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RISS 인기검색어
- 검색키워드
- 저자 : Bahramikia Seifollah (검색결과 3 건)
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Antioxidant Efficacy of Nasturtium officinale Extracts Using Various In Vitro Assay Systems
Seifollah Bahramikia,Razieh Yazdanparast 사단법인약침학회 2010 Journal of Acupuncture & Meridian Studies Vol.3 No.4
Nasturtium officinale R. Br. (watercress), of the family Brassicaceae, has been long used as a home remedy or a medicinal plant by the people of southeastern Iran. The aim of this study was to investigate the antioxidant activity of N. officinale extract using various in vitro assay systems, including the ferric reducing antioxidant power and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonate) assays, 1,1-diphenyl-2-picrylhydrazyl, hydrogen peroxide, nitric oxide radical scavenging, and ferrous ion chelating activity, as well as the inhibitory effect on ferrous ion/ascorbate induced lipid peroxidation, in rat liver homogenate. The results revealed that N. officinale extract possesses potent reducing power in a ferric reducing antioxidant power assay, concentration-dependent scavenging ability on 2,2-azinobis 3-ethylbenzothiazoline-6-sulfonate, 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals,and hydrogen peroxide, as well as chelating ability on ferrous ions. Furthermore,N. officinale extract prevented thiobarbituric acid reactive substances formation in ferrous ion/ascorbate induced lipid peroxidation in rat liver homogenate in a dosedependent manner. In addition, this N. officinale extract had the phenolic and flavonoid contents of 96.2 mg gallic acid equivalents/g dried extract and 63.2 mg catechin equivalents/g dried extract, respectively. The cumulative results clearly indicate that N. officinale extract possesses potent antioxidant properties probably mediated through direct trapping of free radicals, reducing power, and also through metal chelating. Based on its antioxidative potential, N. officinale extract might find applications in the prevention of free radical-related diseases.
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Golshahi Fatemeh,Bahramikia Seifollah 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.2
Elevation of intracellular glucose levels results in formation of advanced glycation end products (AGEs) through nonenzymatic glycosylation in intra- and intercellular proteins, whereby the proteins lose their natural function. Reactive oxygen species are involved in the glycosylation process of proteins. Therefore, in this study, we investigated the antioxidant activity and inhibitory effect of organic fractions of Trachyspermum copticum against the formation of AGE compounds in a diabetic model under experimental conditions. The total phenol and flavonoid contents of crude extract and different fractions were measured by folin–ciocalteu and ammonium chloride, while their antioxidant activity was measured by scavenging DPPH radicals. The diabetic model was developed by glycation of bovine serum albumen under experimental conditions, and the extent of AGE formation was measured based on fluorescence absorption at excitation and emission wavelengths of 335 and 385 nm, respectively. Protein carbonyl oxidation (PCO) and thiol groups’ oxidation as markers of oxidative damage to proteins were also measured. Our results indicate that the ethyl acetate fraction of Trachyspermum copticum at different concentrations (10–500 μg/ml) has the high antioxidant activity. Furthermore, the fraction had the maximum inhibitory effect on formation of AGE compounds and reduction of protein oxidative degradation through reducing PCO formation and enhancing thiol groups. Accordingly, we can conclude that the ethyl acetate fraction has anti-glycation effects under in vitro conditions, which might be due to its high polyphenolic content and antioxidant properties.
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Somayeh Amraee,Seifollah Bahramikia 경희대학교 융합한의과학연구소 2019 Oriental Pharmacy and Experimental Medicine Vol.19 No.2
Aldose reductase (AR) is the key enzyme of the polyol pathway, which plays an important role in the pathogenesis of diabetic complications. AR inhibitors can be used as an important strategy in the treatment of diabetic complications. The aim of the present study was to investigate effect of different fractions of Salvia officinalis on the bovine lens aldose reductase activity. For this purpose, the phenolic and flavonoid contents, IC50 values of different fractions of the S. officinalis to neutralize the DPPH free radicals were first measured. Then, attempts were made to investigate the effect of these fractions on the AR enzyme activity. Results indicated that ethyl acetate fraction had the highest of phenolic and flavonoid contents by 412.6 ± 1.55 and 372.5 ± 6.47 mg/ml, respectively. Also, the ethyl acetate fraction showed the lowest IC50 content of 1.18 μg/ ml for scavenging of the free radicals and 9.25 μg/ml for the inhibition of AR activity. According to the Lineweaver–Burk plot, the ethyl acetate fraction acts as an uncompetitive enzyme inhibitor. These findings revealed that all fractions showed inhibitory effect on AR activity, where in ethyl acetate fraction it was found to be maximum which may be due to its high phenolic and flavonoid content.
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