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RISS 인기검색어
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- 저자 : Baek Dongwon (검색결과 38 건)
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A Role for Arabidopsis miR399f in Salt, Drought, and ABA Signaling
Baek, Dongwon,Chun, Hyun Jin,Kang, Songhwa,Shin, Gilok,Park, Su Jung,Hong, Hyewon,Kim, Chanmin,Kim, Doh Hoon,Lee, Sang Yeol,Kim, Min Chul,Yun, Dae-Jin Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.2
MiR399f plays a crucial role in maintaining phosphate homeostasis in Arabidopsis thaliana. Under phosphate starvation conditions, AtMYB2, which plays a role in plant salt and drought stress responses, directly regulates the expression of miR399f. In this study, we found that miR399f also participates in plant responses to abscisic acid (ABA), and to abiotic stresses including salt and drought. Salt and ABA treatment induced the expression of miR399f, as confirmed by histochemical analysis of promoter-GUS fusions. Transgenic Arabidopsis plants overexpressing miR399f (miR399f-OE) exhibited enhanced tolerance to salt stress and exogenous ABA, but hypersensitivity to drought. Our in silico analysis identified ABF3 and CSP41b as putative target genes of miR399f, and expression analysis revealed that mRNA levels of ABF3 and CSP41b decreased remarkably in miR399f-OE plants under salt stress and in response to treatment with ABA. Moreover, we showed that activation of stress-responsive gene expression in response to salt stress and ABA treatment was impaired in miR399f-OE plants. Thus, these results suggested that in addition to phosphate starvation signaling, miR399f might also modulates plant responses to salt, ABA, and drought, by regulating the expression of newly discovered target genes such as ABF3 and CSP41b.
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Cross-talk between Phosphate Starvation and Other Environmental Stress Signaling Pathways in Plants
Baek, Dongwon,Chun, Hyun Jin,Yun, Dae-Jin,Kim, Min Chul Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.10
The maintenance of inorganic phosphate (Pi) homeostasis is essential for plant growth and yield. Plants have evolved strategies to cope with Pi starvation at the transcriptional, post-transcriptional, and post-translational levels, which maximizes its availability. Many transcription factors, miRNAs, and transporters participate in the Pi starvation signaling pathway where their activities are modulated by sugar and phytohormone signaling. Environmental stresses significantly affect the uptake and utilization of nutrients by plants, but their effects on the Pi starvation response remain unclear. Recently, we reported that Pi starvation signaling is affected by abiotic stresses such as salt, abscisic acid, and drought. In this review, we identified transcription factors, such as MYB, WRKY, and zinc finger transcription factors with functions in Pi starvation and other environmental stress signaling. In silico analysis of the promoter regions of Pi starvation-responsive genes, including phosphate transporters, microRNAs, and phosphate starvation-induced genes, suggest that their expression may be regulated by other environmental stresses, such as hormones, drought, cold, heat, and pathogens as well as by Pi starvation. Thus, we suggest the possibility of cross-talk between Pi starvation signaling and other environmental stress signaling pathways.
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Identification of a novel signaling component in phosphate starvation responses from Arabidopsis
Hyun Jin Chun,Dongwon Baek,Cheol Woo Choi,Kyoung Hee Lee,Dae-Jin Yun,Min Chul Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Phosphorus is one of the macronutrients essential for plant growth and development, as well as crop productivity. Many soils around the world are deficient in phosphate (Pi) that plants can utilize. To cope with the stress of Pi starvation, plants have evolved many adaptive strategies, such as changes of root architecture and enhanced Pi acquisition form soil. To understand molecular mechanism underlying Pi starvation stress signaling, we characterized the activation-tagged mutant showing altered responses to Pi deficiency compared to wild type Arabidopsis and named hsp3 (hypersensitive to Pi starvation3). hsp3 mutant exhibits enhanced phosphate transporter activity, resulting in higher Pi content than wild type. However, in root architectural change under Pi starvation, hsp3 shows hyposensitive responses than wild type, such as longer primary root elongation, lower lateral root density. Histochemical analysis using hsp3 mutant expressing auxin-responsive DR5::GUS reporter gene, indicated that auxin allocation from primary to lateral roots under Pi starvation is aborted in hsp3 mutant. Molecular genetic analysis of hsp3 mutant revealed that the mutant phenotype is caused by the lesion in ENHANCED SILENCING PHENOTYPE4 (ESP4) gene whose function is proposed in mRNA 3’ end processing. Here, we propose that mRNA processing plays a crucial role in Pi homeostasis in Arabidopsis.
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Dongwon Choi,Hyejin Bang,Tae Hyun Baek,Yeonshin Kim,Sangdo Oh 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07
The purpose of this study is to examine how a “paring” of a sponsoring brand with wellmatched products in a branded content (e.g., branded entertainment content and/or commercial) possibly leverages consumers’ attitude toward the sponsoring brand. For instance, to maximize the perceived benefit and desirability of their product, Guinness previously portrayed their product along with foods, such as oysters, steaks, and cheese, that would all be much better if consumers enjoyed it with a bottle of Guinness. We refer this type of practice as a “paring strategy,” another form of Leveraged Marketing Communications (LMC), and aim to explore how such paring practice successfully benefits the brand through association. In this context, this study shows a) a proportion of a sponsoring brand and a paring product portrayed in a content (e.g., 20/80 vs. 80/20), b), the attribute of the branded content (desirability vs. feasibility orientation), c) the demonstration natures of the paring strategy (integrated vs. sequential) creates association between a sponsoring brand and matching products, and amplify the perceived benefits of the sponsoring product. Finally, this study aims to explore the moderating role of consumer’s general tendency of processing style (holistic vs. analytic) in the effect of paring strategy.
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Dongwon Choi,Hyejin Bang,Yeonshin Kim,Tae Hyun Baek,Sukki Yoon 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07
This research examines how price discount, message assertiveness, and national culture interact to influence consumers’ response to the recycling messages on product packages. Results showed that American consumers respond more negatively to assertive (vs. nonassertive) recycling messages when paying a full price for the product, but they respond more positively to assertive (vs. nonassertive) messages when paying a discounted price for the product. For South Korean consumers, price discount and message assertiveness do not influence their responses to recycling advertising.
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온도 성층화를 갖는 예혼합압축자기착화엔진에서 부스터 효과에 관한 수치해석
정동원(Dongwon Jeong),임옥택(Ocktaeck Lim),백영순(Youngsoon Baek) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.5
The method of thermal stratification in cylinder is known for reducing rate of pressure rise, but which has its limit to extend the higher-load operating region of HCCI engines, giving an output of insufficient IMEP. High fueling rate could be one way to get the enough IMEP but it could be occurring engine knock. This work investigates the potential of in-cylinder thermal stratification for reducing the rate of pressure rise in DME HCCI engines, and the coupling between thermal stratification and booster effect to get reduced rate of pressure rise as well as high IMEP. The computations were conducted using Senkin application of the CHEMKINII kinetics rate code, and kinetic mechanism for Di-Methyl Ether (DME). DME has unique 2 stage reaction called Low Temperature Reaction (LTR) and High Temperature Reaction (HTR). Due to the heat release from LTR, the thermal stratification of in-cylinder would be formed just before HTR with large temperature difference so the heat release from oxidation reaction dispersed through combustion process. Intensity of intake pressure is closely concerned in input calorie which means a higher IMEP would be appeared without high fueling rate. The study also shows that increasing the in-cylinder thermal width of the charge between each zone would allow the booster pressure to be raised from 0.1 ㎫ to 0. 18㎫ to get the higher IMEP as well as high thermal efficiency with low emissions.
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An Empirical Study on the effect of Mobile Application Spin-Off
Elia Rho,Dongwon Lee,Jiye Baek 한국경영정보학회 2019 한국경영정보학회 학술대회논문집 Vol.2019 No.11
This study examines the impact of a mobile application spinoff on the new companion application adoption and user engagement. We exploit a natural experiment in the launch of a new spun-off companion mobile application to identify the causal effect of spin-off on spun-off companion application adoption and user engagement. In our research context, a mobile advertisement company implemented the mobile application spin-off due to the new platform policy enacted. However, there also existed an application in this company which was not affected by the policy and continued the service without any change. By comparing mobile application usage in these two distinct setups, we demonstrate robust evidence that reveals the impact of the spin-off on spun-off mobile application adoption and user engagement of the application. Our results demonstrate that the spun-off companion application adoption induces users to use the application less frequently as measured by daily active usage. Surprisingly, we find that user engagement, measured by an advertisement click-through-rate, with the spun-off application is significantly increased.
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