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- 저자 : Biswojit Debnath (검색결과 1 건)
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Muhammad Irshad,Bizhu He,Shuang Liu,Sangeeta Mitra,Biswojit Debnath,Min Li,Hafiz Muhammad Rizwan,Dongliang Qiu 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.5
Okra (Abelmoschus esculentus L.) belongs to the Malvaceae family and is a difficult species to manipulateand regenerate in vitro. In this study, factors that influence secretion of phenolic compounds, callus induction, andsubsequent regeneration in okra were optimized. Different concentrations of anti-browning additives, such as activatedcharcoal (AC) and citric acid (CA) were tested alone or in combination with ascorbic acid (AA) in MS medium toevaluate their ability to control phenolic secretion from hypocotyl and cotyledon explants. Among the anti-browningadditives, 200 mg・L-1AC and 10 mg・L-1CA +AA (1:1) were best at controlling phenolic secretion from hypocotyland cotyledon explants in callus induction medium. Hypocotyl explants were more suitable for callus initiation andorganogenesis than cotyledon explants. Moreover, among twelve types of media tested for callus culture, the additionof 0.5 mg・L-12,4-dicholorophenoxy acetic acid (2,4-D) and 1.5 mg・L-1benzyladenine (BA) to the growth media wasthe most effective for hypocotyl explants, whereas MS + 1.5 mg・L-1α-naphthylacetic acid (NAA) and 0.5 mg・L-16-benzyloaminopurine (BAP) was effective for cotyledon explants. Improved shoot regeneration was achieved with 2mg・L-1BAP and 0.1 mg・L-1indole butyric acid (IBA). The maximum number of strong and healthy roots wasobtained on media that included 2 mg・L-1IBA and 200 mg・L-1AC. Rooted plantlets were successfully acclimatizedin earthen pots, and the rooted plantlets had normal morphology and growth characteristics. The present study offersa potential solution for controlling phenolic secretion during okra regeneration using additives and evaluates themanipulation of plant growth regulators for effective regeneration of the Wufu okra cultivar.
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