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Chang, Seox2010,Yoon,Kim, Dongx2010,Bin,Ryu, Gyeong Ryul,Ko, Seungx2010,Hyun,Jeong, Inx2010,Kyung,Ahn, Yux2010,Bae,Jo, Yangx2010,Hyeok,Kim, Myungx2010,Jun Wiley Subscription Services, Inc., A Wiley Company 2013 Journal of cellular biochemistry Vol.114 No.4
<P><B>Abstract</B></P><P>Glucagon‐like peptide‐1 (GLP‐1) and its potent agonists have been widely studied in pancreatic islet β‐cells. However, GLP‐1 receptors are present in many extrapancreatic tissues including macrophages, and thus GLP‐1 may have diverse actions in these tissues and cells. Therefore, we examined the mechanism by which exendin‐4 (EX‐4), a potent GLP‐1 receptor agonist, inhibits lipopolysaccharide (LPS)‐induced iNOS expression in Raw264.7 macrophage cells. EX‐4 significantly inhibited LPS‐induced iNOS protein expression and nitrite production. However, Northern blot and promoter analyses demonstrated that EX‐4 did not inhibit LPS‐induced iNOS mRNA expression and iNOS promoter activity. Electrophoretic mobility shift assay (EMSA) showed that EX‐4 did not alter the binding activity of NF‐κB to the iNOS promoter. Consistent with the result of EMSA, LPS‐induced IκBα phosphorylation and nuclear translocation of p65 were not inhibited by EX‐4. Also, actinomycin D chase study and the promoter assay using the construct containing 3′‐untranslated region of iNOS showed that EX‐4 did not affect iNOS mRNA stability. Meanwhile, cycloheximide chase study demonstrated that EX‐4 significantly accelerated iNOS protein degradation. The EX‐4 inhibition of LPS‐induced iNOS protein was significantly reversed by adenylate cyclase inhibitors (MDL‐12330A and SQ 22536), a PKA inhibitor (H‐89) and PKAα gene silencing. These findings suggest that EX‐4 inhibited LPS‐induced iNOS expression at protein level, but not at transcriptional mechanism of iNOS gene and this inhibitory effect of EX‐4 was mainly dependent on cAMP/PKA system. J. Cell. Biochem. 114: 844–853, 2013. © 2012 Wiley Periodicals, Inc.</P>
Cho, Minx2010,Jeong,Jung, Eunx2010,Kyung,Jung, Yunx2010,Sook,Shin, Haex2010,Eun,Kim, Eunx2010,Kyong,Kim, Hyeonx2010,Chang,Choi, Younx2010,Hee,Song, Keunx2010,Bae John Wiley Sons Australia, Ltd 2018 GERIATRICS & GERONTOLOGY INTERNATIONAL Vol.18 No.6
<P>ConclusionsMost oral problems are not simply the outcome of aging, with oral hygiene management being the most important determining factor. Oral health problems can be prevented, and in order to improve the quality of life of older adults, attention must be paid to oral healthcare. Geriatr Gerontol Int 2018; 18: 943-949.</P>
Hong, Mi Jeong,Yoo, Seung Soo,Choi, Jin Eun,Kang, Hyox2010,Gyoung,Do, Sook Kyung,Lee, Jang Hyuck,Lee, Won Kee,Lee, Jaehee,Lee, Shin Yup,Cha, Seung Ick,Kim, Chang Ho,Lee, Eung Bae,Cho, Sukki,Jheon, S John Wiley and Sons Inc. 2018 Cancer Science Vol.109 No.12
<P>RegulomeDB is a new tool that can predict the regulatory function of genetic variants. We applied RegulomeDB in selecting putative functional variants and evaluated the relationship between these variants and survival outcomes of surgically resected non‐small‐cell lung cancer. Among the 244 variants studied, 14 were associated with overall survival (<I>P </I><<I> </I>0.05) in the discovery cohort and one variant (rs2257609 C>T) was replicated in the validation cohort. In the combined analysis, rs2257609 C>T was significantly associated with worse overall and disease‐free survival under a dominant model (<I>P </I>=<I> </I>2 × 10<SUP>−5</SUP> and <I>P </I>=<I> </I>0.001, respectively). rs2257609 is located in the <I>SLC5A10</I> intron, but RegulomeDB predicted that this variant affected <I>DRG2</I>, not <I>SLC5A10</I> expression. The expression level of <I>SLC5A10</I> was not different with the rs2257609 genotype. However, <I>DRG2</I> expression was different according to the rs2257609 genotype (<I>P</I><SUB>trend</SUB><SUP> </SUP>= 0.03) and was significantly higher in tumor than in non‐malignant lung tissues (<I>P </I>=<I> </I>1 × 10<SUP>−5</SUP>). Luciferase assay also showed higher promoter activity of <I>DRG2</I> in samples with the rs2257609 T allele (<I>P </I><<I> </I>0.0001). rs2257609 C>T affected <I>DRG2</I> expression and, thus, influenced the prognosis of early‐stage non‐small‐cell lung cancer. This study was approved by the Institutional Review Broad of Kyungpook National University of Hospital (Approval No. KNUMC 2014‐04‐210‐003).</P>
Sohn, Young Bae,Kim, Su Jin,Park, Sung Won,Park, Hyungx2010,Doo,Ki, Changx2010,Seok,Kim, Chi Hwa,Huh, Seung Won,Yeau, Sunghee,Paik, Kyung‐,Hoon,Jin, Dongx2010,Kyu Wiley Subscription Services, Inc., A Wiley Company 2010 AMERICAN JOURNAL OF MEDICAL GENETICS PART A Vol.a152 No.12
<P><B>Abstract</B></P><P>Mucopolysaccharidosis type II (Hunter syndrome) is a lysosomal storage disease caused by a deficiency of iduronate‐2‐sulfatase. Most reported patients are males because of X‐linked recessive inheritance pattern. Only a few female patients with Hunter syndrome have been reported, and there is no prior report of offspring from a patient with Hunter syndrome. In this report, we describe a woman with mild manifestations of Hunter syndrome who gave birth to a daughter. Both the mother and daughter carried the p.R443X mutation in exon 9 of the <I>ID2S</I> gene. Iduronate‐2‐sulfatase activity in the mother was as low as that found in male Hunter syndrome patients, but it was in the low‐normal range in her daughter. Unlike her mother, the daughter did not show any physical signs of Hunter syndrome, and urinary excretion of glycosaminoglycan was within normal range. However, she had severe pulmonary vein stenosis with pulmonary hypertension and a large atrial septal defect and died at 11 months of age. © 2010 Wiley‐Liss, Inc.</P>
Yang, Hyun,Yoo, Yeongx2010,Min,Jung, Euix2010,Man,Choi, Kyung‐,Chul,Jeung, Euix2010,Bae Wiley Subscription Services, Inc., A Wiley Company 2010 Molecular reproduction and development Vol.77 No.11
<P><B>Abstract</B></P><P>Plasma membrane sodium/calcium exchangers are an important component of intracellular calcium homeostasis and electrical conduction. NCKX3 (gene <I>SLC24A3</I>), a potassium‐dependent sodium‐/calcium exchanger, plays a critical role in the transport of one intracellular calcium and potassium ion across the cell membrane in exchange for four extracellular sodium ions. <I>NCKX3</I> transcripts are most abundant in the brain and smooth muscle, but many other tissues, in particular, the uterus, aorta and intestine, also express this gene at lower levels. However, the expression and physiological roles of NCKX3 in the uterus of rats during the estrous cycle are unknown. Thus, we examined the uterine expression of NCKX3 mRNA and protein at different stages of the estrous cycle in mature and immature female rats in the absence or presence of the sex‐steroid hormones estrogen (E2) and progesterone (P4). During the estrous cycle, uterine expression of NCKX3 mRNA and protein was enhanced up to 4.0‐ and 2.5‐fold, respectively, at proestrus compared to during estrus and diestrus. To examine the effect of sex steroids on NCKX3 regulation in the uterus, immature female rats were treated with E2 (40 µg/kg body weight; BW), P4 (4 mg/kg BW), or E2 plus P4 for 3 days. The expression of NCKX3 mRNA and protein was induced by E2, whereas P4 antagonized E2‐induced NCKX3 expression. Subsequent immunohistochemical analysis revealed that uterine NCKX3 protein was abundantly localized in the cytoplasm of luminal and glandular epithelial cells throughout the estrous cycle. Taken together, these results indicate that uterine NCKX3 is abundantly expressed in the uterus and that its expression is regulated by the steroid hormones, E2 and P4. These findings suggest that NCKX3 may be involved in reproductive function during the estrous cycle in female rats. Mol. Reprod. Dev. 77:971–977, 2010. © 2010 Wiley‐Liss, Inc.</P>
Anti‐ageing effects of a new synthetic sphingolipid (K6EAA‐L12) on aged murine skin
Jung, Minyoung,Lee, Sanghoon,Park, Hwax2010,young,Youm, Jongx2010,Kyung,Jeong, Sekyoo,Bae, Jonghwan,Kwon, Mi Jung,Park, Byeong Deog,Lee, Seung Hun,Choi, Eung Ho Blackwell Publishing Ltd 2011 Experimental dermatology Vol.20 No.4
<P><B>Abstract: </B> Recently, we reported on the anti‐ageing effects of K6PC‐5. This compound induced keratinocyte differentiation and fibroblast proliferation by increasing sphingosine‐1 phosphate synthesis. We performed this study to confirm the anti‐ageing effects of new synthetic products (the K6EAA series) derived from K6PC‐5 through an amino group induction. Cellular responses such as differentiation, proliferation and calcium mobilization were investigated using cultured human keratinocytes and fibroblasts. Also, we measured the expressions of collagen mRNA and protein using real time RT‐PCR and ELISA, respectively. The K6EAA‐L12 product, selected by <I>in vitro</I> screening, was evaluated for anti‐ageing effects on intrinsically and extrinsically (photo) aged models of hairless mice. In the intrinsically aged murine skin, K6EAA‐L12 showed anti‐ageing effects by activating collagen synthesis, eventually causing dermal thickening. Also, in the photo‐aged skin, the dermal collagen density and dermal thickness were increased. In photo‐aged murine skin, K6EAA‐L12 increased stratum corneum integrity by increasing corneodesmosome density and improved the barrier recovery rate. However, there were no changes in the expressions of epidermal differentiation maker proteins. In conclusion, topical K6EAA‐L12, a new synthetic K6PC‐5 derivative, improves intrinsically and extrinsically (photo) aged skin by increasing the collagen density and improving the skin barrier function.</P>
Jung, Euix2010,Man,An, Beumx2010,Soo,Choi, Kyung‐,Chul,Jeung, Euix2010,Bae Wiley Subscription Services, Inc., A Wiley Company 2012 Journal of cellular biochemistry Vol.113 No.1
<P><B>Abstract</B></P><P>Calcium (Ca<SUP>2+</SUP>) is an important regulator of apoptotic signaling. Calbindin‐D<SUB>9k</SUB> (CaBP‐9k) and ‐D<SUB>28k</SUB> (CaBP‐28k) have a high affinity for Ca<SUP>2+</SUP> ions. Uterine calbindins appear to be involved in the regulation of myometrial activity by intracellular Ca<SUP>2+</SUP>. In addition, uterine calbindins are expressed in the mouse endometrium and are regulated by steroid hormones during implantation and development. The aim of the present study was to evaluate the regulation of apoptosis in the uteri of CaBP‐9k, CaBP‐28k, and CaBP‐9k/28k knockout (KO) mice. Our findings indicated that Bax protein was enhanced in the uteri of CaBP‐28k and CaBP‐9k/28k KO mice compared to wild‐type (WT) and CaBP‐9k KO mice, but no difference was observed in Bcl‐2 protein expression. The expressions of caspase 3, 6, and 7 proteins were higher in both CaBP‐28k and CaBP‐9k/28k KO mice than in WT and CaBP‐9k KO mice. These results suggest that the absence of CaBP‐28k increases apoptotic signaling. We also investigated the expression of endoplasmic reticulum (ER) stress genes by Western blot analysis in calbindin KO mice. C/EBP homologous protein and immunoglobulin heavy chain‐binding protein protein levels were elevated in CaBP‐28k KO mice compared to WT mice. When immature mice were treated with 17β‐estradiol (E2) or progesterone (P4) for 3 days, we found that the expressions of Bax and caspase 3 protein were increased by E2 treatment in WT and CaBP‐9k KO mice, and by P4 treatment in CaBP‐28k KO mice. These results indicate that CaBP‐28k blocks the up‐regulation of apoptosis‐related genes and ER stress genes, implying that CaBP‐28k may decrease the expression of genes involved in apoptosis and ER stress in murine uterine tissue. J. Cell. Biochem. 113: 194–203, 2012. © 2011 Wiley Periodicals, Inc.</P>
Bae, Kyung‐,Yul,Lim, Dongx2010,Hyuk,Park, Jix2010,Won,Kim, Hyunx2010,Joong,Jeong, Hanx2010,Mo,Takemura, Akio Wiley Subscription Services, Inc., A Wiley Company 2013 Polymer engineering and science Vol.53 No.9
<P><B>Abstract</B></P><P>Acrylic pressure sensitive adhesives (PSAs) have a range of applications in industry, such as medical products, aircraft, space shuttles, electrical devices, optical products, and automobiles, etc. In this study, acrylic PSAs with fluorinated groups were synthesized using 2,2,2‐trifluoroethyl methacrylate (TFMA) under UV radiation. The surface properties and adhesion strength were measured. The results showed that the addition of TFMA reduced the surface energy of the PSAs and improved the adhesion strength. POLYM. ENG. SCI., 2013. © 2013 Society of Plastics Engineers</P>
Hong, Mi Jeong,Lee, Shin Yup,Choi, Jin Eun,Kang, Hyox2010,Gyoung,Do, Sook Kyung,Lee, Jang Hyuck,Yoo, Seung Soo,Lee, Eung Bae,Seok, Yangki,Cho, Sukki,Jheon, Sanghoon,Lee, Jaehee,Cha, Seung Ick,Kim, C Wiley-Blackwells 2018 Thoracic cancer Vol.9 No.8
<P><B>Background</B></P><P>Genome‐wide association studies have indicated that most of the currently identified disease and trait‐associated single nucleotide polymorphisms (SNPs) are intronic or intergenic. RegulomeDB is a recently developed database that provides functional annotations for regulatory features of SNPs located in non‐coding regions. We evaluated the potential regulatory SNPs in the <I>EGFR</I> gene region using RegulomeDB and their associations with prognosis after surgery in non‐small cell lung cancer (NSCLC) patients.</P><P><B>Methods</B></P><P>A total of 698 patients with surgically resected NSCLC were enrolled and seven SNPs were selected based on the RegulomeDB database. All SNPs were genotyped using SEQUENOM MassARRAY iPLEX assay.</P><P><B>Results</B></P><P>Among the seven SNPs evaluated, rs9642391 (<I>EGFR</I> ivs19+2851C>G) was significantly associated with survival outcome (adjusted hazard ratio [HR] for overall survival = 0.70, 95% confidence interval [CI] 0.56–0.87, <I>P</I> = 0.001; adjusted HR for disease‐free survival = 0.82, 95% CI 0.70–0.97, <I>P</I> = 0.02; under a codominant model). According to RegulomeDB, rs9642391C>G, which is located in intron 19 of <I>EGFR</I>, was predicted to influence the expression of <I>GBAS</I> but not <I>EGFR</I>. As predicted, rs9642391C>G was associated with <I>GBAS</I> (<I>P</I> = 0.024) but not <I>EGFR</I> messenger RNA expression in tumor tissues.</P><P><B>Conclusion</B></P><P>In conclusion, our study provides evidence that rs9642391C>G in the intron of <I>EGFR</I> is associated with <I>GBAS</I> expression and survival outcomes of patients with surgically resected early‐stage NSCLC.</P>
Kim, Soochan,Han, Sinsuk,Withers, David R.,Gaspal, Fabrina,Bae, Jingyu,Baik, Song,Shin, Hyunx2010,Chool,Kim, Kyung‐,Su,Bekiaris, Vasileios,Anderson, Graham,Lane, Peter,Kim, Mix2010,Yeon WILEY‐VCH Verlag 2011 European journal of immunology Vol.41 No.6
<P><B>Abstract</B></P><P>Here, we identify cells within human adult secondary lymphoid tissues that are comparable in phenotype and location to the lymphoid tissue inducer (LTi) cells that persist in the adult mouse. Identified as CD117<SUP>+</SUP>CD3<SUP>−</SUP>CD56<SUP>−</SUP> cells, like murine LTi cells, they lack expression of many common lineage markers and express CD127, OX40L and TRANCE. These cells were detected at the interface between the B‐ and T‐ zones, as well as at the subcapsular sinus in LNs, the location where LTi cells reside in murine spleen and LNs. Furthermore, like murine LTi cells, these cells expressed high levels of IL‐22 and upregulated IL‐22 expression upon IL‐23 stimulation. Importantly, these cells were not an NK cell subset since they showed no expression of IFN‐γ and perforin. Interestingly, a subset of the CD117<SUP>+</SUP>CD3<SUP>−</SUP>CD56<SUP>−</SUP>OX40L<SUP>+</SUP> population expressed NKp46, again similar to recent findings in mice. Finally, these cells supported memory CD4<SUP>+</SUP> T‐cell survival in an OX40L‐dependent manner. Combined, these data indicate that the CD117<SUP>+</SUP>CD3<SUP>−</SUP>CD56<SUP>−</SUP>OX40L<SUP>+</SUP> cells in human secondary lymphoid tissues are comparable in phenotype, location and function to the LTi cells that persist within adult murine secondary lymphoid tissues.</P>