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      저자 : Bapurao G., Bharate (검색결과 2 건)
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      • Electrochemical H<sub>2</sub>O<sub>2</sub> biosensor composed of myoglobin on MoS<sub>2</sub> nanoparticle-graphene oxide hybrid structure

        Yoon, Jinho,Lee, Taek,Bapurao G., Bharate,Jo, Jinhee,Oh, Byung-Keun,Choi, Jeong-Woo Elsevier 2017 Biosensors & bioelectronics Vol.93 No.-

        <P><B>Abstract</B></P> <P>In this research, the electrochemical biosensor composed of myoglobin (Mb) on molybdenum disulfide nanoparticles (MoS<SUB>2</SUB> NP) encapsulated with graphene oxide (GO) was fabricated for the detection of hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>). Hybrid structure composed of MoS<SUB>2</SUB> NP and GO (GO@MoS<SUB>2</SUB>) was fabricated for the first time to enhance the electrochemical signal of the biosensor. As a sensing material, Mb was introduced to fabricate the biosensor for H<SUB>2</SUB>O<SUB>2</SUB> detection. Formation and immobilization of GO@MoS<SUB>2</SUB> was confirmed by transmission electron microscopy, ultraviolet-visible spectroscopy, scanning electron microscopy, and scanning tunneling microscopy. Immobilization of Mb, and electrochemical property of biosensor were investigated by cyclic voltammetry and amperometric i-t measurements. Fabricated biosensor showed the electrochemical signal enhanced redox current as −1.86μA at an oxidation potential and 1.95μA at a reduction potential that were enhanced relative to those of electrode prepared without GO@MoS<SUB>2</SUB>. Also, this biosensor showed the reproducibility of electrochemical signal, and retained the property until 9 days from fabrication. Upon addition of H<SUB>2</SUB>O<SUB>2</SUB>, the biosensor showed enhanced amperometric response current with selectivity relative to that of the biosensor prepared without GO@MoS<SUB>2</SUB>. This novel hybrid material-based biosensor can suggest a milestone in the development of a highly sensitive detecting platform for biosensor fabrication with highly sensitive detection of target molecules other than H<SUB>2</SUB>O<SUB>2</SUB>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The electrochemical biosensor composed of the myoglobin on the graphene oxide (GO)-encapsulated molybdenum disulfide nanoparticles (MoS<SUB>2</SUB> NP) was developed for hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) detection. </LI> <LI> MoS<SUB>2</SUB> NP were encapsulated by GO (GO@MoS<SUB>2</SUB>) for the first time to induce the electrochemical signal enhancement. </LI> <LI> Fabricated electrochemical biosensor with GO@MoS<SUB>2</SUB> showed the stability and reproducibility. </LI> <LI> Fabricated electrochemical biosensor also showed the enhanced amperometric response current by detection of H<SUB>2</SUB>O<SUB>2</SUB>. </LI> </UL> </P>

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        Electrochemical nitric oxide biosensor based on amine-modified MoS<sub>2</sub>/graphene oxide/myoglobin hybrid

        Yoon, Jinho,Shin, Jae-Wook,Lim, Joungpyo,Mohammadniaei, Mohsen,Bharate Bapurao, G.,Lee, Taek,Choi, Jeong-Woo Elsevier 2017 Colloids and Surfaces B Vol. No.

        <P><B>Abstract</B></P> <P>Nitric oxide (NO) is one of the most important molecules in living things due to its role as a signaling molecule in influencing pathological and physiological mechanisms including neurotransmission. In this study, the electrochemical biosensor based on the amine-modified molybdenum disulfide nanoparticles (MoS<SUB>2</SUB>), graphene oxide (GO) and myoglobin (Mb) hybrid material (amine-modified MoS<SUB>2</SUB>/GO/Mb hybrid) is developed to achieve the accurate detection of NO with electrochemical signal improvement. For the first time, the synthesis of MoS<SUB>2</SUB> accompanying the amine-modification of the surface of MoS<SUB>2</SUB> is done to hybridize with GO efficiently through the short linkage. After the amine-modification of MoS<SUB>2</SUB>, it is enclosed with GO directly (amine-modified MoS<SUB>2</SUB>/GO). Then, Mb which can induce the reduction of NO is immobilized on the amine-modified MoS<SUB>2</SUB>/GO to fabricate the amine-modified MoS<SUB>2</SUB>/GO/Mb hybrid for NO detection. The prepared hybrid shows the signal improved redox properties relative to the result of the electrode prepared without hybrid. Furthermore, upon addition of NO, the electrode prepared with hybrid shows the improved amperometric response compared with that of the electrode without hybrid. This amine-modified MoS<SUB>2</SUB>/GO/Mb hybrid can be used in the development of the biosensor platform accompanying the electrochemical signal improvement and accurate detection of target materials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The nitric oxide biosensor was developed by amine-modified MoS<SUB>2</SUB>/GO/Mb hybrid. </LI> <LI> For the first time, synthesis of MoS<SUB>2</SUB> accompanying the amine-modification was done. </LI> <LI> The hybrid showed the increase of electrochemical signal and amperometric response. </LI> <LI> The hybrid showed high selectivity in nitric oxide detection. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

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