적인 융점측정 방법으로 benzene+n-dodecane, cyclohexane+phenol, phenol+water, water+sulfolane 및 diethylcarbonate+nitrobenzene계에 대한 고액 평형을 측정하였다. 측정된 고액평형 데이터는 문헌값과 modified UNIFAC에 의해 예측된 값과 비교하였으며, 추산값과 실험값은 ±1.61K 범위에서 잘 일치하였다. 본 연구에서 고찰된 diethylcarbonate의 융점은 기존문헌치와 큰 편차를 보여 재검증이 필요하다. Solid-liquid equilibrium(SLE) data were measured for benzene+ n-dodecane, cyclohexane+phenol phenol+water, water+sulfolane, and diethylcarbonate+ nitrobenzene systems by using static melting point measuring method. Measured SLE data were compared with the literature values and also compared with the values predicted by modified UNIFAC(Dortmund) equation. The experimental results are in good agreements with the published and estimated values, and the average deviation between the experimental values and the calculated values is ±1.16K. The measured melting point of diethylcarbonate shows some difference from literature value, and it will be certified through other experimental data for different systems.

Rearrangement of the Mo/Fe/S Cluster under the Reducing Conditions; Alternative Synthesis of MoFe₄S₆(PEt₃)₄Cl Cluster from [(Cl₄-cat)Mo(MeCN)Fe₃S₄Cl₃](Et₄N

Shin, Bok-Kyu,Han, Jae-hong Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.1

Exceptionally high percentage of IPP synthesis by <i>Ginkgo biloba</i> IspH is mainly due to Phe residue in the active site

Shin, Bok-Kyu,Kim, Mihyang,Han, Jaehong Elsevier 2017 Phytochemistry Vol.136 No.-

<P><B>Abstract</B></P> <P>(<I>E</I>)-4-Hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) reductase (IspH, HDR or LytB) is an Fe/S enzyme catalyzing the reductive dehydroxylation of HMBPP to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) in the last step of methylerythritol phosphate (MEP) pathway. The MEP pathway is known to produce 4–6:1 ratio of IPP and DMAPP mixture by the last enzyme, IspH. Plant IspH in plastids follows same catalytic mechanism as others, but GbIspH (<I>Ginkgo biloba</I> IspH) was reported to produce a mixture of IPP and DMAPP in a ratio of 16:1. Present catalytic mechanisms of IspH involve a common allyl anion intermediate, and the intramolecular proton transfer to the allyl moiety is considered as the key reaction step determining the product between IPP and DMAPP. The F212 residue in plant IspH was found as a potential amino acid residue that could mediate the proton transfer to the allyl anion intermediate before the product release. In this report, catalytic function of GbIspH F212 residue (H74 in <I>E. coli</I>), especially during the product formation in the active site, was studied by means of site-directed mutation. The product ratio of IPP/DMAPP was measured as 6.5 ± 0.1 for F212H GbIspH, and the value was close to the reported bacterial IspH having His residue on that specific position. Along with the other F212Y mutant, of which ratio was determined as 10.9 ± 0.1, the results strongly support that the Phe residue in plant IspH is the key amino acid residue that allows exclusive production of IPP in plant chloroplast.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Biochemical mechanism of almost exclusive IPP production by GbIspH is proposed. </LI> <LI> IPP/DMAPP is 6.5 ± 0.1 for F212H GbIspH, and 10.9 ± 0.1 for F212Y GbHDR, respectively. </LI> <LI> F212 of GbIspH is the key residue altering the proton transfer to the allyl anion intermediate before the product release. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Enzymatic Biotransformation of Red Ginseng and the Compositional Change of Ginsenosides

Shin, Bok-Kyu,Park, Hye-Yeon,Han, Jae-Hong The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.5

Compositional changes in ginsenosides and crude saponin during enzymatic biotransformation of red ginseng were studied on three commercial polysaccharide-hydrolyzing enzymes, Pectinex, Termamyl, and Celluclast. Considerable enhancement of crude saponin production was observed with Pectinex, whereas hydrothermally processed black ginseng showed significant decrease. Celluclast-treated red ginseng contained five-fold higher concentration of protopanaxadiol-type ginsenoside Rd, with decrease of precursor Rb1. Red ginseng treated with Pectinex showed distinct increases of protopanaxatriol-type ginsenosides Re and Rg1.

Structure of Mixed-Anions Tris(2-pyridylmethyl)amine Mn Complex, TPAMnη²-NO₃)(η-CIO₄)

Shin, Bok-Kyu,Kim, Mi-Hyang,Han, Jae-Hong Korean Chemical Society 2007 Bulletin of the Korean Chemical Society Vol.28 No.3

Mononuclear mixed-anions Mn complex of TPAMn(η2-NO3)(η-ClO4), where TPA is tris(2-pyridylmethyl)-amine, has been synthesized and characterized. The neutral TPAMn(η2-NO3)(η-ClO4) was obtained from the reaction between Mn(NO3)2·4H2O and [H3TPA](ClO4)3 in MeOH. X-ray crystallographic structure of mononuclear TPAMn(η2-NO3)(η-ClO4) complex showed a seven-coordinated geometry with a tripodal tetradentate TPA, a terminal perchlorate and an η2-bound nitrate.